MicroRNA. , microrna(mirna),, mirna mirna, mirna, mirna, mirna. : ; (NOA); microrna (mirna); : R698.2 : A : X(2014)

Transcription

1 34 11 Vol.34 No Nov Reproduction & Contraception doi: /j.issn X MicroRNA ( ) microrna() : ; (NOA); microrna (); : R698.2 : A : X(2014) %~ 15% 50% [2] (non-obstructive azoospermia NOA) (obstructive azoospermia OA) [3] OA (intracytoplasmic sperm injection ICSI) NOA : : ; Tel: ; caoyunxia6@126.com [1] Y 60%~70% NOA 3 : (hypo-spermato genesis) [Sertoli-cell-only (SCO) syndrome] (spermatogenesis arrest) (maturation arrest MA) [4] mrna Tnp1 Tnp2 Prm1 Prm2 mrna [56] Prm1 [7] Prm1 3' (3'UTR) Prm1 mrna 3'UTR MicroRNA () 22 RNA Lee RNA RNA 925

2 (pri-) [8] RNA Drosha-DGCR8 RNA(pre-) 60~80 nt [9] exportin5- RanGTP [10] RNA Dicer [11] RNA (RNA-induced silencing complex RISC) [12] RISC mrna [13] 30% mrna RNA 3'UTR ( ) RISC RNA RNA / RNA [14] [15-20] s s DGCR8 Drosha Dicer [15-17] 3 ; s 3'UTR [1819] 5'UTR ; s [20] [21] Yu [22] Tnp2 mrna 3'UTR mir-122 s 1 MiRNA 1.1 Yu [22] mir-122a mir-16 mir-469 mir-34 Yan [23] Yan [24] Luo [25] [26] mir ; mir-203 mir-34b-5p [27] Novotny [28] mir-17-5p E2F1(E2F transcription factor 1) ; mir-34c TGIF2 NOTCH2 [29] ; mir-18 2(heat shock factor 2 HSF2) [30] Lian MA [3132] mircurytmlnas 19 s 154 s mir-383 qrt-pcr mir-383 Lian [3132] 1(interferon regulatory factor IRF1) mir-383 mir- 383 IRF1 IFR1 IFR1 mir- 383 IFR1 mir-383 [3334] mir-383 G1/S transition cyclin D1-CDK4-p21 CDK2 G1/S prb mir- 383-pRb Park [35] mir-383

3 X (fragile X ental retardation protein FMRP) FMRP mir- 383 IFR1 mir- 383 IFR1 FMRP mir-383 s 3 : ; ; [36-38] DNA RNA [39] [4041] (cellfree cfs-) cfs- ( ) Weber 12 cfs- [40] 12 cfs- cfs- Huang RNA [39] 1.75 mg/l (<0.1 mg/l) cfs-rna 2.40~7.28 g (Microarray) Northern (Northern Blot) - (RT- PCR) RNA RNA RNA 28S rrna 18S rrna RNA ( 5S rrna trna microrna ) Wang 4 0~7 d 8 Li [42] 20% Wang ( NOA ) s(seminal plasma s sps) 19 s 7 s(mir-34c- 5p mir-122 mir-146b-5p mir-181a mir- 374b mir-509-5p mir-513a-5p) 7 sp-s ROC sps cfs-s Wu TaqMan qrt-pcr NOA sp-s [43] 3 sp-s(mir-141 mir-429 mir-7-1-3p) NOA sp- 3 sp-s(mir- 141 mir-429 mir-7-1-3p) 24 h Wu NOA sp-s [38] mir-19b let-7a sp- 4 RNA 927

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