Acute and chronic toxicity of the methanolic extract of Ajuga iva in rodents
|
|
- Annis Watts
- 6 years ago
- Views:
Transcription
1 International Journal of Applied Research in Natural Products Vol. 9 (2), pp Directory of Open Access Journals IJARNP-HS Publication Original Research Acute and chronic toxicity of the methanolic extract of Ajuga iva in rodents Abdelouahab Diafat 1* ; Lekhmici Arrar 2* ; Yacine Derradji 2 ; Farid Bouaziz. 1 1 Laboratory of Characterization and Valorization of Natural Products, Faculty of Nature and Life Sciences, University Elbachir Elibrahimi, Bordj Bou Arreridj, Algeria. 2 Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif 1, Algeria. Summary. Ajuga iva, known as Chendgoura, is widely used in North African countries in the traditional medicine. However, there is little toxicological informations available regarding its safety following repeated exposure. The present investigation describes the toxicity of a methanolic extract of Ajuga iva. The administration of the Ajuga iva methanolic extract (AIME) at 2 14 g/kg of bodyweight (bwt) did not produce mortality or significant changes in the general behavior of mice. However, single intraperitoneal injections of AIME (2 6 g/kg bwt) produced a dose-dependent increase in adverse effects in the general behavior and the mortality rate. The LD 50 by intraperitoneal route was g/kg bwt. In chronic toxicological studies in rats, oral administration the AIME with daily doses of 100, 300 and 600 mg/kg bwt, did not cause any significant differences in the general conditions like growth, organ weights, hematological and biochemical parameters or in microscopic appearance of the organs (brain, liver and the kidney). In contrast, a transient rise in platelet counts and a decrease in serum glucose and cholesterol levels were noted. Therefore, the NOAEL for the AIME is 600 mg/kg/day administered orally for 13 weeks. So AI methanolic extract has low toxicity. Industrial relevance. The extract of the plant Ajuga iva can be used as herbal teas or for the treatment of several disease such as diabetes, high cholesterol and increased serum triglycerides. However, information regarding its toxicological properties is not available. In this study, we have shown that the methanolic extract of Ajuga iva has low toxicity in rodent models and may be considered for human use after further studies. Keywords. Ajuga iva; methanolic extract; acute toxicity; sub chronic toxicity INTRODUCTION Ajuga iva Schreiber, locally named Chendgoura, is a plant of the Lamiaceae family 1 (Figure 1). A. iva, develops in deep soil of 2700 m height. It grows in period from spring to late summer. The flowering period is between May and June 2,3. It is widely distributed in the Mediterranean region: southern Europe and northern Africa, particularly in Algeria, Morocco, Tunisia, and Egypt 3. The aerial part of the plant is used as an infusion or decoction in Algerian traditional medication to treat different diseases like diabetes and it is known to have an anti inflammatory, antifongic and anti-microbial effects 4. Some species of Ajuga as Ajuga shit were used for the cicatrization of the wounds and like an anti-inflammatory drug 5,6. Some studies proved that this plant presented a range of biological and pharmacological activities, which can justify its therapeutic use in the Algerian traditional medicine. The extract of the plant Ajuga iva can be used as herbal teas or capsules in the Pharmacy and Phytotherapy industries, which use dried plants, extracts or isolated active ingredients for the treatment of several disease such as diabetes, high cholesterol and increased serum triglycerides. *Corresponding Author. ilyes132@yahoo.fr (A. Diafat)/lekharrar@hotmail.com (L. Arrar) Available online http.// Figure 1. Photograph of Ajuga iva
2 Toxicity study of the methanolic extract of Ajuga iva Studies on the aqueous extract of the Ajuga iva indicated its hypoglycemic, vasorelaxant and hypolipidemic effects 7,8,9. Ajuga iva has anti-lipid peroxidation, antioxidant, antiarthritic 10, antifongic 11, antibacterial 12,13,14, anti hypertensive 15, antiplasmodial properties 16. Furthermore, It has an effect on enzymatic activities in diabetes 4,17, an antifeedant (insecticide) action against the larvae and insects 18,19,20. The ethanolic extract of Ajuga iva have shown an effective antifeedant activity against the frugiperda of Spodoptera and the littoralis S. 21. The traditional use of the Ajuga iva was also supported by the isolation and the identification of several possible active chemical components. The pharmacological activity of this plant is due mainly to all the flavonoids and, the polyphenol present in the sheet and the fruits. The principal compound in Ajuga iva were determined by Camps et al. 22. Four neoditerpenoids of clerodane (Ivain I - IV) were isolated from A. iva. Seven of the phytoecdysteroids were found in the whole plant, including the roots collected in Algeria 23. The studies of toxicity of Ajuga iva were carried out by El Hilaly et al. 24, who have found that the aqueous extract does not have any toxic effects, the LD 50 by intraperitonial track was 3.6 g / kg,. In this context and to confirm safety of the use of this plant, the present study was carried out on the extract obtained with the methanol, which is used for the extraction of the most polar molecules in order to provide conclusive scientific results for the sure use of this plant. MATERIALS AND METHODS Plant material. Ajuga iva (L.) Schreber was collected from Bordj Bou Arreridj, in the northeast of Algeria, and identified by Pr. H. Laouer (Department of Vegetal Biology and Ecology, University Ferhat Abbas, Setif 1). A voucher specimen was deposited at the Laboratory of Botany, Department of Vegetal Biology and Ecology (University Ferhat Abbas, Setif 1). All reagents were purchased from Sigma Chemicals (Germany), Fluka and Prolabo (France). Preparation of Ajuga iva extract. The extraction of polyphenols was carried out according to the method described by Markham 25. with slight modifications. Dried plant material was ground in warring blender, mixed with volumes of 85% aqueous methanol. The slurry was placed at room temperature for one week and the extract was filtered through a Buchner funnel and the methanol was removed by rotary evaporation using Buchi rotavapor. The dried extract was stored at -20 C temperature until use. Animals. Albino mice of the weight ranging between 23 and 25 g and Wistar rats of 8 weeks of age were used (provided by Institut Pasteur d Algérie). The animals were maintained for 7 days in the laboratory before the study. Mice or rats (5 males or 5 females) by group were placed in plastic cages with free access to food (ONAB, Bejaia) and water. They were maintained at 24 C under a cycle of 12 h of light/darkness. The animal studies were conducted after obtaining clearance from Institutional Animal Ethics Committee and the experiments were conducted in strict compliance according to ethical principles provided by Committee for the Purpose of Control and Supervision of Experiments on Animal (CPCSEA) Acute toxicity in the mice. Ajuga iva methanolic extract(aime) was dissolved in water and orally administered at a dose of 0, 2, 4, 6, 10 and 14g/kg of bodyweight (bwt) and intraperitoneally at a doses of 0, 2, 3, 4, 5, and 6 g/kg bwt (n = 10; 5 males and 5 females). The mice were fasted during the night before the administration of the extract. The general behavior of the mice was continuously monitored for the first hour after the administration, and