THE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL

Size: px
Start display at page:

Download "THE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL"

Transcription

1 THE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL

2 PROBLEM Do sports drinks have adverse effects on microflora cells?

3 HUMAN SYMBIONTS, PATHOGENS, AND FLORA More than 10% of your body mass is due to symbionts and pathogens Mostly Prokaryotic cells Some may cause diseases Also protect the body from foreign invaders Reinforce immune system, prevent allergies, and produce vitamins Lie within skin, saliva, and gastrointestinal tracts Break down abnormally large nutrients

4 E.COLI C600 Used in many cell/biochemical investigations Easy to manipulate in labs Normally found in the intestines of animals. Some are pathogenic, but most are harmless Large and diverse group of gram(-) bacteria Utilized in this study as a human cell model and as a model of pathogens E.coli was chosen for this experiment because of it s diversity, it lives in the intestinal tract of humans and many other mammals, and it serves as a common prokaryotic cell model.

5 GATORADE Very commonly used beverage amongst athletes and nonathletes Provides electrolytes Common uses include: During sports, Post-sport recovery, and as a common drink Thought to be non-toxic substance with no effects on body Ingredients: Contains water, sucrose (table sugar), dextrose, citric acid, natural flavor, sodium chloride (table salt), sodium citrate, monopotassium phosphate, and flavoring/coloring ingredients, and sucrose acetate isobutyrate.

6 PURPOSE To assess the effects of Gatorade on the survivorship of E.coli The human micro flora performs many functions vital to human health.

7 HYPOTHESES Null Hypothesis: Gatorade will not affect the survivorship of E.coli Alternative Hypothesis: Gatorade will significantly reduce the survivorship of E.coli

8 MATERIALS YEPD agar plates (1% Yeast Extract, 2% peptone, 2% dextrose) Sterile pipette tips Micropipettes Vortex Incubator Spreading turntable Spreader bar Sports Drink (Gatorade) Sterile capped test tubes with Sterile Dilution Fluid [SDF] (10mM KH2PO4, 10mM K2HPO4, 1mM MgSO4,.1mM CaCl2, 100mM NaCl) E.Coli C600

9 PROCEDURE 1.Bacteria (E.coli) were grown overnight in sterile LB media. 2. Samples of the overnight cultures were added to fresh media in a sterile sidearm flask. 3. The cultures were placed in an incubator (37 C) until a density of 50 Klett spectrophotometer units was reached. This represents a cell density of approximately 10^8 cells/ml. 4. The cultures were diluted in sterile fluid to a concentration of approximately 10^5 cells/ml. 5: LB agar plates(1% tryptone,.5% yeast extract, 1%NaCl) were prepared 6: Gatorade was pipetted into tubes containing SDF, to create the following concentrations: 0%, 1%, 10%, 25%, and 50%, yielding a final volume of 10 ml.

10 PROCEDURE (CONTINUED) Sterile Dilution Fluid Concentration Chart 0% 1% 10% 25% 50% 9.9 mlchange 9.8 ml 8.9 ml 7.4 ml 4.9 ml Microbe 0.1 ml 0.1 ml 0.1 ml 0.1 ml 0.1 ml Gatorade 0 ml 0.1 ml 1 ml 2.5 ml 5 ml Total Volume 10 ml 10 ml 10 ml 10 ml 10 ml 7. The cells were vortexed for even suspension. 8. After vortexing to evenly suspend cells, 0.1mL aliquots were removed from the tubes at time 10 minutes and 50 minutes and spread on YEPD agar plates (5 plates per concentration)

11 PROCEDURE (CONTINUED) 9. The plates were then incubated at 30 degrees for 48 hours. 10. The resulting colonies were counted. Each colony is assumed to have arisen from one cell.

