capacitation hyperactivation acrosome hyperactivation AR bovine serum albumin BSA non-genomic effect isothiocyanate; FITC PR mrna P hyperactivation HA
|
|
- Kathlyn McLaughlin
- 5 years ago
- Views:
Transcription
1 P PRP total RNA cdna PCR primer set PR mrna P hyperactivation HA AR Ca PR P HA AR P Ca PR mrna P DNA C PR PR P P HA AR Ca mrna capacitation hyperactivation acrosome reaction; AR hyperactivation AR Ca Ca ,2,6,7 Ca 1 bovine serum albumin BSA 8 non-genomic effect fluorescein isothiocyanate; FITC 9 Ca
2 48 PR PR PR hyperactivation AR Ca 1 HTF Sigma Chemical Co., Cat. No. 9962, Poole Dorset, UK Chirgwin et al. 10 total RNA 4 M 5.7 M Reverse transcription-polymerase chain reaction RT-PCR total RNA 200 ng random primer Takara, Kyoto, Japan RAV-2 reverse transcriptase cdna cdna template mm MgCl Taq DNA polymerase 94 C 1 55 C 1 72 C P15 forward P35 reverse 5 -AGGAGTTTGT- CAAGCTTCAA-3 5 -GGGACTGGATAAATG- TATTC-3 PR cdna 11 exon A B C mrna P36 forward P33 reverse 5 - CCCTCCGACGAAAAGACGC-3 5 -AAC- 1 isoform A, B, C
3 49 CTTGCACCCGGACCGGCTCA-3 DNA A/B A B mrna Southern blotting 5 µl P-labeled PR cdna probe WHO C 5 CO human serum albumin HSA; Sigma Chemical Co., St. Louis, MO, U.S.A. modified Biggers, Whitten and Whittinghams mbww 13 swim up C 5 CO 2 1 nm 10 nm 100 nm 1 µm mbww mbww 30 hyperactivated sperm Sigma Chemical Co. dimethylsulfoxide DMSO; Sigma Chemical Co. DMSO 1 mbww Hyperactivation Hamilton- Thorne Research Motility Analyzer HTM-IVOS, version 10, Beverly, MA, U.S.A. track velocity 100 microns/sec linearity 65 maximum amplitude for lateral displacement 7.5 microns hyperactivated sperm Burkman swim up mbww 6 10 nm 100 nm 500 nm 1 µm 3 µm 15 µm 30 µm mbww 30 acrosomereacted sperm Cross 16,17 Hoechst Sigma Chemical Co. 10 µg/ml fluorescein isothiocyanate-conjugated pisum sativum agglutinin FITC-PSA; Sigma Chemical Co. 30 Olympus BHT; Olympus, Tokyo Hoechst ARAcrosome cap FITC-PSA acrosome intact sperm equatrial segment acrosome-reacted sperm g 1 postestrous discharge pregnant mare s serum PMS; Sigma Chemical Co. 30 IU 3 human chorionic gonadotropin HCG; Mochida, Tokyo 30 IU HCG hyaluronidase Sigma Chemical Co. 15 mbww trypsin Sigma Chemical Co. mbww 3 mbww swim up
4 50 mbww 6 10 nm 100 nm 500 nm 1 µm 3 µm 15 µm 30 µm 1 ml mbww 30 mbww HSA mbww 300 µl Petri dish /ml 37 C 5 CO 2 4 Olympus BX, Olympus, Tokyo 5 Ca free mbww AR Ca 15 µm 5 Hyperactivation AR t χ2 1 A B C PR mrna exon P15/35 DNA A-B P36/ nm 2B A C mrna B A mrna A PR B A C mrna B PR B A mrna C β actin mrna Lane 1, ejaculated spermatozoa. Lane 2, proliferative phase uterine endometrium 200 ng. Lane 3, 50 ng. Lane 4, 13 ng. Lane 5, 3.1 ng. Lane 6, 0.78 ng. Lane 7, 0.20 ng. Lane 8, 0.05 ng. Lane 9, peripheral blood leucocyte. Lane 10, RT-PCR blank.
