Bioactive glucosinolates and antioxidant properties of broccoli seeds cultivated in Thailand
|
|
- Edgar Watson
- 5 years ago
- Views:
Transcription
1 ORIGINAL ARTICLE of broccoli seeds cultivated in Thailand Sarunya Chuanphongpanich 1, Sukon Phanichphant 2, Duang Bhuddasukh 3, Maitree Suttajit 4 and Busaban Sirithunyalug 5 Abstract Chuanphongpanich, S., Phanichphant, S., Bhuddasukh, D., Suttajit, M. and Sirithunyalug, B. of broccoli seeds cultivated in Thailand, 2006, 28(Suppl. 1) : One of the most significant health concerns of cruciferous vegetables is the presence of biologically active compounds, glucosinolates. Broccoli (Brassica oleracea var. italica) is a nutritionally important crop grown all over the world. Glucosinolates have been found to have anti-cancer properties. The primary purpose of this study was to evaluate glucosinolate content and antioxidant property in the seeds of broccoli cultivars widely grown in Thailand. Glucosinolates were analyzed with high performance liquid chromatography (HPLC). Total and individual glucosinolate levels varied significantly among cultivars. In all broccoli seeds, 4-methylsulfinylbutylglucosinolate (glucoraphanin) was the predominant glucosinolate. The highest total glucosinolates was 65.5 µmol/g DW in Top Green #067 cultivar, followed by Packman (58.6), Green Queen (51.2), Pak Ging (25.5) and Rod Fai (20.3). The antioxidant capacities, including ABTS radical scavenging activity and ferrous ion chelating ability in the methanol and water extracts, were found to be high. Key words : ABTS assay, broccoli seeds, cultivar, FRAP assay, glucosinolates 1 M. Pharm. (Pharmaceutical Chemistry), Ph.D. Candidate in Pharmacy, 4 Dr. rer. nat. (Pharmaceutical Chemistry), Assoc. Prof., Department of Pharmaceutical Science, Faculty of Pharmacy, 2 Ph.D.(Chemistry), Assoc. Prof., Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 3 Ph.D.(Biochemistry), Prof., Department of Chemistry, Faculty of Science, Mahasarakham University, Kantharawichai, Mahasarakham, Thailand. Corresponding sarunyag @yahoo.com Received, 9 December 2004 Accepted, 26 December 2005
2 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 56 The presence of xenobiotics and/or the absence of some essential nutrients in our diet can produce or enhance a certain susceptibility to illness. Nutraceutical and functional food ingredients have been popularly used in health products. The most important nutraceutical formulations contain vitamins and minerals in combination with antioxidant(s) such as carotenoids, omega-3 fatty acids, glucosinolates and/or degradants in the finished products (Palmieri, 2000). Glucosinolates, a class of plant secondary metabolite, are β-thioglucoside N-hydroxysulfates [also known as (Z)-(or cis)-nhydroximinosulfate esters or S-glucopyranosyl thiohydroximates], with a side chain (R) and a sulfur-linked β-d-glucopyranose moiety. The common structural feature of intact glucosinolates is shown in Figure 1 (Fahey et al., 2001). Glucosinolates and/or their breakdown products have long been known for their fungicidal, bacteriocidal, nematocidal, allelopathic properties (intercrops or forecrops for weed control) and have attracted intense research interest because of their cancer chemoprotective attributes (Fahey et al., 2001; Verhoeven et al., 1997). Glucosinolates are found in dicot plants, particularly in the Order Capparales, comprising the Capparaceae, Brassicaceae (Cruciferae), Loeberliniaceae, Moringaceae, Rasedaceae and Tovariaceae. Brassicaceae (Cruciferae) is one of the 10 most economically important plant families and includes vegetables such as broccoli, brussels sprouts, cabbage, cauliflower, kale, radish and various mustards. Glucosinolate content in some tissues of the Brassica vegetables is approximately 1% of dry weight (DW), although the content has been found to be as high as 10% in the seeds of some plants. Among the cultivated Brassicaceae, broccoli attracted attention after the discovery that it contains high levels of isothiocyanate sulforaphane [4-(methylsulfinyl) butyl isothiocyanate], produced from the breakdown of the glucosinolate glucoraphanin [4-(methylsulfinyl) butyl glucosinolate] and showed great potential anticarcinogenic properties. Sulforaphane is the most potent naturally occurring Phase II enzyme inducer and considered to play an important role in the detoxification of xenobiotic compounds when assimilated with diet (Fahey et al., 2001; Rosa et al., 1997). Broccoli grows well in most types of soil, but rather prefers those crumbling or friable soils with ph around , high soil humidity, and sunlight. It develops best during cool seasons. The optimal temperatures are between 18-27ºC, but preferably at the average temperature of 20ºC (George, 1999). In Thailand, the most suitable period is between November and December. There are many types of F1 hybrid broccoli cultivars in Thailand. F1 hybrid cultivars are the results of crossing two inbred lines, which have been maintained under strict control, or under the supervision of commercial plant breeders, which are known to produce a desirable hybrid. The advantages of F1 hybrid broccoli cultivars include uniformity, increased vigor, earliness, higher yield and resistance to specific pests and pathogens. This research focused specifically on the determination of glucosinolate content and antioxidant properties in the seeds of various Thai broccoli cultivars. Materials and Methods Seed materials Seeds from five broccoli cultivars, which included Green Queen, Packman, Pak Ging, Figure 1. General structure of intact glucosinolates
