Differences in chromosome abnormalities in eggs and embryos between fertility centers Santi Munné
|
|
- Morgan McCarthy
- 6 years ago
- Views:
Transcription
1 Differences in chromosome abnormalities in eggs and embryos between fertility centers Santi Munné
2 disclosure None (lol) Except: - Chief Scientific Officer of CooperGenomics - Reprogenetics (PGS/PGD company), sold to Cooper - Recombine (Carrier Screening company), sold to Cooper - Phosphorus (genomics as a service) - MedAnswers (DTC advise on genetics and infertility) - Board advisors PreVivo (alternative to IVF)
3 Learning objectives 1) Not all PGS techniques are equal. Only hr-ngs can detect mosaics. 2) Aneuploidy increases with advancing maternal age but mosaicism does not 3) PGS v2 increases OPR: evidence from 5 CRTs 4) In addition of maternal age, IVF treatment can also induce chromosome abnormalities 5) PGS can be used as QC for optimizing ART procedures
4 Detection of Aneuploidy, segmentals and Mosaicism By hr-ngs
5 Comparison of current PGS platforms % embryos FISH qpcr acgh Embryo Vu SNP array hr-ngs Total Independent Data Signals* ,700 26,000 32, ,000 Resolution in Mb arm 20M 6M 20M 6M 3M Misdiagnosis aneuploides (a-f) 7% 1% 2% 3% d 2% 0% Unbalanced translocations (g) 2% custom no yes no yes yes Partial aneuploidies 5% no no yes some yes yes Polyploidy 2% yes no no no yes yes Mosaicism (h, i) 20% 20% no 4% no no 20% Miscarriage rate (j, k) 10-20% 20% 13% unk unk 11% a Gutierrez-Mateo et al (2011) Fertil Steril, b Scott et al. (2012), c Treff et al. (2012) Fertil Steril 97:819 24, d unpublished 7 misdiagnoses of 265 samples; e Kung et al. (2015) Reprod Biomed Online,, f Wells et al. (2014) J Med Genet, g Yeobah et al. (2015) ASRM, h Greco et al (2016) NEJM, i Tormasi et al (2015) PGDIS, ASRM. J Rodriguez-Purata et al. (2016) JARG; k Friedenthal et al. (2017) ESHRE * 24M reads per run, 24 samples per run, 30% reads lost = 700,000 reads per sample
6 Mosaicism: Common in day 3 embryos 30% of day-3 embryos were mosaic by FISH. The majority with all cells abnormal: 3[13]1[16]2[18]1[21]3[22] 1[13]1[16]1[18]1[21]1[22] <49% abnormal % abnormal % abnormal 528 1[13]1[16]2[18]2[21]1[22] 3[13]1[16]2[18]1[21]3[22] Munné S, Grifo J, Cohen J, Weier HUG Am J Hum Genet 1994; 55: Munné S, Weier HUG, Grifo J, Cohen J Biol. Reprod. 1994; 51: Colls et al. Fertil Steril 2007;88: [13]1[16]2[18]2[21]2[22] 1[13]1[16]2[18]2[21]1[22] 2[13]1[16]2[18]1[21]1[22] 1[16] 2[13]2[16]2[18]2[21]2[22] 2[13]3[18]1[21]1[22]
7 Higher dynamic range allows NGS to detect mosaics hr NGS Higher dynamic range acgh
8 High resolution NGS vs. acgh: Two center experiences acgh NGS p-value SET transfers Age NS IR (%) 63.9% 71.6% 0.01 OPR (%) 53.1% 61.9% Friedenthal, Maxwell, MD, Munné, Kramer, McCulloh, McCaffrey, Grifo (2017) ESHRE acgh NGS p-value transfers Age NS IR (%) 41.6% 57.8% <0.05 OPR (%) 44.0% 65.7% <0.02 Macer, Barritt, Surrey, Danzer, Ghadir, Wang, Pisarska (2017) PCRS
9 Base line chromosome abnormalities in blastocysts
10 Euploidy decreases with age but not with cohort size (hr-ngs) % euploid blastocysts * # of embryos produced < egg donors years years years years >42 years % 43% 38% 26% 17% 12% % 46% 38% 27% 17% 12% % 47% 39% 30% 16% 11% >10 60% 49% 42% 25% 19% 16% Total 59% 47% 42% 30% 19% 14% N = 42,217 embryos, and 7,425 cycles, Reprogenetics data to 9/2016, * excludes mosaics
11 Prognosis depending on age and ovarian response (hr-ngs) # of embryos produced egg donors % of patients with normal blastocysts <35 years years years years >42 years % 67% 57% 44% 29% 19% % 92% 87% 76% 56% 44% % 98% 96% 91% 73% 50% >10 100% 100% 99% 95% 89% 81% N = 42,217 embryos, and 7,425 cycles, Reprogenetics data to 9/2016, * excludes mosaics
12 N = 103,405 embryos. Reprogenetics and Genesis Genetics data to 1/2017 * Complex: >2 full abnormalities Abnormality rates by high resolution NGS Data from >100,000 embryos Egg donor < >42 Normal 59% 53% 44% 31% 19% 14% Mosaic 16% 18% 17% 13% 10% 8% Aneuploid (± mosaic) 18% 20% 28% 38% 41% 33% Complex (*) 7% 8% 10% 17% 28% 44% Polyploid 1% 1% 1% 1% 1% 1% Mosaics are MITOTIC and therefore do not increase with age Mosaics + Aneuploid and Mosaic show constant rates through age
13 Origin of Aneuploidies by karyomapping analysis Meiotic Errors 370 Maternal Origin Paternal Origin % 63 17% Gain Loss Gain Loss % % 61 Konstantinidis et al. (2016) ASRM 50.8% partial
14 maternal age and abnormalities detected by karyomapping analysis 100% 80% 60% 40% 20% 15% 36% 52% 24% 24% 26% Meiotic Errors Mitotic Errors 0% < > 40 Maternal Age Meiotic errors: Difference statistically significant at P<0.05 and P< Mitotic errors: No difference detected (P>0.05)
15 PGS v.2 Overall clinical results
16 1 st Randomized trial: acgh + fresh transfer, <35 years old Control PGS patients age <35 <35 replacement Day 6 Day 6 replaced 48 (1) 55 (1) Pregnancy rate 45.8% 70.9% P<0.05 Ongoing preg rate 41.7% 69.1% P<0.05 multiples 0 0 Yang et al. (2012) Molec Reprod
17 2 nd randomized trial: qpcr + fresh transfer Good prognosis patients (average 8 blastocysts) Control replaced on day 5, test biopsied on day 5 and replaced on day 6 PGD Control age N blastocysts Emb replaced implantation 79.8% 63.2% P=0.002 Sustained implant 66.4% 47.9% P=0.03 Delivery rate 84.7% 67.5% P=0.01 Scott et al., 2013 Fertil Steril.
