Researches on Fermentation Engineering of Polysaccharide of

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1 13 1 Vol13 No Life Science Research Feb a 1b, 2, : (Cordyceps militaris), :, 6% 1% 25, ; 6% 1% 22, : ; ; ; ; ; : TQ92 : A : (2009) Researches on Fermentation Engineering of Polysaccharide of Cordyceps militaris WANG Ju-feng 1 LI Hu-ming College of Bio-resources and Environmental Sciences Jishou University Jishou Hunan China 2 Research Centre for natural Pharmacy Jishou University Jishou Hunan China 3 Hunan TGL Bio-engineering Co LTD Changsha National Bio-industrial Base Changsha Hunan China Abstract The influence of temperature the concentration of glucose and protein on the biomass and the polysaccharide yield of Cordyceps militaris in cultural conditions were investigated The results showed that these factors greatly infected the biomass the yield of the extrocellular polysaccharide intracellular polysaccharide and total polysaccharide In this experiment when cultural temperature was 25 and with 6% of glucose and 1% of protein in the media the biomass the yield of total polysaccharide and extrocellular polysaccharide reached the highest and the yield of polysaccharide of the mycelium intracellular polysaccharide reached the highest in the following conditions 22 6% of glucose and 1% of protein Key words temperature carbon source nitrogen source Cordyceps militaris fermentation Cordyceps militaris polysaccharide Cordyceps militaris Lex Fr Link Life Science Research ~070 Eumycota Ascomycotina Pyrenomycetes Sphaeriales Clavicipitaceae Cordyceps [1] Cordyceps sinensis : [2004]99 04JJ Tel wangchenyu888@126com

2 d [2,3] 135~140 r / min 5 d [4~10] 1 L 9 (3 4 ) Table 1 The election of factors and levels in L 9 (3 4 ) Factors Levels a: C/% b: N/% c: Temperature/ d r / min 10 min d r/min 10 min 3 95% 24 h 1 11 Cordyceps militaris SIGMA3-18K 15 h r / min SW-CJ-2FD 10 min 10 HPG-400H 15 h 3 THZ-82B 95% 65%~70% HVE h AE ml Sevag 10% H 2 O % 24 h 100 ml ml nm W/V L % 25 ± 1

3 1 : K 1 K 2 K 3 k 1 k 2 k 3 2 Table 2 The results of data processing of the mycelium and polysaccharide of Cmilitaris Dry weight of mycelium Polysaccharides a: C/% b: N/% c: Temperature/ a: C/% b: N/% c: Temperature/ R Comparison in factors Best project c a b a 3 b 3 c a c b a 3 b 3 c K 1 1 K 2 2 K 3 3 k 1 k 2 k 3 K 1 K 2 K 3 R=max{K 1 K 2 K 3}-min{K 1 K 2 K 3} Notes K 1 is the sum total observed targets under 1st level K 2 is the sum total observed targets under 2nd level K 3 is the sum total observed targets under 3rd level k 1 k 2 k 3 is the mean value of K 1 K 2 K 3 respectively R=max{K 1 K 2 K 3}-min{K 1 K 2 K 3} K 1 K 2 K 3 k 1 k 2 k 3 R Table 3 Comparison in factors Best project 3 The results of data processing of the extracellular polysaccharide and intracellular polysaccharide of Cmilitaris a: C/% b: N/% c a b a 3 b 3 c 2 c: Temperature/ a: C/% b: N/% c b a a 3 b 3 c 1 c: Temperature/ K 1 1 K 2 2 K 3 3 k 1 k 2 k 3 K 1 K 2 K 3 R =max{k 1 K 2 K 3}-min{K 1 K 2 K 3} Notes K 1 is the sum total observed targets under 1st level K 2 is the sum total observed targets under 2nd level K 3 is the sum total observed targets under 3rd level k 1 k 2 k 3 is the mean value of K 1 K 2 K 3 respectively R =max{k 1 K 2 K 3}-min{K 1 K 2 K 3} g / L 9 2 R c a 6867 b 6694 c a b K a K 3 K b K 3 K c K 2 a 3 b 3 c 2 a 3 b 3 c 2 12~30 1 [11] 6% 1% 25 3~4 1

4 R c a b K c K 2 a 3 b 3 c [15] 25 2 [12] [13] [14] [16] 25 6% 1% 25 mycclium / g L Temperature / 1 Fig1 The relationship of temperature and the mycelium yield of Cmilitaris R polysaccharide / g L Total polysaccharide C / % 3 Fig3 The relationship of nitrogen source and the 2 Fig2 The relationship of carbon source and the extracellular polysaccharide the intracellular polysaccharide and the total polysaccharide content of Cmilitaris K K a K 3 K b K 3 C/N [17] 3 6% 1% g / L 25 polysaccharide / g L Total polysaccharide N / % extracellular polysaccharide, the intracellular polysaccharide, and the total polysaccharide content of Cmilitaris

5 1 : 69 polysaccharide / g L Total polysaccharide Temperature / 4 Fig4 The relationship of temperature and the extracellular polysaccharide, the intracellular polysaccharide, and the total polysaccharide content of Cmilitaris 23 1% 25 3 R c b a c b a K c K b K a K % a 3 b 3 c % 1% 22 a 3 b 3 c % 1% 25 2 [15] a 3 b 3 c 2 6% [18] 5 d 1% 24 2 a R c b 32 R a c b 3 K a 3 b 3 c 2 25

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