5 51 hyperactivated sperm p nm 3 µm acrosome-reacted sperm 15 µm 30 µm acrosome-reacted sperm p nm mm Ca 15 µm AR 3 Ca 15 µm acrosome-re- Progesterone 1 hyperactivation Progesterone n 8 Hyperactivated sperm mean SD 0nM nM nM nM * 1000nM ** **p 0.01 vs. 0nM 2 AR Acrosome-reacted sperm mean SD *p 0.05 vs. 0nM Hamster test of eggs penetrated 0nM nM nM nM * 1 µm ** 3 µm ** 15 µm ** 55.0** 30 µm ** 86.4** **p 0.01 vs. 0 µm 3 AR Ca *p 0.05 vs. 0 µm Ca 2+ Progesterone Acrosome-reacted sperm Hamster test mean SD of eggs penetrated 0mM 0 µm mM 15 µm * * 10.2 ** * * ** ** ** 1.71mM 0 µm mM 15 µm * 53.4 ** **p 0.01 *p 0.05
6 52 acted sperm p 0.05 p 0.01Ca-free Ca acrosome-reacted sperm p 0.05 p 0.01 hyperactivation AR 5 Ca 1,2,6,7 protein kinase C 19 PR PR 20 PR A B C 3 8 exon PR B mrna A mrna exon 1 C mrna exon 2 B A C PR mrna B A PR mrna RT-PCR mrna PR mrna mrna B A PR mrna DNA C PR PR mrna Uhler hyperactivation 10 nm hyperactivated sperm nm hyperactivated sperm hyperactivation Meizel AR 250 ng/ml 800 nm AR 22 Brucker 6 15 µm AR 2-3 µm Meizel AR AR µm
7 53 hyperactivation capacitation AR AR AR Hyperactivation AR Ca 1,2,6,7 Ca AR 25 Ca AR 26 Ca Ca Ca Ca channel blocker AR 27 Ca Ca PR Ca Ca Ca capacitation hyperactivation Ca ZP 3 Ca P Ca 1) Yanagimachi R: Mammalian fertilization. In: Knobil E, Neill JD, Eds. Physiology of Reproduction. New York; Ranvan Press, p , ) Blackmore PF et al: Progesterone and 17 alphahydroxyprogesterone. Novel stimulators of calcium influx in human sperm. J Biol Chem, 265: , ) Thomas P and Meizel S: An influx of extracellular calcium is required for initiation of the human sperm acrosome reaction induced by human follicular fluid. Gemete Res, 20: , ) Fabbri R et al: Follicular fluid and human granulosa cell cultures: influence on sperm kinetic parameters, hyperactivation, and acrosome reaction. Fertil Steril, 69: , ) Uhler ML: Direct effects of progesterone and antiprogesterone on human sperm hyperactivated motility and acrosome reaction. Fertil Steril, 58: , ) Brucker C et al: Progesterone-induced acrosome reaction: potential role for sperm acrosome reaction antigen-1 in fertilization. Hum Reprod, 9: , ) Fukui K et al: The effect of follicular fluid on intracellular free calcium levels in human spermatozoa. Asia-Oceania J Obstet Gynecol, 21: 43 49, ) Meizel S and Turner KO: Progesterone acts at the plasma membrane of human sperm. Mol Cell Endocrinol, 77: R1 R5, ) Tesarik J et al: Selective expression of a progesterone receptor on the human sperm surface. Fertil Steril, 58: , ) Chirgwin JM et al: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochem, 18: , ) Kastner P et al: Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B. EMBO J, 9: , ) World Health Organization: WHO laboratory manual for the examination of human semen and semen-cervical mucus interactions. Cambridge, 3 26, The Press Syndicate of Cambridge Univ, ) Biggers JD et al: Methods of mammalian embryology, Eds. Daniel JD, p , New York; Freeman & Co, ) Sperm preparation AIH 1: 39 45, ) Burkman LJ: Discrimination between nonhyperactivated and classical hyperactivated motility patterns in human spermatozoa using computerized analysis. Fertil Steril, 55: , ) Cross NL et al: Two simple methods for detecting acrosome-reacted human sperm. Gamete Res,
8 54 15: , ) Mendoza C et al: Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin. J Reprod Fertil, 95: , ) Yanagimachi R: The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod, 15: , ) Bonaccorsi L et al: Progesterone-stimulated intracellular calcium increase in human spermatozoa is protein kinase C-independent. Mol Hum Reprod, 4: , ) Mahesh VB et al: Diverse modes of action of progesterone and its metabolites. J Steroid Biochem Mol Biol, 56: , ) Uhler ML et al: Direct effects of progesterone and antiprogesterone on human sperm hyperactivated motility and acrosome reaction. Fertil Steril, 58: , ) Meizel S et al: Fertilization in Mammals, Eds. Bavister BD et al, p , Serono Symposia USA, Norwell, Massachusetts, ) Osman RA et al: Steroid induced exocytosis: the human sperm acrosome reaction. Biochem Biophys Res Commum, 160: , ) Cross NL et al: Induction of acrosome reaction by the human zona pellucida. Biol Reprod, 38: , ) Didion BA and Graves CN: Influence of calcium on the true acrosome reaction and capacity of bull spermatozoa to penetrate oocytes in vitro. J Dairy Sci, 72: , ) Effects of calcium and magnesium on in vitro fertilization in human 34: , ) Goodwin LO et al: Isolation and characterization of the primary structure of testis-specific L-type calcium channel: implications for contraception. Mol Hum Reprod, 3: , 1997.
