REPRODUCTIVE BIOTECHNOLOGY IN SWINE

Size: px
Start display at page:

Download "REPRODUCTIVE BIOTECHNOLOGY IN SWINE"

Transcription

1 REPRODUCTIVE BIOTECHNOLOGY IN SWINE

2 References * Animal breeding and infertility by M. J. Meredith * Controlled reproduction in pigs by I. Gordon * Reproduction in farm animals by E.S.E. Hafez * Progress in reproductive biotechnology in swine Theriogenology 56: * Transgenic technology and application in swine Theriogenology 56:

3 Factors affecting reproductive efficiency * Early development of the pig conceptus * Genetic and environmental factors * Nutrition and sow reproduction * Breeds * Fertility and temperature * Sow culling strategies * Stress, records and reproduction * Effect of antibiotics and hormones

4 Control of estrus Breeding pigs at younger ages Fixed-time artificial insemination Embryo transfer Controlled reproduction in pigs Pregnancy testing Increasing litter size Control of farrowing More frequent farrowings

5 Biotechnology in livestock * Reproductive biotechnological procedures * Molecular genetics

6 Reproductive biotechnological procedures Artificial insemination (AI) Estrus synchronization Induction of parturition Embryo transfer (ET) Cryopreservation of oocytes and embryos Sperm sexing In vitro production of embryos (IVMFC) Embryo bisection Nuclear transfer Microinjection of DNA constructs

7 Molecular genetics Genome analysis e.g. sequencing, mapping and determination of polymorphisms of porcine genes Molecular diagnostics e.g. identify genetic disorders, identity and/or diversity Functional genomics e.g. expression patterns, interaction of genes Transgenic modification e.g. gain or loss of function

8 Animal biotechnology the first live born offspring in swine Technology AI (fresh) AI (frozen/thawed) ET Embryo freeze Sexing IVF Embryo bisection Blastomere proliferation Nuclear transfer Transgenic Year of publication (contr. fr., hatch. bl.); 1995 (z.p.i.) 1991 (SI); 1997 (IVF) 1985; 1989 (IVM) 1985 (4-cell); 1988 (mo./bl.) (blastomere); 2000 (somatic cells) 1985 (microinjection) Authors Rodin and Lipatow Polge et al. Kvasnickii Hayashi et al. Nagashima et al. Johnson; Rath et al. Cheng; Mattioli et al. Polge; Nagashima et al. Saito and Niemann Prather et al. Polejaeva et al. Hammer et al.

9 Factors affecting semen quality and sex driven in boars Effects of nutritional and environmental factors Space requirements Light programes and boar reproduction Fertility problems in the boar Boar culling rate Efficiency of the mating process Overuse and underuse of the boar Boar stimuli at mating time Endocrine factors in boar sperm production

10 Reproductive biotechnological procedures Artificial insemination (AI) Estrus synchronization Induction of parturition Embryo transfer (ET) Cryopreservation of oocytes and embryos Sperm sexing In vitro production of embryos (IVM/F/C) Embryo bisection Nuclear transfer Microinjection of DNA constructs

11 Advantages of AI Genetic improvement e.g., widespread use of outstanding sires; improving accuracy of selection through progeny test; permitting crossbreeding; introduction of new genetics Control of venereal diseases Availability of accurate breeding records Economic service Safety through elimination of dangerous male Use of deep-frozen semen after a donor is dead Gender control

12 Sex of offspring Genetic sex Nuclear transfer Parthenogenetic activation of ova Fusion of two oocytes

13 Differences between X and Y sperm * DNA size * Shape of sperm * Weight * Density * Identity motility * Surface charge/biochemistry * Internal biochemistry

14 The degree of differences may be affected by age of semen repeat breeding co-twin with heifers

15 Valid laboratory methods to separate X and Y sperm * Albumin separation yielding 75-80% Y sperm * Sephadex filtration yielding 70-75% Y sperm

16 Methods used : transcervical insemination surgical insemination AI is facilitated with estrus synchronization programs * Extending the luteal phase * Shortening the luteal phase

17 The basic requirements for embryo transfer A source of embryos A reliable method of transferring the embryos Suitably synchronized recipients

18 The advantage of embryo transfer Disease control Breeding improvement

19 The advantage of cryopreservation of embryos * Embryos contain the complete genome * Enables breeding centers to carry a wider range of stocks * Save space and money e.g., transportation * Afford protection against loss e.g., fire, disease and other hazards * Preserves special genetic combinations, inbred strains and mutations * Research in animal genetics

20 The variable degrees of success in cryopreservation of embryos in different mammalian species Varied responses of certain stages of embryonic development to different biophysical and physiochemical parameters The nature of and concentration protocol of the cryopreservation used The type of programmable freezer The thawing rate The dilution protocol of the concentration of the cryoprotectant after thawing

21 Embryos damaged caused by The formation of large intracellular ice crystals The increased intracellular concentration of solutes and accompanying changes

22 Removing cytoplasmic lipids from early embryos Freezing of the hatching and hatched blastocysts Vitrification Cryopreservation of pig embryos Three approaches to the successful cryopreservation of pig embryos

23 In vitro production of pig embryos An insufficient cytoplasmic maturation of the oocyte An unusually high degree of polyspermic fertilizstion Low numbers of viable blastocysts Too few cells resulting in low development in vivo

24 Improvement of IVP of pig embryos 2-step protocol of IVM of pig oocytes Addition of glutathione is beneficial for the formation of male pronucleus Capacitated semen; epididymal semen after freezing and thawing Co-culture of oocytes with epithelial cells; addition of oviductal fluid; preincubation of sperm with FF to reduce the high proportion of polyspermy Addition of hyaluronic acid to increase monospermic fertilization in IVF

