Senior of Histopathology Department at Khartoum, Radiation and Isotopes Center
|
|
- Amos Hudson
- 6 years ago
- Views:
Transcription
1 EUROPEAN ACADEMIC RESEARCH Vol. IV, Issue 2/ May 2016 ISSN Impact Factor: (UIF) DRJI Value: 5.9 (B+) Immune Histochemical Evaluation of AMACR (P504S) in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia in Small Neddle Biopsy of Sudanese EL TAYEB EL FAITH ELTAYEB MSc Student in Medical Laboratory Sciences AL-Neelain University, Sudan EL SADIG A ADAM Department of Patholothology Al-Ribat National University, Sudan NADA SALIH SALIH Senior of Histopathology Department at Khartoum, Radiation and Isotopes Center Abstract: Background: Prostate cancer is a major health problem throughout the world. Immunohistochemistry plays a very important role in the diagnosis of minimal prostatic adenocarcinoma and to exclude one of its benign mimickers, but it should always be interpreted in the context of the H&E appearances. In some cases of minimal prostate cancer morphologic features do not allow a diagnosis of carcinoma. Methods: 50 needle biopsy specimens, including 28 with small biopsy of prostatic adenocarcinoma and 22 benign prostate were stained immunohistochemically with AMACR. Results: Of 28 cases of small foci of prostatic adenocarcinoma, 26 (92.9%) expressed AMACR; There was focal positive staining with AMACR in 2 benign cases. 1380
2 Conclusions: Immunostaining with AMACR (p504s) could improve the diagnostic performance and help in avoid carrying out new biopsies in small foci of prostatic carcinoma detection. Key words: Prostate cancer, AMACR (P504S) marker, needle biopsy 1. INTRODUCTION Worldwide, prostate cancer is the second most common malignancy in men after lung cancer [1] Diagnosis of prostate cancer glands can sometimes present a diagnostic challenge for pathologists, since prostate carcinoma can mimic benign prostate glands [2]. The diagnosis of prostatic adenocarcinoma, especially in needle biopsy samples, can occasionally be challenging, either because they only show small foci of prostatic adenocarcinoma, or because of the difficulty in distinguishing prostatic carcinoma from benign mimickers[3] The difficulty in the diagnosis of prostatic adenocarcinoma is mostly seen with minimal (limited<1mm) carcinoma in needle tissue [4] Many major and minor histologic features important for the diagnosis of minimal prostatic carcinoma should be assessed specifically at low- and highpower magnification. The first of the major criteria is an infiltrative growth pattern which frequently presents as the presence of small malignant glands between larger, more complex (and often paler), benign glands. [5] Alpha-methylacyl- CoA racemase (AMACR), formerly known as P504s, is a mitochondrial and peroxisomal enzyme involved in the betaoxidation of branched fatty acids and bile acid intermediates [6] We aimed in this study to correlate between immunohistochemical expression of AMACR in small focal prostatic carcinoma and BPH in true cut needle biopsy. 1381
3 2. MATERIALS AND METHODS: 2.1. Materials: Subjects: (carcinoma group) with age range from 40 to 102 years (mean = 68.2) and Patient (BPH group) with age range from 60 to 85 years (mean = 72.5± 3.1) obtained from the department of pathology, Ibn sena hospital and Military hospital during the period from January 2015 to January 2016 were chosen for this study Samples: A total of 50 prostate needle biopsy specimens, including 28 prostate needle biopsy specimens with prostatic adenocarcinoma and 22 BPH The diagnosis of prostate cancer was established from: Examination of multiple levels of H&Estained sections and was confirmed by two pathologists Methods: Immunohistochemical Analysis Immunohistochemical staining was carried out using streptoavidin-biotin immunoperoxidase technique (thermo fisher). Three to five micrometer thick sections, cut from formalin fixed paraffin embedded blocks, were deparaffinized in Xylene and rehydrated in graded alcohol. The mounted sections were immersed in target retrieval solution, tris buffer EDTA (PH 9.0), then boiled in this solution in PT link for 20 min and then washed in phosphate buffer saline (ph 7.2).Then the slides were then incubated 20 minute using a polyclonal anti-amacr antibody ready to use thermofisher), After a buffer rinse, bound antibodies were detected with the thermo Envision System. Slides were counterstained with hematoxylin, and rinsed again. 1382
4 The slides were allowed to air dry and were cover slipped with permanent mounting media. Negative controls, in which the primary antibodies were replaced by PBS, were carried out for each primary antibody For AMACR, prostate carcinoma was used as positive internal control. - Immunohistochemical Evaluation: - Evaluation of AMACR: Results obtained from two sections were detected by the researchers and confirmed by experienced histopathologist. Negative and positive controls were used for evaluation of the test sections. Ethical clearance for this studies is provided by ethical committee of AL-Neelain University -faculty of medical laboratory science Statistical analysis The results of the study were statistically analyzed using SPSS version15 statistical program. Data were expressed as mean± SD for quantitative variables, numbers and percentage. For categorical variables, student t test was used. For statistical analysis of Gleason's grading Spearman's statistical test was used. P< 0.05 was considered the significant limit. 3. RESULTS: Staining results with AMACR AMACR expression in malignant glands had much more extensive and intensive staining results than benign glands (P<0.001). Prostatic carcinoma showed a brown cytoplasmic granular staining pattern of AMACR in the malignant glands and cells. A total of 50 prostate needle biopsy specimens The Frequency of adenocarcinoma in all sample are28 (56%)while The Frequency of benign prosatic hyperplasia is 22(44%) 1383