3 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 57 Rod Fai, and Top Green #067 obtained from Phu Ruea Highland Cultivation Experimental Station, Loei, Thailand, were used in the study. The seeds were harvested in May 2003, and were sealed in plastic-laminated aluminum sachets. The sachets were stored at 4ºC until use. Broccoli seeds were sown directly in seedbeds or containers. Young plants were planted out into the field when they had reached the 5-7 true leaf stage. Under favorable environmental conditions, it took d for broccoli, depending on cultivars, to grow from seedling to harvest. Chemicals and equipment ABTS (2,2 -azinobis-3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was obtained from Sigma-Aldrich Chemical Co., St. Louis. Ascorbic acid was purchased from Fisher, UK, and other chemicals were potassium persulfate (Carlo Erba Reagenti, Italy), Trolox (Fluka, Switzerland), quercetin dihydrate (Arcos organics, Belgium), and iron (III) chloride heptahydrate (BDH, Englnd). Sulfatase, DEAE Sephadex A25, benzylglucosinolate and TPTZ (2,4,6-Tris(2- pyridyl)-s-triazine) were purchased from Sigma Chemical Co., St. Louis. Certified rapeseed reference materials BCR-367R was obtained from Commission of the European Community Bureau of References (Brussels, Belgium). All other chemicals and solvents were obtained from Sigma Chemical Co., St. Louis, or J.T Baker, Phillipsburg, NJ. The HPLC system was equipped with a Hewlett Packard Model HP 1090 Photodiode Array Detector, and an automated injector. Data were processed with HP ChemStation software. Glucosinolate assay The AOCS methods (AOCS, 1993) were modified for glucosinolate analysis. Broccoli seeds were homogenized with a mortar and pestle. A 0.2 g of ground sample from each replicate was placed in a capped 15 ml glass tube and heated on a heating block at 75ºC for 1 min. Warm methanol (2 ml) was then added to the test tube followed by 0.25 ml of benzylglucosinolate (1 mg/ml) as an internal standard. The tube was continuously heated for 10 min and centrifuged at 3000g for 3 min. After centrifugation, the supernatant was transferred to a 10 ml flask. The residue was extracted twice with 2 ml of warm methanol and the solution was centrifuged each time. The supernatant was collected, combined with the previously saved supernatant, and mixed thoroughly in the same flask. One ml of the supernatant was transferred to DEAE Sephadex A-25 column and allowed to drain and desulfoglucosinolates were obtained after the treatment of the column with sulfatase (500 µl). The column was capped for 12 h. Desulfated glucosinolates were eluted off the column with three 1 ml portion of water and separated by HPLC on an analytical 5 µlichrosorb Hibar RP-18 column (Merck, Darmstat, Germany) with a mobile phase of acetonitrile (50%) plus deionized water (50%) containing 5 mm tetraoctylammonium bromide (solvent A) and acetonitrile (solvent B). The flow rate was 0.20 ml/min at 229 nm wavelength. The following gradient elution was used for separation: 0%B at 0 min, 100%B at 23 min. Solvents were filtered (0.45 µm) and degassed for 30 min before use. Individual glucosinolates were identified by comparison of retention times with those of known reference compounds and by adding separate, individual, pure compounds to broccoli seeds extracts and observing the rise in peak height. For data analysis, analyses of variance (ANOVA) were performed using a general linear model test for each cultivar. Mean separation was determined by least significant differences (Fisher's LSD) at P=0.05. Antioxidant assay Preparation of sample extracts Broccoli seeds were milled and, in turn, extracted with five solvents: chloroform, ethyl acetate, ethanol (70%), methanol and water. Twostep extraction was applied by shaking flasks with 10±0.01 g of seed and 100 ml (50+50) of solvent in a shaking machine (Shaking Water Bath, model SB , Thailand). Each extraction step was completed in 3 h. The extracts were filtered, concentrated in a rotary evaporator (Buchi, Flawil,
4 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 58 Switzerland) at approximately 40ºC and tested for antioxidant activities. I) ABTS radical cation decolourisation assay ABTS radical cation decolourisation test (Re et al., 1999) is also a spectrophotometric method widely used for the assessment of antioxidant activity of various substances. ABTS radical was generated by oxidation of ABTS with potassium persulfate. Three ml of ABTSº + cation solution were mixed with 30 ml seed extract solution in 1 cm path length disposable microcuvette. The absorbance at 734 nm was determined after reaction for 4 min. The lower the absorbance the stronger the reducing power compared with three known antioxidants. The antioxidants included ascorbic acid as a vitamin widely found in fruits and vegetables, quercertin representing the group of polyphenols, and Trolox as water-soluble vitamin E analogue. The percentage of ABTS scavenging activity is expressed by [1-(test sample absorbance/ blank sample absorbance)] x 100. II) Ferric reducing antioxidant power (FRAP assay) The