25 Generation of identical multiples Blastomeres from the 4- and 8-cell stage have potential to grow into blastocysts and to undergo in vivo development blastomere-derived blastocysts have lower ratio of ICM/TE than intact blastocysts Nuclear transfer produces larger numbers of genetically identical animals of a single genotype methods: electrofusion or microinjection donor cells: blastomere, blastocyst-derived cells, fetal fibroblast small fibroblasts with a smooth sufrace recipient oocytes: in vivo derived oocytes activation: Ca2+ dependent and Ca2+ independent approaches

26 The aims of transgenic technology in swine Enhance growth and development Increase disease resistance Produce foreign proteins in milk For xenotransplantation * similarity of anatomy and physiology to human organs * possible to be raised under the highest hygienic standards

27 The methods to produce transgenic animals Retroviral gene transfer methods Pronuclear injection of foreign DNA into fertilized ova Injection of pluripotent or totipotent embryonic cells Sperm-mediated exogenous DNA transfer during IVF Nuclear transfer with embryonic and adult somatic cells

28 Embryos or oocytes are exposed in vitro to concentrated virus solutions or incubated over a single layer of virus-producing cells. The virus enters the perivitelline space through a slit in the ZP The injection of virus under the ZP of oocytes with subsequent IVM and IVF.

29 The main advantage of retroviral-mediated gene transfer into animlas The technical ease The disadvantage of retroviral-mediated gene transfer into animlas the sequence of DNA transferred is limited by size the inserted gene is not always expressed in the second generation many founders are mosaic, with multiple insertion sites (breeding experiments required)

30 Pronuclear injection of DNA into a fertilized ovum

31 The primary advantage of microinjection of cloned DNA The higher efficiency compared to other methods The disadvantage of microinjection technique the method could not be used with embryos of later developmental stage (>4-cell stages) difficult to screen the insertion sites, multiple copies with configuration of tandem head-to-tail matter or array livestock species have a much lower frequency of intergatrion of foreign DNA into their chromsomes

32 The porcine ES cells (8-15 mm diameter) exhibit a high nuclear/cytoplasmic ratio and contain several prominent nucleioli. The ES cells usually grow in colonies with colony diameters ranging from mm.

33 Isolation of ES cells, transformation/transfection of ES cells and injection of ES cells into blastocysts to produce chimeric animals

34 The main advantages of using ES cells for production of transgenic animals ES cells may be grown in vitro for many generations ES cells could be transformed in vitro with foreign DNA ES cell line can be isolated and derived from a single cell transformed ES cells can be screened and selected for incorporation of the foreign DNA before injected ex., tissue specific expression offspring arisen from the ES cells carry the transgene in an identical site in the genome allow large numbers of genetically identical animals to be established efficiency of transgenic animal production may be increased it might be possible to replace existing livestock genes

35 The efficiency for production of ES cell lines from porcine blastocysts is low

36 Nuclear transfer with a karyoplast of a derived from blastomere from a preimplantation embryo or an adult somatic cell

Animal Fertilization Technologies

Animal Fertilization Technologies Appendix II-C Animal Fertilization Technologies Sperm storage The freezing of semen to 196 C, storage for an indefinite time, followed by thawing and successful insemination. Conception rates at first

More information

Lesson 3: Understanding Animal Reproduction Technology

Lesson 3: Understanding Animal Reproduction Technology Lesson 3: Understanding Animal Reproduction Technology 1 Terms Artificial insemination Cloning Donor female Embryo transfer Estrous synchronization Genetic engineering Progestin Prostaglandin Recipient

More information

ASA Presentation Notes Lesson 6.2

ASA Presentation Notes Lesson 6.2 ASA Presentation Notes Lesson 6.2 Unit Name: Unit 6 - Animal Reproduction Lesson Topic: Lesson 6.2 Generating Generations Presentation on: Livestock Breeding Systems Breeding Systems. Straight breeding.

More information

Abstracts for the KSAR and JSAR Joint Symposium. Fertility control in female domestic animals: From basic understanding to application

Abstracts for the KSAR and JSAR Joint Symposium. Fertility control in female domestic animals: From basic understanding to application Abstracts for the KSAR and JSAR Joint Symposium Fertility control in female domestic animals: From basic understanding to application Current Research Orientation in Livestock Reproduction in Korea Choong-Saeng

More information

TECH EXTENSION. How low can we go? Finding a practical path to produce more pigs from fewer and better boars.

TECH EXTENSION. How low can we go? Finding a practical path to produce more pigs from fewer and better boars. TECH EXTENSION How low can we go? Finding a practical path to produce more pigs from fewer and better boars www.fastgenetics.com Artificial insemination (AI) has become the standard breeding method for

More information

Evaluation and Economic Impact of Boar Fertility

Evaluation and Economic Impact of Boar Fertility Evaluation and Economic Impact of Boar Fertility A. Minton 1, A. Johnson 2, T. Werner 2, E. Triemert 2, N. Holden 2, G. Foxcroft 3, J. Patterson 3 and M. Dyck 3 1 PIC North America100 Bluegrass Commons

More information

Biotechnological Advances in Livestock Reproduction

Biotechnological Advances in Livestock Reproduction Biotechnological Advances in Livestock Reproduction By W. M. N. MWENYA*, OTHMAN ABAS MAZNI and HIROSHI KANAGAWA Faculty of Veterinary Medicine, Hokkaido University (Sapporo, Hokkaido, 060 Japan) Introduction

More information

Imagine Innovate Integrate. Using the NSET for embryo transfer and artificial insemination in mice and rats Kendra Steele, Ph.D.