Human Spermatozoa Attach to Trypsin-treated Hamster Zonae Pellucidae but do not Undergo Acrosome Reactions
Hiroshima J. Med. Sci. Vol.44, No.2, 47~51, June, 1995 HIJM 44-8 47 Human Spermatozoa Attach to Trypsin-treated Hamster Zonae Pellucidae but do not Undergo Acrosome Reactions Masatoshi KUMAGAI, Katsunori
More informationRole of a Capacitation-Related Protein on Some Sperm Functional Parameters
Role of a Capacitation-Related Protein on Some Sperm Functional Parameters ( boar sperm-zp interaction / monoclonal antibody / sperm antigens ) M. MOLLOVA 1, R. NEDKOVA 1, M. IVANOVA 1, T. DJARKOVA 1,
More informationγ-aminobutyric acid (GABA) induces the acrosome reaction in human spermatozoa
Molecular Human Reproduction vol.3 no.8 pp. 677 683, 1997 γ-aminobutyric acid (GABA) induces the acrosome reaction in human spermatozoa Qi-Xian Shi 1,3, Yu-Ying Yuan 1 and Eduardo R.S.Roldan 2 1 Department
More informationThe effect of albumi~ gradients and human serum on the longevity and fertilizing capacity of human spermatozoa in the hamster ova penetration assay*
FERTn.1TY AND STERIL1TY Copyright c 1982 The American Fertility Society Vol. 38, No.2, August 1982 Printed in U.SA. The effect of albumi~ gradients and human serum on the longevity and fertilizing capacity
More informationEvaluation of the Predictive Value of Semen Parameters in Sperm Fertility Potential Using Intracellular Calcium Increase in Response to Progesterone
Iranian Journal of Reproductive Medicine Vol.1, No.1 pp. 24-28, 23. Evaluation of the Predictive Value of Semen Parameters in Sperm Fertility Potential Using Intracellular Calcium Increase in Response
More informationCapacitated sperm cells react with different types of antisperm antibodies than fresh ejaculated sperm*
FERTILITY AND STERILITY Copyright Q 1992 The American Fertility Society Vol. 57, No.2, February 1992 Printed on acid-free paper in U.S.A. Capacitated sperm cells react with different types of antisperm
More informationSerum hormone levels affect sperm function*
FERTILITY AND STERILITY Copyright 1990 The American Fertility Society Vol. 54. No.1. July 1990 Printed on acid-free paper in U.S.A. Serum hormone levels affect sperm function* Mike T. Mbizvo, D.Phil.t
More informationDe Yi Liu, Ph.D.t Harold Bourne, B.Sc. H. W. Gordon Baker, M.D., Ph.D.
FERTILITY AND STERILITY Vol. 64, No.1, July 1995 Copyright i) 1995 American Society for Reproductive Medicine Printed on acid-free paper in U. S. A. i' I Fertilization and pregnancy with acrosome intact
More informationHuman sperm penetration assay as an indicator of sperm function in human in vitro fertilization
FERTILITY AND STERILITY Copyright., 1987 The American Fertility Society Vol. 48, No. 2, August 1987 Printed in U.S.A. Human sperm penetration assay as an indicator of sperm function in human in vitro fertilization
More informationProteasome activity and its relationship with protein phosphorylation during capacitation and acrosome reaction in human spermatozoa
Proteasome activity and its relationship with protein phosphorylation Proteasome activity and its relationship with protein phosphorylation during capacitation and acrosome reaction in human spermatozoa
More informationSandro C. Esteves, M.D. Rakesh K. Sharma, Ph.D. Anthony J. Thomas, Jr., M.D. Ashok Agarwal, Ph.D.
Int J Fertil, 43 (5), 1998 p. 235-242 1998 U.S. International Foundation for Studies in Reproduction, Inc., the Falloppius International Society, the International Society of Reproductive Medicine, the
More informationInduction of the human sperm acrosome reaction by human oocytes*
FERTILITY AND STERILITY Copyright C> 1988 The American Fertility Society Vol. 50, No.6, December 1988 Printed in U.S.A. Induction of the human sperm acrosome reaction by human oocytes* Christopher J. De
More informationThe Use of Zona-Free Animal Ova as a Test-System for the
BIOLOGY OF REPRODUCTION 15, 471-476 (1976) The Use of Zona-Free Animal Ova as a Test-System for the Assessment of the Fertilizing Capacity of Human Spermatozoa R. YANAGIMACHI, H. YANAGIMACHI and B. J.
More informationEffects of phosphodiesterase inhibitors caffeine and pentoxifylline on spontaneous and stimulus-induced acrosome reactions in human sperm
FERTILITY AND STERILITY Copyright CI 1992 The American Fertility Society Vol. 58, No.6, December 1992 Printed on acid-free paper in U.S.A. Effects of phosphodiesterase inhibitors caffeine and pentoxifylline
More informationAssessment of released acrosin activity as a measurement of the sperm acrosome reaction
DOI: 10.1111/j.1745-7262.2008.00312.x www.asiaandro.com. Original Article. Assessment of released acrosin activity as a measurement of the sperm acrosome reaction Rui-Zhi Liu 1, Wan-Li Na 2, Hong-Guo Zhang
More informationIn-vitro fertilization in the mouse and the relevance of different sperm/egg concentrations and volumes
In-vitro fertilization in the mouse and the relevance of different sperm/egg concentrations and volumes A. K. S. Siddiquey and J. Cohen Department ofobstetrics and Gynaecology, Birmingham Maternity Hospital,
More informationAldo E. Calogero, M.D.,* Nunziatina Burrello, Ph.D.,* Emanuela Ferrara, M.D.,* Jenny Hall, Ph.D., Simon Fishel, Ph.D., and Rosario D Agata, M.D.
FERTILITY AND STERILITY VOL. 71, NO. 5, MAY 1999 Copyright 1999 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. -Aminobutyric acid (GABA)
More informationFertilization depends on mechanisms that help sperm meet eggs of the same species.