periodically during the first 24 hours and every day for 14 days. At the end of the experiment all the animals were killed by chloroform inhalation, and the organs (liver, heart and brain) were taken, weighed and macroscopically examined 23,25. Sub-acute toxicity in the rats. AIME was dissolved in water and orally administered daily during 90 days (13 weeks) with amounts of 0, 100, 300, and 600 mg/kg bwt. All rats were anaesthetized with the chloroform at the end of each month and the blood samples were gathered by puncture of retro-orbital sinus in dry and EDTA containing tubes for biochemical and hematologic analyses, respectively 23,25.The hematologic analyses were carried out by a Coulter automate (laboratories of Abbott, IT, the United States), and biochemical tests were carried out using a COBAS Integra 800 (Rock,Germany. Histopathological examination. At the end of the experiment, rats were anesthetized with chloroform and sacrificed by decapitation, and then livers, brains and kidneys were carefully dissected out. Small slices of these freshly harvested tissues were fixed in buffered formaldehyde solution (10%), dehydrated by serial ethanol solution, diaphanized with ethanol benzene and enclosed with paraffin. Micrometer sections, cut by a microtome, were stained with hematoxylin eosin and examined under a light microscope then photomicrographs of the samples were recorded. Reproduction Test. The extract of Ajuga iva was administered at a dose of 600 mg/kg bwt to a group which contains (5 males and 5 Females). The administration in the males was carried out over a period of four weeks. This period covers two weeks minimal period before and during the period of coupling and an approximate period two weeks after the coupling. At the end of this period the rats were killed and the testicles were weighed, fixed in Bouin solution and analyzed for histopathological changes. In the females, the used dose was 600 mg/kg bwt throughout all the 40 days; two weeks before the coupling, the gestation period and at least four days after birth 26. During the period of the treatment, the animals were carefully observed at daily intervals to detect all signs of toxicity. The newborns were weighed and macroscopically observed on the signs of congenital malformation. Statistical analysis. All parameters were expressed as mean±(s.e.m.).. Statistical analysis was performed using Student s t-tests. P-values 0.05 were considered to be statistically significant. Analysis was done with OriginPro 9. RESULTS Acute toxicity. Among all treated animals, there was no death and no observed signs of toxicity after the oral administration of Ajuga iva extract on any level of doses until the highest doses of 14 g/kg bwt, which was considred as the dose no-observed- 10
3 Diafat et al. adverse-effect level (NOAEL). However, the death rate after the intraperitoneal administration of the methanolic extract is dose dependant. The death rate of 0% with doses 2 g/kg bwt, gradually reached 80% with 6 g/kg bwt, the highest dose in our study. The NOAEL for the intraperitoneal administration was 2 g/kg, whereas the low observed-adverse-effect level (LOAEL) was 3 g/kg. Some adverse effects, such as hypo-activity and diarrhea were observed just after the intraperitoneal injection, whereas others (anorexia and loss of the weight) were observed later, pronounced with the most raised dose and persisted until the death of the animals. The median lethal dose (LD 50 ) after intraperitoneal administration of the AIME in mice was 3980 mg/kg bwt (Figure 2). Figure 2. Dose-mortality curve for Ajuga iva methanolic extract in mice (single intraperitoneal doses). Calculated LD 50 = 3.98 g/kg Chronic toxicity studies in rats. Effect of AIME on the body weight and mortality. The body weight of the rats after the treatment by the AIME is presented in Figure 3. No significant difference in the profile of the body weight was noted between the treated groups and the control in any moment. Moreover, no mortality was recorded with any dose until the maximum of 600 mg/kg (NOAEL) during the 90 days of the treatment. Effect of AIME on the hematologic and biochemical parameters of rats. The effect of the chronic oral administration of AIME on the hematologic parameters is presented in Table 1. An increase of the numbers of the platelet and hemoglobin were observed in the treated groups in the 60 and 90 day of the treatment compared to the control group (p < 0.05). This increase was observed until the end of the treatment (90 days). All the other parameters (hematocrit, WBC and RBC) remained within the normal limits along the period of the treatment. The biochemical parameters of the treated and control rats are presented in Figure 4. The chronic oral administration of AIME (until an amount of 600 mg/kg bwt) did not cause crucial changes of creatinine and urea. However, the blood level of cholesterol, triglyceride and glucose were appreciably decreased in the treated animals (p < 0.05) compared to the control until the 90 days of treatment. The hypoglycemic effect was maintained at the end of the period of treatment (90 days). The activity of the enzymes (ALT, AST) were increased in the treated animals (p < 0.05) compared to the control. Rats treated with 600 mg/kg bwt dose of AIME showed marked centrolobular sinusoidal congestion (F2), compared to the control groups (F1), There was no effect on the kidneys of rats treated with 100, 300 and 600 mg/kg bwt dose of AIME, as the glomeruli, distal and proximal tubules appeared normal (R2), compared to the control group (R1). There was no effect on the brain of rats treated with 100, 300 and 600 mg/kg bwt of AIME (C2), compared to the control group (C1), (Figure 5). Effect on reproduction. After gestation, the new born were weighed and macroscopically observed. No difference was observed between treated and controls groups, concerning the weight of newborn, and the histological cuts of the testicles (Figure 6). Table 1: Effect of sub-chronic oral administration of Ajuga iva-extract on hematological parameters of rats. The methanolic extract of the plant was given daily by the oral route to groups of Wistar rats (n = 10 per group) at the following doses: 0 g/kg (GR4, control), 600 mg/kg (GR1), 300 mg/kg (GR2), and 100 mg/kg (GR4) for 90 days. Hematological parameters were measured after 30, 60 and 90 days of treatment. Data are expressed as mean±s.e.m. Days WBC 10 9 L plaq 10 9 L RBC L HT% HB g/l GR ± ± ± ± ± ± ± 58.7*** 9.93 ± ± ±
4 Toxicity study of the methanolic extract of Ajuga iva ± ± 27.3 * 9.65 ± ± ± 2.13** GR ± ± ± ± ± ± ± 173* 10.7 ± ± ± 0.98 * ± ± 166 * 9.67 ± ± ± 1.45 * GR ± ± ± ± ± ± ± ± ± ± 1.27 * ± ± ± ± ± 0.55 * GR ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.6 Figure 3. Effect of sub-chronic oral administration of Ajuga iva-extract on the body weight of rats. The plant extract was given daily by the oral route for 90 days to groups of Wistar rats (n = 10 per group) at the following doses: 0 g/kg (GR4, control), 600 mg/kg (GR1), 300 mg/kg (GR2), and 100 mg/kg (GR3). The rats were weighed before the start of AI-extract treatment (D0) and then every 15 days (D15, D30, D45, D60, D75 and D90). Data are expressed as mean ±S.E.M. 12
5 Diafat et al. Figure 4. Effect of sub-chronic oral administration of Ajuga iva extract on biochemical parameters of rats. The aqueous extract of the plant was given daily by the oral route to groups of Wistar rats (n = 10 per group) at the following doses: 0 g/kg (GR4, control), 600 mg/kg (GR1), 300 mg/kg (GR2), and 100 mg/kg (GR3) for 90 days. Biochemical parameters were measured after 30, 60 and 90 days of treatment. Panel A: glucose; Panel B: Urea, Panel C : creatinine ; Panel D: Cholesterol, Panel E : TG, Panel F: alanine aminotransferase (ALT); Panel G: aspartate aminotransferase (AST). Data are expressed as mean ± S.E.M.; (**) P < 0.01; (***) P < versus the control group. 13