Imagine Innovate Integrate. Using the NSET for embryo transfer and artificial insemination in mice and rats Kendra Steele, Ph.D. Imagine Innovate Integrate Using the NSET for embryo transfer and artificial insemination in mice and rats Kendra Steele, Ph.D. Outline- Here to make your life easier Non-surgical embryo transfer in mice

More information

Reproductive biotechnologies: current status in porcine reproduction

Reproductive biotechnologies: current status in porcine reproduction Ž. Animal Reproduction Science 60 61 2000 161 172 www.elsevier.comrlocateranireprosci Reproductive biotechnologies: current status in porcine reproduction B.N. Day ) Animal Science Unit, UniÕersity of

More information

but it still needs a bit of work

but it still needs a bit of work but it still needs a bit of work jc@embryos.net Reprogenetics ART Institute of Washington Life Global Principle investigator of cytoplasmic transfer series (1996-2001) Is there an alternative to MRT? Lessons

More information

Proceedings, Applied Reproductive Strategies in Beef Cattle December 2 and 3, 2008, Fort Collins, CO

Proceedings, Applied Reproductive Strategies in Beef Cattle December 2 and 3, 2008, Fort Collins, CO Proceedings, Applied Reproductive Strategies in Beef Cattle December 2 and 3, 2008, Fort Collins, CO NEW ADVANCEMENTS IN REPRODUCTIVE TECHNOLOGY FOR CATTLE George E. Seidel, Jr. Animal Reproduction and

More information

Proper steps for bull semen dilution and freezing. IMV Technologies France

Proper steps for bull semen dilution and freezing. IMV Technologies France Proper steps for bull semen dilution and freezing IMV Technologies France Introduction Since Polge reported the first successful cryopreservation of spermatozoa in 1949, spermatozoa from many mammalian

More information

1 st International Symposium on Bison Health

1 st International Symposium on Bison Health Faculty of Veterinary Medicine 1 st International Symposium on Bison Health Assisted Reproductive Technologies in Bison Robert McCorkell June 26, 2015 Artificial Insemination Frozen semen Estrus synchronization

More information

ONTARIO PORK RESEARCH PROPOSAL FINAL REPORT

ONTARIO PORK RESEARCH PROPOSAL FINAL REPORT PROPOSAL # 04/16 ONTARIO PORK RESEARCH PROPOSAL FINAL REPORT Project Leader: R.N. Kirkwood/ R.M. Friendship Project Title: Effect of addition of seminal plasma to thawed semen on sow fertility Objectives

More information

AI and its Influence on Production Efficiency

AI and its Influence on Production Efficiency AI and its Influence on Production Efficiency Christianne E. Glossop - Malmesbury, Wiltshire, England 2007 Introduction AI in pigs is by no means a new technique. Methods for semen collection and processing,

More information

by Bergero and Cynthia Préfontaine photos by Shary B. Akers Introduction

by Bergero and Cynthia Préfontaine photos by Shary B. Akers Introduction by Bergero and Cynthia Préfontaine photos by Shary B. Akers Introduction The embryo transfer (ET= embryo transfer) is a breeding method or reproductive technology, where an embryo is flushed from a donor

More information

Effects of Preservation of Porcine Oocytes by Dibutyryl Cyclic AMP on in vitro Maturation, Fertilization and Development

Effects of Preservation of Porcine Oocytes by Dibutyryl Cyclic AMP on in vitro Maturation, Fertilization and Development JARQ 45 (3), 295 300 (2011) http://www.jircas.affrc.go.jp of Porcine Oocytes Using dbcamp Effects of of Porcine Oocytes by Dibutyryl Cyclic AMP on in vitro Maturation, Fertilization and Development Dai-ichiro

More information

Cryopreservation of Porcine Gametes: A Chilly Future in the Swine Industry. EM Walters

Cryopreservation of Porcine Gametes: A Chilly Future in the Swine Industry. EM Walters Cryopreservation of Porcine Gametes: A Chilly Future in the Swine Industry EM Walters National Swine Resource and Research Center and Veterinary Pathobiology, University of Missouri, Columbia, MO Corresponding

More information

New Assisted Reproductive Techniques for Horses. Dirk K. Vanderwall, DVM, PhD, Dipl. ACT

New Assisted Reproductive Techniques for Horses. Dirk K. Vanderwall, DVM, PhD, Dipl. ACT New Assisted Reproductive Techniques for Horses Dirk K. Vanderwall, DVM, PhD, Dipl. ACT Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science Center for Reproductive Biology

More information

Embryo Transfer. Objectives. Performing Embryo Transfer. History of Embryo Transfer. Benefits for Embryo Transfer

Embryo Transfer. Objectives. Performing Embryo Transfer. History of Embryo Transfer. Benefits for Embryo Transfer 33 Embryo Transfer 305 Daniel Stein and Glenn Selk Objectives Discuss the history of embryo transfer. Learn the benefits of embryo transfer. Learn how to transfer embryos. Discuss the potential costs of

More information

In vitro production of equine embryos and genetic testing

In vitro production of equine embryos and genetic testing In Vitro Fertilization (FIV) In Vitro Production of Equine Embryos and Preimplantation Genetic Testing Oocytes from or slaughterhouse ovaries Sperm sample Carolina Herrera, M Sc. Clinic for Reproductive

More information

Tammie Roy Genea Biomedx Sydney, Australia. Declared to be stakeholder in Genea Biomedx

Tammie Roy Genea Biomedx Sydney, Australia. Declared to be stakeholder in Genea Biomedx Tammie Roy Genea Biomedx Sydney, Australia Declared to be stakeholder in Genea Biomedx 1 24-25 September 2015 Madrid and Alicante, Spain Importance of cryopreservation in Assisted Reproductive Technology

More information

Understanding eggs, sperm and embryos. Marta Jansa Perez Wolfson Fertility Centre

Understanding eggs, sperm and embryos. Marta Jansa Perez Wolfson Fertility Centre Understanding eggs, sperm and embryos Marta Jansa Perez Wolfson Fertility Centre What does embryology involve? Aims of the embryology laboratory Creation of a large number of embryos and supporting their

More information

EMBRYO TRANSFER ANIMAL SCIENCE 8818-B INTRODUCTION

EMBRYO TRANSFER ANIMAL SCIENCE 8818-B INTRODUCTION ANIMAL SCIENCE 8818-B EMBRYO TRANSFER INTRODUCTION Embryo transfer* is a process by which an embryo is collected from a donor female and then transferred into a recipient female where the embryo completes