12 Colonies The Effects of Sports Drinks on E.coli Survivorship P Value P Value - Less than 1x10E-6 0% 1% 10% 25% 50% Concentrations (10 Minutes) (50 Minutes)

13 DUNNETT S TEST T-Critical = Min Exposure Variable Concentration T-Value Interpretation 1% Gatorade 1.12 Not Significant 10% Gatorade 2.09 Not Significant 25% Gatorade 0.54 Not Significant 50% Gatorade 5.42 Significant 50 Min Exposure Variable Concentration T-Value Interpretation 1% Gatorade 0.46 Not Significant 10% Gatorade Significant 25% Gatorade 6.83 Significant 50% Gatorade Significant

14 ANALYSIS OF EXPOSURE TIME Did the duration of exposure have a significant effect? Ratio of Survivorship of Variables to Control (10 Min Exposure) 1% Gatorade 10% Gatorade 25% Gatorade 50% Gatorade Control (0% Gatorade) 96% 93% 102% 83% Ratios of Survivorship of Variables to Control (50 Min Exposure) 1% Gatorade 10% Gatorade 25% Gatorade 50% Gatorade Control (0% Gatorade) 101% 71% 81% 61%

15 CONCLUSIONS Generally, increased concentrations of Gatorade lowered the amount of E.coli cells that grew while some concentrations provided aid to growth Increased exposure did have a significant effect on the amount of surviving colonies. Higher concentrations of Gatorade could also be effective in reducing the amount of E.coli colonies that could survive

16 LIMITATIONS Limited number of concentrations Only 1 species test Only 2 exposure times During the testing, plating could have possibly been unsynchronized. Only one type of Sports Drink was used.

17 FURTHER STUDIES Different types of Sports Drinks More of a variety of concentrations Experiment on other types of cells

18 SOURCES

19 DATA RESULTS 0% Gatorade 1% Gatorade 10% Gatorade 25% Gatorade 50% Gatorade Amount of Colonies per plate (10 min Exposure) Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Average

20 DATA RESULTS (CONTINUED) 0% Gatorade 1% Gatorade 10% Gatorade 25% Gatorade 50% Gatorade Amount of Colonies per plate (50 min Exposure) Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Average

21 Colonies SURVIVORSHIP OF E.COLI The Effects of Sports Drinks on E.coli 0% 1% 10% 25% 50% Concentrations (10 Minutes) (50 Minutes)

22 DATA ANALYSIS (ANOVA)

The Effects of Alcohol and Nicotine on Microbial Flora. Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School

The Effects of Alcohol and Nicotine on Microbial Flora. Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School The Effects of Alcohol and Nicotine on Microbial Flora Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School Microbial Flora The internal and external flora has eukaryotic fungi, protists, and

More information

The Effects of Shampoo on Microbial Flora. Andrew Walker Grade 9 Central Catholic High School

The Effects of Shampoo on Microbial Flora. Andrew Walker Grade 9 Central Catholic High School The Effects of Shampoo on Microbial Flora Andrew Walker Grade 9 Central Catholic High School Shampoo Hair care product used to clean hair of unwanted build up Combined soap, water, and herbs to make hair

More information

Effects of Ethyl Alcohol on Microbial Survivorship. Tim Olson 9th Grade Central Catholic High School

Effects of Ethyl Alcohol on Microbial Survivorship. Tim Olson 9th Grade Central Catholic High School Effects of Ethyl Alcohol on Microbial Survivorship Tim Olson 9th Grade Central Catholic High School Ethyl Alcohol Pure alcohol or drinking alcohol Oldest recreational drug Alcohol intoxication Inhibits

More information

Hydrogen Peroxide Influence on Microbial Survivorship. Jacob Cebulak Central Catholic Pittsburgh Grade 9

Hydrogen Peroxide Influence on Microbial Survivorship. Jacob Cebulak Central Catholic Pittsburgh Grade 9 Hydrogen Peroxide Influence on Microbial Survivorship Jacob Cebulak Central Catholic Pittsburgh Grade 9 Problem Humans use excess hydrogen peroxide to clean wounds. The concentration used is often damaging

More information

By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA

By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA PROBLEM Saccharin is consumed by many humans on a day to day basis. Overconsumption of saccharin may