FRAP assay as described by Benzie and Strain (1996) was used with minor modifications. A 50 ml seeds extraction solution and 30 ml water were pipetted into a 1 cm path length disposable microcuvette. A 200 ml FRAP solution (300 mm acetate buffer ph 3.6: 10 mm TPTZ in 40 mm HCl: 20 mm FeCl 3 ; 10:1:1) was added, mixed for 10 s and the absorbance at 593 nm was taken after 4 min. The higher the ferrous ion chelating ability of the test sample the lower the absorbance. The percentage of ferrous ion chelating ability is expressed by [1-(test sample absorbance/ blank sample absorbance)] x 100. Results and Discussion Content of bioactive glucosinolates in broccoli seeds At least seven glucosinolates were detected in the seeds, which were classified into three categories: (1) methylsulfinylalkyl glucosinolates (glucoraphanin, glucoiberin, glucoalyssin); (2) methylthioalkyl glucosinolates (glucoerucin, glucoberverin, glucoalyssin); and (3) indole glucosinolates (4-hydroxyglucobrassicin, neoglucobrassicin). Total and individual glucosinolates levels varied significantly among cultivars. The compound 4-methylsulfinylbutyl glucosinolate (glucoraphanin) was the predominant glucosinolate in broccoli seeds. Total glucosinolates were significantly higher in 'Top Green #067' (64.5 µmol/g DW). Those of other cultivars are, in descending order, as follow: 'Green Queen' (51.2 µmol/g DW), 'Packman' (44.3 µmol/g DW), 'Pak Ging' (25.5 µmol/g DW) and 'Rod Fai' (20.3 µmol/g DW). All five cultivars were planted in the same environment and there was no special treatment to the soil bases. A typical glucosinolate chromatogram from broccoli seeds is shown in Figure 2. The glucosinolate pattern of all cultivars was similar to that described by other authors (Kushad et al., 1999; Rosa et al., 1997; Rosa and Rodrigues, 2001). Table 1 shows the amount of the total and main individual glucosinolate expressed in term of µmol/g DW of the seeds. 'Top Green #067' contained the highest level of total and individual glucosinolates, with glucoraphanin at 48.9 µmol/g DW accounting for 75.8% of the glucosinolates. The compounds 4-methylsulfinylbutyl-, 4-methoxyindol-3-ylmethyl, 4-methylthiobutyl and 4-hydroxy-3-indolylmethyl glucosinolate were common to all cultivars. Only 1-methoxyindol-3-ylmethyl could not be detected in 'Rod Fai' cultivar. Glucoraphanin concentrations in broccoli seeds ranged from 11.4 to 48.9 µmol/g DW, while neoglucobrassicin ranged from 0.0 to 0.2 µmol/g DW. Glucoerucin concentration was lowest in 'Pak Ging' (5.2 µmol/g DW) and highest in 'Top Green' (11.0 µmol/g DW), which was slightly higher than 'Packman' (10.2 µmol/g DW). The concentration of 4-methoxyglucobrassicin was highest in 'Top Green #067' (1.8 µmol/g DW) and lowest in 'Pak Ging' and 'Green Queen' (1.3 µmol/ g DW). ABTS radical scavenging activity of the various broccoli seed extracts Antioxidant activity of broccoli seed extracts
5 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 59 Figure 2. A typical glucosinolate chromatogram from broccoli seeds. Peaks 1: 3-methylsulfinylpropyl-; 2: 4-methylsulfinylbuty-; 3: 5-methylsulfinylpropyl-; 4: 4-hydroxy- 3-indolylmethyl-; 5: 4-methylthiobutyl-; 6: 4-methoxy-3-indolymethyl-; 7: 1- methoxy-3-indolylmethyl glucosinolate. Tabel 1. Total and main individual glucosinolate levels (µmol/g DW) of the five cultivars of broccoli seeds. Glucosinolates Cultivars Green King Packman Pak Ging Rod Fai TopGreen #067 Total glucosinolates methylsulphinylbutyl hydroxy-3-indolylmethyl methylthiobutyl (glucoerucin) methoxy-3-indolymethyl (4-Methoxyglucobrassicin) 1-methoxy-3-indolylmethyl (neoglucobrassicin) equivalent to that of the three standard antioxidants, i.e. ascorbic acid, quercertin and Trolox, using the ABTS assay, is shown in Figure 3. Results showed that in general the methanolic extract of broccoli seeds gave the highest ABTS radical scavenging activity followed by that of water, ethanol (70%), ethyl acetate and chloroform extracts. The chloroform extracts showed the least scavenging activity (<25%). This activity increased by increasing the concentration of the extracts. The TEAC value of ascorbic acid is highest in 'Pak Ging' (33.2%) followed by 'Packman' (30%), 'Top Green #067' (29.8%), 'Green Queen' (27.2%) and 'Rod Fai' (23%). The TEAC value of quercetin is highest in 'Green Queen' (71.2%) followed by 'Top Green #067' (67.1%), 'Packman' (64.5%), 'Pak Ging' (60.3%) and 'Rod Fai' (53.2%). The TEAC value of Trolox is highest in 'Packman' (39.2%) followed by 'Pak Ging' (38.3%), 'Top Green #067' (36.4%), 'Green Queen' (25.9%) and 'Rod Fai' (19.2%). The highest overall antioxidant activity was in 'Top Green #067' cultivar. Ferrous ion chelating ability of the broccoli seed extracts Figure 4 demonstrates the ferrous ion chelating ability of the broccoli seeds extracted with five different solvents. The methanolic seed extract performed the best ferrous ion chelating ability followed by water, ethanol (70%), ethyl acetate and
6 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 60 Figure 3. Equivalent antioxidant activity of broccoli seeds extracted with five different solvents as compared to the three standard antioxidants, i.e. ascorbic acid (a), quercetin (b) and Trolox (c), using ABTS assay Figure 4. Ferrous ion chelating power of broccoli seeds extracted with five different solvents.