5 Table (1). Four specimens of prostatic adenocarcinomas were intermediate grade gleason (5-7) and twenty four were high grade Gleason (8-10). Out of 28 cases of small biopsy of prostatic carcinoma 26 (92.8%) expressed AMACR (p504s),and 2 did not express AMACR (p504s) while in twenty two cases of small biopsy of Benign prostatic hyperplasia have only 3(13.6%) expressed AMACR (p504s) Table(2). the expression of AMACR in prostatic adenocarcinoma is more than BPH which is statically significance ((p<0.0001)) Pvalue :0.00 Table (1) showing the frequency of prostatic adenocarcinoma and begin prostatic hyperplasia among study population Sample Frequency Percent Adenocarcinoma BPH Total Table (2):-showing the frequency of expression of AMACR among study population. Diagnosis AMCAR expression Total positive Negative Adenocarcinoma BPH Total DISCUSSION: In our finding we found that the expression of AMACR in prostate cancer (92%) is more than benign prostatic hyper plasia (9.9%) but some of benign are expressed. Our finding is in agreement with study done by Yang et al., 2002; Jiang et al., 2002 Beach et al Boran et al. 2011[7]; [8]; [9]; [10] which are found, 71% of cancer cases showed positive immune-staining with AMACR, but variable 1384
6 intensities and percentages of cells were present. About 71% 100% of prostatic adenocarcinoma stained with AMACR. and also this finding is inconsistent with other study. The sensitivity of AMACR in detecting these variants was found to be 70% by Farinola and Epstein 2004 [20], 68% by Zhou et al., 2003 [21] and 77% by Zhou et al., 2003) [21] respectively. 5. CONCLUSION Using AMACR as a positive marker alone might be misleading because weak expression of AMACR might be seen in benign glands. Accordingly, one should not render a diagnosis of benignancy based solely on a negative AMACR immune-stain. 6. ACKNOWLEDGEMENT: This work was supported by the work of Asma Siddig, Zuhal Ahmed Eltayeb, Nagla Mohammed Abu Elgasim and Rania Ahmed Hassan. 7. REFERENCES: 1- Ferlay J, Bray F, Pisani P. Globocan 2002: Cancer incidence, mortality and prevalence worldwide. Version 2.0. Lyon, France: IARC Press; IARC CancerBase No Gaudin PB, Reuter VE. Benign mimics of prostatic adenocarcinoma on needle biopsy. Anat Pathol 1997;2: Hameed O, Humphrey PA. Immunohistochemistry in the diagnosis of minimal prostate cancer Current Diagnostic Pathology 2006; 12: ) 4-Thorson P, Humphrey PA. Minimal adenocarcinoma in prostate needle biopsy tissue. Am J Clin Pathol 2000;114:
7 5-Epstein JI. Diagnostic criteria of limited adenocarcinoma of the prostate on needle biopsy. Hum Pathol. 1995; 26: Ferdinandusse S, Denis S, IJlst L, Dacremont G,Waterham HR, Wanders RJ.Subcellular localization and physiological role of alphamethylacyl- CoA racemase in humans. J Lipid Res 2000; 41: Yang XJ, Wu CL, Woda BA, et al. Expression of alphamethylacyl-coa racemase (P504S) in atypical adenomatous hyperplasia of the prostate. Am J Surg Pathol 2002; 26: ) 8-Jiang Z, Wu CL, Woda BA, et al. P504S/alphamethylacyl- CoA racemase: a useful marker for diagnosis of small foci of prostatic carcinoma on needle biopsy. Am J Surg Pathol 2002; 26: ) 9-Beach R, Gown AM, De Peralta-Venturina MN, et al. P504S immunohistochemical detection in 405 prostatic specimens including gauge needle biopsies. Am J Surg Pathol 2002; 26: Boran C, Kandirali E, Yilmaz F, Serin E, Akyol M. Reliability of the 34βE12, keratin 5/6, p63, bcl-2, and AMACR in the diagnosis of prostate carcinoma. Urologic Oncology: Seminars and Original Investigations 2011; 29: Farinola MA, Epstein JI. Utility of immunohistochemistry for alpha-methylacyl- CoA racemase in distinguishing atrophic prostate cancer from benign atrophy. Hum Pathol 2004; 35: Zhou M, Jiang Z, Epstein JI. Expression and diagnostic utility of alpha-methylacyl-coaracemase (P504S) in foamy gland and pseudohyperplastic prostate cancer. Am J Surg Pathol 2003; 27:
5/21/2018. Difficulty in Underdiagnosing Prostate Cancer. Diagnosis of Prostate Cancer. Evaluation of Prostate Cancer and Atypical on Needle Biopsy
Evaluation of Prostate Cancer and Atypical on Needle Biopsy Jonathan I. Epstein Difficulty in Underdiagnosing Prostate Cancer Limited tissue on needle biopsy (1 cm. x
More informationPROSTATIC ADENOCARCINOMA: DIAGNOSTIC CRITERIA AND IMPORTANT MIMICKERS PROSTATIC ADENOCARCINOMA: DIAGNOSTIC CRITERIA
PROSTATIC ADENOCARCINOMA: DIAGNOSTIC CRITERIA AND IMPORTANT MIMICKERS PROSTATIC ADENOCARCINOMA: DIAGNOSTIC CRITERIA 1 A good H & E helps! ADENOCARCINOMA DIAGNOSTIC CRITERIA Relatively uniform proliferation
More informationHistone H1.5 Expression in Prostatic Carcinoma: An Immunohistochemical Study
Journal of Cancer Research and Treatment, 2016, Vol. 4, No. 2, 21-25 Available online at http://pubs.sciepub.com/jcrt/4/2/1 Science and Education Publishing DOI:10.12691/jcrt-4-2-1 Histone H1.5 Expression
More informationAlthough partial atrophy is one of the most common
ORIGINAL ARTICLE Partial Atrophy on Prostate Needle Biopsy Cores: A Morphologic and Immunohistochemical Study Wenle Wang, MD, PhD,* Xinlai Sun, MD,w and Jonathan I. Epstein, MD*zy Abstract: Partial atrophy
More informationThe Role of Cytokeratin 5/6 in Differential Diagnosis of Prostate Tumors