More information

Vitamin C and Ibuprofen Effects on Escherichia Coli. Timothy Leisenring Grade 11 Central Catholic High School

Vitamin C and Ibuprofen Effects on Escherichia Coli. Timothy Leisenring Grade 11 Central Catholic High School Vitamin C and Ibuprofen Effects on Escherichia Coli Timothy Leisenring Grade 11 Central Catholic High School Rationale for Experiment Commonly, ingested materials are investigated for effects on human

More information

Synergistic Effects of Vitamin B12 and Creatine on Microbes. Jake Rocchi CCHS, 11th grade 2nd year in PJAS

Synergistic Effects of Vitamin B12 and Creatine on Microbes. Jake Rocchi CCHS, 11th grade 2nd year in PJAS Synergistic Effects of Vitamin B12 and Creatine on Microbes Jake Rocchi CCHS, 11th grade 2nd year in PJAS Vitamin B12 Water-soluble vitamin Needed for proper functioning in brain, nervous system, and blood

More information

Antimicrobial Effects of Vinegar. Daniel Crawford Grade 9 Central Catholic High School

Antimicrobial Effects of Vinegar. Daniel Crawford Grade 9 Central Catholic High School Antimicrobial Effects of Vinegar Daniel Crawford Grade 9 Central Catholic High School Antibacterial Agents Humans interest in microbial growth/survivorship for over 100 years Many products/technologies

More information

The Effects of Reiki on Bacteria Survivorship. Jordan Ciccone Central Catholic High School

The Effects of Reiki on Bacteria Survivorship. Jordan Ciccone Central Catholic High School The Effects of Reiki on Bacteria Survivorship Jordan Ciccone Central Catholic High School Reiki Japanese spiritual healing practice meaning universal life energy. Training is done by a Reiki Master. Either

More information

Artificial Sweetener Effects on Microbial Flora. Thomas Peilert Central Catholic High School

Artificial Sweetener Effects on Microbial Flora. Thomas Peilert Central Catholic High School Artificial Sweetener Effects on Microbial Flora Thomas Peilert Central Catholic High School Problem Can the artificial sweetener Equal Original affect survivorship of Escherichia coli? Microbial Flora

More information

Chlorine and UV Effects on Bacteria Survivorship. Oscar Shaver Grade 9 Central Catholic High School

Chlorine and UV Effects on Bacteria Survivorship. Oscar Shaver Grade 9 Central Catholic High School Chlorine and UV Effects on Bacteria Survivorship Oscar Shaver Grade 9 Central Catholic High School Electromagnetic Spectrum Diagram showing the range of all types of frequencies of electromagnetic radiation

More information

By: Stephen Pfau Pittsburgh Central Catholic High School Grade 9

By: Stephen Pfau Pittsburgh Central Catholic High School Grade 9 By: Stephen Pfau Pittsburgh Central Catholic High School Grade 9 Everyday thousands of people take cold/flu medicine and pain relief medicine Two common medicines for cold/flu and pain are DayQuil (cold)

More information

Blair Bean Grade 9 Pittsburgh Central Catholic Highschool

Blair Bean Grade 9 Pittsburgh Central Catholic Highschool Blair Bean Grade 9 Pittsburgh Central Catholic Highschool Variety of organisms that live in and on the body Skin Gastrointestinal tracts Nose Pharynx Mouth Conjunctiva Provide moisture, nourishment, and

More information

BY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL

BY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL BY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL INTRODUCTION Chicken is one of the most consumed meats in the world, though can pose health risks (salmonella). Salmonella was thought only

More information

NIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION

NIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION NIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION MUSIC Elements: pitch, rhythm, timbre, texture, and dynamics Different types of music can exclude and include certain

More information

Water. Nutrition Facts Serving Size 20 fl oz (591 ml) Servings Per Container 1. Amount Per Serving Calories 0 Calories from Fat 0