Fertilization depends on mechanisms that help sperm meet eggs of the same species. www.uchsc.edu/ltc/fertilization.html Fertilization union of sperm and egg Is a chain of events. Interruption of any step
More informationDetection of Wild Type and Variant mrna Transcript of Progesterone Receptor in Human Spermatozoa of Infertile Males
International Journal of Research Studies in Microbiology and Biotechnology (IJRSMB) Volume 2, Issue 1, 2016, PP 28-32 ISSN 2454-9428 (Online) www.arcjournals.org Detection of Wild Type and Variant mrna
More informationHigh potassium concentration and the cumulus corona oocyte complex stimulate the fertilizing capacity of human spermatozoa *
FERTILITY AND STERILITY Copyright'" 1990 The American Fertility Society Printed on acid-free paper in U.S.A. High potassium concentration and the cumulus corona oocyte complex stimulate the fertilizing
More informationSemen was collected by masturbation from 33 infertile patients undergoing diagnostic evaluation MATERIALS AND METHODS
FERTILITY AND STERILITY Copyright Clt989 The American Fertility Society Printed in U.S.A. Variation of movement characteristics with washing and capacitation of spermatozoa. I. Univariate statistical analysis
More informationIN VITRO FERTILIZATION OF RABBIT EGGS IN OVIDUCT SECRETIONS FROM DIFFERENT DAYS BEFORE AND AFTER OVULATION*
FERTILITY AND STERILITY Copyright~ 1975 The American Fertility Society Vol. 26, No.7, July 1975 Printed in U.SA. IN VITRO FERTILIZATION OF RABBIT EGGS IN OVIDUCT SECRETIONS FROM DIFFERENT DAYS BEFORE AND
More informationhamster ova A chromosomal method to distinguish between X- and Y-bearing spermatozoa of the bull in zona-free offspring.
A chromosomal method to distinguish between X- and Y-bearing spermatozoa of the bull in zona-free hamster ova H. Tateno and K. Mikamo Department of Biological Sciences, Asahikawa Medical College, Asahikawa,
More informationWAVE DYNAMYCS OF HUMAN SPERM MOTILITY
Page 1 of 14 WAVE PARAMETERS OF THE SPERM FLAGELLUM AS PREDICTORS OF HUMAN SPERMATOZOA MOTILITY Andrologia 30: 153-157, 1998 O. Vera 1, M.G. Muñoz 2 and K. Jaffe 2 1 Facultad de Ciencias Veterinarias,
More informationMolecular BASIS OF FERTILIZATION
COLLEGE OF HEALTH SCIENCE DEPARTMENT OF PHYSIOLOGY PRESENTATION ON: Molecular BASIS OF FERTILIZATION By TEKETEL ERISTU Kediso 1 Presentation Outline Introduction Fertilization Types of Fertilization Cellular
More informationEffects of Microinjection of Glutathione on Male Pronucleus Formation in Porcine Oocytes Matured in Vitro
Journal of Reproduction and Development, Vol. 38, No. 2, 1992 Effects of Microinjection of Glutathione on Male Pronucleus Formation in Porcine Oocytes Matured in Vitro Kunihiko NAITO and Yutaka TOYODA
More informationEffect of Swim-up Sperm Washing and Subsequent Capacitation on Acrosome Status and Functional Membrane Integrity of Normal Sperm
Int / Fertil.45 (5), 2000 p. 335-341 2000 U.S. International Foundation for Studies in Reproduction. Inc., the Falloppius International Society, the International Society of Reproductive Medicine, the
More informationChanges in Motility Parameters of Mouse Spermatozoa in Response to Different Doses of Progesterone during Course of Hyperactivation
Changes in Motility Parameters of Mouse Spermatozoa in Response to Different Doses of Progesterone during Course of Hyperactivation Majid Katebi *1, Mansoureh Movahedin 2, Mir Abbas Abdolvahabi 1, Mohammad
More informationEffects of human follicular fluid on spermatozoa that have been cocultured with human oviductal cells
FERTILITY AND STERILITY VOL. 72, NO. 6, DECEMBER 1999 Copyright 1999 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. Effects of human
More informationSpermac stain analysis of human sperm acrosomes
FERTILITY AND STERILITY VOL. 72, NO. 1, JULY 1999 Copyright 1999 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. Spermac stain analysis
More informationCharacterization of Anti-Hamster ZP-0 Monoclonal Antibody
Characterization of Anti-Hamster ZP-0 Monoclonal Antibody K. Ookata (1), K.Takagishi (1), S. Konno (2) and T. Oikawa(1,2) (1) Developmental and Reproductive Biology Center, Yamagata 990, Japan and (2)
More informationISOQUERCITRIN ON THE BEHAVIOR OF
BENEFICIAL EFFECTS OF ISOQUERCITRIN ON THE BEHAVIOR OF BOVINE SPERMATOZOA IN VITRO Hana Greifová*, Eva Tvrdá, Norbert Lukáč 8th CASEE Conference "Sustainable development in Europe cooperation between science
More informationHuman follicular fluid stimulates hyperactivated motility in human sperm*t
FERTILITY AND STERILITY Copyright 1990 The American Fertility Society Printed on acid-free paper in U.S.A. Human follicular fluid stimulates hyperactivated motility in human sperm*t Michael T. Mbizvo,
More informationHyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm
Human Reproduction Vol.22, No.10 pp. 2632 2638, 2007 Advance Access publication on July 26, 2007 doi:10.1093/humrep/dem245 Hyperactivation of capacitated human sperm correlates with the zona pellucida-induced
More informationTable S1. Primers and PCR protocols for mutation screening of MN1, NF2, KREMEN1 and ZNRF3.