6 Toxicity study of the methanolic extract of Ajuga iva Figure 5. photomicrographs of the liver (F1 F2), the brain(c1 C2), and the kidney (R1 R2); scale enlargement: 40. Photomicrographs of the sections of the liver showing normal features in control rats (F1), and the liver of rats treated orally with 600 mg/kg of AI-extract for 90 days showing a marked centrolobular sinusoidal congestion (F2). (R1) Photomicrographs of the sections of the kidney showing normal features in control rats and the kidney of rats treated orally with 600 mg/kg of AI-extract for 90 days (R2). (C1) Photomicrographs of the sections of the brain showing normal features in control rats and the brain of rats treated orally with 600 mg/kg of AI-extract for 90 days (C2). Figure 6. photomicrographs of testicular (T1-T2), scale enlargement: 40. (T1) Photomicrographs of the sections of the testicular showing normal features in control rats and the testicular of rats treated orally with 600 mg/kg of AI-extract for 30 days (T2). 14
7 Diafat et al. DISCUSSION El Hilaly et al. 24. Showed that the aqueous extract of Ajuga iva does not have any toxic effects. To confirm the safety of this plant, we have carried out a study of toxicity of methanolic extract which is used for the extraction of most polar molecules. In this study, during the evaluation of acute toxicity, no mortality was observed in after oral administration. In the daily evaluation of the clinic signs, after intraperitoneal administration, we have observed some changes such as hypo-activity, anorexia and the diarrheas only in the animals of the groups which were treated with doses more than 3 g/kg bwt. These changes were irreversible because they persisted until the end of the experiment. The lethal dose was LD 50 = 3980 mg/kg. it is near to that found in the first study done by El Hilaly et al 24. LD50 3.6g / kg, and according to the classification of Hodge et Sterner 26, the methanolic extracts of Ajuga iva was not toxic. In contrast, toxicity was observed in the intraperitoneal injection. thenonexistence of the harmful effects (toxic) after the oral administration of the methanolic extract of the Ajuga iva in the mice can be explained by the fall of the biodisponibility (low intestinal absorption or the effect of first hepatic passage) 24. In the chronic toxicity the daily clinical evaluation, no changes in the behavior were observed in the animals. In addition to these parameters, the changes of body weight are an indicator of the unfavorable side effects. In the present study no significant difference was found in the weight extended within the pre-established limits, justifying the selected doses p < Also, no significant change was observed in the consumption of water and food. The determination of such parameters is important in the study of the safety of a product with therapeutic aim. For the hematologic parameters, in the first group which received a dose of 600 mg/kg, the observation of an increase in the platelets and hemoglobin agrees with the result obtained by El Hillaly et al. 24. The other hematologic parameters did not show any significant changes. The analysis of blood parameters is appropriate to the toxicological evaluation of the risk because the hematologic system has a predictive value more raised for the human (91%) when the analyses imply rodents and not-rodent 27. In the biochemical evaluation, the obtained results showed a significant reduction in the glycemia until the end of the experiment. The same result was obtained in the study of El Hilaly et al. 24. For the other biochemical parameters, there were significant differences, of triglyceride and cholesterol in the treated groups compared to the control group. These results confirm the previous studies carried out by El Hilaly et al. 8 and Bouderbala et al. 17, respectively on the role of A. iva in the reduction of cholesterol and triglycerides. But in the study on A. iva aqueous extract, El Hilaly et al. 24 found normal rates of triglycerides and cholesterol. The other evaluated parameters (AST and ALT) are considered markers of the function of liver 28,24. The analysis of these parameters is important to evaluate the toxicity of the liver related to the use of the phytotherapeutic products 29,30. In the present study, some changes of these parameters have been observed, but they did not correlate with the administered doses. This must be confirmed by the histopathological examinations 28,24,31. The toxicity of kidney was also reported after the use of the phytotherapeutic products 32,33, what makes essential its evaluation. In this case, the determination of creatinine and urea were used as markers of the kidney function. In the present study, no significant difference in these parameters was detected. The studies carried out suggest that in the dose of 100 and 300 mg/kg (10 and 30 folds the indicated therapeutic dose), the product seems to be sure. However, the dose 600 mg/kg bwt, is much higher than that being usually used at human. Other studies in the non-rodents must be carried out to prove its safety. Moreover, the clinical use of Ajuga iva must be followed by a periodic hematologic tests. REFERENCES 1. Gonzalez-Tejero MR, Casares-Porcel CP, S anchez-rojas JM, Ramiro-Guti errez J, Molero-Mesa A, Pieroni M.E, et al. Medicinal plants in the Mediterranean area: Synthesis of the results of the project Rubia. Journal of Ethnopharmacology ; (116 ): Batanouny KH, Abou Tabl S, Shabana M. and Soliman F. Wild medicinal plants in egypt. Academy of Scientific Research and Technology. International Union for Conservation (IUCN). (1999).Egypt. 3. Halimi AK. Les plantes médicinales en Algérie. 1ère édition. BERTI Editions, Alger. 2004, pp: Taleb-Senoucia D, Ghomaria H, Kroufa D, Bouderbalaa S, Prostb J, Lacaille-Duboisc MA, Bouchenaka M. Antioxidant effectof Ajuga iva aqueous extractinstreptozotocin-induced diabetic rats Phytomedicine. 2009; (16): Enam AK, Fatma U, Afifi MAH. Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus) Journal of Ethnopharmacology. 2007; (109): El Beyrouthy M, Arnold N, Delelis-Dusollier A, Dupont F. Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon Journal of Ethnopharmacology ; (120): ElHilaly J, Lyoussi B. Hypoglycaemic effect of the lyophilised aqueous extract of Ajuga iva in normal and streptozotocin diabetic rats.journal of Ethnopharmacology. 2002; (80): ElHilaly J, Tahraoui A, Israili ZH, Lyoussi B. Hypolipidemic effects of acute and sub-chronic administration of an aqueous extract of Ajuga iva L. whole plant in normal and diabetic ratsjournal of Ethnopharmacology 2006; (105): Chenni A, Aityahia D, Boukortt FO, Prost J, Lacaille-Dubois A, Bouchenak M. Effect of aqueous extract of Ajuga iva supplementation on plasma lipid profile and tissue antioxidant status in rats fed a high-cholesterol diet. J.Ethnopharmacol ; (109): Arrar L, Diafat A, Charef N, Khennouf S, and Baghiani A. Preventive and curative effect of the methanolic extract of Ajuga iva on collagen induced arthritis in rats. Pharmacognosy Communications. 2013; (4): Kariba RM. Antifungal activity of Ajuga remota. Fitoterapia. 2001; (72); Chen H, Tan RX, Liu ZL, Zhang Y, Yang L. Antibacterial neoclerodane diterpenoids from Ajuga lupulina. Journal of Natural Products ; (59):