EUROPEAN ACADEMIC RESEARCH Vol. III, Issue 10/ January 2016 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) The Role of Cytokeratin 5/6 in Differential Diagnosis of Prostate
More informationP504S Immunostaining Boosts Diagnostic Resolution of Suspicious Foci in Prostatic Needle Biopsy Specimens
Anatomic Pathology / P504S/AMACR IN PROSTATIC BIOPSY SPECIMENS P504S Immunostaining Boosts Diagnostic Resolution of Suspicious Foci in Prostatic Needle Biopsy Specimens Zhong Jiang, MD, 1* Kenneth A. Iczkowski,
More informationDepartment of Diagnostic Pathology, Kochi Red Cross Hospital, Kochi, Japan
Malaysian J Pathol 2014; 36(3) : 169 173 ORIGINAL ARTICLE Application of combined immunohistochemical panel of AMACR(P504S)/p63 cocktail, cytokeratin 5 and D2-40 to atypical glands in prostatic needle
More informationPatterns of E.cadherin and Estrogen receptor Expression in Histological Sections of Sudanese Patients with Breast Carcinoma
Patterns of E.cadherin and Estrogen receptor Expression in Histological Sections of Sudanese Patients with Breast Carcinoma Hadia. Mohammed. Abdalla. Abdalrhman *, Elsadig.A.Adam, Ayda.D.A.Allatif 3,'Namareg.E.Afadul
More informationA Study to Evaluate α-methylacyl Co-A Racemase Expression in Hyperplasia and Different Grades of Adenocarcinoma of Prostate
ORIGINAL ARTICLE A Study to Evaluate α-methylacyl Co-A Racemase Expression in Hyperplasia and Different Grades of Adenocarcinoma of Prostate TAHIRA TABASSUM*, AYESHA IMTIAZ, RUKHSANA JABEEN ABSTRACT Aim:To
More informationProstate Immunohistochemistry. Literature Interpretation: Caveats. Must be aware of staining pattern of antibody in the relevant tissue
IHC Interpretation: General Principles (1) Prostate Immunohistochemistry Murali Varma Cardiff, UK wptmv@cf.ac.uk Sarajevo Nov 2013 Must be aware of staining pattern of antibody in the relevant tissue Nuclear/cytoplasmic/membranous
More informationARTHUR PURDY STOUT SOCIETY COMPANION MEETING: DIFFICULT NEW DIFFERENTIAL DIAGNOSES IN PROSTATE PATHOLOGY. Jonathan I. Epstein.
1 ARTHUR PURDY STOUT SOCIETY COMPANION MEETING: DIFFICULT NEW DIFFERENTIAL DIAGNOSES IN PROSTATE PATHOLOGY Jonathan I. Epstein Professor Pathology, Urology, Oncology The Reinhard Professor of Urological
More informationAlthough current American Cancer Society guidelines
ORIGINAL ARTICLE Diffuse Adenosis of the Peripheral Zone in Prostate Needle Biopsy and Prostatectomy Specimens Tamara L. Lotan, MD* and Jonathan I. Epstein, MD*w z Abstract: We have observed a group of
More informationDiagnostic Utility of Immunohistochemical Markers in Prostate Cancer
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-issn: 2279-0853, p-issn: 2279-0861.Volume 16, Issue 3 Ver. IX (March. 2017), PP 22-28 www.iosrjournals.org Diagnostic Utility of Immunohistochemical
More informationOptimization of the Diagnosis of Prostate Cancer by Application of Immunohistochemistry in the Gezira State, Sudan
EUROPEAN ACADEMIC RESEARCH Vol. III, Issue 10/ January 2016 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Optimization of the Diagnosis of Prostate Cancer by Application
More informationCoordinate Expression of Cytokeratins 7 and 20 in Prostate Adenocarcinoma and Bladder Urothelial Carcinoma
Anatomic Pathology / CYTOKERATINS 7 AND 20 IN PROSTATE AND BLADDER CARCINOMAS Coordinate Expression of Cytokeratins 7 and 20 in Prostate Adenocarcinoma and Bladder Urothelial Carcinoma Nader H. Bassily,
More informationCytological evaluation of effusion fluid with cell block technique and cytology smears among Sudanese patients
EUROPEAN ACADEMIC RESEARCH Vol. IV, Issue 3/ June 2016 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Cytological evaluation of effusion fluid with cell block technique
More informationROLE OF PROSTATIC BASAL CELL MARKER IN DIAGNOSIS OF PROSTATIC LESIONS
Original Research Article Pathology International Journal of Pharma and Bio Sciences ISSN 0975-6299 ROLE OF PROSTATIC BASAL CELL MARKER IN DIAGNOSIS OF PROSTATIC LESIONS SUBATHRA K* Department of pathology,
More informationCrystalloids of Prostatic Adenocarcinoma on Prostatectomy
福岡大医紀 (Med. Bull. Fukuoka Univ.):42(2),223 227,2015 Crystalloids of Prostatic Adenocarcinoma on Prostatectomy Mikio MIZOGUCHI, Hiroyuki HAYASHI, Kazuki NABESHIMA, Hiroshi IWASAKI, Morishige TAKESHITA Department
More informationPathology of the Prostate. PathoBasic Tatjana Vlajnic
Pathology of the Prostate PathoBasic 24.01.17 Tatjana Vlajnic Overview Adenocarcinoma of the prostate Grading Special variants Mimickers of prostate adenocarcinoma Atrophy Inflammatory conditions Granulomatous
More informationUSE OF IMMUNOHISTOCHEMISTRY AS AN ADJUNCT IN THE DIAGNOSIS OF LIMITED ADENOCARCINOMA OF THE PROSTATE CANCER. Jonathan Epstein
USE OF IMMUNOHISTOCHEMISTRY AS AN ADJUNCT IN THE DIAGNOSIS OF LIMITED ADENOCARCINOMA OF THE PROSTATE CANCER Jonathan Epstein The use of immunohistochemistry for basal cell markers and AMACR for the diagnosis
More information3/28/2017. Disclosure of Relevant Financial Relationships. GU Evening Subspecialty Case Conference. Differential Diagnosis:
GU Evening Subspecialty Case Conference Rajal B. Shah, M.D. VP, Medical Director, Urologic Pathology Miraca Life Sciences, Irving, Texas Clinical Associate Professor of Pathology Baylor College of Medicine,
More informationACCME/Disclosures. Cribriform Lesions of the Prostate. Case
Cribriform Lesions of the Prostate Ming Zhou, MD, PhD Departments of Pathology and Urology New York University Langone Medical Center New York, NY Ming.Zhou@NYUMC.ORG ACCME/Disclosures The USCAP requires
More informationA re-audit of Prostate biopsies from January to December 2010 and 2013.