7 Vol.28 (Suppl.1), 2006: Nutraceutical and Functional Food 61 chloroform extracts. 'Packman' showed the highest ferrous ion chelating ability (46.67%), followed by 'Top Green#067' (44.26%), 'Green Queen' (39.21%), 'Pak Ging' (37.65%) and 'Rod Fai' (16.46%). The results presented in Figures 3 and 4 showed that extraction with methanol and water could give the broccoli seed extracts a higher ABTS radical scavenging activity and ferrous ion chelating ability, though the reason is unclear and needs to be further studied. Ethyl acetate and chloroform extracts were considerably less effective as radical scavenger compared to methanolic extracts. Ethyl acetate extracts were more effective against the ABTS radical than chloroform extracts. As the polarity of these two solvents is quite similar (ethyl acetate is slightly more polar than chloroform) it can be concluded that chloroform is not a suitable solvent for the extraction of radical scavenging compounds from broccoli seeds. Methanol may be the best solvent for such purpose. This result is in accordance with the findings of other researchers (Guo et al., 2001; Schlesier et al., 2002). Conclusions This study has shown that glucosinolates concentrations varied among four Thai broccoli seed cultivars. The highest glucoraphanin concentration was found in 'Top Green #067' cultivar. The antioxidant properties of the four broccoli cultivars, determined by ABTS radical scavenging activity and ferrous ion chelating ability, also showed 'Top Green #067' to be the best. Methanol and water were shown to be the most suitable solvents for the extraction of the antioxidants from broccoli seeds. Acknowledgments This study was supported by the National Research Council of Thailand and the Research Fund from the Graduate School, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. References AOCS Official Method Ak 1-92, Determination of glucosinolates content in rapeseed and canola by HPLC. Benzie, I.F.F. and Strain, J.J The Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal. Biochem., 229: Fahey, J.W., Zalcmann, A.T. and Talalay, P The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochem., 56: George, R.A.T Vegetable Seed Production. 2 nd ed., Oxon, CABI Publishing, New York. Guo, J.T., Lee, H.L., Chiang, S.H., Lin, F.I. and Chang, C.Y Antioxidant properties of the extracts from different parts of broccoli in Taiwan. J. Food Drug Anal., 9(2): Kushad, M.M., Brown, A.F., Kurilich, A.C., Juvik, J.A., Klein, B.P., Wallig, M.A. and Jeffery, E.H Variation of glucosinolates in vegetable crops of Brassica oleracea. J. Agr. Food Chem., 47: Palmieri, S Glucosinolates nutraceutical product? Agro-Food Ind. Hi-Tech., 7: 1-6. Re, R., Pellegrini, N., Proteggemnte, A., Pannala, A., Yang, M. and Rice-Evans, C Antioxidant activity applying and improved ABTS radical cation decolorization assay. Free Radical Biol. Med., 26(9/10): Rosa, E.A.S., Hesney, R.K., Fenwick, G.R. and Portas, C Glucosinolates in crop plants. Hort. Rev., 19: Rosa, E.A.S. and Rodrigues, A.S Total and individual glucosinolate content in 11 broccoli cultivars grown in early and late seasons. Hort. Sci., 36(1): Schlesier, K., Harwat, M., Bohm, V. and Bitsch, R Assessment of antioxidant activity by using different in vitro methods. Food Radical Res., 36(2): Verhoeven, D.T., Verhagen, H., Goldbohm, R.A., van den Brandt, P.A. and van Poppel, G A review of mechanisms underlying anticarcinogenicity by brassica vegetables. Chem. Biol. Int., 103:
Chiang Mai J. Sci. 2006; 33(1) : Contributed Paper
Chiang Mai J. Sci. 2006; 33(1) : 117-122 www.science.cmu.ac.th/journal-science/josci.html Contributed Paper Antioxidant Capacity of Broccoli Seeds Grown in Thailand Sarunya Chuanphongpanich* [a], Maitree
More informationKey Words: Brassica oleraceae, glucosinolate, liquid chromatography mass spectrometry, FNH-I-003
IDENTIFICATION OF MAJOR GLUCOSINOLATES IN BROCCOLI (Brassica oleracea var. italica) BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY (LC-MS) AND DETERMINATION OF ANTICANCER PROPERTIES OF BROCCOLI EXTRACTS Carlos
More informationAntioxidative activities and phenolic compounds of pumpkin (Cucurbita pepo) seeds and amaranth (Amaranthus caudatus) grain extracts
SUPPLEMENTARY MATERIAL Antioxidative activities and phenolic compounds of pumpkin (Cucurbita pepo) seeds and amaranth (Amaranthus caudatus) grain extracts Pier Giorgio Peiretti a, Giorgia Meineri b, Francesco
More informationAn Investigative Study of Reactions Involving Glucosinolates and Isothiocyanates
An Investigative Study of Reactions Involving Glucosinolates and Isothiocyanates Alzea Chrisel H. Alea 1, Diane Elaine T. Co 2 and Marissa G Noel 3* 1,2,3Chemistry Department, De La Salle University, 2401
More informationR. Amarowicz ANTIOXIDANT PROPERTIES OF RAPESEED CAN BE MODIFIED BY CULTIVATION AND BIOLOGICAL STRESS
ANTIOXIDANT PROPERTIES OF RAPESEED CAN BE MODIFIED BY CULTIVATION AND BIOLOGICAL STRESS R. Amarowicz Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
More informationSTANDARD OPERATING PROTOCOL (SOP)
1 STANDARD PERATING PRTCL (SP) Subject: Determination of Flavonol Glycosides in Ginkgo biloba Products by HPLC Analysis Project/Core No.: Core B Total 6 Pages SP No.: CB0104 Modified Date: 07/30/01 BTANICAL
More informationRelative Measurement of Zeaxanthin Stereoisomers by Chiral HPLC