18 3 rd randomized trial: 1 tested vs. 2 untested ongoing pregnancy rate 1 euploid 2 untested blastocyst blastocyst Fresh transfer 65% 70% NS Frozen transfer 55% 52% NS Forman et al. (2013) Fertil Steril Mean maternal age 35 (patients <43)
19 4th randomized trial: acgh but day 3 biopsy, patients y.o PGD-A Non PGD-A p-value No. of cycles performed Implantation rate (IR) 53% 28% < Clinical pregnancy rate/ transfer 54% 43% NS Clinical pregnancy rate/ patient 37% 39% NS Miscarriage rate (%) 3% 39% Ongoing IR 49% 15% < Delivery rate/transfer 53% 24% Delivery rate/patient 36% 22% Rubio et al. (2017) In Vitro Fertilization with Preimplantation Genetic Diagnosis for Aneuploidies (PGD-A) in Advanced Maternal Age: A Randomized Controlled Study. Fertil Steril, in press
20 Life birth rates / transfer SART 2014: Live Birth Rate per Transfer 60% 50% Not Tested PGD/PGS 40% 30% 20% 10% 0% < >42 Analyzed by David McCullough, PhD (NYU, NY)
21 Differences in euploidy rates between centers
22 Objective of the study Objective: To investigate if different fertility centers generate different aneuploidy rates. Problem: Aneuploidy rates could differ due to differences in maternal age and infertility indication. Solution: Differences in aneuploidy rates between Egg Donor cycles is probably ART treatment induced.
23 Aneuploidy rates per center in egg donors Confounders eliminated: - Egg donor age (excluded those >35 years old) - Infertility reason (all fertile) - Day of biopsy (day 5 and day 6 investigated) - Paternal age (using as proxy maternal age) - Other indications (male factor, sperm donation) - Cohort size (from <5 to >20 blastocysts) Confounders still present: - Center Munné et al. (2017) Human Reprod, in press
24 aneuploidy rates are significantly different between centers Cycles of PGS for egg donors: 1645 (13,595 blastocysts) Centers with >10 cycles: 42 Average egg donor (<35 yo): 25.5 Aneuploidy average: 31.0% Aneuploidy range: 18% to 60% p<0.001 Munné et al. (2017) Human Reprod, in press
25 aneuploidy rates are significantly different between centers 6 centers had below average rates and 3 above average rates Munné et al. (2017) Human Reprod, in press
26 Differences in other genetic abnormalities between centers
27 MOSAICISM: Significant difference between centers in egg donor embryos Center ID # N Euploid % Aneuploid % Mosaic % % 37% 16% % 26% 18% % 30% 24% % 25% 29% % 17% 31% % 17% 31% % 27% 33% % 19% 34% % 18% 44% 206 p= Sachdev et al. (2016) ASRM
28 PARTIAL ANEUPLOIDY: Significant Differences between centers Centers: 102 Partial aneuploidy: 0.5% (94/775) to 8% (9/116) (p<0.001) Full aneuploidy in donors: 18% to 43% (p<0.001)
29 MITOCHONDRIAL DNA: Significant difference between centers Embryos generated in 35 different IVF clinics in USA, and over 2000 euploid blastocysts Normal quantity Elevated quantity (low implantation)