A re-audit of Prostate biopsies from January to December 2010 and 2013. Dr. M S Siddiqui Consultant Histopathologist University Hospital of North Tees Stockton on Tees. Objectives To assess and compare
More informationThey Do Look Alike : Mimics of Prostate Cancer in Biopsy Samples
They Do Look Alike : in Biopsy Samples Gladell P. Paner, MD Departments of Pathology and Surgery (Urology) University of Chicago, IL USA Gladell.paner@uchospitals.edu Benign in Needle Biopsy 1. Benign
More informationProstatic ductal adenocarcinoma is a subtype of
ORIGINAL ARTICLE High-grade Prostatic Intraepithelial Neoplasialike Ductal Adenocarcinoma of the Prostate: A Clinicopathologic Study of 28 Cases Fabio Tavora, MD* and Jonathan I. Epstein, MD*w z Abstract:
More informationImmunohistochmical Expression of Cyclin D1 Protein in Sudanese patients with thyroid cancer
EUROPEAN ACADEMIC RESEARCH Vol. IV, Issue 2/ May 2016 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Immunohistochmical Expression of Cyclin D1 Protein in Sudanese patients
More informationGleason Scoring System 2017 JASREMAN DHILLON, MD ASSOCIATE PROFESSOR, DEPARTMENT OF ANATOMIC PATHOLOGY, MOFFITT CANCER CENTER, TAMPA, FLORIDA
Gleason Scoring System 2017 JASREMAN DHILLON, MD ASSOCIATE PROFESSOR, DEPARTMENT OF ANATOMIC PATHOLOGY, MOFFITT CANCER CENTER, TAMPA, FLORIDA Learners Objectives u Latest changes per ISUP 2014 that impact
More informationUniversity of Zagreb Medical School Repository
Središnja medicinska knjižnica Tomas, D., Krušlin, B., Rogatsch, H., Schäfer, G., Belicza, M., Mikuz, G. (00) Different Types of Atrophy in the Prostate With and Without Adenocarcinoma. European Urology,
More informationProstate cancer ~ diagnosis and impact of pathology on prognosis ESMO 2017
Prostate cancer ~ diagnosis and impact of pathology on prognosis ESMO 2017 Dr Puay Hoon Tan Division of Pathology Singapore General Hospital Prostate cancer (acinar adenocarcinoma) Invasive carcinoma composed
More informationImmunostaining was performed on tumor biopsy samples arranged in a tissue-microarray format or on
Supplemental Methods Immunohistochemical Analyses Immunostaining was performed on tumor biopsy samples arranged in a tissue-microarray format or on prostatectomy sections obtained post-study. Briefly,
More informationMuhammad Kashif Baig, Usman Hassan and Samina Mansoor
ORIGINAL ARTICLE Role of p63 in Differentiating Morphologically Ambiguous Lesions of Prostate Muhammad Kashif Baig, Usman Hassan and Samina Mansoor ABSTRACT Objective: To observe the role of p63 staining
More informationCytokeratin 5/6 expression in bladder cancer: association with clinicopathologic parameters and prognosis
https://doi.org/10.1186/s13104-018-3319-4 BMC Research Notes RESEARCH NOTE Open Access Cytokeratin 5/6 expression in bladder cancer: association with clinicopathologic parameters and prognosis Atif Ali
More informationUropathology January Jon Oxley
Uropathology January 2012 Jon Oxley Background to seminar These slides were available to view via the web from scanned slides The junior pathologists answered questions on them via the web The answers
More informationGrading Prostate Cancer: Recent Changes and Refinements
USPSTF: 2012 Report on serum PSA Screening Recommendation rating of D Reduced screening, Reduced biopsies, reduced incidence Refinements currently occurring in 2017. WHY? Grading Prostate Cancer: Recent
More information2016 WHO CLASSIFICATION OF TUMOURS OF THE PROSTATE. Peter A. Humphrey, MD, PhD Yale University School of Medicine New Haven, CT
2016 WHO CLASSIFICATION OF TUMOURS OF THE PROSTATE Peter A. Humphrey, MD, PhD Yale University School of Medicine New Haven, CT 2016 WHO CLASSIFICATION OF TUMOURS OF THE PROSTATE AUTHORS : PROSTATE CHAPTER
More informationA 53 year-old woman with a lung mass, right hilar mass and mediastinal adenopathy.