More information

EGG BANKS program at clinique ovo

EGG BANKS program at clinique ovo EGG BANKS program at clinique ovo HISTORY The first pregnancy resulting from egg donation in an IVF cycle was in 1983 Egg donation plays an important role in assisted reproductive technologies since it

More information

Timing of A.I. Swine AI 9/6/12

Timing of A.I. Swine AI 9/6/12 Breeding Herd Education Series 20 Timely, relevant & convenient learning Thank you for participating in SowBridge 20. To start this presentation, advance one slide by pressing enter or the down or right

More information

Effects of Centrifugation and Lipid Removal on the Cryopreservation of in Vitro Produced Bovine Embryos at the Eight-Cell Stage

Effects of Centrifugation and Lipid Removal on the Cryopreservation of in Vitro Produced Bovine Embryos at the Eight-Cell Stage CRYOBIOLOGY 36, 206 212 (1998) ARTICLE NO. CY982077 Effects of Centrifugation and Lipid Removal on the Cryopreservation of in Vitro Produced Bovine Embryos at the Eight-Cell Stage M. Murakami,* T. Otoi,

More information

Unit B: Anatomy and Physiology of Poultry. Lesson 4: Artificial Poultry Reproduction

Unit B: Anatomy and Physiology of Poultry. Lesson 4: Artificial Poultry Reproduction Unit B: Anatomy and Physiology of Poultry Lesson 4: Artificial Poultry Reproduction 1 1 Terms Artificial insemination Sexed semen Standing heat 2 2 I. Artificial insemination is the placing of semen in

More information

DRB666 Applied Developmental and Reproductive Biology (Spring 2013)

DRB666 Applied Developmental and Reproductive Biology (Spring 2013) DRB666 Applied Developmental and Reproductive Biology (Spring 2013) Director: 651 Ilalo Street, BSB163-3 e-mail: yyamazak@hawaii.edu Phone: (808) 692-1416 Instructors (e-mail): Steve Ward Yusuke Marikawa

More information

Influence of Age, Body Weight and Body Condition on Plasma Leptin Concentrations in Beef Cattle Findings Introduction Materials and Methods

Influence of Age, Body Weight and Body Condition on Plasma Leptin Concentrations in Beef Cattle Findings Introduction Materials and Methods Influence of Age, Body Weight and Body Condition on Plasma Leptin Concentrations in Beef Cattle G.T. Gentry, Reproductive Biology Center, J.A. Roberts, L.R. Gentry and R.A. Godke, School of Animal Sciences

More information

Next Generation AI - New Developments to Maximize Efficiency Christianne E Glossop, Malmesbury, England

Next Generation AI - New Developments to Maximize Efficiency Christianne E Glossop, Malmesbury, England Next Generation AI - New Developments to Maximize Efficiency Christianne E Glossop, Malmesbury, England AI has been in use worldwide for almost 50 years. As it reaches maturity, the industry is demanding

More information

DEVELOPMENTS in reproduction continue

DEVELOPMENTS in reproduction continue Important Reproductive Technologies DEVELOPMENTS in reproduction continue to advance. Like humans, animals struggle with reproduction from time to time. Challenges facing the animal industry include the

More information

Division of Dairy Extension, National Dairy Research Institute, Karnal , India Received: Accepted: ABSTRACT

Division of Dairy Extension, National Dairy Research Institute, Karnal , India Received: Accepted: ABSTRACT Agri. Review, Vol.33, (2): 159-164, 2012 AGRICULTURAL RESEARCH COMMUNICATION CENTRE www.ar.arccjour ccjournals.com / indianjournals.com nals.com REPRODUCTIVE BIOTECHNOLOG OGY IN SMALL RUMINANTS - A REVIEW

More information

Manipulation of fertility to enhance productivity of cattle

Manipulation of fertility to enhance productivity of cattle Manipulation of fertility to enhance productivity of cattle Michael K. Holland and Michael McGowan (University of Queensland, Australia) Fertility is most simply defined as the natural capacity to produce

More information

Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 1

Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 1 AN163 Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 1 Gary R. Hansen 2 Introduction Through recent advances in reproductive technology, scientists have the ability to pre-select the

More information

Benefits of OPU/IVF (IVP) in Dairy Cattle. M.V. Ramon Tosta Duarte Deforest WI - Reproduction Supervisor ST-Genetics

Benefits of OPU/IVF (IVP) in Dairy Cattle. M.V. Ramon Tosta Duarte Deforest WI - Reproduction Supervisor ST-Genetics Benefits of OPU/IVF (IVP) in Dairy Cattle M.V. Ramon Tosta Duarte Deforest WI - Reproduction Supervisor ST-Genetics What is OPU/IVF (IVP)? ARTs used at ST Genetics Donor Selection Donors Animal Welfare

More information

Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes

Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes 1369 Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes C. S. Park*, M. Y. Kim, Y. J. Yi, Y. J. Chang, S. H. Lee 1, J. J. Lee 2, M. C. Kim 3 and D. I. Jin Division

More information

Study on Several Factors Involved in IVF-ET of Human Beings

Study on Several Factors Involved in IVF-ET of Human Beings Study on Several Factors Involved in IVF-ET of Human Beings Lei X 1, Zhuoran W 1, Bin L 1, Huiming L 1, Hongxiu Z 1, Yajuan Z 1, Yingbo Q 1, Guixue Z 2 1 The First Clinical College of Harbin Medical University,

More information

The French Cryobank of biological material as safe ex-situ conservation C. Danchin-Burge INRA - UMR GABI / Institut de l'elevage France

The French Cryobank of biological material as safe ex-situ conservation C. Danchin-Burge INRA - UMR GABI / Institut de l'elevage France The French Cryobank of biological material as safe ex-situ conservation C. Danchin-Burge INRA - UMR GABI / Institut de l'elevage France 1 20 breeds of horses and donkeys 10 breeds of goat France is the

More information

Reproductive Technology, Genetic Testing, and Gene Therapy

Reproductive Technology, Genetic Testing, and Gene Therapy Michael Cummings Chapter 16 Reproductive Technology, Genetic Testing, and Gene Therapy David Reisman University of South Carolina 16.1 Infertility Is a Common Problem In the US, about 13% of all couples