30 What is causing these differences?
31 differences between doctors of the same center (study 1) 7 centers had several physicians with >6 egg donor cycles and PGS If doctors used same stimulation (5 centers): There were no differences in aneuploidy rates between physicians If doctors used different stimulation (2 centers): 1 centers with 4 physicians, 86 cycles, had 67% average euploidy rate - but one physician had 55% euploidy rate (p<0.05) 1 centers with 7 physicians, 406 cycles, had 68% average euploidy rate - but one physician had 74% euploidy rate (p<0.05) - another physician had 60% euploidy rate (p<0.05) - another physician had 57% euploidy rate (p<0.05) Munné et al. (2015) PGDIS
32 differences between doctors of the same center (study 2) Population: all patients (not only egg donors) years of age, year 2016, Test: PGS by hr-ngs Physician Total A B C D E Euploid (*) 40% 45% 38% 37% 42% 40% Simple Abnormal 29% 24% 30% 30% 25% 30% Complex Abnormal 11% 9% 13% 14% 9% 11% Mosaic (*) 21% 23% 19% 19% 24% 19% p<0.05 p<0.05 Sundheimer, Akopians, Al-Safi, Surrey, Danzer, Ghadir, Chang, Alexander, Barritt (submitted PCRS)
33 differences between doctors of the same center (study 3) Population: 6 doctors from same center, 423 PGS cycles of egg donation, PGS by acgh Comparison: correlation between euploidy rate per physician and controlled ovarian stimulation parameters such as: MII retrieved (MII), Estradiol on day of trigger (E2), gonadotrophin dose (dose/day), days of stimulation (days), donor age, blastocysts biopsiable (Bx), and number of euploid embryos (#Eup). Results: 1) Significant difference in euploidy rates between physicians (p<0.01) 2) Euploidy rates increased with more MIIs, fewer days, and lower dose/day: Log(#Eup) = x MII x days dose/day McCullogh, alikani, Munné (ASRM 2017) Differences between doctors regarding COH parameters were analyzed by ANOVA, and #Eup was compared to COH parameters using multiple regresion of COH parameters compared to log(#eup)
34 Culture media and chromosome abnormalities (4) KSOM + Sage + p complex protein HSA Fertilization 72% 70% NS #PN rate 5% 5% NS Blastulation 73% 76% NS Implantation,<38 y.o 38% 47% NS Biochem. Preg. loss 24% 7% p<0.025 % euploid embryos 16% 29% p<0.025 ph Values ??? C. Hickman, D. Wells, D. Gwinnett, T. Wilkinson, S. Christiansen, O. Oliana, B. Abramov, A. Carby, S. Lavery (2016) Euploid rate sensitivity to laboratory culture environment: a blind, prospective, randomised, sibling study. Human Reproduction 31; Supp 1: i ; P-203
35 Culture media and mosaicism (5) 27 fertility centers answered a questionaire about culture conditions: Media culture, oxygen in incubators, type of incubator, etc. Mosaicism rates ranged from 6% to 31% Mosaicism rates were correlated with type of media culture: Mosaicism Continuous media: 20% Sequential media: 10% (p<0.001) E. Fragouli, S. Alfarawati, K. M. Simpkins, G. Cutts, K Spath, D. Babariya, N. Kubikova L. Rubistello, S. Munné, D. Wells (2017) ESHRE and unpublished data
36 Summary of potential causes Controlled Ovarian Stimulation (COH): - Live birth rate decreases with increasing Gonadotropin dose (1) - Differences between doctors linked to dosage and duration of COH (2) Size of follicles chosen: - Smaller follicles produce more multinucleated embryos (3) Laboratory conditions: - Culture Media (4,5) - Ph, Temperature (6) (1) Baker et al. (2016) Fertil Steril 104: , (2) McCullogh, Alikani, Munné (2017) submitted ASRM, (3) De Vincentiis (2013) Fertil Steril 99:414 21, (4) Hickmanet al.(2016) Human Reproduction 31; Supp 1: i , (5) Fragouli, Alfarawati, Simpkins, Cutts, Spath, Babariya, Kubikova, Rubistello, Munné, Wells (2017) ESHRE
37 Scientists Santi Munné, PhD, CSO (US) Mark Hughes, MD, PhD (US) Jacques Cohen, PhD (US) Dagan Wells, PhD (UK) Elpida Fragouli, PhD (UK) Joson Horcajadas, PhD (LATAM) M. Konstantinidis, PhD (US) Samer Alfarawati, PhD (UK) Tomas Escudero, MSc (US) Josh Blazek, PhD (US) Mike Large, PhD (US) Katharina Spath, PhD (UK) Ryan Subaran, PhD (US) Sarthak Sawarkar, MSc (US) Dhruti Babariya, PhD (UK) Pere Colls, PhD (US) Tony Gordon, PhD (UK) John Kitchen, PhD (US) Carles Gimenez, PhD (Spain) Mireia Sandalinas, PhD (Spain) Sophia Tormasi, MSc (US) Lia Ribustello, MSc (US) Katie Bauckman, MSc (US) Renata Prates, MSc (US) Luis Guzman, PhD (Peru) Muriel Roche, PhD (Japan) Dr. Araki, PhD (Japan) Bioinformatics, VC scientists Arun Manoharan, PhD (US) Avinash Shanmugan, PhD (US) Ursula Schick, PhD (US) Lauren Hurd, PhD (US) Jim Hayes, PhD (US) Eric Proffitt, PhD (US) Genetic Councilors (R&D) Amy Jordan Erin Mills Rachael Cabey Dina Goldberg Haley Nisson
Mosaicism in human embryos: Etiology and pregnancy outcome Santi Munné
Mosaicism in human embryos: Etiology and pregnancy outcome Santi Munné disclosure - Chief Scientific Officer of CooperGenomics - Founder @ Reprogenetics (PGS/PGD company), sold to Cooper - Founder @ Recombine
More informationNew methods for embryo selection: NGS and MitoGrade
New methods for embryo selection: NGS and MitoGrade Santiago Munné, PhD US: Livingston, Los Angeles, Chicago, Portland, Miami / Europe: Barcelona (Spain), Oxford (UK), Hamburg (Germany) / Asia: Kobe (Japan),
More informationUSA: Livingston, NJ. PGD for infertility. Europe: Barcelona, Spain Oxford, UK Hamburg, Germany. Asia: Kobe, Japan. South America: Lima, Peru
PGD for infertility Santiago Munné USA: Livingston, NJ Europe: Barcelona, Spain Oxford, UK Hamburg, Germany Asia: Kobe, Japan South America: Lima, Peru The majority of embryos with good morphology are
More informationIndications for chromosome screening Dagan Wells, PhD, FRCPath dagan.wells@obs-gyn.ox.ac.ukgyn.ox.ac.uk Chromosome imbalance (aneuploidy) Uncontroversial data The incidence of aneuploidy Aneuploidy is
More informationComprehensive Chromosome Screening Is NextGen Likely to be the Final Best Platform and What are its Advantages and Quirks?