Water. Nutrition Facts Serving Size 20 fl oz (591 ml) Servings Per Container 1. Amount Per Serving Calories 0 Calories from Fat 0 Water Serving Size 20 fl oz (591 ml) Servings Per Container 1 Calories 0 Calories from Fat 0 Sodium 0mg 0% Total Carbohydrate 0g 0% Sugars 0g Not a significant source of other nutrients. Ingredients: PURIFIED

More information

Calculations Key Drink Label Cards

Calculations Key Drink Label Cards Calculations Key Drink Label Cards Recommended Uses for Drink Label Cards 1. Display Drink Label Cards with sugar cubes representing the teaspoons of sugar in each container. Be sure to also include MyPlate

More information

ALL OF THESE DRINKS: WHAT DO YOU THINK? HOW MUCH SUGAR IS IN YOUR DRINK?

ALL OF THESE DRINKS: WHAT DO YOU THINK? HOW MUCH SUGAR IS IN YOUR DRINK? LESSON 3: WINNING THE BALANCE GAME - FATS AND SUGARS, EVALUATING FOOD ADS, AND IDENTIFYING PORTION SIZES WHAT DO YOU THINK? HOW MUCH SUGAR IS IN YOUR DRINK? USE THE SUGAR CUBES ON THE TABLE TO CREATE A

More information

Calculations Key Drink Label Cards

Calculations Key Drink Label Cards Calculations Key Drink Label Cards Instructor Tips for Drink Label Cards 1. Display Drink Label Cards with sugar cubes representing the teaspoons of sugar in each container. Display should also include

More information

The Effects of Spray Sunscreen on MG63 Cancer Cell Lines. Anthony Williams Pittsburgh Central Catholic High School Grade 11

The Effects of Spray Sunscreen on MG63 Cancer Cell Lines. Anthony Williams Pittsburgh Central Catholic High School Grade 11 The Effects of Spray Sunscreen on MG63 Cancer Cell Lines Anthony Williams Pittsburgh Central Catholic High School Grade 11 Sunscreen Meant to protect against harmful UV rays that can be carcinogenic Comes

More information

BACTERIAL EXAMINATION OF WATER

BACTERIAL EXAMINATION OF WATER BACTERIAL EXAMINATION OF WATER The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing,

More information

Biological Consulting Services

Biological Consulting Services Biological Consulting Services of North Florida/ Inc. May 13, 2009 Aphex BioCleanse Systems, Inc. Dear Sirs, We have completed antimicrobial efficacy study on the supplied Multi-Purpose Solution. The testing

More information

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE Translated English of Chinese Standard: GB4789.30-2016 www.chinesestandard.net Buy True-PDF Auto-delivery. Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.30-2016

More information

METABOLIC INJURY TO BACTERIA AT LOW TEMPERATURES

METABOLIC INJURY TO BACTERIA AT LOW TEMPERATURES METABOLIC INJURY TO BACTERIA AT LOW TEMPERATURES ROBERT P. STRAKA AND J. L. STOKES Western Regional Research Laboratory,' Albany, California Received for publication January 19, 1959 The death of bacteria

More information

FORMULATION, SENSORY EVALUATION AND NUTRIENT ANALYSIS OF PRODUCTS WITH ALOE VERA

FORMULATION, SENSORY EVALUATION AND NUTRIENT ANALYSIS OF PRODUCTS WITH ALOE VERA WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Joy K et al. Volume 2, Issue 6, 5321-5328. Research Article ISSN 2278 4357 FORMULATION, SENSORY EVALUATION AND NUTRIENT ANALYSIS OF PRODUCTS WITH ALOE

More information

Bill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11

Bill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11 Bill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11 The technical term for saltiness in aquatic environments is halinity, from the fact that halides, or chloride, are the most abundant anions in

More information

Laboratory Protocol. November 2017 Version 3. Henrik Hasman, Yvonne Agersø and Lina M Cavaco (DTU Food)

Laboratory Protocol. November 2017 Version 3. Henrik Hasman, Yvonne Agersø and Lina M Cavaco (DTU Food) Laboratory Protocol Validation of selective MacConkey agar plates supplemented with 1 mg/l cefotaxime for monitoring of ESBL- and AmpCproducing E. coli in meat and caecal samples November 2017 Version

More information

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009.