November 2015 Case of the Month A 53 year-old woman with a lung mass, right hilar mass and mediastinal adenopathy. Contributed by: Rasha Salama, M.D., IU Department of Pathology and Laboratory Medicine
More informationLIST OF ORGANS FOR HISTOPATHOLOGICAL ANALYSIS:!! Neural!!!!!!Respiratory:! Brain : Cerebrum,!!! Lungs and trachea! Olfactory, Cerebellum!!!!Other:!
LIST OF ORGANS FOR HISTOPATHOLOGICAL ANALYSIS:!! Neural!!!!!!Respiratory:! Brain : Cerebrum,!!! Lungs and trachea! Olfactory, Cerebellum!!!!Other:! Spinal cord and peripheral nerves! Eyes, Inner ear, nasal
More informationMinimal Adenocarcinoma in Prostate Needle Biopsy Tissue
Anatomic Pathology / MINIMAL PROSTATE CANCER Minimal Adenocarcinoma in Prostate Needle Biopsy Tissue Phataraporn Thorson, MD, and Peter A. Humphrey, MD, PhD Key Words: Prostate; Cancer; Biopsy; Minimal
More informationCairo University. Journal of the Egyptian National Cancer Institute.
Journal of the Egyptian National Cancer Institute (2014) 26, 15 22 Cairo University Journal of the Egyptian National Cancer Institute www.nci.cu.adu.eg www.sciencedirect.com Full Length Article Basal cell
More informationIntraductal carcinoma of the prostate on needle biopsy: histologic features and clinical significance
& 2006 USCAP, Inc All rights reserved 0893-3952/06 $30.00 www.modernpathology.org Intraductal carcinoma of the prostate on needle biopsy: histologic features and clinical significance Charles C Guo 1 and
More informationExpression of Survivin in Colorectal Tumors
EUROPEAN ACADEMIC RESEARCH Vol. III, Issue 11/ February 2016 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Expression of Survivin in Colorectal HASSN MAHJOUB MOHAMED
More informationProstate Biopsy Interpretation: An Illustrated Guide
Prostate Biopsy Interpretation: An Illustrated Guide Authors Prostate Biopsy Interpretation: An Illustrated Guide Rajal B. Shah, M.D. Director, Urologic Pathology Caris Life Sciences 6655 North MacArthur
More informationInverted (hobnail) high grade prostatic intraepithelial neoplasia and invasive inverted pattern
ONCOLOGY LETTERS 10: 2395-2399, 2015 Inverted (hobnail) high grade prostatic intraepithelial neoplasia and invasive inverted pattern MELTEM ÖZNUR 1, SEVIM BAYKAL KOCA 2, PELIN YILDIZ 3, BURAK BAHADIR 4
More informationCase: The patient is a 62 year old woman with a history of renal cell carcinoma that was removed years ago. A 2.4 cm liver mass was found on CT
Case: The patient is a 62 year old woman with a history of renal cell carcinoma that was removed years ago. A 2.4 cm liver mass was found on CT during follow- up. ALT, AST, Alk Phos and bilirubin were
More informationImmunohistochemical Expression of Angiogenic Marker CD34 in Invasive Carcinoma of the Breast
EUROPEAN ACADEMIC RESEARCH Vol. III, Issue 9/ December 2015 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Immunohistochemical Expression of Angiogenic MURTADA ABSAR
More informationAsian J Androl 2005; 7 (2): DOI: /j x
Asian J Androl 5; 7 (2): 159 163 DOI: 1.1111/j.1745-7262.5.29.x. Original Article. Mass screening of prostate cancer in a Chinese population: the relationship between pathological features of prostate
More informationDiagnosis, pathology and prognosis including variant pathology
PROSTATE CANCER Diagnosis, pathology and prognosis including variant pathology No Conflict of Interest Universitat Autónoma de Barcelona F.Algaba Section of Pathology PROSTATE CANCER Diagnosis, pathology
More informationGiant Multilocular Cystadenoma of the Prostate A Potential Pitfall On Needle Core Biopsy
ISPUB.COM The Internet Journal of Pathology Volume 8 Number 1 Giant Multilocular Cystadenoma of the Prostate A Potential Pitfall On Needle Core Biopsy R Chakravarthy, K Sikand, V Ramani, N Clarke Citation
More informationHyperplastic, Premalignant and Malignant Lesions of the Prostate Gland
Jehoram Tei Anim, md, FRCPath; Sitara Abdul Sathar, MB; Mohammed Ejaz Bhatti, MSc From the Department of Pathology, Faculty of Medicine, Kuwait University, Kuwait. Address reprint requests and correspondence
More informationIMMUNOHISTOCHEMISTRY METHOD PRECEDED BY TISSUE TRANSFER - A RELIABLE ALTERNATIVE TO CURRENT PRACTICE OF PROSTATE PATHOLOGY
IMMUNOHISTOCHEMISTRY METHOD PRECEDED BY TISSUE TRANSFER - A RELIABLE ALTERNATIVE TO CURRENT PRACTICE OF PROSTATE PATHOLOGY Denisa ANDERCO 1 *, Elena LAZAR 2, Angela BORDA 3, Sorina TABAN 2, Felix MIC 1,
More informationp53 expression in invasive pancreatic adenocarcinoma and precursor lesions
Malaysian J Pathol 2011; 33(2) : 89 94 ORIGINAL ARTICLE p53 expression in invasive pancreatic adenocarcinoma and precursor lesions NORFADZILAH MY MBBCH,* Jayalakshmi PAILOOR MPath, FRCPath,* RETNESWARI
More informationMorphologic Criteria of Invasive Colonic Adenocarcinoma on Biopsy Specimens
ISPUB.COM The Internet Journal of Pathology Volume 12 Number 1 Morphologic Criteria of Invasive Colonic Adenocarcinoma on Biopsy Specimens C Rose, H Wu Citation C Rose, H Wu.. The Internet Journal of Pathology.