Comprehensive Chromosome Screening Is NextGen Likely to be the Final Best Platform and What are its Advantages and Quirks? Embryo 1 Embryo 2 combine samples for a single sequencing chip Barcode 1 CTAAGGTAAC
More informationChromosomal Aneuploidy
The Many Advantages of Trophectoderm Biopsy Compared to Day 3 Biopsy for Pre- Implantation Genetic Screening (PGS) Mandy Katz-Jaffe, PhD Chromosomal Aneuploidy Trisomy 21 Fetus Aneuploidy is the most common
More informationIVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW
IVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW Jon Havelock, MD, FRCSC, FACOG Co-Director - PCRM Disclosure No conflict of interest in relation
More informationPGS & PGD. Preimplantation Genetic Screening Preimplantation Genetic Diagnosis
1 PGS & PGD Preimplantation Genetic Screening Preimplantation Genetic Diagnosis OUR MISSION OUR MISSION CooperGenomics unites pioneering leaders in reproductive genetics, Reprogenetics, Recombine, and
More informationSNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation carrier and normal blastocysts
J Assist Reprod Genet (2016) 33:1115 1119 DOI 10.1007/s10815-016-0734-0 TECHNOLOGICAL INNOVATIONS SNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation
More informationIncrease your chance of IVF Success. PGT-A Preimplantation Genetic Testing for Aneuploidy (PGS 2.0)
Increase your chance of IVF Success PGT-A Preimplantation Genetic Testing for Aneuploidy (PGS 2.0) What is PGT-A? PGT-A, or Preimplantation Genetic Testing for Aneuploidy (PGS 2.0), is a type of genomic
More informationNew perspectives on embryo biopsy, not how, but when and why PGS
New perspectives on embryo biopsy, not how, but when and why PGS Kangpu Xu, PhD Director, Laboratory of Preimplantation Genetics Center for Reproductive Medicine Weill Cornell Medical College of Cornell
More informationTargeted qpcr. Debate on PGS Technology: Targeted vs. Whole genome approach. Discolsure Stake shareholder of GENETYX S.R.L
Antonio Capalbo, PhD Laboratory Director GENETYX, reproductive genetics laboratory, Italy PGT responsible GENERA centers for reproductive medicine, Italy Debate on PGS Technology: Targeted vs. Whole genome
More informationThe Impact of ESHRE 2017 on Japanese Fertility Practice
The Impact of ESHRE 2017 on Japanese Fertility Practice This resource is supported by an educational grant from Merck KGaA, Darmstadt, Germany. The GWHA was interested in the opinions of practicing clinicians
More informationFuture of Genetic testing in fertility
Future of Genetic testing in fertility Sarthak S Sawarkar CooperGenomics University of Kent 6/8/2018 1 CONTENTS 1. Introduction to genetic testing 2. PGD for gene defects 3. PGS for aneuploidy 4. PGS version
More informationDiagnosis of parental balanced reciprocal translocations by trophectoderm biopsy and comprehensive chromosomal screening
Diagnosis of parental balanced reciprocal translocations by trophectoderm biopsy and comprehensive chromosomal screening Lian Liu, MD Co-Authors: L. W. Sundheimer1, L. Liu2, R. P. Buyalos1,3, G. Hubert1,3,
More informationCongreso Nacional del Laboratorio Clínico 2016
Actualización en Screening Genético Preimplantacional Maria Giulia Minasi Center for Reproductive Medicine European Hospital Rome, Italy Aneuploidy rate can reach 60% in human embryos Aneuploidy increases
More informationFertility 101. About SCRC. A Primary Care Approach to Diagnosing and Treating Infertility. Definition of Infertility. Dr.
Dr. Shahin Ghadir A Primary Care Approach to Diagnosing and Treating Infertility St. Charles Bend Grand Rounds November 30, 2018 I have no conflicts of interest to disclose. + About SCRC State-of-the-art
More informationRejuvenation of Gamete Cells; Past, Present and Future
Rejuvenation of Gamete Cells; Past, Present and Future Denny Sakkas PhD Scientific Director, Boston IVF Waltham, MA, USA Conflict of Interest I have no conflict of interest related to this presentation.
More informationProblem Challenge Need. Solution Innovation Invention
Problem Challenge Need Solution Innovation Invention Tubal Infertility In-vitro Fertilisation Steptoe and Edwards Birth after the reimplantation of a human embryo. Lancet 1978 Louise Brown, 25. Juli 1978
More informationMyths About Success Rates Do they truly reflect quality of care
Myths About Success Rates Do they truly reflect quality of care Barry Behr PhD., HCLD Professor and IVF Laboratory Director Division of Reproductive Endocrinology and Infertility Dept OB/GYN Stanford University
More informationComprehensive chromosome screening and embryo biopsy: advantages and difficulties. Antonio Capalbo, PhD Italy
Comprehensive chromosome screening and embryo biopsy: advantages and difficulties Antonio Capalbo, PhD Italy Disclosure Antonio Capalbo, PhD GEERA, Reproductive medicine centers GEETYX, molecular genetics
More informationNothing more controversial than PGS
Nothing more controversial than PGS Norbert Gleicher, MD M e d i c a l D i r e c t o r a n d C h i e f S c i e n t i s t, C e n t e r F o r H u m a n R e p ro d u c t i o n, N e w Yo r k, N Y P r e s i
More informationScientific and Clinical Advances Advisory Committee Paper
Scientific and Clinical Advances Advisory Committee Paper Paper title Paper number SCAAC(06/15)07 Meeting date 10 June 2015 Agenda item 7 Author Information/decision Resource implications Implementation
More informationThe effects of PGS/PGT-A on IVF outcomes
The effects of PGS/PGT-A on IVF outcomes Raoul Orvieto M.D. - Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Ramat Gan, Israel - The Tarnesby-Tarnowski Chair for Family Planning and
More informationNEXCCS. Your guide to aneuploidy screening
NEXCCS Your guide to aneuploidy screening GROWING FAMILIES What is comprehensive chromosome screening? Comprehensive chromosome screening (CCS), also known as preimplantation genetic screening (PGS) or
More informationThe ASRM Committee Opinion on PGT for Aneuploidy Our Conclusions and How We Drew Them. Alan Penzias, MD