Relative Measurement of Zeaxanthin Stereoisomers by Chiral HPLC Principle To measure the relative percentages of the (3R,3 R), (3R,3 S) and (3S,3 S) stereoisomers of zeaxanthin in dietary ingredient and
More informationPAPRIKA EXTRACT SYNONYMS DEFINITION DESCRIPTION FUNCTIONAL USES CHARACTERISTICS
PAPRIKA EXTRACT Prepared at the 77 th JECFA, published in FAO JECFA Monographs 14 (2013), superseding tentative specifications prepared at the 69 th JECFA (2008). An ADI of 0-1.5 mg/kg bw was allocated
More informationSTANDARD OPERATING PROTOCOL (SOP)
1 STANDARD PERATING PRTCL (SP) Subject: To determine the Contents of isoflavone in soybean products by HPLC Analysis Project/Core No.: Core B Total 9 Pages SP No.: CB0102 Modified Date: 05/15/01 BTANICAL
More informationFlupyradifurone. HPLC Method
HPLC Method CIPAC Collaboration Trial according to CIPAC Information Sheet No 308 by Alexandra Michel Crop Science Division Bayer Aktiengesellschaft Alfred-Nobel-Str. 50, Building 6820 40789 Monheim am
More informationSUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix
SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix Dorota Woźniak A, Andrzej Dryś B, and Adam Matkowski* A A Department of Pharmaceutical Biology
More informationASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC
ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry
More informationJagua (Genipin-Glycine) Blue (Tentative)
0 out of 9 Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 84th meeting 2017 Jagua (Genipin-Glycine) Blue (Tentative) This monograph was also
More informationISSN: ; CODEN ECJHAO E-Journal of Chemistry 2011, 8(3),
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1275-1279 Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine
More informationCHARACTERIZATION OF THE GLUCOSINOLATES AND ISOTHIOCYANATES IN MALUNGGAY
CHARACTERIZATION OF THE GLUCOSINOLATES AND ISOTHIOCYANATES IN MALUNGGAY (Moringa oleifera L.) EXTRACTS AND DETERMINATION OF THEIR MYROSINASE ACTIVITY AND ANTICANCER PROPERTIES Raymond S. Malabed 1 and
More informationLutein Esters from Tagetes Erecta
Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Lutein Esters from Tagetes Erecta This monograph was also published in: Compendium
More informationCHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS
CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
More informationPreparation and characterization of Aloe vera extract
Chapter 2 Preparation and characterization of Aloe vera extract 2.1. INTRODUCTION Aloe vera ethanolic extract was prepared according to Noor et.al, 2008 with few modifications. The phytochemical analysis
More informationARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) ARTESUNATI COMPRESSI ARTESUNATE TABLETS
December 2009 ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical
More informationHPLC Analysis of Sugars
HPLC Analysis of Sugars Pre-Lab Exercise: 1) Read about HPLC, sugars and the experiment and its background. 2) Prepare a flowchart as appropriate for the lab exercise. 3) Note the various sugar concentrations
More informationAnalysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection
APPLICATION NOTE Liquid Chromatography Author: Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Cannabis by UHPLC Using PDA Detection Introduction Cannabis sativa, from which
More informationIsolation of five carotenoid compounds from tangerine tomatoes
Isolation of five carotenoid compounds from tangerine tomatoes Thesis Thomas Haufe Advisor: Steven J. Schwartz, Ph.D Department of Food Science and Technology Presented in fulfillment of the requirements
More informationTitle Revision n date
A. THIN LAYER CHROMATOGRAPHIC TECHNIQUE (TLC) 1. SCOPE The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate and triamcinolone acetonide
More informationAnalytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products)
Analytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products) The target compound to be determined is 2, 4, 5-T. 1. Instrument Liquid Chromatograph-tandem mass spectrometer (LC-MS/MS)
More informationOptimization of extraction method and profiling of plant phenolic compounds through RP-HPLC
Chapter III Optimization of extraction method and profiling of plant phenolic compounds through RP-HPLC 1. INTRODUCTION Phenolics compounds are naturally present antioxidants, found in a variety of plant
More informationGlucosinolates in Brassica foods: bioavailability in food and significance for human health
Phytochem Rev (2008) 7:213 229 DOI 10.1007/s11101-007-9072-2 Glucosinolates in Brassica foods: bioavailability in food and significance for human health María Elena Cartea Æ Pablo Velasco Received: 11
More informationRP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical
More informationAnalysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinElmer Altus UPLC System with PDA Detection
APPLICATION NOTE Liquid Chromatography Author: Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Phenolic Antioxidants in Edible Oil/Shortening Using the PerkinElmer Altus UPLC System with PDA
More informationVariation in content of bioactive components in broccoli
Journal of Food Composition and Analysis 16 (2003) 323 330 JOURNAL OF FOOD COMPOSITION AND ANALYSIS www.elsevier.com/locate/jfca Study review Variation in content of bioactive components in broccoli E.H.
More informationAnalysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector
application Note UHPLC Author Njies Pedjie PerkinElmer, Inc. Shelton, CT 06484 USA Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector Introduction Foods
More informationKeywords: antioxidant; extraction; paper flower; phenolic compound
PHENOLIC ANTIOXIDANTS FROM Bougainvillea SPP. Punyawatt Pintathong 1, *, Prisana Pinket 1, Monthira Papoodplook 1, Natthawut Thitipramote 1,2, Phanuphong Chaiwut 1 1 School of Cosmetic Science, Mae Fah
More informationOptimization of extraction the red cabbage extract with ultrasound technology, assisted by response surface method
International Journal of Biosciences IJB ISSN: 2220-6655 (Print), 2222-5234 (Online) http://www.innspub.net Vol. 6, No. 3, p. 94-100, 2015 RESEARCH PAPER OPEN ACCESS Optimization of extraction the red
More informationGlucosinolate Content and Myrosinase Activity in Rapid-cycling Brassica oleracea Grown in a Controlled Environment
J. AMER. SOC. HORT. SCI. 129(3):321 330. 2004. Glucosinolate Content and Myrosinase Activity in Rapid-cycling Brassica oleracea Grown in a Controlled Environment Craig S. Charron and Carl E. Sams 1 Department
More informationRP- HPLC and Visible Spectrophotometric methods for the Estimation of Meropenem in Pure and Pharmaceutical Formulations
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.2, pp 605-609, April-June 2011 RP- HPLC and Visible Spectrophotometric methods for the Estimation of Meropenem
More informationDetermination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography
Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Application ote Food Safety Authors Chen-Hao Zhai and Yun Zou Agilent Technologies Co. Ltd.
More informationMETHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Ramalakshmi et al. SJIF Impact Factor 6.647 Volume 7, Issue 2, 1010-1018 Research Article ISSN 2278 4357 METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC
More informationA -GLS Arabidopsis Cuscuta gronovii
-GLS Arabidopsis Cuscuta gronovii internal standard 4MS Wildtype Arabidopsis Cuscuta gronovii base Cuscuta gronovii apex 3MSP internal standard MSP 4MT 8MSO 4MO 1MO Figure S1. Representative HPLC-DAD chromatograms
More informationApplication Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract.
Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling Application Note Food 1 Agilent 12 Infinity 1 Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in
More informationOPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM LEAVES AND STEMS OF THAI WATER SPINACH (Ipomoea aquatic var.
OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM LEAVES AND STEMS OF THAI WATER SPINACH (Ipomoea aquatic var. aquatica) Boonnet Koonchanok 1, Thadsaneeya Cheunchob 1, Nisakorn
More informationStability indicating RP-HPLC method development and validation of Etizolam and Propranolol hydrochloride in pharmaceutical dosage form
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationApplication Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.
Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2
More informationDevelopment and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):236-242 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationTenofovir disoproxil fumarate (Tenofoviri disoproxili fumaras)
C 19 H 30 N 5 O 10 P. C 4 H 4 O 4 Relative molecular mass. 635.5. Chemical names. bis(1-methylethyl) 5-{[(1R)-2-(6-amino-9H-purin-9-yl)-1-methylethoxy]methyl}-5-oxo-2,4,6,8-tetraoxa-5-λ 5 - phosphanonanedioate
More informationThis revision also necessitates a change in the table numbering in the test for Organic Impurities.
Methylphenidate Hydrochloride Extended-Release Tablets Type of Posting Notice of Intent to Revise Posting Date 27 Jul 2018 Targeted Official Date To Be Determined, Revision Bulletin Expert Committee Chemical
More informationRebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica
Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica This
More informationRITONAVIRI COMPRESSI RITONAVIR TABLETS. Final text for addition to The International Pharmacopoeia (July 2012)
July 2012 RITONAVIRI COMPRESSI RITONAVIR TABLETS Final text for addition to The International Pharmacopoeia (July 2012) This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications
More informationApplication Note Soy for Isoflavones by HPLC. Botanical Name: Glycine max L. Common Names: Parts of Plant Used: Beans.
Application Note 0066 - Soy for Isoflavones by HPLC As published in The Handbook of Analytical Methods for Dietary Supplements Botanical Name: Glycine max L. Common Names: Soybean Parts of Plant Used:
More informationTENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010)
June 2010 TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical
More informationDetermination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.
Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are
More informationThe Nitrofurantoin Capsules Revision Bulletin supersedes the currently official monograph.
Nitrofurantoin Capsules Type of Posting Revision Bulletin Posting Date 28 Dec 2018 Official Date 01 Jan 2019 Expert Committee Chemical Medicines Monographs 1 Reason for Revision Compliance In accordance
More information4. Determination of fat content (AOAC, 2000) Reagents
94 ANALYTICAL METHODS 1. Determination of moisture content (AOAC, 2000) 1. Dry the empty dish and lid in the oven at 105 C for 3 h and transfer to desiccator to cool. Weigh the empty dish and lid. 2. Weigh
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationTramadol Hydrochloride Extended-Release Tablets. Expert Committee Chemical Medicines Monographs 2 Reason for Revision Compliance
Tramadol Hydrochloride Extended-Release Tablets Type of Posting Revision Bulletin Posting Date 27 May 2016 Official Date 01 Jun 2016 Expert Committee Chemical Medicines Monographs 2 Reason for Revision
More informationCarotenoid Extraction and Quantification from Capsicum annuum Richard D. Richins, James Kilcrease, Laura Rodgriguez-Uribe and Mary A.
Carotenoid Extraction and Quantification from Capsicum annuum Richard D. Richins, James Kilcrease, Laura Rodgriguez-Uribe and Mary A. O Connell * Plant and Environmental Sciences, New Mexico State University,
More informationA simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form
IJPAR Vol.4 Issue 1 Jan-Mar-2015 Journal Home page: ISSN: 2320-2831 Research article Open Access A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical
More informationApplication Note. Treatment of poor memory, memory loss, Alzheimer s disease, peripheral vascular disease.