More informationApplications of IHC. Determination of the primary site in metastatic tumors of unknown origin
Applications of IHC Determination of the primary site in metastatic tumors of unknown origin Classification of tumors that appear 'undifferentiated' by standard light microscopy Precise classification
More informationISPUB.COM. Interpretation Of Prostatic Biopsies: A Review. A Chitale, S Khubchandani INTRODUCTION NON-NEOPLASTIC LESIONS GRADING: GLEASON'S SCORE
ISPUB.COM The Internet Journal of Urology Volume 3 Number 1 A Chitale, S Khubchandani Citation A Chitale, S Khubchandani.. The Internet Journal of Urology. 2004 Volume 3 Number 1. Abstract The incidence
More informationExpression and Significance of GRP78 and HER-2 in Colorectal Cancer
Expression and Significance of GRP78 and HER-2 in Colorectal Cancer Qiu-xia Ji 1, Li-li Wang 2, Lin Li 3, Xiaoming Xing 2 1 Department of Pathology, Qingdao University, Qingdao, China 2 Department of Pathology,
More informationUpdate on 2015 WHO Classification of Lung Adenocarcinoma 1/3/ Mayo Foundation for Medical Education and Research. All rights reserved.
1 Our speaker for this program is Dr. Anja Roden, an associate professor of Laboratory Medicine and Pathology at Mayo Clinic as well as consultant in the Anatomic Pathology Laboratory and co-director of
More informationAppendix 1. A. Procedure for preparing histopathology slides. The liver removed and stored immediately in buffered formalin 10 % for
Appendix 1 A. Procedure for preparing histopathology slides. The liver removed and stored immediately in buffered formalin 10 % for histopathological examination. The tissue fixed for at least 48 hours
More informationASSESSMENT OF GLEASON SYSTEM USE ON DIFFERENT TYPES OF PROSTATIC TISSUE SAMPLES
ASSESSMENT OF GLEASON SYSTEM USE ON DIFFERENT TYPES OF PROSTATIC TISSUE SAMPLES I. E. PLEŞEA*, B. ZAHARIA*, S. D. ENACHE**, G. MITROI***, P. TOMESCU***, O. T. POP*, P. BADEA****, A. KOŻOKIĆ***** *Department
More informationObjective. Atypical/Atypia. Atypical Glandular Lesions of the Prostate 12/27/2011
Atypical Glandular Lesions of the Prostate V.O.Speights,Jr.,D.O. Scott and White Memorial Hospital Texas A & M Health Science Center January 13,2012 Objective To identify histological abnormalities in
More informationANATOMICAL PATHOLOGY TARIFF
ANATOMICAL PATHOLOGY TARIFF A GUIDE TO UTILISATION. The following guidelines have been agreed by consensus of Anatomical Pathologists who are members of the Anatomical Pathologist s Group, or the National
More informationSTUDY OF PROSTATIC LESION FOR A PERIOD OF FIVE YEARS
Page222 IJPBS Volume 4 Issue 2 APR-JUN 2014 222-226 Research Article Biological Sciences STUDY OF PROSTATIC LESION FOR A PERIOD OF FIVE YEARS B. Rajashekar Reddy 1, Rameswarapu Suman Babu 1 and Sujatha.P*
More informationHuman kallikrein 13 expression in salivary gland tumors
The International Journal of Biological Markers, Vol. 21 no. 2, pp. 106-110 2006 Wichtig Editore Human kallikrein 13 expression in salivary gland tumors M.R. Darling 1, L. Jackson-Boeters 1, T.D. Daley
More informationDetection of Cytomegalo Virus in Cervical Tumors among Sudanese Women
EUROPEAN ACADEMIC RESEARCH Vol. III, Issue 8/ November 2015 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Detection of Cytomegalo Virus in Cervical Tumors among Sudanese
More informationReceived: 11 Feb. 2013; Accepted: 7 Jun. 2013
ORIGINAL REPORT Inter-Observer Reproducibility before and after Web-Based Education in the Gleason Grading of the Prostate Adenocarcinoma among the Iranian Pathologists Alireza Abdollahi 1*, Sara Sheikhbahaei
More informationGross appearance of nodular hyperplasia in material obtained from suprapubic prostatectomy. Note the multinodular appearance and the admixture of
Tiền liệt tuyến Tiền liệt tuyến Gross appearance of nodular hyperplasia in material obtained from suprapubic prostatectomy. Note the multinodular appearance and the admixture of solid and microcystic areas.