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009. Translated English of Chinese Standard: GB5009.259-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD GB OF THE PEOPLE S REPUBLIC OF CHINA National food safety standard Determination

More information

Recipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones)

Recipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones) Protocol: 300 ml Yeast culture preparation Equipment and Reagents needed: Autoclaved toothpicks Shaker Incubator set at 30 C Incubator set at 30 C 60 mm 2 sterile petri dishes Autoclaved glass test tubes

More information

CHAPTER 4 IMMUNOLOGICAL TECHNIQUES

CHAPTER 4 IMMUNOLOGICAL TECHNIQUES CHAPTER 4 IMMUNOLOGICAL TECHNIQUES Nitroblue Tetrazolium Chloride (NBT) Reduction test NBT reduction test was evaluated by employing the method described by Hudson and Hay,1989 based upon principle that

More information

Indigenous fermented milk products: A microbiological study in Bhagalpur town

Indigenous fermented milk products: A microbiological study in Bhagalpur town RESEARCH ARTICLE FOOD SCIENCE RESEARCH JOURNAL Volume 4 Issue 1 April, 2013 24-28 Indigenous fermented milk products: A microbiological study in Bhagalpur town A.R. NIGAM, R.P. SAH AND MD. IRSHAD ALAM

More information

Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal

Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Article Summary (In short - What is your article about Just 2 or 3 lines) Category: Bacillus sp produce

More information

HARMONISED PHARMACOPOEIA DEHYDRATED CULTURE MEDIA FOR SUPPORTING REGULATORY COMPLIANCE AVAILABLE NOW P O RTF O LIO.

HARMONISED PHARMACOPOEIA DEHYDRATED CULTURE MEDIA FOR SUPPORTING REGULATORY COMPLIANCE AVAILABLE NOW P O RTF O LIO. DEHYDRATED CULTURE MEDIA FOR ENHANCED P O RTF O LIO AVAILABLE NOW HARMONISED PHARMACOPOEIA SUPPORTING REGULATORY COMPLIANCE A Neogen Company THE GATEWAY TO MICROBIOLOGY INTRODUCTION Harmonised Pharmacopoeia;

More information

Microbiological Methods V-A- 1 SALMONELLA SPECIES PRESUMPTIVE AND CONFIRMATION TESTS

Microbiological Methods V-A- 1 SALMONELLA SPECIES PRESUMPTIVE AND CONFIRMATION TESTS Microbiological Methods V-A- 1 PRESUMPTIVE AND CONFIRMATION TESTS PRINCIPLE SCOPE Enrichment and selective procedures are used to provide a reasonably sensitive, definitive and versatile means of qualitatively

More information

ANTIBACTERIAL TOOTHPASTE: DO NOT SWALLOW

ANTIBACTERIAL TOOTHPASTE: DO NOT SWALLOW ANTIBACTERIAL TOOTHPASTE: DO NOT SWALLOW Sarah McCuaig BACKGROUND, PURPOSE, HYPOTHESES Market statistics indicate a significant increase in the use of antibacterial products in North American households.

More information

S. aureus NCTC 6571, E. coli NCTC (antibiotic

S. aureus NCTC 6571, E. coli NCTC (antibiotic ISO Sensitivity Test Agar Code: KM1204 A semi-defined nutritionally rich sensitivity medium. It is composed of specially selected peptones with a small amount of glucose, solidified with a very pure agar

More information

Selective Growth Media for Differentiation and Detection of Escherichia Coli and Other Coliforms

Selective Growth Media for Differentiation and Detection of Escherichia Coli and Other Coliforms Page 1 of 5 Page 1 of 5 Return to Web Version Selective Growth Media for Differentiation and Detection of Escherichia Coli and Other Coliforms By: Jvo Siegrist, AnalytiX Volume 8 Article 4 E. coli and