The ASRM Committee Opinion on PGT for Aneuploidy Our Conclusions and How We Drew Them Alan Penzias, MD Chair, Practice Committee American Society for Reproductive Medicine Development of US National Guidelines:
More information% Oocyte Donation Pregnancyes (days 3)
Ovulation induction in oocyte donors Roma- September 2007 Dr. José Remohí Dr. Carmen Rubio Dr. Amparo Mercader Dr. Pilar Alama Dr. Marco Melo Evolution of oocyte donation cycles 1500 1500 1000 58% 661
More informationHold On To Your Dreams
Hold On To Your Dreams Dr. Michael Kettel Dr. Sandy Chuan 1. THE BASICS OF IVF & EMBRYO DEVELOPMENT 2. IVF ADD-ONS - MYTH VS. SCIENCE IN VITRO FERTILIZATION 1. Ovarian Stimulation 2. Egg Retrieval 3. Create
More informationL6: DuoStim: the alternative of oocytes/embryos accumulation programs Carlo Alviggi
L6: DuoStim: the alternative of oocytes/embryos accumulation programs Carlo Alviggi Italy What is the aim of IVF? What is the measure of success in IVF? Cumulative live birth rate per started cycle Live
More informationValidation of Next-Generation Sequencer for 24-Chromosome Aneuploidy Screening in Human Embryos
GENETIC TESTING AND MOLECULAR BIOMARKERS Volume 21, Number 11, 2017 ª Mary Ann Liebert, Inc. Pp. 1 7 DOI: 10.1089/gtmb.2017.0108 ORIGINAL ARTICLE Validation of Next-Generation Sequencer for 24-Chromosome
More informationDiagnostic Techniques to Improve the Assessment of Human IVF Embryos: Genomics and Proteomics
Diagnostic Techniques to Improve the Assessment of Human IVF Embryos: Genomics and Proteomics Mandy G Katz-Jaffe Introduction A fundamental component of assisted reproductive technologies (ART) is the
More informationComprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study
ORIGINAL ARTICLES: ASSISTED REPRODUCTION Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study Richard T. Scott
More informationExplaining the Purpose of PGT-A. Nathan R. Treff PhD, HCLD(ABB) Chief Science Officer Clinical Laboratory Director
Explaining the Purpose of PGT-A Nathan R. Treff PhD, HCLD(ABB) Chief Science Officer Clinical Laboratory Director Disclosures Cofounder, Shareholder and CSO, Genomic Prediction Inc Director, Genomic Prediction
More informationUNDERSTANDING THE GENETIC HEALTH OF EMBRYOS
UNDERSTANDING THE GENETIC HEALTH OF EMBRYOS What is preimplantation genetic testing for aneuploidy? (an abnormal number of chromosomes; PGT-A) is a testing technique that can help choose embryos that appear
More informationIncidence of Chromosomal Abnormalities from a Morphologically Normal Cohort of Embryos in Poor- Prognosis Patients
Incidence of Chromosomal Abnormalities from a Morphologically Normal Cohort of Embryos in Poor- Prognosis Patients M. C. MAGLI,1 L. GIANAROLI,1,3 S. MUNNE,2 and A. P. FERRARETTI1 Submitted: December 29,
More informationPreimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC
Preimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC Overview Discuss what PGD and PGS are Pt examples What we have learned Where
More informationPre-implantation genetic diagnosis and pre-implantation genetic screening: two years experience at a single center
Original Article Obstet Gynecol Sci 2018;61(1):95-101 https://doi.org/10.5468/ogs.2018.61.1.95 pissn 2287-8572 eissn 2287-8580 Pre-implantation genetic diagnosis and pre-implantation genetic screening:
More informationDo it Once, Do it Right
Do it Once, Do it Right Craig Reisser Andrea Speck-Zulak Families Through Surrogacy 2016 Founded in 1989 - more than 25 years building families patients from 40 countries One of the largest IVF clinics
More information@ CIC Edizioni Internazionali. Origin and mechanisms of aneuploidies in preimplantation embryos
Review article Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives Antonio Capalbo 1 Cristina Poggiana 1 Cristina Patassini 1 Anna Cecchele 1 Emiliano Scepi 1
More informationCIC Edizioni Internazionali. Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives. Summary.
Mini-review Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives Antonio Capalbo 1 Cristina Poggiana 2 Cristina Patassini 2 Anna Checchele 2 Emiliano Scepi 2 Danilo
More informationDisclosure. Dagan Wells University of Oxford Oxford, United Kingdom
Disclosure Dagan Wells University of Oxford Oxford, United Kingdom Disclosure Declared to be member of the advisory board, board of directors or other similar groups of Illumina Objectives Consider Aneuploidy
More informationEffect of ovarian stimulation on oocyte quality and embryonic aneuploidy: a prospective, randomised controlled trial
FULL PROJECT TITLE: Effect of ovarian stimulation on oocyte quality and embryonic aneuploidy: a prospective, randomised controlled trial (STimulation Resulting in Embryonic Aneuploidy using Menopur (STREAM)
More informationPregnancy outcomes following 24-chromosome preimplantation genetic diagnosis in couples with balanced reciprocal or Robertsonian translocations
Pregnancy outcomes following 24-chromosome preimplantation genetic diagnosis in couples with balanced reciprocal or Robertsonian translocations Dennis Idowu, M.D., a Katrina Merrion, M.S., b Nina Wemmer,
More informationValidation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos
Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos Cristina Gutierrez-Mateo, Ph.D., a Pere Colls, Ph.D., a Jorge Sanchez-Garcıa, Ph.D., a Tomas
More informationUnderstanding IVF Processes in Surrogacy
Melvin H. Thornton II MD Medical Director CT Fertility Understanding IVF Processes in Surrogacy The Basics Surrogacy involves multiple parties IVF CLINIC Egg donors screening and matching* Medical process
More informationA Stepwise Approach to Embryo Selection and Implantation Success
Precise Genetic Carrier Screening An Overview A Stepwise Approach to Embryo Selection and Implantation Success Put today s most advanced genetic screening technology to work for you and your family s future.