Application Note As published in The Handbook of Analytical Methods for Dietary Supplements 0047 - Ginkgo for Flavonoids and Terpenes by HPLC Botanical Name: Ginkgo biloba L. Common Names: Maidenhair tree,
More informationOptimization Method for Determination of Carbofuran and Carboxin Residues in Cabbages by SPE and HPLC-UV
Chiang Mai J. Sci. 2007; 34(2) 227 Chiang Mai J. Sci. 2007; 34(2) : 227-234 www.science.cmu.ac.th/journal-science/josci.html Contributed Paper Optimization Method for Determination of Carbofuran and Carboxin
More informationExtended Application Note
Extended Application Note Comparison of 4 μm vs. 2.o Columns in Analysis of Drospirenone/Ethinyl Estradiol Tablet APP A-339 www.mtc-usa.com 1-732-578-1777 INTRODUCTION The formulation of drospirenone and
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF LACOSAMIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.3 (2011), 666-672 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR IN BULK AND ITS PHARMACEUTICAL FORMULATION V.Kalyan Chakravarthy*
More informationSubcritical Water Extraction of Polyphenolic Compounds from Terminalia chebula Fruits
Chiang Mai J. Sci. 2008; 35(1) FU-003 103 Chiang Mai J. Sci. 2008; 35(1) : 103-108 www.science.cmu.ac.th/journal-science/josci.html Contributed Paper Subcritical Water Extraction of Polyphenolic Compounds
More informationQualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution
Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution Application Note Food Testing Author Edgar Naegele
More informationARTENIMOLUM ARTENIMOL. Adopted revised text for addition to The International Pharmacopoeia
February 2012 ARTENIMOLUM ARTENIMOL Adopted revised text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications for Pharmaceutical
More informationDevelopment and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1736-1744, Oct-Dec 2013 Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GEFITINIB
More informationIJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet
More informationSimultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method
Asian Journal of Chemistry Vol. 22, No. 5 (2010), 3369-3375 Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(9):70-80 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Stability Indicating
More informationHIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE
Research Paper THE ASIAN JOURNAL OF HORTICULTURE Vol. 6 Issue 2 December, 2011 388-392 Article history: Received : 16.08.2011 Revised : 07.09.2011 Accepted : 04.10.2011 Effect of integrated nutrient management
More informationGlucosinolate biosynthesis as influenced by growth media and auxin in hairy root cultures of kale (Brassica oleracea var.
Emirates Journal of Food and Agriculture. 2016. 28(4): 277-282 doi: 10.9755/ejfa.2016-01-064 http://www.ejfa.me/ SHORT COMMUNICATION Glucosinolate biosynthesis as influenced by growth media and auxin in
More informationQuetiapine Tablets. Expert Committee Monographs Chemical Medicines 4 Reason for Revision Compliance
Quetiapine Tablets Type of Posting Revision Bulletin Posting Date 25 Sep 2015 Official Date 01 Nov 2015 Expert Committee Monographs Chemical Medicines 4 Reason for Revision Compliance In accordance with
More informationF. Al-Rimawi* Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, East Jerusalem. Abstract
JJC Jordan Journal of Chemistry Vol. 4 No.4, 2009, pp. 357-365 Development and Validation of Analytical Method for Fluconazole and Fluconazole Related Compounds (A, B, and C) in Capsule Formulations by
More informationResidue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016.
Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Quinoline Yellow This monograph was also published in: Compendium of Food Additive
More informationGLUCOSINOLATES IN KALE GENOTYPES FROM THE BLACKSEA REGION OF TURKEY
Articles A&EB GLUCOSINOLATES IN KALE GENOTYPES FROM THE BLACKSEA REGION OF TURKEY G. Sarıkamış 1, A. Balkaya 2, R.Yanmaz 1 Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara,
More informationA high-performance liquid chromatography (HPLC) method for determination of chlorogenic acid and emodin in Yinhuang Jiangzhi Tea
Journal of Hainan Medical University 2016; 22(12): 17-21 17 Journal of Hainan Medical University http://www.jhmuweb.net/ A high-performance liquid chromatography (HPLC) method for determination of chlorogenic
More information2D-LC as an Automated Desalting Tool for MSD Analysis
2D-LC as an Automated Desalting Tool for MSD Analysis Direct Mass Selective Detection of a Pharmaceutical Peptide from an MS-Incompatible USP Method Application Note Biologics and Biosimilars Author Sonja
More informationComparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation
Oriental Journal of Chemistry Vol. 24(2), 537-544 (2008) Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation P.N. DALVI,
More informationDevelopment, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method
ISSN: 0973-4945; CODEN ECJAO E- Chemistry http://www.e-journals.net 2012, 9(1), 340-344 Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by PLC Method V.
More informationDevelopment of Supercritical Fluid Extraction of Glabridin from Glycyrrhiza glabra
Development of Supercritical Fluid Extraction of Glabridin from Glycyrrhiza glabra On-line Number 939 Yun-Kyoung Cho, 1 Sang-Yun Lee, 1,2 Hyun-Seok Kim, 3 Jong-Hoon Ryu, 1 and Gio-Bin Lim 1 1 Department
More informationHyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.