More information

SCREENING LACTIC ACID BACTERIA FOR ANTIMICROBIAL COMPOUND PRODUCTION K. KHALISANNI, K. LEE HUNG

SCREENING LACTIC ACID BACTERIA FOR ANTIMICROBIAL COMPOUND PRODUCTION K. KHALISANNI, K. LEE HUNG SCREENING LACTIC ACID BACTERIA FOR ANTIMICROBIAL COMPOUND PRODUCTION K. KHALISANNI, K. LEE HUNG Department of Microbiology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam,

More information

Calibration Protocols

Calibration Protocols Calibration Protocols (1)OD 600 Reference point LUDOX Protocol Materials: 1ml LUDOX CL-X (provided in kit) ddh20 (provided by team) 96 well plate, black with clear flat bottom preferred (provided by team)

More information

YOUR. drink Choose Water!

YOUR. drink Choose Water! rethink YOUR drink Choose Water! Let s Not -coat It! -sweetened beverages (SSBs) are beverages with added sugar These drinks include soda pop, juice/fruit drinks, sweetened teas/coffees, flavored waters,

More information

YOUR. drink Choose Water!

YOUR. drink Choose Water! rethink YOUR drink Choose Water! Let s Not -coat It! -sweetened beverages (SSBs) are beverages with added sugar These drinks include soda pop, juice/fruit drinks, sweetened teas/coffees, flavored waters,

More information

Microbes as Agents of Infectious Disease

Microbes as Agents of Infectious Disease Microbes as Agents of Infectious Disease Normal Flora Virulence and Pathogenicity Toxicity vs. Invasiveness WE ARE NOT ALONE! We are outnumbered. The average human contains about 10 trillion cells. On

More information

BACTERIAL EXAMINATION OF WATER

BACTERIAL EXAMINATION OF WATER BACTERIAL EXAMINATION OF WATER The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing,

More information

Laboratorios CONDA, S.A. Distributed by Separations

Laboratorios CONDA, S.A. Distributed by Separations Culture Media as on Pharmacopoeia 7.3, Harmonized Method for Microbiological Examination of non sterile products -FORMULATIONS Buffered sodium chloride-peptone solution ph 7.0 Cat. Nº 1401 Potassium dihydrogen

More information

Microbes as Agents of Infectious Disease

Microbes as Agents of Infectious Disease Microbes as Agents of Infectious Disease Normal Flora Virulence and Pathogenicity Toxicity vs. Invasiveness WE ARE NOT ALONE! We are outnumbered. The average human contains about 10 trillion cells. On

More information

Understanding probiotics and health

Understanding probiotics and health Understanding probiotics and health Gemma Laws MSc Student Microbiology and Immunology Department The gut microbiota The name given to the total microbial population living in our intestine Bacteria, fungi,

More information

Flavour: Red fruits, peach and passion fruit, orange, lime and grapefruit Package size: 1.7g Pharmaceutical form: Powder

Flavour: Red fruits, peach and passion fruit, orange, lime and grapefruit Package size: 1.7g Pharmaceutical form: Powder Flavour: Red fruits, peach and passion fruit, orange, lime and grapefruit Package size: 1.7g Pharmaceutical form: Powder Isotonic sports drink with added vitamins Energy Mineral is a multipurpose isotonic

More information

Williams Lab Recipes ANTIBIOTICS

Williams Lab Recipes ANTIBIOTICS Williams Lab Recipes ANTIBIOTICS 1000x Ampicillin (sodium salt) 100mg/ml recipe 1. Measure out 1 g of Ampicillin tri hydrate 2. Add Milli-Q H2O to 10 ml 3. Add ~.1 g of NaOH pellets (half pellet or more

More information

A Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance

A Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance A Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance Abstract Outbreaks of Salmonella contamination of peanut butter have become a serious food safety concern for