More information

Travel Award Chris Grupen to Attend the 9 th International Conference on Pig Reproduction & Visit INRA Poland/France

Travel Award Chris Grupen to Attend the 9 th International Conference on Pig Reproduction & Visit INRA Poland/France Travel Award Chris Grupen to Attend the 9 th International Conference on Pig Reproduction & Visit INRA Poland/France Final Report APL Project 2012/2429 August 2013 The University of Sydney Dr Christopher

More information

Current Status and Perspectives in Reproductive Biology and Biotechnology

Current Status and Perspectives in Reproductive Biology and Biotechnology [Satellite Symposium of the JSAR Annual Meeting 2004] Current Status and Perspectives in Reproductive Biology and Biotechnology Abstracts for Section 3: The 9th Asian Symposium on Animal Biotechnology

More information

UNIVERSITY OF WASHINGTON MEDICAL CENTER Men s Health Center and Male Fertility Laboratory Sperm & Testis Cryopreservation Program Patient NAME and ID

UNIVERSITY OF WASHINGTON MEDICAL CENTER Men s Health Center and Male Fertility Laboratory Sperm & Testis Cryopreservation Program Patient NAME and ID UNIVERSITY OF WASHINGTON MEDICAL CENTER Men s Health Center and Male Fertility Laboratory Sperm & Testis Cryopreservation Program Patient NAME and ID CONSENT FOR MINOR s SPERM OR TESTIS CRYOPRESERVATION

More information

Unit B Understanding Animal Body Systems. Lesson 7 Understanding Animal Reproduction

Unit B Understanding Animal Body Systems. Lesson 7 Understanding Animal Reproduction Unit B Understanding Animal Body Systems Lesson 7 Understanding Animal Reproduction 1 Terms Anestrus Artificial insemination Castration Cervix Copulation Diestrus Egg Ejaculation Estrous cycle Estrus Fertilization

More information

Artificial Insemination in Swine

Artificial Insemination in Swine Artificial Insemination in Swine Artificial Insemination in Swine Routinely practiced on swine farms More efficient and effective use of boars AI centers distribute semen from genetically valuable boars

More information

SYMPOSIUM: REPRODUCTIVE TECHNOLOGY AND GENETIC IMPROVEMENT

SYMPOSIUM: REPRODUCTIVE TECHNOLOGY AND GENETIC IMPROVEMENT SYMPOSIUM: REPRODUCTIVE TECHNOLOGY AND GENETIC IMPROVEMENT Current Status and Potential of Embryo Transfer and Reproductive Technology in Dairy Cattle ABSTRACT Significant use of embryo transfer in dairy

More information

Male Fertility: Your Questions Answered

Male Fertility: Your Questions Answered Male Fertility: Your Questions Answered Michael S. Neal Scientific Director, ONE Fertility, 3210 Harvester Rd. Burlington, Ontario www.onefertility.com mneal@onefertility.com Outline Assisted Conception

More information

DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2011

DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2011 DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2011 Director: 651 Ilalo Street, BSB163-3 e-mail: yyamazak@hawaii.edu Phone: (808) 692-1416 Instructors (e-mail): Steve Ward Yusuke

More information

CLINICAL RESULTS 2017.

CLINICAL RESULTS 2017. CLINICAL RESULTS 2017. VERIFICATION REPORT RESULTS OF ASSISTED REPRODUCTION TREATMENTS PERFORMED AT IVI GROUP IN 2017. In Vitro Fertilisation (IVF-ICSI) Egg donation (OVODON) Pre-Implantation Genetic Diagnosis

More information

Fertility of a Single Service: Annual Cost of Early Embryonic Loss to U.S. Beef Industry. Nutrient Partitioning

Fertility of a Single Service: Annual Cost of Early Embryonic Loss to U.S. Beef Industry. Nutrient Partitioning Fertility in Beef Cows Tom Geary Reproductive Physiologist Fertility of a Single Service: Beef Cattle Early Embryonic Loss 95 100 Percentage, % 80 Maternal Recognition of Pregnancy Failure - most loss

More information

CARD HyperOva (Superovulation Reagent for mouse)

CARD HyperOva (Superovulation Reagent for mouse) Product manual (Superovulation Reagent for mouse) Cat. No. KYD-010-EX -X5 Size: 5 1 ML Origin Serum of goat, Horse-derived villus gonatropin. Composition 1. Inhibin antiserum (Goat). 2. Equine chorionic

More information

The storage of cow eggs at room temperature and at low temperatures

The storage of cow eggs at room temperature and at low temperatures The storage of cow eggs at room temperature and at low temperatures A. O. Trounson, S. M. Willadsen, L. E. A. Rowson and R. Newcomb A.R.C. Unit of Reproductive Physiology and Biochemistry, Cambridge, U.K.*

More information

Rejuvenation of Gamete Cells; Past, Present and Future

Rejuvenation of Gamete Cells; Past, Present and Future Rejuvenation of Gamete Cells; Past, Present and Future Denny Sakkas PhD Scientific Director, Boston IVF Waltham, MA, USA Conflict of Interest I have no conflict of interest related to this presentation.

More information

UNDERSTANDING EMBRYO-TRANSFER (ET) A GUIDE TO THE BENEFIT OF ET IN YOUR HERD

UNDERSTANDING EMBRYO-TRANSFER (ET) A GUIDE TO THE BENEFIT OF ET IN YOUR HERD UNDERSTANDING EMBRYO-TRANSFER (ET) A GUIDE TO THE BENEFIT OF ET IN YOUR HERD Embryo Transfer allows one superior cow to produce a greater number of calves than normal in her lifetime TABLE OF CONTENTS

More information

We can give you the very best chance of having a baby.

We can give you the very best chance of having a baby. We can give you the very best chance of having a baby www.ivf-iscare.com ISCARE was founded in 1994 as a first private clinic specialized in reproductive medicine in the Czech Republic. This centre of

More information

RapiDVIT & rapidwarm oocyte. Specialised media for oocyte vitrification.