International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik
More informationNovel approach to intensify the formation of the bioactive sulforaphane in cooked Brassica vegetables
Novel approach to intensify the formation of the bioactive sulforaphane in cooked Brassica vegetables Dr. Sameer Khalil Ghawi, University of Reading, UK Glucosinolates-myrosinase system in Brassica vegetables
More informationORAC Assay Kit KF A/ B 96/ 192 tests (96 well plate)
ORAC Assay Kit KF-01-004 A/ B 96/ 192 tests (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Assay protocol Pag. 4 Data analysis Pag. 8 References Pag. 9 Warranties and
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF ANTIRETROVIRAL DRUGS IN TABLET DOSAGE FORMS
Int. J. Chem. Sci.: 14(4), 2016, 2461-2466 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF ANTIRETROVIRAL DRUGS IN TABLET DOSAGE FORMS K. PARAMESWARA
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic
More informationProtein Cleavage Due to Pro-oxidative Activity in Some Spices
Protein Cleavage Due to Pro-oxidative Activity in Some Spices Sittiwat Lertsiri Department of Biotechnology Faculty of Science, Mahidol University Phayathai, Bangkok 10400 Thailand Kanchana Dumri Department
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
51 CHAPTER 2 SIMULTANEOUS ESTIMATION OF PIOGLITAZONE, GLIMEPIRIDE AND GLIMEPIRIDE IMPURITIES IN COMBINATION DRUG PRODUCT BY A VALIDATED STABILITY-INDICATING RP-HPLC METHOD 2.1 INTRODUCTION OF DOSAGE FORM
More informationAppendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water
Appendix A: Preparation of Media and Chemicals Preparation of Malt Extract Agar (MEA) Malt Extract Agar (MEA) weighing 13.44 g was dissolved in 400 ml of distilled water in an Erlenmeyer flask using a
More informationA HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE
Int. J. Chem. Sci.: 6(1), 2008, 441-446 A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR LERCANIDIPINE HYDROCHLORIDE S. APPALA RAJU, ARVIND B. KARADI and SHOBHA MANJUNATH HKES s College of Pharmacy,
More informationSupercritical Carbon Dioxide Extraction of Lignan from Cold-Press By-product of Sesame Seed
Supercritical Carbon Dioxide Extraction of Lignan from Cold-Press By-product of Sesame Seed Miyuki Date a, Siti Machmudah b, Mitsuru Sasaki a, Motonobu Goto b* a Graduate School of Science and Technology
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.1 (2011), 165-171 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR AND ITS PHARMACEUTICAL FORMULATION V. Kalyana Chakravarthy * and
More informationANTIOXIDANT ACTIVITY OF β-carboline DERIVATIVES
Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 68 No. 2 pp. 185ñ189, 2011 ISSN 0001-6837 Polish Pharmaceutical Society DRUG BIOCHEMISTRY ANTIOXIDANT ACTIVITY OF β-carboline DERIVATIVES RENATA FRANCIK
More informationINTERNATIONAL PHARMACOPOEIA MONOGRAPH ON LAMIVUDINE TABLETS
RESTRICTED INTERNATIONAL PHARMACOPOEIA MONOGRAPH ON LAMIVUDINE TABLETS DRAFT FOR COMMENT Please address any comments you may have on this document, by 12 July 2006, to Dr S. Kopp, Quality Assurance and
More informationRapid and sensitive UHPLC screening for water soluble vitamins in sports beverages
APPLICATION NOTE 21671 Rapid and sensitive UHPLC screening for water soluble vitamins in sports beverages Authors Jon Bardsley, Thermo Fisher Scientific, Runcorn, UK Keywords Vanquish Flex, Acclaim PolarAdvantage
More informationIdentification of phanerosporic acid in birch degraded by Phanerochaete chrysosporium
IRG/WP 03-10496 THE INTERNATIONAL RESEARCH GROUP ON WOOD PRESERVATION Section 1 Biology Identification of phanerosporic acid in birch degraded by Phanerochaete chrysosporium Michael D. Mozuch and Philip
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(4): 30-37 ISSN: 0976-8688 CODEN (USA): PSHIBD Development and validation of stability indicating RP-HPLC method for simultaneous
More informationEvaluation of the in vitro antioxidant potential of extracts obtained from Cinnamomum zeylanicum barks
Evaluation of the in vitro antioxidant potential of extracts obtained from Cinnamomum zeylanicum barks Lidiya Georgieva, Dasha Mihaylova Evaluation of the in vitro antioxidant potential of extracts obtained
More informationDetermination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry
Determination of β2-agonists in Pork Using Agilent SampliQ SCX Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry Application Note Food Safety Authors Chenhao Zhai Agilent
More informationPLANT SCIENCE MEETS FOOD SCIENCE: GENETIC EFFECTS OF GLUCOSINOLATE DEGRADATION DURING FOOD PROCESSING IN BRASSICA
PLANT SCIENCE MEETS FOOD SCIENCE: GENETIC EFFECTS OF GLUCOSINOLATE DEGRADATION DURING FOOD PROCESSING IN BRASSICA Kristin Hennig Thesis committee Thesis supervisor Prof. dr. ir. Martinus A. J. S. van Boekel
More informationA New RP-HPLC Method for the Simultaneous Estimation of Naltrexone Hydrochloride and Bupropion in Its Pure and Pharmaceutical Dosage Form
Human Journals Research Article July 2018 Vol.:12, Issue:4 All rights are reserved by A. Yasodha et al. A New RP-HPLC Method for the Simultaneous Estimation of Naltrexone Hydrochloride and Bupropion in
More informationAnalysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection
APPLICATION NOTE Liquid Chromatography Authors: Catharine Layton Wilhad M. Reuter PerkinElmer, Inc. Shelton, CT Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection Introduction Cannabis
More information