More information

CHAPTER III MATERIALS AND METHODS

CHAPTER III MATERIALS AND METHODS CHAPTER III MATERIALS AND METHODS An investigation entitled Standardization and storage of Ready to serve and candy of ber fruit (Zizyphus mauritiana Lamk.) was carried out in the Department of Horticulture,

More information

Hassan Pyar Kok-Khiang Peh *

Hassan Pyar Kok-Khiang Peh * Isolation of probiotics Lactobacillus acidophilus from commercial yoghurt Hassan Pyar Kok-Khiang Peh * School of Pharmaceutical Sciences, University Sains Malaysia, 11800 Minden, Penang, Malaysia. Telephone

More information

Coenzyme Q-10 Effects on Mammalian Cell Behavior. Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013

Coenzyme Q-10 Effects on Mammalian Cell Behavior. Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013 Coenzyme Q-10 Effects on Mammalian Cell Behavior Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013 An Overview of Stem Cells Unspecialized cells capable of renewing themselves through

More information

Screening and isolation of microbial contaminants from carbonated and non-carbonated soft drinks of Delhi

Screening and isolation of microbial contaminants from carbonated and non-carbonated soft drinks of Delhi International Journal of Emerging Trends in Science and Technology IC Value: 76.89 (Index Copernicus) Impact Factor: 4.219 DOI: https://dx.doi.org/10.18535/ijetst/v4i5.04 Screening and isolation of microbial

More information

Effect of Spray Tank Materials on Anti-Bacterial Activities of Sodium Hypochlorite

Effect of Spray Tank Materials on Anti-Bacterial Activities of Sodium Hypochlorite Effect of Spray Tank Materials on Anti-Bacterial Activities of Sodium Hypochlorite Jean M. Bonasera and Steven V. Beer Section of Plant Pathology and Plant Microbe Interaction School of Integrative Plant

More information

Survival of Aerobic and Anaerobic Bacteria in

Survival of Aerobic and Anaerobic Bacteria in APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,

More information

Professor Diane Hilker

Professor Diane Hilker Professor Diane Hilker I. Exp. 19: Water Analysis for Fecal Contamination II. Exp. 20: Quantitative Analysis of H 2 O Purpose: To learn & perform the 3 stage standard H 2 O analysis test for fecal contamination

More information

A selective medium for the enumeration of coliforms in water and milk by the membrane filter method.

A selective medium for the enumeration of coliforms in water and milk by the membrane filter method. ENDO BROTH A selective medium for the enumeration of coliforms in water and milk by the membrane filter method. Code: KM6612 Tryptone 5.00 Bacto Peptone 5.00 Soy Peptone 10.00 Yeast Extract 1.50 Lactose

More information

Radiation Inactivation of Microbes in Fresh Vegetables

Radiation Inactivation of Microbes in Fresh Vegetables Radiation Inactivation of Microbes in Fresh Vegetables ZENAIDA M. DE GUZMAN Supervising Science Research Specialist Philippine Nuclear Research Institute PHILIPPINE NUCLEAR RESEARCH INSTITUTE PNRI Co-60

More information

INTRODUCING YOUR GUT BACTERIA

INTRODUCING YOUR GUT BACTERIA INTRODUCING YOUR GUT BACTERIA Microflora Intestinal flora 1.5 kg We would die with 5 years of birth if we did not have them as we would not develop a proper immune system 1000 species and 5000 strains

More information

Stool bench. Cultures: SARAH

Stool bench. Cultures: SARAH Stool bench The bacteria found in stool are representative of the bacteria that are present in the digestive system (gastrointestinal tract). Certain bacteria and fungi called normal flora inhabit everyone's

More information

G/LITRE 5.0 g KOH g 0.5 g 0.05 g 0.01 g MgS047H20 NaCl CaCl2

G/LITRE 5.0 g KOH g 0.5 g 0.05 g 0.01 g MgS047H20 NaCl CaCl2 A P P E N D IX -V III COMPOSITION OF USED MEDIA AND CHEMICAL REAGENTS 1. NITROGEN FREE BROMOTHYMOL BLUE (NFB) MEDIUM Dobereiner et al (1976) Same media was also used to check the effect of temperature