More informationINSIDE IVF: HOW SCIENCE CARES FOR PATIENTS DR DEIRDRE ZANDER-FOX MONASH IVF GROUP HDA GRAND ROUND OCTOBER 31 ST 2018
INSIDE IVF: HOW SCIENCE CARES FOR PATIENTS DR DEIRDRE ZANDER-FOX MONASH IVF GROUP HDA GRAND ROUND OCTOBER 31 ST 2018 IVF-THE ULTIMATE GOAL FERTILISATION EMBRYO CLEAVAGE AND DEVELOPMENT POSITIVE HCG POSITIVE
More informationWhat is the POSEIDON concept?
Improving Success in ART: how to define it and key strategies to get the best outcomes, Kiev, 21-09-2018 What is the POSEIDON concept? Alessandro Conforti Italy Objectives Unravel the definition of POR
More informationApplication of OMICS technologies on Gamete and Embryo Selection
Application of OMICS technologies on Gamete and Embryo Selection Denny Sakkas, Ph.D. Scientific Director, Boston IVF Waltham, MA, USA THE FUTURE ROLE OF THE EMBRYOLOGIST WILL FOCUS ON PROVIDING OUR PATIENTS
More information1 PGS (PGS) IVF PGS, PGS,, PGS 2, PGS : (PGS); PGS; ; : R321.1 : A : X(2015) PGS 1995, 1 , (ART)
35 2 Vol.35 No.2 2015 2 Feb. 2015 Reproduction & Contraception doi: 10.7669/j.issn.0253-357X.2015.02.0114 E-mail: randc_journal@163.com 1 123 (1. 200011) (2. 200011) (3. 200011) () IVF 2 : () : R321.1
More informationEmbryoCellect TM. Pre-implantation Genetic Screening Kit TECHNICAL INFORMATION
EmbryoCellect TM Pre-implantation Genetic Screening Kit TECHNICAL INFORMATION Aneuploidy Whole chromosome aneuploidy has been shown to affect all chromosomes in IVF embryos. Aneuploidy is a significant
More informationAn Update on PGD: Where we are today
An Update on PGD: Where we are today Joyce Harper UCL Centre for PG&D and CRGH Institute for Womens Health University College London Overview What is PGD/PGS How we do it Disadvantages and advantages Future
More informationbut it still needs a bit of work
but it still needs a bit of work jc@embryos.net Reprogenetics ART Institute of Washington Life Global Principle investigator of cytoplasmic transfer series (1996-2001) Is there an alternative to MRT? Lessons
More informationPreimplantation Genetic Testing
Protocol Preimplantation Genetic Testing (40205) Medical Benefit Effective Date: 01/01/14 Next Review Date: 09/14 Preauthorization No Review Dates: 09/11, 09/12, 09/13 The following Protocol contains medical
More informationIntroduction ASSISTED REPRODUCTION TECHNOLOGIES. Alison Coates 1,2 & Brandon J. Bankowski 1 & Allen Kung 2,3 & Darren K. Griffin 2 & Santiago Munne 3
J Assist Reprod Genet (2017) 34:71 78 DOI 10.1007/s10815-016-0832-z ASSISTED REPRODUCTION TECHNOLOGIES Differences in pregnancy outcomes in donor egg frozen embryo transfer (FET) cycles following preimplantation
More informationUCLA UCLA Previously Published Works
UCLA UCLA Previously Published Works Title Recent advances in preimplantation genetic diagnosis and screening. Permalink https://escholarship.org/uc/item/6gc712qc Journal Journal of assisted reproduction
More informationEuploidy rates in donor egg cycles significantly differ between fertility centers
Human Reproduction, Vol.32, No.4 pp. 743 749, 2017 Advanced Access publication on March 2, 2017 doi:10.1093/humrep/dex031 ORIGINAL ARTICLE Embryology Euploidy rates in donor egg cycles significantly differ
More informationCase Report What Drives Embryo Development? Chromosomal Normality or Mitochondria?
Hindawi Case Reports in Genetics Volume 2017, Article ID 4397434, 4 pages https://doi.org/10.1155/2017/4397434 Case Report What Drives Embryo Development? Chromosomal Normality or Mitochondria? A. Bayram,
More informationUniversal Embryo Cryopreservation: Frozen versus Fresh Transfer. Zaher Merhi, M.D.
Universal Embryo Cryopreservation: Frozen versus Fresh Transfer Zaher Merhi, M.D. Disclosure: None Fewer complications with IVF 1.5% children in US are born through ART 1.1 million children since 2006
More information24sure TM Setting new standards in IVF
24sure TM Setting new standards in IVF 24sure TM The clinical challenge While in vitro fertilization (IVF) is a highly successful medical intervention that has revolutionized the treatment of infertility,
More informationNext generation technologies for PGD and 24- chromosome aneuploidy testing. PGD s 25 Year Journey: What is next?