RapiDVIT & rapidwarm oocyte. Specialised media for oocyte vitrification. RapiDVIT & rapidwarm oocyte Specialised media for oocyte vitrification. Special media for A unique cell Cryopreservation of oocytes requires care. Some preservation techniques cause premature oocyte activation

More information

II. The effect of strain and IGF-1 gene transfer using SMGT techniques on some semen characteristics:-

II. The effect of strain and IGF-1 gene transfer using SMGT techniques on some semen characteristics:- The experimental work of this study was carried out in poultry farm of the Department of Animal Production, Faculty of Agriculture, Benha University, Egypt with cooperation of Genetic Engineering and Biotechnology

More information

Comparison between Low/Programmable Freezing and Fast Freezing Protocols of Hungarian Guinea Fowl Semen

Comparison between Low/Programmable Freezing and Fast Freezing Protocols of Hungarian Guinea Fowl Semen Athens Journal of Natural & Formal Sciences September 2014 Comparison between Low/Programmable Freezing and Fast Freezing Protocols of Hungarian Guinea Fowl Semen By Thieu Ngoc Lan Phuong Eva Varadi Barbara

More information

Draft. Draft. 2. The system of breeding which breeds a registered male to a registered female animal of the same breed is:

Draft. Draft. 2. The system of breeding which breeds a registered male to a registered female animal of the same breed is: Student Name: Draft Teacher: Date: District: Wake County Assessment: 9_12 Agriculture AA21 - Animal Science I Test 4 Description: Test 7: Reproduction & Genetics Form: 501 Draft 1. Superior traits of offspring

More information

Past, present and future of bovine in vitro embryo production in Denmark. Poul Hyttel and Lotte Strøbech University of Copenhagen

Past, present and future of bovine in vitro embryo production in Denmark. Poul Hyttel and Lotte Strøbech University of Copenhagen Past, present and future of bovine in vitro embryo production in Denmark Poul Hyttel and Lotte Strøbech University of Copenhagen Bovine in vitro embryo production in DK - looking back Royal Veterinary

More information

GEMouse. Added Service Guide

GEMouse. Added Service Guide Contact mouse : mouse@macrogen.com Payment Inquiry: payment@macrogen.com Technical Support Macrogen Korea: support@macrogen.com Macrogen-Europe: support-europe@macrogen.com Update 68 GEMouse Added Service

More information

CONSERVATION OF ANCIENT BREED SMALL RUMINANTS AS FROZEN EMBRYOS

CONSERVATION OF ANCIENT BREED SMALL RUMINANTS AS FROZEN EMBRYOS Bulgarian Journal of Veterinary Medicine (2008), 11, No 4, 251 255 CONSERVATION OF ANCIENT BREED SMALL RUMINANTS AS FROZEN EMBRYOS Summary E. SAPUNDZHIEV Faculty of Veterinary Medicine, University of Forestry,

More information

Proceedings of the Society for Theriogenology 2013 Annual Conference

Proceedings of the Society for Theriogenology 2013 Annual Conference www.ivis.org Proceedings of the Society for Theriogenology 2013 Annual Conference Aug. 7-10, 2013 Louisville, KY, USA www.therio.org/ Next SFT Meeting: Aug. 6-9, 2014 Portland, OR, USA Reprinted in the

More information

Preimplantation genetic diagnosis: polar body and embryo biopsy

Preimplantation genetic diagnosis: polar body and embryo biopsy Human Reproduction, Vol. 15, (Suppl. 4), pp. 69-75, 2000 Preimplantation genetic diagnosis: polar body and embryo biopsy Luca Gianaroli SISMER, Via Mazzini 12, 40138 Bologna, Italy Scientific Director

More information

DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2018

DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2018 DRB666 Applied Developmental and Reproductive Biology Spring Semester, 2018 Director:, DVM, Ph.D. 651 Ilalo Street, BSB163-3 e-mail: yyamazak@hawaii.edu Phone: (808) 692-1416 Instructors (e-mail): Steve

More information

OVERVIEW AND FACTS: CRYOPRESERVATION

OVERVIEW AND FACTS: CRYOPRESERVATION OVERVIEW AND FACTS: CRYOPRESERVATION Imprint Published in August, 2013 By Victory A.R.T. Laboratory Phils, Inc. This ebook was created by http://www.ivfvictoryphilippines.com/ in hopes of helping bring

More information

Adoption and Foster Care

Adoption and Foster Care GLOSSARY Family building via Adoption and Foster Care October 2018 www.familyequality.org/resources A Anonymous Donor: A person who donated sperm or eggs with the intention of never meeting resulting children.

More information

Handmade cloning: an alternative technique for somatic cell nuclear transfer. Esmaeilzadeh Mahdi and Hosseini Fakhrisadat

Handmade cloning: an alternative technique for somatic cell nuclear transfer. Esmaeilzadeh Mahdi and Hosseini Fakhrisadat Available online at www.scholarsresearchlibrary.com Annals of Biological Research, 2012, 3 (6):3043-3048 (http://scholarsresearchlibrary.com/archive.html) ISSN 0976-1233 CODEN (USA): ABRNBW Handmade cloning:

More information

Reproductive Techniques

Reproductive Techniques Reproductive Techniques Policy Number: 4.02.04 Last Review: 12/2017 Origination: 10/1988 Next Review: 12/2018 Policy Blue Cross and Blue Shield of Kansas City (Blue KC) will provide coverage for assisted

More information

RESEARCHES REGARDING BOAR SEMEN CRYOPRESERVATION

RESEARCHES REGARDING BOAR SEMEN CRYOPRESERVATION UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINRY MEDICINE CLUJ-NAPOCA DOCTORAL SCHOOL Faculty of Veterinary Medicine SOTOC OCTAV CORNELIU SORIN SUMMARY OF THE PHD THESIS RESEARCHES REGARDING BOAR SEMEN