8 Toxicity study of the methanolic extract of Ajuga iva 13. Bennaghmouch L, Hajjaji N, Zellou A, Cherrah Y. Pharmacological study of Ajuga iva. Annales Pharmaceutiques Francaises. 2001; (59); Cantrell CL, Rajab MS, Franzblau SG, Fronczek FR, Fisher NH. Antimycobacterial ergosterol-5,8-endoperoxide from Ajuga remota. Planta Medica 1999; (65): Odek-Ogunde M, Rajab MS, Migwi GJ, Ndegwa JM. Blood pressure responses to an extract of Ajuga remota in experimentally hypertensive rats. Planta Medica. 1993; (59): Kuria KA, Muriuki G. A new cardiotonic agent from Ajuga remota Benth (Labiatae). East African Medical Journal 1984; (61): Bouderbala S, Lamri-Senhadjia M, Prostb J, Lacaille-Duboisc MA, Bouchenak M. Changes in antioxidant defense status in hypercholesterolemic rats treated with Ajuga iva Phytomedicine. 2008; (15): Bremner PD, Simmonds MSJ, Blaney WM, Veitch NC. Neo-clerodane diterpenoid insect antifeedants from Ajuga reptans ev Catlins Giant. Phytochemistry.1998; (47): Bondi ML, Al-Hillo MRY, Lamara K, Ladjel S, Bruno M, Piozzi F, Simmonds MSJ. Occurrence of the antifeedant 14,15- dihydroajugapitin in the aerial parts of Ajuga iva from Algeria. Industrial Crops and Products. 2000; (14): Ben Jannet H, Skhiri FH, Mighri Z, Simmonds MSJ, Blaney WM. Antifeedant activity of plant extracts and of new natural diglyceride compounds isolated from Ajuga pseudoi_a leaves against Spodoptera littoralis larvae. Industrial Crops and Products. 2001;(14): Simmonds MSJ, Blaney WM. Harley, R.M., Reynolds, T. (Eds.), Advances in Labiatae Science. 1992; Camps F, Coll J. Insect allelochemicals from Ajuga plants. Phytochemistry. 1993; (32): Wessner M, Champion B, Girault JP, Kaouadji N, Saidi B, Lafont R. Ecdysteroids from Ajuga iva. Phytochemistry 1992; (31): ElHilaly J, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals Journal of Ethnopharmacology 2004; (9) 1: Markham KR. Techniques of Flavonoid Identification. Academic Press, London Hodge HC. and Sterner JH.Determination of substances acute toxicity by LD50B. Amer. Industrial Hyg. Assoc.1943; (10): 93. In Lu EC. Toxicologie (traduit de l anglais par Lhuguenot JC et Riviere JL). Masson, paris Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A. Concordance of toxicity of pharmaceuticals in humans and in animals. Regulatory Toxicology and Pharmacology 2000; (32): Palmeiro NMS, Almeida CE, Ghedini PC, Goulart LS, Pereira MCF, Huber S, Silva JEP, Lopes S. Oral subchronic toxicity of aqueous crude extract of Plantago australis leaves. Journal of Ethnopharmacology. 2003; (88): Feres CAO, Madalosso RC, Rocha OA, Leite JPV, Guimaraes TM, Toledo VPP, Tagliati CA,. Acute and chronic toxicological studies of Dimorphandra mollis in experimental animals Journal of Ethnopharmacology. 2006; (108): Pittler MH, Ernst E. Systematic review: hepatotoxic events associated with herbal medicinal products. Alimentary Pharmacology and Therapeutics. 2003; (18): Kanjanapothi D, Panthog A, Lertprasertsuke N, Taesotikul T, Rujjanawatw C, Kaewpinit D, Sudthayakorn R, Choochote W, Chaithong U, Jitpakdi A, Pitasawat B. Toxicity of crude rhizome extract of Kaempferia galanga L. (Proh Hom). Journal of Ethnopharmacology. 2004; (90): Corns CM. Herbal remedies and clinical biochemistry. Annals of Clinical Biochemistry. 2003; (40): Isnard BC, Deray G, Baumelou A, Le Quintree M, Vanherweghem JL. Herbs and the kidney. American Journal of Kidney Diseases. 2004; (44):
African Journal of Pharmaceutical Research & Development Vol. 4 No.1 pp (2012)
African Journal of Pharmaceutical Research & Development Vol. 4 No.1 pp.16-19 (2012) Effects of Antimalarial Herbal Mixture (Abc 123) on the Liver of Rats *Iliya HA, Wannang NN, Andrew MM, Ibrahim H Department
More informationRepeated-Dose Dermal Toxicity of Topical Formulation of Hyptis suaveolens oil
CMU. Journal (2006) Vol. 5(3) 369 Repeated-Dose Dermal Toxicity of Topical Formulation of Hyptis suaveolens oil Kanokporn Niwatananun 1*, Wirat Niwatananun 1, Nirach Lertprasertsook 2 and Siriporn Okonogi
More informationANTI-DIABETIC ACTIVITY OF HELICTERES ISORA ROOT
ANTI-DIABETIC ACTIVITY OF HELICTERES ISORA ROOT Sama Venkatesh *1, G.Dayananda Reddy 1, B.Madhava Reddy 1, M.Lakshman 2. 1 Department of Pharmacognosy, G.Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(3):713-717 Effects of methanolic extract of Crataegus
More informationHYPOLIPIDEMIC EFFECT OF EXTRACTS FROM ABELMOSCHUS ESCULENTUS L. MALVACEAE ON TYLOXAPOL- INDUCED HYPERLIPIDEMIA IN MICE
HYPOLIPIDEMIC EFFECT OF EXTRACTS FROM ABELMOSCHUS ESCULENTUS L. MALVACEAE ON TYLOXAPOL- INDUCED HYPERLIPIDEMIA IN MICE Huynh Ngoc Trinh 1, Nguyen Ngoc Quynh 1, Tran T Van Anh 2, Vo Phung Nguyen 1 1 Department
More informationAcute and sub-acute toxicity studies of methanol leaf extracts of Annona squamosa linn. in mice
Sky Journal of Biochemistry Research Vol. 3(7), pp. 053 059, October, 2014 Available online http://www.skyjournals.org/sjbr ISSN 2315-8786 2014 Sky Journals Full Length Research Paper Acute and sub-acute
More informationInternational Journal of Medicine and Health Profession Research
Research Article ISSN: 2394 7403 International Journal of Medicine and Health Profession Research Journal home page: www.ijmhpr.com TOXICITY STUDY OF VAIVILANGAM CHOORANAM E. M. Manikgantan* 1 and R. Pattarayan
More informationGENERAL PRINCIPLES OF TOXICOLOGY
GENERAL PRINCIPLES OF TOXICOLOGY Laboratory of toxicology Department of Pharmacology and Toxicology College of Pharmacy, University of Baghdad 2015 TOXICOLOGY - PRACTICAL SYLLABUS 2015 General Principles
More informationToxicological Studies of the Aqueous Leaves Extracts of Combretum micranthum on Rats
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 12, Number 2 (2016) pp. 167-171 Research India Publications http://www.ripublication.com Toxicological Studies of the Aqueous
More informationStudy of the main chemical components of Ganoderma lucidum
Study of the main chemical components of Ganoderma lucidum Yasuo Komota et al Tokyo Medical and Dental University [Purpose] As part of the means for exerting quality control on Ganoderma lucidum 50% ethanol
More informationHYPOGLYCAEMIC ACTION OF THE FLAVONOID FRACTION OF ARTOCARPUS HETEROPHYLLUS LEAF
42 Research Paper ISSN 0189-6016 2006 Afr. J. Traditional, Complementary and Alternative Medicines www.africanethnomedicines.net HYPOGLYCAEMIC ACTION OF THE FLAVONOID FRACTION OF ARTOCARPUS HETEROPHYLLUS
More informationStudy of the main chemical components of Ganoderma lucidum
Study of the main chemical components of Ganoderma lucidum Yasuo Komota et al Tokyo Medical and Dental University [Purpose] As part of the means for exerting quality control on Ganoderma lucidum 50% ethanol
More informationENV 455 Hazardous Waste Management
Risk Assessment Basic Information ENV 455 Hazardous Waste Management Environmental Risk Assessment Özgür ZEYDAN (Phd.) http://cevre.beun.edu.tr/zeydan/ Hazard: a potential source of harm to a worker. Risk:
More informationANTI-DIABETIC ACTIVITY OF RIBES NIGRUM FRUIT EXTRACT IN ALLOXAN INDUCED DIABETIC RATS
IJPSR (2013), Vol. 4, Issue 3 (Research Article) Received on 30 November, 2012; received in revised form, 05 January, 2013; accepted, 27 February, 2013 ANTI-DIABETIC ACTIVITY OF RIBES NIGRUM FRUIT EXTRACT
More informationAntidiabetic effect of Teucrium polium aqueous extract in multiple low-dose streptozotocin-induced model of type 1 diabetes in rat
Antidiabetic effect of Teucrium polium aqueous extract in multiple low-dose streptozotocin-induced model of type 1 diabetes in rat Zari Sabet 1, Mehrdad Roghani 2*, Maryam Najafi 3, Zahra Maghsoudi 3 1.