Scientific Program 10 May 2015- Sunday 08:30 Pre-Congress Course Next generation technologies for PGD and 24- chromosome aneuploidy testing Sponsored by Illumina (invite only) 14:00 Illumina Focus Group
More informationResults of the Virtual Academy of Genetics (VAoGEN) questionnaire on Mosaicism in PGS
Results of the Virtual Academy of Genetics (VAoGEN) questionnaire on Mosaicism in PGS Ariel Weissman, MD IVF Unit, Dep. Ob/Gyn Wolfson Medical Center, Holon Sackler Faculty of Medicine, Tel Aviv University
More informationScientifically advanced. Personally accessible.
Scientifically advanced. Personally accessible. EmbryVu. Advanced preimplantation genetic screening that can help you find the path to pregnancy. The power to decide When you are going through treatment
More informationBlastocentesis: innovation in embryo biopsy
Blastocentesis: innovation in embryo biopsy L. Gianaroli, MC Magli, A. Pomante, AP Ferraretti S.I.S.Me.R. Reproductive Medicine Unit, Bologna, Italy Bologna, 8-11 May 2016 www.iiarg.com www.sismer.it 2013
More informationAbstract. Introduction
RBMOnline - Vol 13 No 6. 2006 869-874 Reproductive BioMedicine Online; www.rbmonline.com/article/2507 on web 18 October 2006 Article Preimplantation genetic diagnosis significantly improves the pregnancy
More informationINDICATIONS OF IVF/ICSI
PROCESS OF IVF/ICSI INDICATIONS OF IVF/ICSI IVF is most clearly indicated when infertility results from one or more causes having no other effective treatment; Tubal disease. In women with blocked fallopian
More informationPOSEIDON s stratification of Low prognosis patients in ART and its new proposed marker of successful outcome: The WHY, the WHAT, and the HOW
POSEIDON s stratification of Low prognosis patients in ART and its new proposed marker of successful outcome: The WHY, the WHAT, and the HOW Sandro C. Esteves, MD., PhD. I. The WHY The management of patients
More informationHsing-Hua Lai 1, Tzu-Hsuan Chuang 1, Lin-Kin Wong 1, Meng-Ju Lee 1, Chia-Lin Hsieh 1, Huai-Lin Wang 1 and Shee-Uan Chen 2*
Lai et al. Molecular Cytogenetics (2017) 10:14 DOI 10.1186/s13039-017-0315-7 RESEARCH Open Access Identification of mosaic and segmental aneuploidies by next-generation sequencing in preimplantation genetic
More informationPreimplantation genetic diagnosis
Preimplantation genetic diagnosis Borut Peterlin Clinical institute of medical genetics, University Medical Centre Ljubljana Outline of the presentation Primary prevention of genetic diseases Motivation
More informationMALBAC Technology and Its Application in Non-invasive Chromosome Screening (NICS)
MALBAC Technology and Its Application in Non-invasive Chromosome Screening (NICS) The Power of One Adapted from Internet Single Cell Genomic Studies Ultra Low Sample Input Advances and applications of
More informationTrends in Egg Donation. Vitaly A. Kushnir MD Center for Human Reproduction
Trends in Egg Donation Vitaly A. Kushnir MD Center for Human Reproduction Disclosures No relevant financial relationships to disclose CHR views the commercial trade in human oocytes with considerable ethical
More informationProgesterone and clinical outcomes
Synchronization of Slowly Developing Embryos Restores Implantation Success Richard T. Scott, Jr, MD, HCLD Clinical and Scientific Director, Reproductive Medicine Associates of New Jersey Professor and
More informationArticles Impact of parental gonosomal mosaicism detected in peripheral blood on preimplantation embryos
RBMOnline - Vol 5. No 3. 306 312 Reproductive BioMedicine Online; www.rbmonline.com/article/699 on web 12 September Articles Impact of parental gonosomal mosaicism detected in peripheral blood on preimplantation
More informationTelomeres and Genomic Instability In Preimplanation Embryos. David L. Keefe, M.D.
Telomeres and Genomic Instability In Preimplanation Embryos David L. Keefe, M.D. I. Global Genomic Instability, Including Aneuploidy, Mosaicism & Copy Number Variants, is Common In Preimplantation Embryos
More informationZygotes showing a single pronucleus
In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo, M.Sc., a Monica Parriego, M.Sc., a Montserrat Boada,
More informationERA. Endometrial Receptivity Analysis. Patented since
Endometrial Receptivity Analysis Patented since 2009 www.igenomix.com ERA ERA analyzes by NGS the expression of 236 genes in order to determine the personalized window of implantation for each patient.