More informationPSA. HMCK, p63, Racemase. HMCK, p63, Racemase
Case 1 67 year old male presented with gross hematuria H/o acute prostatitis & BPH Urethroscopy: small, polypoid growth with a broad base emanating from the left side of the verumontanum Serum PSA :7 ng/ml
More informationProstatic stromal hyperplasia with atypia (PSHA) is a
Prostatic Stromal Hyperplasia With Atypia Follow-up Study of 18 Cases Deloar Hossain, MD, FRCPC; Isabelle Meiers, MD; Junqi Qian, MD; Gregory T. MacLennan, MD; David G. Bostwick, MD, MBA Context. Prostatic
More informationACCURACY OF IMMUNOHISTOCHEMISTRY IN EVALUATION
POL J PATHOL 2011; 2: 95-100 ACCURACY OF IMMUNOHISTOCHEMISTRY IN EVALUATION OF MALIGNANT PLEURAL AND PERITONEAL EFFUSIONS FERESHTEH ENSANI, FARNAZ NEMATIZADEH, GITI IRVANLOU Department of Cytology, Cancer
More informationOutcomes Following Negative Prostate Biopsy for Patients with Persistent Disease after Radiotherapy for Prostate Cancer
Clinical Urology Post-radiotherapy Prostate Biopsy for Recurrent Disease International Braz J Urol Vol. 36 (1): 44-48, January - February, 2010 doi: 10.1590/S1677-55382010000100007 Outcomes Following Negative
More informationDetection of H. Pylori by Different Conventional Staining Methods and Immunohistochemistry in Sudanese Patients with Chronic Gastritis
Detection of H. Pylori by Different Conventional Staining Methods and Immunohistochemistry in Sudanese Patients with Chronic Gastritis Hamza.J.M.Farah 1 *, Elsadig A.Adam 2, Mohamed El-fatih 3, Mohammed.A.Abdalla
More informationInternationally indexed journal
www.ijpbs.net Internationally indexed journal Indexed in Chemical Abstract Services (USA), Index coppernicus, Ulrichs Directory of Periodicals, Google scholar, CABI,DOAJ, PSOAR, EBSCO, Open J gate, Proquest,
More informationPDF of Trial CTRI Website URL -
Clinical Trial Details (PDF Generation Date :- Sun, 25 Nov 2018 12:22:24 GMT) CTRI Number Last Modified On 06/07/2013 Post Graduate Thesis Type of Trial Type of Study Study Design Public Title of Study
More informationValue of antimesothelioma HBME 1 in the diagnosis of inflammatory and malignant pleural effusions
Romanian Journal of Morphology and Embryology 2006, 47(4):351 355 ORIGINAL PAPER Value of antimesothelioma HBME 1 in the diagnosis of inflammatory and malignant pleural effusions LILIANA MOCANU 1), ANCA
More informationPitfalls in the diagnosis of well-differentiated hepatocellular lesions
2013 Colorado Society of Pathology Pitfalls in the diagnosis of well-differentiated hepatocellular lesions Sanjay Kakar, MD University of California, San Francisco Outline Hepatocellular adenoma: new WHO
More informationOriginal Article CREPT expression correlates with esophageal squamous cell carcinoma histological grade and clinical outcome
Int J Clin Exp Pathol 2017;10(2):2030-2035 www.ijcep.com /ISSN:1936-2625/IJCEP0009456 Original Article CREPT expression correlates with esophageal squamous cell carcinoma histological grade and clinical
More informationSynonyms. Nephrogenic metaplasia Mesonephric adenoma
Nephrogenic Adenoma Synonyms Nephrogenic metaplasia Mesonephric adenoma Definition Benign epithelial lesion of urinary tract with tubular, glandular, papillary growth pattern Most frequently in the urinary
More informationExpression of the GLUT1 and p53 Protein in Atypical Mucosal Lesions Obtained from Gastric Biopsy Specimens
The Korean Journal of Pathology 2006; 40: 32-8 Expression of the GLUT1 and p53 Protein in Atypical Mucosal Lesions Obtained from Gastric Biopsy Specimens In Gu Do Youn Wha Kim Yong-Koo Park Department
More information5/21/2018. Prostate Adenocarcinoma vs. Urothelial Carcinoma. Common Differential Diagnoses in Urological Pathology. Jonathan I.
Common Differential Diagnoses in Urological Pathology Jonathan I. Epstein Prostate Adenocarcinoma vs. Urothelial Carcinoma 1 2 NKX3.1 NKX3.1 3 4 5 6 Proposed ISUP Recommendations Option to use PSA as a
More informationVENTANA MMR IHC Panel Interpretation Guide for Staining of Colorectal Tissue
VENTANA MMR IHC Panel Interpretation Guide for Staining of Colorectal Tissue VENTANA anti-mlh1 (M1) Mouse Monoclonal Primary Antibody VENTANA anti-pms2 (A16-4) Mouse Monoclonal Primary Antibody VENTANA
More informationCharacterization and significance of MUC1 and c-myc expression in elderly patients with papillary thyroid carcinoma
Characterization and significance of MUC1 and c-myc expression in elderly patients with papillary thyroid carcinoma Y.-J. Hu 1, X.-Y. Luo 2, Y. Yang 3, C.-Y. Chen 1, Z.-Y. Zhang 4 and X. Guo 1 1 Department
More informationEstrogen Receptor, Progesterone Receptor, and Her-2/neu Oncogene Expression in Breast Cancers Among Bangladeshi Women
Journal of Bangladesh College of Physicians and Surgeons Vol. 28, No. 3, September 2010 Estrogen Receptor, Progesterone Receptor, and Her-2/neu Oncogene Expression in Breast Cancers Among Bangladeshi Women
More informationDisclosures. Parathyroid Pathology. Objectives. The normal parathyroid 11/10/2012
Disclosures Parathyroid Pathology I have nothing to disclose Annemieke van Zante MD/PhD Assistant Professor of Clinical Pathology Associate Chief of Cytopathology Objectives 1. Review the pathologic features
More informationInterpretation Manual - Gastric or Gastroesophageal Junction Adenocarcinoma. PD-L1 IHC 22C3 pharmdx is FDA-approved for in vitro diagnostic use