More informationPharmacological and Toxicological study of Medicinally Important Plants: Solanum virginianum (kantakari)
Pharmacological and Toxicological study of Medicinally Important Plants: Solanum virginianum (kantakari) Submitted By Fatema Akther ID No. 2014-1-79-033 Department of Pharmacy East West University Research
More informationSummary of Inhalation Carcinogenicity Study. of Tetrachloroethylene. in BDF 1 Mice
Summary of Inhalation Carcinogenicity Study of Tetrachloroethylene in BDF 1 Mice March 1993 Japan Bioassay Laboratory Japan Industrial Safety and Health Association PREFACE The tests were contracted and
More informationEFFECT OF CULINARY MEDICINAL MUSHROOMS, PLEUROTUS OSTREATUS AND P. CYSTIDIOSUS ON FASTING AND POSTPRANDIAL GLYCAEMIA IN HEALTHY VOLUNTEERS
EFFECT OF CULINARY MEDICINAL MUSHROOMS, PLEUROTUS OSTREATUS AND P. CYSTIDIOSUS ON FASTING AND POSTPRANDIAL GLYCAEMIA IN HEALTHY VOLUNTEERS JAYASURIYA WJABN 1 *, WANIGATUNGE CA 2, FERNANDO GH 2, ABEYTUNGA
More informationAcute and subchronic toxicity study of the water extract from Tiliacora triandra (Colebr.) Diels in rats
Songklanakarin J. Sci. Technol. 30 (5), 611-619, Sep. - Oct. 2008 http://www.sjst.psu.ac.th Original Article Acute and subchronic toxicity study of the water extract from Tiliacora triandra (Colebr.) Diels
More informationACUTE AND SUB CHRONIC TOXICITY STUDY OF ETHANOL EXTRACT OF ANREDERA CORDIFOLIA (TEN.) V. STEENIS LEAVES
Innovare Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 6, Issue 5, 2014 Original Article ACUTE AND SUB CHRONIC TOXICITY STUDY OF ETHANOL EXTRACT OF
More informationThe Effects of Aframomum Melegueta Aqueous Extract on the Liver of Adult Wistar Rats
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article The Effects of Aframomum Melegueta Aqueous Extract on the Liver of Adult Wistar Rats Obike H I 1, Ezejindu D N 1, Akingboye
More informationTOXICOLOGICAL STUDIES ON AN ANTICANCER DRUG (HESA-A) WITH MARINE ORIGIN
Oncology TOXICOLOGICAL STUDIES ON AN ANTICANCER DRUG (HESA-A) WITH MARINE ORIGIN VALIOLLAH HAJHASHEMI* TAGHI GHAFGHAZI* MEHDI BALALI** AMROLLAH AHMADI* MASHOLLAH TAHER*** PARVIN RAJABI**** ARDESHIR TALEBI****
More informationSHRIRAM INSTITUTE FOR INDUSTRIAL RESEARCH
IN RATS SUB CHRONIC ORAL TOXICITY WITH NHH 44 Bt-COTTON SEEDS Report for: UNIVERSITY OF AGRICULTURAL SCIENCES AGRICULTURAL RESEARCH STATION DHARWAD-580007 KARNATAKA Guidelines: DBT, Guidelines for Toxicity
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(3):255-259 ISSN No: 0975-7384 CODEN(USA): JCPRC5 Analgesic and anti-inflammatory activities
More informationCOMMITTEE FOR VETERINARY MEDICINAL PRODUCTS
The European Agency for the Evaluation of Medicinal Products Veterinary Medicines Evaluation Unit EMEA/MRL/488/98-FINAL July 1998 COMMITTEE FOR VETERINARY MEDICINAL PRODUCTS DIETHYLENE GLYCOL MONOETHYL
More informationEVALUATION OF ACUTE AND SUB-ACUTE TOXICITIES OF GANODERMA LUCIDUM
EVALUATION OF ACUTE AND SUB-ACUTE TOXICITIES OF GANODERMA LUCIDUM Sheena.N Studies on the thekapeutic potential of Ganoderma lucidum P. Karst Reishi, occurring in Kerala Thesis. Amala Cancer Research Centre,
More informationANTIPLASMODIAL ACTIVITY OF CRUDE EXTRACTS AND ESSENTIAL OIL OF LIPPIA MULTIFLORA IN MICE
73 ANTIPLASMODIAL ACTIVITY OF CRUDE EXTRACTS AND ESSENTIAL OIL OF LIPPIA MULTIFLORA IN MICE 1 Malann, Yoila David*, 1 Deme, Gideon Gywa, 1 Daniel, Oluwatosin Esther, 1 Olanrewaju, Comfort Adetutu, 1 Olayanju,
More informationSAFETY AND HYPOGLYCAEMIC PROPERTIES OF AQUEOUS LEAF EXTRACT OF OCIMUM GRATISSIMUM IN STREPTOZOTOCIN INDUCED DIABETIC RATS.
Nigerian Journal Of Physiological Sciences 21 (1-2):31-35 Physiological Society Of Nigeria, 2006. 31 Available online/abstracted at http://www.biolineinternational.org.br/njps; www.ajol.info/journals.njps;
More informationBurak DiK 1, Emre BAHCIVAN 1,2, Hatice ESER 1,3, Kamil UNEY 1
Burak DiK 1, Emre BAHCIVAN 1,2, Hatice ESER 1,3, Kamil UNEY 1 1 Selcuk University Faculty of Veterinary Medicine, Pharmacology and Toxicology Department, Konya, TURKEY 2 Kafkas University Faculty of Veterinary
More informationInternational Journal of Research in Pharmacology & Pharmacotherapeutics
International Journal of Research in Pharmacology & Pharmacotherapeutics ISSN Print: 2278-2648 IJRPP Vol.3 Issue 4 Oct-Dec-2014 ISSN Online: 2278-2656 Journal Home page: Research article Open Access Anti-diabetic
More informationEvaluation of Diuretic Activity of Jussiaea Suffruticosa Linn.