More informationOriginal Policy Date
MP 2.04.77 Preimplantation Genetic Testing Medical Policy Section OB/Gyn/Reproduction Issue 12:2013 Original Policy Date 12:2013 Last Review Status/Date Reviewed with literature search/12:2013 Return to
More informationPGS Embryo Screening
PGS Embryo Screening Contents What are chromosomes? 3 Why should I consider chromosome testing of my embryos? 3 Embryo testing using preimplantation genetic screening (PGS) 4 How does PGS and the chromosome
More informationSame Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method
Same Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method Presenter: Dr. Ali Hellani, Founder, Viafet Genomic Center, Dubai Wednesday,
More informationTechnical Update: Preimplantation Genetic Diagnosis and Screening
No. 323, May 2015 (Replaces No. 232, August 2009) Technical Update: Preimplantation Genetic Diagnosis and Screening This technical update has been prepared by the Genetics Committee and approved by the
More informationNumber of oocytes and live births in IVF
Number of oocytes and live births in IVF Dr Sesh K Sunkara MD, MRCOG Royal Marsden Hospital, London Kings Healthcare Partners (Guy s & St Thomas NHS Foundation Trust), London, UK Background IVF results
More informationPatient Price List. t: e: w:
Patient Price List t: 0333 015 9774 e: enquires@ivi.uk w: www.ivi.uk fertility treatments Pre Treatment Medical Consultation 250 Nurse Planning 200 Baseline ultrasound scan of uterus and ovaries included
More informationNew Innovations and Technologies:
New Innovations and Technologies: How and When in the Fertility Clinic? Prof Darren K Griffin (Biosciences); Prof Sally Sheldon (Law) Centre for Interdisciplinary Studies of Reproduction (CISoR) University
More informationS.Kahraman 1,4, M.Bahçe 2,H.Şamlı 3, N.İmirzalıoğlu 2, K.Yakısn 1, G.Cengiz 1 and E.Dönmez 1
Human Reproduction vol.15 no.9 pp.2003 2007, 2000 Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation
More informationA Tale of Three Hormones: hcg, Progesterone and AMH
A Tale of Three Hormones: hcg, Progesterone and AMH Download the Ferring AR ipad/iphone app from the Apple Store: http://bit.ly/1okk74m Interpreting Follicular Phase Progesterone Ernesto Bosch IVI Valencia,
More informationPreimplantation genetic diagnosis: polar body and embryo biopsy
Human Reproduction, Vol. 15, (Suppl. 4), pp. 69-75, 2000 Preimplantation genetic diagnosis: polar body and embryo biopsy Luca Gianaroli SISMER, Via Mazzini 12, 40138 Bologna, Italy Scientific Director
More informationKatz MG, Trounson AO and Cram DS. (2002) DNA fingerprinting of sister blastomeres from human IVF embryos. Human Reproduction 17:
Peer Reviewed International Publications Katz MG & Vollenhoven B. (2000) The endocrine reproductive consequences of anorexia nervosa. British Journal of Obstetrics and Gynaecology 107: 707-713. Katz MG,
More informationArticle Preimplantation genetic diagnosis of numerical abnormalities for 13 chromosomes
RBMOnline - Vol 6. No 2. 226 231 Reproductive BioMedicine Online; www.rbmonline.com/article/794 on web 28 January 2003 Article Preimplantation genetic diagnosis of numerical abnormalities for 13 chromosomes
More informationThe relationship between blastocyst morphology, chromosomal abnormality, and embryo gender
IN VITRO FERTILIZATION The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender Samer Alfarawati, M.S. a,b Elpida Fragouli, Ph.D., a,b Pere Colls, Ph.D., c John Stevens,
More informationHow to make the best use of the natural cycle for frozen-thawed embryo transfer?
How to make the best use of the natural cycle for frozen-thawed embryo transfer? Ariel Weissman, MD IVF Unit, Dep. Ob/Gyn Wolfson Medical Center, Holon Sackler Faculty of Medicine, Tel Aviv University
More informationAlexia Chatziparasidou, Martine Nijs, Martha Moisidou, Oraiopoulou Chara, Christina Ioakeimidou, Christos Pappas, Nicos Christoforidis
SHORT RESEARCH ARTICLE Accumulation of oocytes and/or embryos by vitrification: a new strategy for managing poor responder patients undergoing pre implantation diagnosis [version 2; referees: 2 approved,
More informationValidation of a next-generation sequencing based protocol for 24-chromosome aneuploidy screening of blastocysts
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 Q3 Q1 Q2 ORIGINAL ARTICLE: GENETICS
More informationFreeze-All Policy: Is It Right for Everyone?
Transcript Details This is a transcript of an educational program accessible on the ReachMD network. Details about the program and additional media formats for the program are accessible by visiting: https://reachmd.com/online-education/non-certified-non-accredited/freeze-all-policy-it-righteveryone/9879/
More informationHonorary Fellow of the Royal College of Obs. & Gyn. First Indian to receive FIGO s Distinguished Merit Award for Services towards women s health.
Prof.Duru Shah Founder President The PCOS Society (India) President Elect of the Indian Society for Assisted Reproduction (ISAR) Honorary Fellow of the Royal College of Obs. & Gyn. First Indian to receive
More informationThe work of a fertility specialist Steven Fleming PhD Honorary Associate, University of Sydney Director of Embryology, ORIGIO a/s
The work of a fertility specialist Steven Fleming PhD Honorary Associate, University of Sydney Director of Embryology, ORIGIO a/s sfleming@origio.com Scope of work Evaluation and diagnosis of the infertile
More informationRandomized comparison of next-generation sequencing and array comparative genomic hybridization for preimplantation genetic screening: a pilot study
Yang et al. BMC Medical Genomics (2015) 8:30 DOI 10.1186/s12920-015-0110-4 RESEARCH ARTICLE Open Access Randomized comparison of next-generation sequencing and array comparative genomic hybridization for
More informationSNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH
Molecular Human Reproduction, Vol.16, No.8 pp. 583 589, 2010 Advanced Access publication on May 19, 2010 doi:10.1093/molehr/gaq039 NEW RESEARCH HORIZON Review SNP microarray-based 24 chromosome aneuploidy
More informationPATIENT CONSENT FORM Preimplantation Genetic Screening (PGS) 24 Chromosome Aneuploidy and Translocation Screening with acgh
PREIMPLANTATION GENETIC SCREENING FOR ANEUPLOIDY SCREENING INTRODUCTION Preimplantation genetic screening (PGS) is used in conjunction with in-vitro fertilization (IVF) to screen embryos for numerical
More informationPreimplantation Genetic Diagnosis (PGD) in Western Australia
Preimplantation Genetic Diagnosis (PGD) in Western Australia Human somatic cells have 46 chromosomes each, made up of the 23 chromosomes provided by the egg and the sperm cell from each parent. Each chromosome
More informationAccuracy of FISH analysis in predicting chromosomal status in patients undergoing preimplantation genetic diagnosis
Accuracy of FISH analysis in predicting chromosomal status in patients undergoing preimplantation genetic diagnosis Catherine M. DeUgarte, M.D., a Man Li, M.D., Ph.D., b Mark Surrey, M.D., c Hal Danzer,
More information