More information

Title. Author(s)TEKELI, Tevfik; KWEON, Oh Kyeong; KANAGAWA, Hiroshi. CitationJapanese Journal of Veterinary Research, 35(4): 283-

Title. Author(s)TEKELI, Tevfik; KWEON, Oh Kyeong; KANAGAWA, Hiroshi. CitationJapanese Journal of Veterinary Research, 35(4): 283- Title THE VIABILITY OF DEEP-FROZEN AGGREGATED MOUSE EMBRYO Author(s)TEKELI, Tevfik; KWEON, Oh Kyeong; KANAGAWA, Hiroshi CitationJapanese Journal of Veterinary Research, 35(4): 283- Issue Date 1987-10-30

More information

CONSENT TO CRYOPRESERVATION AND STORAGE OF HUMAN EMBRYOS

CONSENT TO CRYOPRESERVATION AND STORAGE OF HUMAN EMBRYOS 1. Name(s) of Party/Parties A. Party/parties requesting freezing of embryos a. Couple We, and of County, City of in the state of are (married or domestic partners) and are over the age of twenty-one years.

More information

Effect of Frequency of Boar Exposure on Estrus and Ovulation in Weaned Sows as Determined by Real-Time Ultrasound.

Effect of Frequency of Boar Exposure on Estrus and Ovulation in Weaned Sows as Determined by Real-Time Ultrasound. Effect of Frequency of Boar Exposure on Estrus and Ovulation in Weaned Sows as Determined by Real-Time Ultrasound. Robert V. Knox, Kilby Willenburg, Gina Miller, Sandra Rodriguez-Zas Department of Animal

More information

1- THE USE OF EARLY-AGE FEED RESTRICTION AND/OR POTASSIUM CHLORIDE FOR ALLEVIATING THE ADVERSE EFFECTS OF HEAT STRESS ON BROILER CHICKS: 1.

1- THE USE OF EARLY-AGE FEED RESTRICTION AND/OR POTASSIUM CHLORIDE FOR ALLEVIATING THE ADVERSE EFFECTS OF HEAT STRESS ON BROILER CHICKS: 1. 1- THE USE OF EARLY-AGE FEED RESTRICTION AND/OR POTASSIUM CHLORIDE FOR ALLEVIATING THE ADVERSE EFFECTS OF HEAT STRESS ON BROILER CHICKS: 1. EFFECTS ON BROILER PERFORMANCE, CARCASS TRAITS AND ECONOMIC EFFICIENCY.

More information

FERTILIZATION AND EMBRYONIC DEVELOPMENT IN VITRO

FERTILIZATION AND EMBRYONIC DEVELOPMENT IN VITRO FERTILIZATION AND EMBRYONIC DEVELOPMENT IN VITRO FERTILIZATION AND EMBRYONIC DEVELOPMENT IN VITRO Edited by Luigi Mastroianni, Jr. University of Pennsylvania Philadelphia, Pennsylvania and John D. Biggers

More information

DEVELOPMENT OF A COMMERCIAL EMBRYO

DEVELOPMENT OF A COMMERCIAL EMBRYO DEVELOPMENT OF A COMMERCIAL EMBRYO FREEZING PROTOCOL FOR THE CONSERVATION, TRANSPORTATION AND IMPORTATION OF PIG GENETICS 2E-105 Report prepared for the Co-operative Research Centre for an Internationally

More information

EMBRYO TRANSFER IN HORSES

EMBRYO TRANSFER IN HORSES Vet Times The website for the veterinary profession https://www.vettimes.co.uk EMBRYO TRANSFER IN HORSES Author : Jonathan F Pycock, James R Crabtree Categories : Vets Date : March 14, 2011 Jonathan F

More information

Effect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes

Effect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes Theriogenology Insight: 4(3): 17-111, December, 214 DOI Number: 1.98/2277-3371.214.74.2 Effect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes K M

More information

Female Patient Name: Social Security # Male Patient Name: Social Security #

Female Patient Name: Social Security # Male Patient Name: Social Security # Female Patient Name: Social Security # Male Patient Name: Social Security # THE CENTER FOR HUMAN REPRODUCTION (CHR) ILLINOIS/NEW YORK CITY * ASSISTED REPRODUCTIVE TECHNOLOGIES PROGRAM (A.R.T.) CRYOPRESERVATION

More information

EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM

EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM COLLEGE OF VETERINARY MEDICINE & BIOMEDICAL SCIENCES Department of Large Animal Clinical Sciences 4475 TAMU EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM Section of Theriogenology Veterinary Medical

More information

The Influence of Developmental Stage and Morphological Quality of Frozen-Thawed Bovine Embryos on Pregnancy Rate in Bovine Embryo Transfer

The Influence of Developmental Stage and Morphological Quality of Frozen-Thawed Bovine Embryos on Pregnancy Rate in Bovine Embryo Transfer Journal of Reproduction and Development, Vol. 45, No. 4, 1999 Technical Note The Influence of Developmental Stage and Morphological Quality of Frozen-Thawed Bovine Embryos on Pregnancy Rate in Bovine Embryo

More information

Future of Mouse Embryo Transfer: Achieving the 3Rs with the NSET device

Future of Mouse Embryo Transfer: Achieving the 3Rs with the NSET device Future of Mouse Embryo Transfer: Achieving the 3Rs with the NSET device Presented by Barbara Stone, PhD ParaTechs Corporation Lexington, KY, USA What is Embryo Transfer? Removal of viable embryos from

More information

Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs 1

Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs 1 Fertilization and blastocyst development in oocytes obtained from prepubertal and adult pigs 1 E. S. Sherrer 2, T. J. Rathbun, and D. L. Davis 3 *Department of Animal Sciences and Industry, Kansas State

More information

Breeding & Genetic Engineering. Laura Garzel, DVM

Breeding & Genetic Engineering. Laura Garzel, DVM Breeding & Genetic Engineering Laura Garzel, DVM Part I GENETICS Genetics Definition: the biological science dealing with heredity Gain an understanding of why offspring have similarities and differences