Table S1. Primers and PCR protocols for mutation screening of MN1, NF2, KREMEN1 and ZNRF3. MN1 (Accession No. NM_002430) MN1-1514F 5 -GGCTGTCATGCCCTATTGAT Exon 1 MN1-1882R 5 -CTGGTGGGGATGATGACTTC Exon
More informationRelationship between human in-vitro fertilization and intracytoplasmic sperm injection and the zona-free hamster egg penetration test
Human Reproduction vol.13 no.7 pp.1928 1932, 1998 Relationship between human in-vitro fertilization and intracytoplasmic sperm injection and the zona-free hamster egg penetration test Hiroaki Shibahara,
More informationANDROLOGY. Effects of Hydrogen Peroxide on Human Spermatozoa* ANDROLOGY. SERGIO OEHNINGER, 1'3 PETER B LACKMORE, ~ MARY MAHONY, ~ and GARY HODGEN 1
ANDROLOGY ANDROLOGY Effects of Hydrogen Peroxide on Human Spermatozoa* SERGIO OEHNINGER, 1'3 PETER B LACKMORE, ~ MARY MAHONY, ~ and GARY HODGEN 1 Submitted: December 15, 1994 Accepted: January 4, 1995
More informationREPRODUCTION PERFORMANCE OF RABBITS BY INCUBATED SEMEN WITH HEPARIN IN INDUSTRY RABBIT FARM
Lucrări ştiinţifice Zootehnie şi Biotehnologii, vol. 42 (1) (2009), Timişoara REPRODUCTION PERFORMANCE OF RABBITS BY INCUBATED SEMEN WITH HEPARIN IN INDUSTRY RABBIT FARM PERFORMANTELE REPRODUCTIVE OBŢINUTE
More information: O (streptolysin O, SLO) : 10~12 ICR,, /ml, U/ml SLO 15 min, SLO, (PI) : (P>0.05),,,,, (P<0.05) : SLO,, : O(SLO); ; (PI) ; (IVF)
34 11 Vol.34 No.11 2014 11 Nov. 2014 Reproduction & Contraception doi: 10.7669/j.issn.0253-357X.2014.11.0887 E-mail: randc_journal@163.com O 1 12 12 1 1 3 (1. 200032) (2. 200011) (3. 200011) : O (streptolysin
More informationSperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T -6 antibody*
FERTILITY AND STERILITY Copyright 199 The American Fertility Society Printed on acid-free paper in U.S.A. Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with
More informationComplement-mediated effects of sperm head-directed human antibodies on the ability of human spermatozoa to penetrate zona-free hamster eggs
FERTILITY AND STERILITY Copyright " 1983 The American Fertility Society Printed in U.BA. Complement-mediated effects of sperm head-directed human antibodies on the ability of human spermatozoa to penetrate
More informationHow does a sperm fertilise a human egg in vitro?
How does a sperm fertilise a human egg in vitro? A. Henry Sathananthan & Christopher Chen Monash University, Melbourne & Flinders Medical Centre, S. Australia & Singapore Sperm-oocyte fusion is the key
More informationThe expression and significance of CATSPER1 in human testis and ejaculated spermatozoa
Asian J Androl 2006; 8 (3): 301 306 DOI: 10.1111/j.1745-7262.2006.00132.x. Original Article. The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa Hong-Gang Li, Ai-Hua
More informationAcrobeads test: a new diagnostic test for assessment of the fertilizing capacity of human spermatozoa*
FERTILITY AND STERILITY Copyright 1995 American Society for Reproductive Medicine Vol. 63, No.3, March 1995 Printed on acid-free paper in U. S. A. Acrobeads test: a new diagnostic test for assessment of
More informationInhibition of the acrosome reaction by trypsin inhibitors and prevention of penetration of spermatozoa through the human zona pellucida
Inhibition of the acrosome reaction by trypsin inhibitors and prevention of penetration of spermatozoa through the human zona pellucida M. Llanos1, P. Vigil2, A. M. Salgado2 and P. Morales2 'L/m'f of Reproductive
More informationSperm, Progesterone, and CatSper Ion Channel. Polina V. Lishko, Ph.D. University of California, Berkeley
Sperm, Progesterone, and CatSper Ion Channel Polina V. Lishko, Ph.D. University of California, Berkeley Human contraception: 1. Arrest of spermato- and/or spermiogenesis or inhibition of the oocyte maturation.