Evaluation of Diuretic Activity of Jussiaea Suffruticosa Linn. R.Mythreyi 1, N.Rajkumar 1* and E.Sasikala 2 1 Maharaji College of Pharmacy, Besant Nagar, Chennai-90. 2 Central Research Institute for Siddha,
More information210 Vol 37 (suppl 3) 2006
Effect of Kaempferia parviflora Wall. Ex. Baker on Sexual Activity of Male Rats and its Toxicity Paiwan Sudwan 1,2, Kanokporn Saenphet 1, Supap Saenphet 1 and Songkiet Suwansirikul 2 1 Department of Biology,
More informationNutrition and Foods Safety Agency of the Centre for Disease Prevention and Control, People s Republic of China. Testing Report
Ministry Health approved Natural Health Products Testing Agency Issued by: Public Health Inspections, Ministry Health (1996) Publication # 53 Nutrition and Foods Safety Agency the Centre for Disease Prevention
More informationA. K. EYONG AND V. B. BRAIDE
GLOBAL JOURNAL OF MEDICAL SCIENCES VOL 8, NO. 1&2, 2009: 23-30 COPYRIGHT BACHUDO SCIENCE CO. LTD PRINTED IN NIGERIA. ISSN 1596-2911 www.globaljournalseries.com; Email: info@globaljournalseries.com EFFECTS
More informationEffects of some anti-diabetic plants on the hepatic marker enzymes of diabetic rats
Vol. 13(7), pp. 905-909, 12 February, 2014 DOI: 10.5897/AJB2013.13329 ISSN 1684-5315 2014 Academic Journals http://www.academicjournals.org/ajb African Journal of Biotechnology Full Length Research Paper
More informationRead Across with Metabolomics for Phenoxy Herbicides a Case Study with MCPP BASF SE
Read Across with Metabolomics for Phenoxy Herbicides a Case Study with MCPP BASF SE Prof. Dr. Bennard van Ravenzwaay, BASF, Ludwigshafen, Germany Experimental Toxicology and Ecology 1 Introduction: Case
More informationA high-performance liquid chromatography (HPLC) method for determination of chlorogenic acid and emodin in Yinhuang Jiangzhi Tea
Journal of Hainan Medical University 2016; 22(12): 17-21 17 Journal of Hainan Medical University http://www.jhmuweb.net/ A high-performance liquid chromatography (HPLC) method for determination of chlorogenic
More informationRESEARCH ARTICLE. Received on: 08/02/2017 Published on:27/03/2017
RESEARCH ARTICLE Received on: 08/02/2017 Published on:27/03/2017 Corresponding Author Anuradha VivekanandPhatak, Department of Pharmacology, Dr.BhanubenNanavati College of Pharmacy, Vile Parle (West),
More informationPharmacologyonline 1: (2009) HYPOGLYCEMIC EFFECT OF PHOEBE LA CEOLATA O ALLOXA -I DUCED DIABETIC MICE
HYPOGLYCEMIC EFFECT OF PHOEBE LA CEOLATA O ALLOXA -I DUCED DIABETIC MICE SEMWAL DK 1 *, RAWAT U 1, SEMWAL R 2, SINGH K 3, SINGH R 3, SAINI B 3, KRISHAN P 3, SINGH M 4 1 Department of Chemistry, University
More informationSAFETY ASPECTS OF MIDAZOLAM
Br. J. clin. Pharmac. (1983), 16, 37S-41S Biological Pharmaceutical Research Department, F. Hoffmann-La Roche & Co Ltd, CH-4002 Basle, Switzerland 1 The LD50 in the rat and the mouse is about 1600 mg/kg
More informationAcuteand Sub-Acute28-Day OralToxicityStudiesofEthanolicExtractofCeltisTimorensisLeavesin Rodents
: B Pharma, Drug Discovery, Toxicology and Medicine Volume 14 Issue 3 Version 1.0 Year 2014 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online
More informationN-Methylneodecanamide (MNDA)
United States Prevention, Pesticides EPA-730-F-99-008 Environmental Protection And Toxic Substances July 1999 Agency (7505C) New Pesticide Fact Sheet Description of the Chemical N-Methylneodecanamide (MNDA)
More informationResearch Article Anti hyperlipidemic Activity of Costus Igneus in Triton X- 100 Induced Hyperlipidemic Rats
Research Article Anti hyperlipidemic Activity of Costus Igneus in Triton X- 100 Induced Hyperlipidemic Rats Nimmy Chacko*, Shastry CS, Prerana shetty, Prasanna Shyamma, Ullas D souza and Patel Maulika
More informationEVALUATION OF ANTIDIABETIC ACTIVITY OF TRIUMFETTA PILOSA ROTH IN STREPTOZOTOCIN- INDUCED DIABETIC RATS
EVALUATION OF ANTIDIABETIC ACTIVITY OF TRIUMFETTA PILOSA ROTH IN STREPTOZOTOCIN- INDUCED DIABETIC RATS * D Ramakrishna 1, G Vidyasagar 2, K Pavan Kumar 1, I Madhusudhana Reddy 3, VSS S Gupta Atyam 4 1
More information298 Biomed Environ Sci, 2015; 28(4):
298 Biomed Environ Sci, 2015; 28(4): 298-302 Letter to the Editor Effects of Maternal Linseed Oil Supplementation on Metabolic Parameters in Cafeteria Diet-induced Obese Rats * BENAISSA Nawel 1, MERZOUK
More informationINTERNATIONAL JOURNAL OF PHYTOTHERAPY RESEARCH ISSN Research article
Research article EVALUATION OF SAFETY OF VETTUMARAN GUTIKA THROUGH SUB ACUTE TOXICITY STUDY IN WISTAR RATS Sanjaya Kumar Y.R*, Sushant S Parekar, Thamizh Selvam N, Sanal Gopi C.G and Sudarsanan Nair PK
More informationScholars Research Library J. Nat. Prod. Plant Resour., 2017, 7(2): (
Available online www.scholarsresearchlibrary.com Scholars Research Library J. Nat. Prod. Plant Resour., 2017, 7(2): 74-78 (http://scholarsresearchlibrary.com/archive.html) Evaluation of Anti-Inflammatory
More informationJustification for the selection of a candidate CoRAP substance
Justification for the selection of a candidate CoRAP substance Substance Name (Public Name): Diethyl phthalate Chemical Group: EC Number: 201-550-6 CAS Number: 84-66-2 Submitted by: Germany/Portugal Published:
More informationSupporting Information (SI) for: Renal Clearable Peptide Functionalized NaGdF 4 Nanodots for. High-Efficiency Tracking Orthotopic Colorectal Tumor in
Supporting Information (SI) for: Renal Clearable Peptide Functionalized NaGdF 4 Nanodots for High-Efficiency Tracking Orthotopic Colorectal Tumor in Mouse Hongda Chen, a,c Xiaodong Li, b Fuyao Liu, a Huimao
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences REVIEW ARTICLE PHARMACOLOGY A COMPARATIVE STUDY ON THE ANTIDIABETIC EFFECT OF NELUMBO NUCIFERA AND GLIMEPIRIDE IN STREPTOZOTOCIN INDUCED DIABETIC RATS POLURU
More informationSupplementary Information
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2017 Supplementary Information Geometrical Confinement Directed Albumin-Based
More informationTranslatability of cytokine data: from animals to humans. Marie-Soleil Piche, PhD Associate Scientific Director of Immunology Charles River, Montreal
Translatability of cytokine data: from animals to humans Marie-Soleil Piche, PhD Associate Scientific Director of Immunology Charles River, Montreal Presentation outline Overview of cytokines Factors related
More informationEvaluation of anti-arthritic potential of NONI extract in experimental rats
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationHepatotoxicity and subchronic toxicity tests of Morinda citrifolia (noni) fruit
Vol.34, No.5, 581-585, 2009 581 Letter Hepatotoxicity and subchronic toxicity tests of Morinda citrifolia (noni) fruit Brett J. West, Chen X. Su and C. Jarakae Jensen Research and Development, Tahitian
More informationScreening the Anti-ulcer Activity of Polyherbal Extract of Selected Medicinal Herbs Against Albino Wistar Rats
Original Article Screening the Anti-ulcer Activity of Polyherbal Extract of Selected Medicinal Herbs Against Albino Wistar Rats V. Swathi 1, Pusapati Madan Ranjit* 2, M.Ramesh 1 and Y.A. Chowdary 2 1 Division
More informationPharmacologyonline 3: (2009) I VESTIGATIO OF A TIHYPERGLYCEMIC EFFECT OF MORUS IGRA O BLOOD GLUCOSE LEVEL I STREPTOZOTOCI DIABETIC RATS
I VESTIGATIO OF A TIHYPERGLYCEMIC EFFECT OF MORUS IGRA O BLOOD GLUCOSE LEVEL I STREPTOZOTOCI DIABETIC RATS Hassan Fallah Hoseini 1, Soodabeh Saeidnia 2, Ahmad R. Gohari 2*, Mojgan Yazdanpanah 2, Abbass
More informationResearch on Extraction Process of Gallic Acid from Penthorum chinense Pursh by Aqueous Ethanol
Green and Sustainable Chemistry, 2015, 5, 63-69 Published Online May 2015 in SciRes. http://www.scirp.org/journal/gsc http://dx.doi.org/10.4236/gsc.2015.52009 Research on Extraction Process of Gallic Acid
More informationToxicology. Toxicity. Human Health Concerns. Health Effects of Hazardous Materials