More information

The IVF Bioscience Media Brochure

The IVF Bioscience Media Brochure The IVF Bioscience Media Brochure IVF Bioscience Visit us at ivfbioscience.com to find out more IVF Bioscience manufactures high quality, species specific media for in vitro fertilisation (IVF). Our innovative

More information

Preservation of Liquid Boar Semen: Effect of Genotype, Boar and Sperm Parameters on Motility and Acrosome Integrity

Preservation of Liquid Boar Semen: Effect of Genotype, Boar and Sperm Parameters on Motility and Acrosome Integrity VETERINARY RESEARCH INTERNATIONAL Journal homepage: www.jakraya.com/journal/vri ORIGINAL ARTICLE Preservation of Liquid Boar Semen: Effect of Genotype, Boar and Sperm Parameters on Motility and Acrosome

More information

Biology 4361 Developmental Biology. October 11, Multiple choice (one point each)

Biology 4361 Developmental Biology. October 11, Multiple choice (one point each) Biology 4361 Developmental Biology Exam 1 October 11, 2005 Name: ID#: Multiple choice (one point each) 1. Sertoli cells a. surround spermatocytes b. are the structural components of the seminiferous tubules

More information

GYNEM PRICELIST 2018

GYNEM PRICELIST 2018 EGG DONATION 5 300 EUR - One donor to one recipient - 6 oocytes minimum guarantee (average 8-12 oocytes) - Initial consultation, compulsory pre-treatment tests - Standard stimulation medications for the

More information

Infertility treatment

Infertility treatment In the name of God Infertility treatment Treatment options The optimal treatment is one that provide an acceptable success rate, has minimal risk and is costeffective. The treatment options are: 1- Ovulation

More information

Fertilization depends on mechanisms that help sperm meet eggs of the same species.

Fertilization depends on mechanisms that help sperm meet eggs of the same species. Fertilization depends on mechanisms that help sperm meet eggs of the same species. www.uchsc.edu/ltc/fertilization.html Fertilization union of sperm and egg Is a chain of events. Interruption of any step

More information

EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM

EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM COLLEGE OF VETERINARY MEDICINE & BIOMEDICAL SCIENCES Department of Large Animal Clinical Sciences 4475 TAMU January 2016 b EQUINE INTRACYTOPLASMIC SPERM INJECTION PROGRAM Section of Theriogenology & the

More information

In vitro Culture, Storage and Transfer of Goat Embryos

In vitro Culture, Storage and Transfer of Goat Embryos Aust. J. Bio!. Sci., 1976,29, 125-9 In vitro Culture, Storage and Transfer of Goat Embryos R. J. Bilton and N. W. Moore Department of Animal Husbandry, University of Sydney, Camden, N.S.W. 2570. Abstract

More information

Effect of Bovine Follicular Fluid Added to the Maturation Medium on Sperm Penetration in Pig Oocytes Matured In Vitro

Effect of Bovine Follicular Fluid Added to the Maturation Medium on Sperm Penetration in Pig Oocytes Matured In Vitro Article Effect of Bovine Follicular Fluid Added to the Maturation Medium on Sperm Penetration in Pig Oocytes Matured In Vitro Abstract Naoki ISOBE Research Associate Graduate School for International Development

More information

N R C Mithun AUGMENTING REPRODUCTIVE EFFICIENCIES IN MITHUN THROUGH BIOTECHNOLOGICAL INTERVENTION. K.K.Baruah and M. Mondal

N R C Mithun AUGMENTING REPRODUCTIVE EFFICIENCIES IN MITHUN THROUGH BIOTECHNOLOGICAL INTERVENTION. K.K.Baruah and M. Mondal AUGMENTING REPRODUCTIVE EFFICIENCIES IN MITHUN THROUGH BIOTECHNOLOGICAL INTERVENTION K.K.Baruah and M. Mondal National Research Centre on Mithun Jharnapani, Medziphema, Nagaland 797 106, India Background

More information

EMMA Cryopreservation Workshop Novel concepts in mouse production and preservation

EMMA Cryopreservation Workshop Novel concepts in mouse production and preservation EMMA Cryopreservation Workshop Novel concepts in mouse production and preservation Consejo Superior de Investigaciones Cientificas Madrid, Espagna May 7-8, 2012 K. C. Kent Lloyd, DVM, PhD University of

More information

Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization

Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization W. H. Wang, L. R. Abeydeera, T. C. Cantley and B. N. Day Department of Animal Sciences, University of

More information

CLINICAL RESULTS 2016

CLINICAL RESULTS 2016 CLINICAL RESULTS 2016 RESULTS OF ASSISTED REPRODUCTIVE TREATMENTS CARRIED OUT ATTHEIVIGROUPDURING2016 In Vitro Fertilisation (IVF-ICSI) Ovum Donation (OVODON) Frozen Embryo Transfer PreimplantationGeneticDiagnosis

More information

Sorting semen may be advantageous The perspectives of sexed bull semen are wide and obvious for cattle breeding:

Sorting semen may be advantageous The perspectives of sexed bull semen are wide and obvious for cattle breeding: Sexed semen is a dream coming through Head of Research and Development Søren Borchersen, A.I. Centre Dansire E-mail-address: SOB@dansire.dk Bilag til konference i Dnepropetrovsk The use of sexed semen

More information

Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes

Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes Journal of Reproduction and Development, Vol. 46, No. 5, 2000 Original Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes Hiroaki FUNAHASHI

More information

IVF MONEY-BACK PLAN. More information about payment options? IN PARTNERSHIP WITH. Call Access Fertility or visit their website

IVF MONEY-BACK PLAN. More information about payment options? IN PARTNERSHIP WITH. Call Access Fertility or visit their website IVF MONEY-BACK PLAN More information about payment options? Call Access Fertility or visit their website IN PARTNERSHIP WITH Programmes Refund Programme Up to 3 fresh cycles of IVF/ICSI and all frozen

More information