More informationSummary. Mouse eggs were fertilized in vitro, in the presence and
THE R\l=O^\LEOF CUMULUS CELLS AND THE ZONA PELLUCIDA IN FERTILIZATION OF MOUSE EGGS IN VITRO A. PAVLOK and ANNE McLAREN Czechoslovak Academy of Sciences, Laboratory of Animal Genetics, Libechov, Czechoslovakia,
More informationComputer-aided evaluation of assessment of grade a spermatozoa by experienced technicians
Computer-aided evaluation of assessment of grade a spermatozoa by experienced technicians Trevor G. Cooper, Ph.D., and Ching-Hei Yeung, Ph.D. Institute of Reproductive Medicine of the University of Münster,
More informationEffects of Cryopreservation on the Ultrastructure of Human Testicular Sperm
Journal of Reproduction & Contraception (2005) 16 (4):195-200 ORIGINAL PAPER Effects of Cryopreservation on the Ultrastructure of Human Testicular Sperm Xin-qiang LAI 1, Wei-jie ZHU 2, Jing LI 3, Fu-xing
More informationPENETRATION OF THE ZONA FREE HAMSTER EGG BY HUMAN SPERM*
FERTILITY AND STERILITY Copyright t, 1980 The American Fertility Society Vol, 33, No, 3, March 1980 Printed in U,SA, PENETRATION OF THE ZONA FREE HAMSTER EGG BY HUMAN SPERM* ZVI BINOR, M.D. JOSEPH E. SOKOLOSKI,
More informationAlbumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro
Albumin is required to support the acrosome reaction but not capacitation in mouse spermatozoa in vitro Lynn R. Fraser Department of Anatomy and Human Biology, King's College, Strand, London WC2R 2LS,
More informationMicroinsemination (Intracytoplasmic Sperm Injection) Microinsemination schedule. 1. Preparation of mediums
Microinsemination (Intracytoplasmic Sperm Injection) Masumi Hirabayashi Section of Mammalian Transgenesis, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National
More informationDefective sperm zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization
Human Reproduction vol.15 no.3 pp.702 708, 2000 Defective sperm zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization D.Y.Liu 1 and H.W.G.Baker However,
More informationPENETRATION OF HUMAN SPERMATOZOA INTO THE HUMAN ZONA PELLUCIDA AND THE ZONA-FREE HAMSTER EGG: A STUDY OF FERTILE DONORS AND INFERTILE PATIENTS*
FERTILITY AND STERILiTY Copyright" 1980 The American Fertility Society ""I. 33, No.5, May 1980 Prinred in U.SA. PENETRATION OF HUMAN SPERMATOOA INTO THE HUMAN ONA PELLUCIDA AND THE ONA-FREE HAMSTER EGG:
More informationApproximately 8 min were required for the cortical granule material
THE ZONA REACTION OF HAMSTER AND MOUSE EGGS: PRODUCTION IN VITRO BY A TRYPSIN-LIKE PROTEASE FROM CORTICAL GRANULES R. B. L. GWATKIN, D. T. WILLIAMS, J. F. HARTMANN and M. KNIAZUK Merck Institute for Therapeutic
More informationEnvironment and medium volume influence in vitro fertilisation of pig oocytes
Zygote 1 (August), pp 209-213. Copyright 1993 Cambridge University Press Printed in Great Britain Environment and medium volume influence in vitro fertilisation of pig P. Coy 1, E. Martinez 2, S. Ruiz
More informationDepartment of Obstetrics and Gynaecology, University of Melbourne, Reproductive Biology Unit, Royal Women's Hospital, Melbourne, Victoria, Australia
FERTILITY AND STERILITY Copyright c 1988 The American Fertility Society Vol. 50, No. 2, August 1988 Printed in U.S.A. The proportion of human sperm with poor morphology but normal intact acrosomes detected
More informationInternal Fertilization
Internal Fertilization Fertilization which takes place inside the female body is called internal fertilization(the union of the gametes within the female body after insemination) Occurs in the widest part
More informationThe Time of Cortical Granule Breakdown and Sperm Penetration in Mouse and Hamster Eggs Inseminated in vitro
BIOLOGY OF REPRODUTION 19, 261-266 (1978) The Time of ortical Granule Breakdown and Sperm Penetration in Mouse and Hamster Eggs Inseminated in vitro Y. FUKUDA1 and M.. HANG2 Worcester Foundation for Experimental
More informationK. Bedu-Addo 2 and S.I. Publichover 2 Department of Physiology, School of Medical sciences, 1 College of Health Sciences,
POTASSIUM IONS IMPROVE THE PROGESTERONE-INDUCED [Ca 2+ ] i INCREASE AND ACROSOME REACTION AS WELL AS THE POTAS- SIUM-INDUCED [Ca 2+ ] i INCREASE IN HUMAN SPERMATOZOA K. Bedu-Addo 2 and S.I. Publichover
More informationStudy Guide Answer Key Reproductive System
Biology 12 Human Biology Textbook: BC Biology 12 Study Guide Answer Key Reproductive System 1. Distinguish between a gamete and a gonad using specific examples from the male and female systems. Gonads
More informationAssessment of human sperm acrosome reaction by flow cytometry: validation and evaluation of the method by fluorescence-activated cell sorting*
FERTILITY AND STERILITY Vol. 60. No.6. December 1993 Copyright 1993 The American Fertility Society Printed on acid-free paper in U. s. A. Assessment of human sperm acrosome reaction by flow cytometry:
More informationEffect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes
Theriogenology Insight: 4(3): 17-111, December, 214 DOI Number: 1.98/2277-3371.214.74.2 Effect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes K M
More informationSperm production. Sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Sperm production Ductus deferens Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationSperm production. Sperm production. Controlling sperm production. Meiosis. Mitosis. The cells of Leydig in testes secrete
Ductus deferens Sperm production Epididymis The cells of Leydig in testes secrete Seminiferous testosterone (T) tubules T secreted at puberty produces 2 o sex characteristics, spermatogenesis, & maintain
More informationWhat functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs?*t
,. I FERTILITY AND STERILITY Copyright ~ 1983 The American Fertility Society Vol. 40, No.3, September 1983 Printed in U.SA. What functions of the sperm cell are measured by in vitro fertilization of zona-free
More informationEffect of Iysoplatelet-activating factor on human sperm fertilizing ability*
FERTILITY AND STERILITY Vol. 59, No.4, April 1993 Copyright!l'J 1993 The American Fertility Society Printed on acid-free paper in U.S.A. Effect of Iysoplatelet-activating factor on human sperm fertilizing
More informationDevelopmental Biology Biology Fertilization. October 19, 2006
Developmental Biology Biology 4361 Fertilization October 19, 2006 Fertilization Fertilization accomplishes two things: Sex (combining genes from two genomes) Reproduction (initiates reactions in the egg
More informationDegree of Cortical Granule Exocytosis in in vitro- matured Porcine Oocytes Induced by. Different Artificial Stimulators
Advanced Studies in Biology, Vol. 3, 2011, no. 7, 297-307 Degree of Cortical Granule Exocytosis in in vitro- matured Porcine Oocytes Induced by Different Artificial Stimulators Samur Thanoi 1*, Chainarong
More informationHuman sperm hyperactivation in whole semen and its association with low superoxide scavenging capacity in seminal plasma*
FERTILITY AND STERILITY Copyright
More informationPrince of Wales Hospital, Shatin, New Territories, Hong Kong, China
REPRODUCTIVE BIOLOGY FERTILITY AND STERILITY VOL. 75, NO. 5, MAY 2001 Copyright 2001 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. Regulation
More informationSuccessful fertilization in vitro of fresh intact oocytes by perivitelline (acrosome-reacted) spermatozoa of the rabbit*
FERTILITY AND STERILITY Copyright 1984 The American Fertility Society Vol. 41, 5, May 1984 Printed in U.8A. Successful fertilization in vitro of fresh intact oocytes by perivitelline (acrosome-reacted)
More informationChanges in human sperm motion during capacitation
Changes in human sperm motion during capacitation in vitro P. Morales, J. W. Overstreet, and D. F. Katz Department of Obstetrics and Gynecology, Division of Reproductive Biology and Medicine, University
More informationH.Van de Velde 1, Z.P.Nagy, H.Joris, A.De Vos and A.C.Van Steirteghem
Human Reproduction vol.12 no.10 pp.2246 2250, 1997 Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection
More informationAn evaluation of various treatments to increase sperm penetration capacity for potential use in an in vitro fertilization program
FERTILITY AND STERILITY Copyright c 1992 The American Fertility Society Printed on ocid-free paper in U.S.A. An evaluation of various treatments to increase sperm penetration capacity for potential use
More informationDerived copy of Fertilization *
OpenStax-CNX module: m56433 1 Derived copy of Fertilization * Stephanie Fretham Based on Fertilization by OpenStax This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution
More informationA novel view of albumin-supported sperm capacitation: role of Lipid Transfer Protein-I*t*
FERTILITY AND STERILITY Copyright e 1993 The American Fertility Society Vol. 59, No.3, March 1993 Printed on acid-free paper in U.S.A. A novel view of albumin-supported sperm capacitation: role of Lipid
More informationAbsence of glucose decreases human fertilization and sperm movement characteristics in vitro
hrep$$0107 Human Reproduction vol.12 no.1 pp.119 123, 1997 Absence of glucose decreases human fertilization and sperm movement characteristics in vitro Maha M.Mahadevan 1, Michael M.Miller and (Edwards,
More informationA human sperm antigen possibly involved in binding and/ or fusion with zona-free hamster eggs
FERTILITY AND STERILITY Copyright 1990 The American Fertility Society Vol. 54, No.6, December 1990 Printed on ocid-free paper in U.S.A. A human sperm antigen possibly involved in binding and/ or fusion
More informationScanning Electron Microscopic Observations on the Sperm Penetration through the Zona Pellucida of Mouse Oocytes Fertilized in vitro
Scanning Electron Microscopic Observations on the Sperm Penetration through the Zona Pellucida of Mouse Oocytes Fertilized in vitro Masatsugu MOTOMURA and Yutaka TOYODA School of Veterinary Medicine and
More informationSpermatogenesis. What is it and what does it look like? How do hormones regulate spermatogenesis?
