OOSIGHT IVF. See what you ve been missing.
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1 OOSIGHT IVF IMAGING SYSTEM See what you ve been missing.
2 Oosight. The non-invasive imaging system that reveals critical structures in the oocyte to enable better grading. Adding Oosight to your lab can improve success by offering you a quantitative and reproducible method to measure biological disruption in either fresh or previously frozen Disrupted Spindle - Tripolar oocytes. You can now select against oocytes for ICSI and embryos for implantation. Understanding the oocyte is critical to understanding the foundation of embryogenesis. Studies show that a disrupted spindle apparatus or a weakened zona pellucida in the oocyte have yielded lower pregnancy rates. In fact, pregnancy is 8-times more likely when the inner zona pellucida is well-ordered, so don t be fooled into thinking that Inner Zona Retardance = 2.53 nm oocyte assessment is a waste of time. 1 1 Shen Y, et al. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Human Reproduction, 2005 Jun;20(6): hotography credits Front cover Retardance pseudocolor image of MII human oocyte Back cover Retardance grayscale image of MII human oo
3 Oocyte screening can make all the difference. Oosight enables you to determine which subpopulation of oocytes are at high risk for producing chromosomally abnormal embryos. One in approximately 20 cycles contains oocytes that are immature but are nevertheless falsely labeled MII using conventional imaging techniques. Oosight can prevent the potentially damaging effects that result from injecting immature oocytes. The system can also help screen for oocytes with highly disrupted spindles, such as those that are multipolar. It s an easy add-on. The Oosight system integrates seamlessly onto your ICSI workstation. Now with foot-pedal controls and automated spindle and zona tools, measuring each oocyte is a simple 2-step process. Snap an image and then press a button to report the data. Meaningful retardance and orientation data are organized into an exportable worksheet. It s really that simple. cyte Before/After image above spec chart courtesy of RMA NJ before From the conventional constrast before image, structures such as the spindle and multiple layers of the zona pellucida remain invisible. Images to the right display the various analysis tools in the Oosight software. Orientation Color Map Orientation Vector Map Retardance Color Map Automated Spindle Finder TM Automated Inner-zona Finder TM
4 Oocyte screening can make all the difference. Oosight enables you to determine which subpopulation of oocytes are at high risk for producing chromosomally abnormal embryos. One in approximately 20 cycles contains oocytes that are immature but are nevertheless falsely labeled MII using conventional imaging techniques. Oosight can prevent the potentially damaging effects that result from injecting immature oocytes. The system can also help screen for oocytes with highly disrupted spindles, such as those that are multipolar. after Oosight outperforms other systems. Even if you use other contrastenhancement methods, none of them delivers the performance of Oosight when it comes to highcontrast quantitative imaging. With its unprecedented resolution and calibrated setup, Oosight provides the reproducibility and sensitivity required of a grading routine. Improve the efficiency of oocyte cryopreservation. It s an easy add-on. The Oosight system integrates seamlessly onto your ICSI workstation. Now with foot-pedal controls and automated spindle and zona tools, measuring each oocyte is a simple 2-step process. Snap an image and then press a button to report the data. Meaningful retardance and orientation data are organized into an exportable worksheet. It s really that simple. Oosight image of human MII oocyte taken just prior to ICSI. The spindle apparatus is nicely barrel shaped and the three layers of the zona pellucida are all visible. Whenever a clinical application is known to negatively alter the state of the biological material being used, it is imperative that checks and balances are in place to monitor the extent of that change. Oosight can help do this for all cryopreserved oocytes by providing a method that helps to ensure that vital structures in the oocyte are reformed to their original, pre-frozen state. Contact us at or at to learn more about Oosight or to schedule a demonstration of this breakthrough system.
5 strong weak The Measure of Birefringence On the left, human MII oocyte has a strongly birefringent, barrel-shaped spindle (2.5nm mean retardance) and a well ordered inner zona (average max retardance = 2.7nm). On the right, this human MII oocyte has a weakly birefringent spindle (1.2nm mean retardance), and a highly disrupted inner zona as evidenced by the vector map and low average max retardance (1.7nm). Oosight IVF Microscope specific optical components* 1.3 Megapixel CCD Camera Image Capture Software Automated Analysis Tools Movie Capture Basic Meta *The Oosight system is compatible with most research-grade microscopes and third party laser systems. Glass bottom dishes are required. Non-invasive oocyte spindle imaging is advancing the science of oocyte cryopreservation. The use of the Oosight Imaging System should help to better select embryos for transfer. Dr. N Noyes, NYU IVF rogram
6 About CRi Cambridge Research & Instrumentation, Inc (CRi) is a Boston-based biomedical imaging company providing innovative optical imaging solutions to our customers for more than 20 years. Our multidisciplinary team is dedicated to working with our academic and commercial customers to provide high-value solutions. We provide comprehensive imaging and analysis solutions that enable the user to investigate and characterize biological phenotypes while preserving spatial context. With over 80 patents pending and issued, CRi s solution platforms encompass sub-cellular, cellular, and whole animal applications. Our innovations are being utilized around the world to enable new breakthroughs in research, health and medicine. Your local CRi agent or distributor is: Cambridge Research & Instrumentation, Inc. CRi 35-B Cabot Road Woburn, MA USA sales@cri-inc.com Website: Toll-Free (USA): Direct (Worldwide):
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