Brian C. Smith, Ph.D. Chief Technical Officer Big Sur Scientific (508)
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1 Measurement of Cannabinoid and Terpene Profiles in Cannabis Extracts by Mid-Infrared Spectroscopy Brian C. Smith, Ph.D. Chief Technical Officer Big Sur Scientific (508)
2 Outline I. Does the Cannabis Industry need In-House Testing? II. The BSS 2000 Cannabis Analyzer A. How it Works B. Establishing Spectrometer Street Cred C. Cannabinoids in Oils Results D. Terpenes in Oils Results E. Cannabinoids in Cannabis Buds and Solids Results III. Applications IV. Conclusions 2
3 The State of Cannabis Testing There are too frequent press reports of mis-labeling problems in the cannabis industry, due in part to Unrepresentative Sampling Cannabis instability Lack of standardized testing 3
4 Food Industry Testing Mill Grain Elevator Moo! = Where infrared analyzers determine price, quality, and process control Dairy = IR spectrometer testing 4
5 Cannabis Industry Testing Raw Material Where are the infrared analyzers to determine potency, quality, and process control? Dispensary Extractor Edibles Mfg. 5
6 The BSS 2000 Cannabis Analyzer Your Complete Cannabis Analysis System 6
7 How it Works I 1. Patent Pending United States non-provisional utility patent applied for 2. A general purpose quantitative mid-infrared spectrometer. 3. Mid-infrared is 10x-100x more sensitive and more selective than near infrared 7
8 How it Works II Ctrl+Click on the link below to view a video at our YouTube channel on how to use the BSS 2000 Cannabis Analyzer. BSS 2000 Cannabis Analyzer How to Use It Video Big Sur Scientific YouTube Channel 8
9 Experimental Mid-infrared spectra measured in triplicate using the BSS 2000 Cannabis Analyzer from Big Sur Scientific cm -1, where cannabinoids strongly absorb 12 cm -1 instrumental resolution MIR More sensitive and selective than NIR Analyzed 7 cannabis oil samples from Pop Naturals CO 2 oil not winterized but undergoes a proprietary process Cannabinoid and Terpene profiles measured by chromatography CW Analytical, Oakland CA Chemometric models built using infrared spectra and chromatographic data in Big Sur Scientific Model Builder Software PLS-1 algorithm Individual models built for each algorithm Generally 4-5 principal components used Savitsky-Golay 1 st derivative used in some cases 2 minutes/sample, push button operation Only need a few mgs of sample Calibrations customized to customers products and trusted lab All stated accuracies are averaged cross validation standard error of predictions 9
10 BSS 2000 Results 0.44 Spectrometer Street Cred - The Purell Test Test Quantitative abilities of the BSS % Ethanol label claim Diluted Purell sample volumetrically 7 standards measured in triplicate R 2 = Accuracy = ±1.2% Known volume % Ethanol 21 Data Points Wavenumbers [1/cm] 10
11 Spectra Stable Over Time 22 Spectra of same sample collected over 3 months 11
12 Oils Analysis - Cannabinoids BSS 2000 Results HPLC vs. BSS 2000 Results THCA in Oils 21 Data Points HPLC Results Cannabinoid Range in Wt. % R 2 Accuracy* THCA ±1.3 THC ±1.2 CBDA ±0.5 CBD ±0.2 CBGA ±0.1 CBG ±0.09 CBN ±0.03 CBC ±0.06 THCV ±0.01 *Average Cross Validation Standard Error of Prediction 12
13 Oils Analysis - Terpenes BSS 2000 Results HPLC vs. BSS 2000 Results β-caryophyllene in Oils 21 Data Points GC Results Analyte R 2 Accuracy, Wt. % Myrcene 0.99 ±0.05 Beta-Carophyllene 0.99 ±0.05 Limonene 0.99 ±0.05 Alpha-Pinene 0.93 ±0.06 Linalool 0.98 ±0.05 Alpha-Humulene 0.99 ±0.05 Nerolidol 0.99 ±0.05 Alpha-Terpinene 0.99 ±0.05 Gamma-Terpinene 0.99 ±0.05 Terpinolene 0.99 ±0.05 Camphene 0.99 ±
14 Cannabinoids in Bud BSS 2000 Results HPLC vs. IR Results, THCA in Buds HPLC vs. IR Results, 29 Data Points THCA in Buds 29 Data Points HPLC Results Analyte R 2 Accuracy, Wt. % THCA 0.98 ±1.5 THC 0.98 ±0.26 CBDA 0.98 ±0.9 CBD 0.98 ±0.1 CBN 0.98 ±
15 Cannabinoids in Cannabis Solids BSS 2000 Results Cannabis Solids = Shatter, resin, rosin, wax, & sugar HPLC vs. IR Results THCA in Shatter/Resin/Sugar/Wax 27 Data Points HPLC Results Analyte R 2 Accuracy, Wt. % THCA 0.99 ±1.9 THC 0.91 ±0.4 CBDA 0.99 ±0.62 CBD 0.94 ±0.1 CBN 0.8 ±0.1 15
16 BSS 2000 Applications Raw Material Dispensary Extractor Edibles Mfg. 16
17 Conclusions 1. There is a need in the Cannabis industry for more inhouse potency testing at more times and in more places than is currently available 2. The BSS 2000 accurately determines 9 cannabinoids and 11 terpenes in cannabis oils giving a cannabinoid and terpene profile 3. The BSS 2000 accurately determines THCA, THC, CBDA, CBD, and CBN in buds 4. The BSS 2000 accurately determines THCA, THC, CBDA, CBD, and CBN, and in shatter/resins/wax/sugar/rosin 5. Allows truly representative sampling 6. Allows cannabis businessed to perform potency testing in times and places previously thought impossible 17
18 Thank You! Big Sur Scientific (831)
Determination of Cannabinoid and Terpene Profiles in Cannabis Oils by Mid-Infrared Spectroscopy: 1. Cannabinoids
1 Brian C. Smith, Ph.D., Big Sur Scientific, brian@bigsurscientific.com, (508) 579-6514. Determination of Cannabinoid and Terpene Profiles in Cannabis Oils by Mid-Infrared Spectroscopy: 1. Cannabinoids
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