Interpretation Manual - Gastric or Gastroesophageal Junction Adenocarcinoma PD-L1 IHC 22C3 pharmdx is FDA-approved for in vitro diagnostic use For countries outside of the United States, see the local
More informationJMSCR Vol 04 Issue 12 Page December 2016
JMSCR Vol 4 Issue 12 Page 14471-14478 December 216 www.jmscr.igmpublication.org Impact Factor 5.244 Index Copernicus Value: 83.27 ISSN (e)-2347-176x ISSN (p) 2455-45 DOI: https://dx.doi.org/1.18535/jmscr/v4i12.29
More informationSupplemental Information
Supplemental Information Prediction of Prostate Cancer Recurrence using Quantitative Phase Imaging Shamira Sridharan 1, Virgilia Macias 2, Krishnarao Tangella 3, André Kajdacsy-Balla 2 and Gabriel Popescu
More informationAssociation of Alpha Methylacyl CoA racemase Expressed withthyroid Tumors
Association of Alpha Methylacyl CoA racemase Expressed withthyroid Tumors Sanas Zaki Edrees 1, Mohammed SiddigAbdelaziz 2 1 Al Neelain university 2 Sudan University science technology, Khartoum, Sudan
More informationINTRADUCTAL LESIONS OF THE PROSTATE. Jonathan I. Epstein
INTRADUCTAL LESIONS OF THE PROSTATE Jonathan I. Epstein Topics Prostatic intraepithelial neoplasia (PIN) Intraductal adenocarcinoma (IDC-P) Intraductal urothelial carcinoma Ductal adenocarcinoma High Prostatic
More informationAtypical Foci Suspicious but not Diagnostic of Malignancy in Prostate Needle Biopsies (Also Referred to as Atypical Small Acinar Proliferation
european urology 50 (2006) 666 674 available at www.sciencedirect.com journal homepage: www.europeanurology.com Review Prostate Cancer Atypical Foci Suspicious but not Diagnostic of Malignancy in Prostate
More informationSredišnja medicinska knjižnica
Središnja medicinska knjižnica Ulamec M., Džombeta T., Čupić H., Leniček T., Tomas D., Krušlin B. (2012) Periacinar retraction clefting and d2-40 expression in prostatic adenocarcinoma. Pathology Oncology
More informationUrothelial Carcinoma (UC)
EDUCATION PD-L1 IHC 28-8 pharmdx Interpretation Manual Urothelial Carcinoma (UC) For In Vitro Diagnostic Use Table of Contents Introduction...5 Intended Use in Urothelial Carcinoma...5 How to Use the
More informationACCME/Disclosures. Diagnosing Mesothelioma in Limited Tissue Samples. Papanicolaou Society of Cytopathology Companion Meeting March 12 th, 2016
Diagnosing Mesothelioma in Limited Tissue Samples Papanicolaou Society of Cytopathology Companion Meeting March 12 th, 2016 Sanja Dacic, MD, PhD University of Pittsburgh ACCME/Disclosures GENERAL RULES
More informationAnalysis of immunohistochemical expression of CD10 in the malignant lesions of prostate
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-issn: 2279-0853, p-issn: 2279-0861.Volume 16, Issue 7 Ver. I (July. 2017), PP 78-82 www.iosrjournals.org Analysis of immunohistochemical expression
More informationContemporary Update on Pathology-related Issues on Routine Workup of Prostate Biopsy
AND QUANTITATIVE CYTOPATHOLOGY AND AQCHANALYTICAL HISTOPATHOLOGY An Official Periodical of The International Academy of Cytology and the Italian Group of Uropathology ARTICLES Contemporary Update on Pathology-related
More informationModern Pathology (2011) 24, & 2011 USCAP, Inc. All rights reserved /11 $
& 2011 USCAP, Inc. All rights reserved 0893-3952/11 $32.00 185 Luminal cytokeratin expression profiles of breast papillomas and papillary carcinomas and the utility of a cytokeratin 5/p63/cytokeratin 8/18
More informationMucin-producing urothelial-type adenocarcinoma of prostate: report of two cases of a rare and diagnostically challenging entity
& 2005 USCAP, Inc All rights reserved 0893-3952/05 $30.00 www.modernpathology.org Mucin-producing urothelial-type adenocarcinoma of prostate: report of two cases of a rare and diagnostically challenging
More informationWT1, Estrogen Receptor, and Progesterone Receptor as Markers for Breast or Ovarian Primary Sites in Metastatic Adenocarcinoma to Body Fluids
Anatomic Pathology / WT1, ESTROGEN RECEPTOR, AND PROGESTERONE RECEPTOR IN CYTOLOGY OF BODY FLUIDS WT1, Estrogen Receptor, and Progesterone Receptor as Markers for Breast or Ovarian Primary Sites in Metastatic
More informationComparison of immunohistochemical markers between adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma
509 Journal of Oral Science, Vol. 51, No. 4, 509-514, 2009 Original Comparison of immunohistochemical markers between adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma Nasrollah Saghravanian
More informationCell Culture. The human thyroid follicular carcinoma cell lines FTC-238, FTC-236 and FTC-
Supplemental material and methods Reagents. Hydralazine was purchased from Sigma-Aldrich. Cell Culture. The human thyroid follicular carcinoma cell lines FTC-238, FTC-236 and FTC- 133, human thyroid medullary
More informationCase history: Figure 1. H&E, 5x. Figure 2. H&E, 20x.
1 Case history: A 49 year-old female presented with a 5 year history of chronic anal fissure. The patient s past medical history is otherwise unremarkable. On digital rectal examination there was a very
More informationSignet-Ring Cell Change in Benign Prostatic Hyperplasia - A Rare Case Report
Article ID: WMC00688 ISSN 2046-1690 Signet-Ring Cell Change in Benign Prostatic Hyperplasia - A Rare Case Report Author(s):Dr. Pallavi Bhuyan, Dr. Smita Mahapatra, Dr. Sujata Pujari, Dr. Jayasree Rath
More information