Human Health Concerns Health Effects of Hazardous Materials Toxicology Study of the nature, effects, and detection of poisons in organisms Humans are obvious focal point Other species and ecosystem function
More informationSoursop (Anona muricata L.): Blood hematology and serum biochemistry of Sprague-Dawley rats
International Food Research Journal 19 (3): 955-959 (2012) Soursop (Anona muricata L.): Blood hematology and serum biochemistry of Sprague-Dawley rats 1,2 Syahida, M., 2 Maskat, M. Y., 1 Suri, R., 2* Mamot,
More information7. NOOTROPIC AND ANTIOXIDANT ACTIVITY. 7.1 Introduction
268 7. NOOTROPIC AND ANTIOXIDANT ACTIVITY 7.1 Introduction Alzheimer s disease is a neurodegenerative disorder, which according to World Health Organization (WHO) affects 22 million people world wide,
More informationComparison of toxic effects of Psidium guajava leaf and bark extracts against Brine Shrimp (Artemia salina)
International Journal of Agricultural Technology 2018 Vol. 14(7): 1033-1038 Available online http://www.ijat-aatsea.com ISSN: 2630-0613 (Print) 2630-0192 (Online) Comparison of toxic effects of Psidium
More information3. MATERIALS AND METHOD. 3.1 Collection and preparation of the plant material: The Murraya koenigii Spreng., leaves were collected from the
3. MATERIALS AND METHOD 3.1 Collection and preparation of the plant material: The Murraya koenigii Spreng., leaves were collected from the Department of Horticulture, Division of Aromatic and Medicinal
More informationTECHNICAL DATA SHEET ARNICA EXTRACT / AEC / HGL / CA20 /
ARNICA EXTRACT / AEC / HGL / CA20 / Trade Name: ARNICA EXTRACT / AEC / HGL / CA20 /. Product Code: CEARAHGLC20. INCI Name: AQUA, GLYCERIN, ARNICA MONTANA EXTRACT, POTASSIUM SORBATE, SODIUM BENZOATE. INCI
More informationThe Pharmaceutical and Chemical Journal, 2018, 5(3): Research Article
, 18, 5(3):65-71 Available online www.tpcj.org Research Article ISSN: 2349-792 CODEN(USA): PCJHBA Toxicological Effects of Saponin Extract from the Root of Bitter Kola (Garcinia kola) on the Biochemical
More informationBiochemical analysis of the crude extract of Momordica charantia (L.)
Biochemical analysis of the crude of Momordica charantia (L.) Ume Kalsoom Dar* 1, Farah Owais 2, Manzoor Ahmad 3 and Ghazala H. Rizwani 1 1 Department of Pharmacognosy, Research Institute of Pharmaceutical
More informationHypolipidemic Effect of Achillea biebersteinii Ethanolic Extract in Hamsters with Diet-Induced Hypercholesterolemia
Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2016; 8(9); 1555-1559 Research Article ISSN: 0975-4873 Hypolipidemic Effect of Achillea biebersteinii
More informationAntisickling and toxicological profiles of leaf and stem of Parquetina nigrescens L.
Journal of Medicinal Plants Research Vol. 4(8), pp. 639-643, 18 April, 2010 Available online at http://www.academicjournals.org/jmpr ISSN 1996-0875 2010 Academic Journals Full Length Research Paper Antisickling
More informationSEASONAL CHANGES OF AVOCADO LIPIDS DURING FRUIT DEVELOPMENT AND STORAGE
California Avocado Society 1968 Yearbook 52: 102-108 SEASONAL CHANGES OF AVOCADO LIPIDS DURING FRUIT DEVELOPMENT AND STORAGE Yoshio Kikuta Present address: Department of Botany, Faculty of Agriculture,
More informationInvestigation into the Nephrotoxicity of Nigerian Bonny Light Crude Oil in Albino Rats
Int. J. Environ. Res. Public Health 2004, 1(2), 106 110 International Journal of Environmental Research and Public Health ISSN 1660-4601 www.ijerph.org 2004 by MDPI Investigation into the Nephrotoxicity
More informationSUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix
SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix Dorota Woźniak A, Andrzej Dryś B, and Adam Matkowski* A A Department of Pharmaceutical Biology
More informationCHRONIC TOXICITY STUDY OF GARBHPAL RAS, AN AYURVEDIC MEDICINE
Journal of Herbal Medicine and Toxicology 3 (1) 13-17 (2009) ISSN : 0973-63 Original Article CHRONIC TOXICITY STUDY OF GARBHPAL RAS, AN AYURVEDIC MEDICINE D. MISHRA 1, M. SINHA 1, M. KUMAR 2 AND V. KUMAR
More informationResearch and Reviews: Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 232-1215 Research and Reviews: Journal of Pharmacy and Pharmaceutical Sciences Evaluation of Anti-Cancer Activity of Kigelia Africana on EAC Induced Breast Tumors. N Sri Sainadh*, Nagarathna PKM,
More informationAntimicrobial AlphaSan Test Report Summary Table 08/21/01
AlphaSan RC 5000 Physical Chemical Properties Acute Oral Toxicity, Rat EPA FIFRA 81-1 Acute Dermal Toxicity, Rat EPA FIFRA 81-2 Primary Dermal Irritation Rabbit EPA FIFRA 81-5 Primary Eye Irritation Rabbit
More informationEvaluation of Hepatoprotective Activity of Calotropis gigantea R.Br. Flowers
Ethnobotanical leaflets 14: 427-34, 2010. Evaluation of Hepatoprotective Activity of Calotropis gigantea R.Br. Flowers Ameeta Argal and Abhishek Dwivedi* *Correspondence Author e-mail: abhiherbal@gmail.com
More informationTOXICITY OF Acalypha torta (MUELL) LEAVES ETHANOLIC EXTRACT IN MICE AND RAT
1315 TOXICITY OF Acalypha torta (MUELL) LEAVES ETHANOLIC EXTRACT IN MICE AND RAT 1 EZEKWESILI, Chinwe Nonyelum, 2 OBIDOA, Onyechi, 3 NWODO, Okwesili Fred Chiletugo and 4 EZEKWESILI-OFILI, Josephine Ozioma
More informationANTI-INFLAMMATORY EFFECT OF Myrtus nivellei Batt & Trab (MYRTACEAE) METHANOLIC EXTRACT. M. Touaibia * and F.Z. Chaouch
Journal of Fundamental and Applied Sciences ISSN 1112-9867 Available online at http://www.jfas.info ANTI-INFLAMMATORY EFFECT OF Myrtus nivellei Batt & Trab (MYRTACEAE) METHANOLIC EXTRACT M. Touaibia *
More informationToxicological Assessments of Piper nigrum on Alloxan Induced Diabetic Rats
American-Eurasian Journal of Toxicological Sciences 6 (2): 30-34, 2014 ISSN 2079-2050 IDOSI Publications, 2014 DOI: 10.5829/idosi.aejts.2014.6.2.8490 Toxicological Assessments of Piper nigrum on Alloxan
More informationAsian Journal of Pharmaceutical Education and Research Vol -7, Issue-3, July-September 2018 ISSN:
Asian Journal of Pharmaceutical Education and Research Vol -7, Issue-3, July-September 2018 ISSN: 2278 7496 RESERCH ARTICLE Impact Factor: 5.019 ANTI ANEMIC ACTIVITY OF FRUIT OF PRUNUS DOMESTICA IN PHENYLHYDRAZINE
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(3):154-158 In vivo hepatoprotective effect of Trianthema
More informationDetection of Hypoglycemic and Antidiabetic Fraction in Ethanol Extract of Jatropha curcas Aerial Parts
Pharmacology & Pharmacy, 2014, 5, 663-669 Published Online June 2014 in SciRes. http://www.scirp.org/journal/pp http://dx.doi.org/10.4236/pp.2014.57076 Detection of Hypoglycemic and Antidiabetic Fraction
More informationEffect of Ethanol Leaf-Extract of Annona Muricata on Liver Enzymes of Albino Rats
IOSR Journal of Nursing and Health Science (IOSR-JNHS) e-issn: 23 1959.p- ISSN: 23 194 Volume 4, Issue 5 Ver. III (Sep. - Oct. 15), PP 99-13 www.iosrjournals.org Effect of Ethanol Leaf-Extract of Annona
More informationDietary supplementation in treating non-alcoholic fatty liver disease Dr. Ahmad Saedi Associate Professor School of Nutritional Sciences and
Dietary supplementation in treating non-alcoholic fatty liver disease Dr. Ahmad Saedi Associate Professor School of Nutritional Sciences and Dietetics Tehran University of Medical Sciences Honorary Academic
More informationTHE PHARMA INNOVATION - JOURNAL Chemoprotective effect of ethanolic extract of Morinda citrifolia against Cisplatin induced nephrotoxicity
Received: 29-12-2013 Accepted: 27-02-2014 ISSN: 2277-7695 CODEN Code: PIHNBQ ZDB-Number: 2663038-2 IC Journal No: 7725 Vol. 3 No. 1. 2014 Online Available at www.thepharmajournal.com THE PHARMA INNOVATION
More informationUnderstanding Blood Tests
PATIENT EDUCATION patienteducation.osumc.edu Your heart pumps the blood in your body through a system of blood vessels. Blood delivers oxygen and nutrients to all parts of the body. It also carries away
More informationBiochemical And Hematological Study On Butanol Fraction Of Leaves Of Moringa Stenopetala In Experimental Rats.