Spermatogenesis What is it and what does it look like? How do hormones regulate spermatogenesis? FSH, androgens, growth factors Animal Physiology (Hill, Wise, Anderson): Ch. 15 435-438 1 Spermatogenesis:
More informationDevelopment of a predictive model for optimal zona pellucida binding using insemination volume and sperm concentration*
FERTILITY AND STERILITY Copyright c 1994 The American Fertility Society Vol. 62, No. 4, October 1994 Printed on acid-free paper in U. S. A. Development of a predictive model for optimal zona pellucida
More informationEffect of glycerol on the penetrating ability of fresh ram spermatozoa with zona-free hamster eggs
Effect of glycerol on the penetrating ability of fresh ram spermatozoa with zona-free hamster eggs T. Slav\l=i'\k The Czechoslovak Academy of Sciences, Institute of Animal Physiology and Genetics, CS-277
More informationSperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes
Journal of Reproduction and Development, Vol. 46, No. 5, 2000 Original Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes Hiroaki FUNAHASHI
More informationReproductive Endocrinology. Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007
Reproductive Endocrinology Isabel Hwang Department of Physiology Faculty of Medicine University of Hong Kong Hong Kong May2007 isabelss@hkucc.hku.hk A 3-hormone chain of command controls reproduction with
More informationInfluence of sperm immobilization on onset of Ca 2 oscillations after ICSI
Human Reproduction Vol.16, No.1 pp. 148 152, 2001 Influence of sperm immobilization on onset of Ca 2 oscillations after ICSI K.Yanagida 1,3, H.Katayose 1, S.Hirata 2, H.Yazawa 1, S.Hayashi 1 and A.Sato
More informationCorrelation of human in vitro fertilization with the hamster egg bioassay*
F'ERTIlJTY AND STERILITY Copyright ~ 1983 The American Fertility Society Vol. 40, No. I, July 1983 Printed in U.8A. Correlation of human in vitro fertilization with the hamster egg bioassay* Don P. Wolf,
More informationMount Sinai School of Medicine, New York, and Albert Einstein College of Medicine, Bronx, New York
FERTILITY AND STERILITY Copyright ro 1992 The American Fertility Society Printed on acid-free paper in U.S.A. The fertilization antigen-l does not have proteolytic/ acrosin activity, but its monoclonal
More informationSperm nuclear chromatin normality: relationship with sperm morphology, sperm-zona pellucida binding, and fertilization rates in vitro*
FERTILITY AND STERILITY Copyright Q 1992 The American Fertility Society Vol. 58, No.6, December 1992 Printed on acid-free paper in U.S.A. Sperm nuclear chromatin normality: relationship with sperm morphology,
More informationEvaluation for antisperm antibodies after storage of sperm TEST-yolk buffer*
,---- FERTILITY AND STERILITY Vol. 66, No.3, September 1996 Copyright 'l" 1996 American Society for Reproductive Medicine Evaluation for antisperm antibodies after storage of sperm TEST-yolk buffer* Printed
More informationAnimal Reproduction. Spermatozoa in female tract. # lectures for cumulative test # 03 book
Animal Reproduction JP Advis DVM, Ph.D. Bartlett Hall, Animal Sciences, Cook, (732) 932-9240, advis@aesop.rutgers.edu 21 Course website: rci.rutgers.edu/~advis Material to be covered: About lecture Meetings
More informationChapter 4. Estrogen receptor expression in human macrophages
Chapter 4 Estrogen receptor expression in human macrophages 4.1. Introduction Macrophages respond to estrogen present in their microenvironment and hence should express functional estrogen receptors unless
More informationTitle. Author(s)TEKELI, Tevfik; KWEON, Oh Kyeong; KANAGAWA, Hiroshi. CitationJapanese Journal of Veterinary Research, 35(4): 283-
Title THE VIABILITY OF DEEP-FROZEN AGGREGATED MOUSE EMBRYO Author(s)TEKELI, Tevfik; KWEON, Oh Kyeong; KANAGAWA, Hiroshi CitationJapanese Journal of Veterinary Research, 35(4): 283- Issue Date 1987-10-30
More informationAcupuncture Treatment For Infertile Women Undergoing Intracytoplasmic Sperm injection
Acupuncture Treatment For Infertile Women Undergoing Intracytoplasmic Sperm injection Sandra L. Emmons, MD Phillip Patton, MD Source: Medical Acupuncture, A Journal For Physicians By Physicians Spring
More informationAnti-Spermatozoa Antibody ELISA for use with seminal plasma
Instructions for use Anti-Spermatozoa Antibody ELISA for use with seminal plasma Cat. No.: BS-10-21 Size: 12 strips with 8 wells each (individually breakable) Storage: 2 C 8 C (36 F 46 F) Enzyme linked
More informationABSTRACT. Key words: ovulation, ovary, human, follicle, collagen, MMP and TIMP. ISBN-10: ISBN-13:
HUMAN OVULATION Studies on collagens, gelatinases and tissue inhibitors of metalloproteinases Anna Karin Lind Department of Obstetrics and Gynecology Institute of Clinical Sciences Sahlgrenska University
More informationEndogenous folic acid is essential for normal development of preimplantation embryos
Human Reproduction vol.13 no.5 pp.1312 1316, 1998 Endogenous folic acid is essential for normal development of preimplantation embryos C.O Neill Human Reproduction Unit, Department of Physiology, University
More informationSperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin
Reproductive BioMedicine Online (2010) 20, 132 137 www.sciencedirect.com www.rbmonline.com ARTICLE Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of
More informationREPRODUCTIVE BIOLOGY. Calcium requirements for human sperm function in vitro
REPRODUCTIVE BIOLOGY FERTILITY AND STERILITY VOL. 79, NO. 6, JUNE 2003 Copyright 2003 American Society for Reproductive Medicine Published by Elsevier Inc. Printed on acid-free paper in U.S.A. Calcium
More informationBiology 4361 Developmental Biology. October 11, Multiple choice (one point each)
Biology 4361 Developmental Biology Exam 1 October 11, 2005 Name: ID#: Multiple choice (one point each) 1. Sertoli cells a. surround spermatocytes b. are the structural components of the seminiferous tubules
More information