InterMedics Training Academy (ITA) Embryology Training and Lab Management

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1 InterMedics Training Academy (ITA) Embrylgy Training and Lab Management Dr. James Catt (BSC Hns, PHD) Directr, ITA Frmer Scientific Directr, Sydney IVF, Mnash IVF InterMedics is at the frefrnt f IVF service delivery in India and has recently pened its wn training facility in Mumbai. Optimal IVF is an internatinal cnsultatin cmpany prviding specialist training in all IVF and related prcedures, and services in ptimizatin f IVF lab prtcls. These tw frces have nw cmbined tgether and are pleased and excited t ffer a series f shrt curses in embrylgy. Emphasis n hands n training Advanced statistical analysis f data t imprve utcmes Train n the latest equipment available in the field f ART Cnveniently lcated 20 mins frm the airprt and majr railway statins in Mumbai 204/205 Simran Plaza, 4th Rad, Khar West, Mumbai Tel: Fax: inf@intmedics.cm

2 Training Curses Intrductin t Embrylgy Curse Objective: Learn the principles f embrylgy and hw t run a successful labratry ICSI - Getting the Basics Right Curse Objective: Learn the imprtant things abut ICSI and start n the rad t cmpetency Vitrificatin and Laser Assisted Hatching Curse Objectives: Learn the practicalities f vitrificatin and laser hatching t be cmpetent fr clinical applicatin Quality Assurance and Quality Cntrl Curse Objective: Cntrl yur IVF prgramme. Learn QC/QA methdlgy and applicatins TESE and Advanced ICSI Learn the tricks f the trade when dealing with difficult cases Curse Objective: Learn hw t extract sperm frm testicular tissue and use it prperly in difficult ICSI cases

3 Intrductin t Embrylgy Duratin: 1 day The basics Ogenesis and vulatin Spermigenesis Prerequisites fr fertilisatin Ocyte maturatin Sperm capacitatin and acrsme reactin Nrmal / IVF fertilisatin ICSI fertilisatin Abnrmal fertilisatin Early cleavage Embrynic develpment The imprtant things Embry culture media, gas, dishes Embry handling pen, clsed, devices Crypreservatin vital part f IVF Expectatins frm an IVF clinic Review, Q & A

4 ICSI - getting the basics right Duratin: 3 Days ICSI is a technique that has been in use fr the past 18 years and has becme a mainstay f human IVF. ICSI is relatively straightfrward when cnducted prperly but can be difficult when the apparatus is nt set ptimally. This 3 day curse will take yu thrugh the principles f micrmanipulatin, utlining the differences between the micrmanipulatrs and the ptical systems used. The imprtance f gd quality pipettes will be emphasised alng with the critical areas f micr tl set up and cntrl f pressures. A series f exercises will be given t all participants t help them develp and maintain cmpetency. ICSI Day 1 Shrt series f lectures intrducing ICSI and the critical pints f micrmanipulatin Practical wrk will centre f setting up the relief cntrast system (RCM) and the starting set up f Narishige manipulatrs (carse mtr drive and hydraulic fine manipulatin). The crrect set up and rientatin f pipettes will be demnstrated alng with prtcls t ensure crrect alignment and cntrl. Afternn Sessin ICSI decnstructed Brief intrductin t the methdlgy and exercises Injectin pipette set up and sperm immbilisatin Sperm aspiratin and cntrl Ocyte alignment and mvement Sperm injectin Practice and sperm injectin Review, Q and A

5 ICSI - Day 2 Intrductin t cyte and dish preparatin. What is imprtant? Practical denuding f cytes with hyalurnidase. Injectin dish preparatin. Cntrl f cnditins Injectin practice Injectin practice, under supervisin Review, Q and A ICSI - Day 3 Talk n utcme expectatins. What t mnitr? Injectin practice Practical trubleshting. Defining the symptms Applying the crrectins. A series f the mst cmmn prblems alng with the fixes will be demnstrated. Injectin practice Review, summary Q and A

6 Vitrificatin and laser assisted hatching - 1 day Intrductin Principles f vitrificatin chice f media carriers and strage Practical vitrificatin f embrys Quality Assurance and Quality Cntrl Overview(QAQC) Intrductin Principles and methdlgy f assisted hatching Practical assisted hatching using embrys

7 Quality Assurance and Quality Cntrl 1 day This is a part taught, wrked example and practical demnstratin curse t lead yu thrugh the principles, lgistics and practicalities f hw a QA/QC prgramme shuld wrk t enable yu t cntrl, mnitr and cmpare yur prgramme Talk n principles f QA/QC Quality Cntrl f IVF instrumentatin and envirnment Independent calibratin f incubatrs, warm stages etc What is crrect? Demnstratin f mnitring equipment Envirnment Cntrl Air purity what are particles, VOC, CACS and what d we need t d abut them? Temperature and ph Instrument quality recrds design and use Quality cntrl f the IVF prcess Hw gd are we? What are the best utcme measures? Cncept and practicalities f KPI (key perfrmance indicatrs) and LPM (labratry perfrmance measures) Cntrl charting fr cntinual measurement and the use f CUSUM charts Practical demnstratin n hw t plt and interpret them Trubleshting Methdlgy and practical examples Benchmarking Intra and inter labratry benchmarking. Hw gd are we cmpared t thers? Review, Q and A

8 TESE and advanced ICSI 1 day Talk n principles and methdlgy Practical sessin Tubule recvery Open bipsy Fine needle aspiratin Sperm recvery frm tubules 'milking' 'squashing' 'maceratin' Sperm preparatin red cell lysis gradients 'swim ut' 'fall ut' Intrductin t advanced ICSI 'Rescue' ICSI 4 hur and 14 hur Practical sessin Immtile sperm hyp-smtic swelling (HOS) test Pentxifylline Calcium activatin Glbspermia Previus failed t fertilise ICSI cycles Immature cytes Pstmature cytes Truble shting talk Discussin n spindle view, IMSI and PICSI

InterMedics Training Academy (ITA) Embryology Training and Lab Management

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