IOSR Journal Of Pharmacy (e)-issn: 2250-3013, (p)-issn: 2319-4219 Volume 6, Issue 5 (May 2016), PP. 64-68 www.iosrphr.org Biochemical And Hematological Study On Butanol Fraction Of Leaves Of Moringa Stenopetala
More informationPharmacologyonline 1: (2009)
EVALUATION OF SAPONIN RICH FRACTION OF TRIGONELLA FOENUM GRAECUM FOR ANTIHYPERTENSIVE ACTIVITY Arun Theerthahalli Sudheendra 1, Rekha Shenoy 2, Ashok D Taranalli 3 1 Lecturer, Dept of Pharmacology MCOPS,
More informationAcute and sub-acute (28-days) oral toxicity studies of Eraippu noi chooranam
International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com Volume 3, Issue 6-2016 Research Article Acute and sub-acute (28-days) oral toxicity studies of Eraippu noi
More informationDr. Ranjit Kumar. Scientist I Mahavir Cancer Institute & Research Centre, Phulwarisharif, Patna (Bihar), India
Dr. Ranjit Kumar Scientist I Mahavir Cancer Institute & Research Centre, Phulwarisharif, Patna (Bihar), India Diabetes is a global epidemic and, according to the International Diabetes Federation, one
More informationSubchronic Oral Toxicity Study of Morinda citrifolia (Mengkudu) in Spraque Dawley Rats
Pertanika J. Trop. Agric. Sci. 34 (2): 341-349 (2011) ISSN: 1511-3701 Universiti Putra Malaysia Press Subchronic Oral Toxicity Study of Morinda citrifolia (Mengkudu) in Spraque Dawley Rats Rosly, S.M.
More informationBasic Biopharmaceutics, Pharmacokinetics, and Pharmacodynamics
Basic Biopharmaceutics, Pharmacokinetics, and Pharmacodynamics Learning Outcomes Define biopharmaceutics Describe 4 processes of pharmacokinetics Describe factors that affect medication absorption Describe
More informationWound healing activity of Kaempferia rotunda Linn leaf extract
ISSN: 2319-7706 Volume 2 Number 12 (2013) pp. 74-78 http://www.ijcmas.com Original Research Article Wound healing activity of Kaempferia rotunda Linn leaf extract Syed Aman Imam, Saroj Kumar Rout, Niranjan
More informationAsian Journal of Biomedical and Pharmaceutical Sciences 1 (3) 2011, 26-31
Asian Journal of Biomedical and Pharmaceutical Sciences 1 (3) 2011, 26-31 RESEARCH ARTICLE ISSN 2249-622X Hepatoprotective Potential of Abutilon Hirtum Sweet Leaves In Carbon Tetrachloride Induced Hepatotoxicity.
More informationSTUDY ON STORAGE CONDITION INFLUENCE UPON THE NITRITE CONTENT IN PARSLEY
STUDY ON STORAGE CONDITION INFLUENCE UPON THE NITRITE CONTENT IN PARSLEY Eufrozina Albu*, Alina-Mihaela Pşibilschi Faculty of Food Engineering, Stefan cel Mare University of Suceava, Romania, *E-mail:
More informationSpermicidal activity of the hexane extract of Piper longum: An in vitro study
SUPPLEMENTARY MATERIAL Spermicidal activity of the hexane extract of Piper longum: An in vitro study Abu Hasnath Md Golam Sarwar a, b, Ranjeet Kumar Nirala a, b, Mohammed Arif a, Beena Khillare b, Sonu
More informationEFFECT OF DYKURE A POLYHERBAL FORMULATION ON TYPE-I AND TYPE-II DIABETES
EFFECT OF DYKURE A POLYHERBAL FORMULATION ON TYPE-I AND TYPE-II DIABETES Address for Correspondence: Harshul Parikh a, R. Balaraman b Prof. R. Balaraman Pharmacy Department Faculty of Tech. & Engg., The
More informationPharmacologyonline 3: (2009)
THE EFFECT OF ASTURTIUM OFFICIALE O BLOOD GLUCOSE LEVEL I DIABETIC RATS Hassan Fallah Hoseini 1, Ahmad R. Gohari 2, Soodabeh Saeidnia 2 *, aghi Shahabi Majd 3, Abbass Hadjiakhoondi 2 1- Department of Pharmacology,
More informationAcute and Subacute Toxicity Studies of the Methanol Extract from Leaves of Pisonia Aculeata LINN.
Research Article Shweta P. Ghode 1 *, B. Rajkapoor 2 1. Research Fellow, Department of Pharmacy, Karpagam University, Coimbatore. Tamil Nadu, India. 2 Department of Pharmacology, Karpagam University, Coimbatore,
More informationAntidiabetic Activity of Alcoholic Extract of Neem (Azadirachta Indica) Root Bark
RESEARCH ARTICLE Antidiabetic Activity of Alcoholic Extract of Neem (Azadirachta Indica) Root Bark Prabhakar Patil 1, Sudha Patil 2, Abhay Mane 3, Sushilkumar Verma 4 1 Department of Pharmacology, Navodaya
More informationANTIDIABETIC ACTIVITY OF KOVAI KIZHANGU CHOORANAM IN ALLOXAN INDUCED DIABETIC RATS ABSTRACT
ANTIDIABETIC ACTIVITY OF KOVAI KIZHANGU CHOORANAM IN ALLOXAN INDUCED DIABETIC RATS P. PARTHIBAN 1, RAVIKUMAR 1, J. ANBU *2 AND ASHWINI ANJANA 2 1 Department of Medicine, Govt. Siddha College, Arumbakkam,
More informationLAOYAN, EDEN K. APRIL Efficacy of Crude Extract of Resurrection Lily
BIBLIOGRAPHY LAOYAN, EDEN K. APRIL 2013. Efficacy of Crude Extract of Resurrection Lily (Kaempferia galanga Linn.) Against Striped Flea Beetle (Phyllotreta striloata Fab.) and Cabbage Butterfly (Peiris
More information