More information

Evaluation of Antibacterial Effect of Odor Eliminating Compounds

Evaluation of Antibacterial Effect of Odor Eliminating Compounds Evaluation of Antibacterial Effect of Odor Eliminating Compounds Yuan Zeng, Bingyu Li, Anwar Kalalah, Sang-Jin Suh, and S.S. Ditchkoff Summary Antibiotic activity of ten commercially available odor eliminating

More information

ß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose

ß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose ß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose JILLIAN CLARK, JACQUIE HUDSON, ROBIN MAK, CHRISTA McPHERSON, AND CARMEN TSIN Department of Microbiology

More information

Comparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor

Comparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor Comparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor M. G. Sanal Kumar, S. Nandakumar, B. Bini & Arya Raj R. S. P.G. & Research Department of Zoology,

More information

BACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION. SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE

BACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION. SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE BACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE slaramo1@fau.edu 772 242 2525 Spread Plate Method This is the easiest and most common plating

More information

ASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS

ASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS Lucrări ştiinńifice Zootehnie şi Biotehnologii, vol. 41(1) (2008), Timişoara ASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS TESTAREA INFLUENłEI SISTEMELOR

More information

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety

International Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X

More information

Questionnaires on Functional beverages

Questionnaires on Functional beverages Questionnaires on Functional beverages Prepared by: SPRIM Date: 19 th July 2016 Interview/ questionnaire on the functional beverages and nutritional products Experts detail (Please fill in the information

More information

Nutrition Facts. More Information G2 - FRUIT PUNCH. 12 fl oz. Per 1 Bottle (355 ml) serving Caffeine: 0mg Phosphorus: 36mg Common Allergens: None

Nutrition Facts. More Information G2 - FRUIT PUNCH. 12 fl oz. Per 1 Bottle (355 ml) serving Caffeine: 0mg Phosphorus: 36mg Common Allergens: None G2 - FRUIT PUNCH 12 fl oz Serving Size 1 Bottle (355 ml) Amount Per Serving Calories 30 Total Fat 0g Sodium 160mg Potassium 45mg Total Carbohydrate 8g Sugars 7g Protein 0g % Daily Value* 0% 7% 1% 3% Not

More information

Microplate Nutrient Analysis: NH 4 +, NO 3 -, PO 4

Microplate Nutrient Analysis: NH 4 +, NO 3 -, PO 4 Microplate Nutrient Analysis: NH 4 +, NO 3 -, PO 4 3+ Making Solutions: It is helpful to make the reagents and the concentrated (100 or 50 ppm) stock solution the day before running samples. When you are

More information

Experiment 2 Introduction

Experiment 2 Introduction Characterization of Invertase from Saccharomyces cerevisiae Experiment 2 Introduction The method we used in A Manual for Biochemistry I Laboratory: Experiment 7 worked well to detect any created reducing

More information

Animal Nutrition. What s in a soybean? Activity 1: Soybean nutrients

Animal Nutrition. What s in a soybean? Activity 1: Soybean nutrients Animal Nutrition name: date: class: Welcome to the GrowNextGen Animal Nutrition Plug-and-Play Lesson. This lesson will take approximately three class periods (120 minutes total) and will enhance and expand

More information

APPENDIX I. I )! i. 0.3 g Magnesium sulfate (Hydrated) g Calcium carbonate g Ferrous sulfate g Agar-agar - 20.

APPENDIX I. I )! i. 0.3 g Magnesium sulfate (Hydrated) g Calcium carbonate g Ferrous sulfate g Agar-agar - 20. APPENDIX I 1. Starch casein agar medium Medium was used for isolation, morphological studies, maintaining the cultures and for antitumor antibiotic production from the soil and river sediments isolates.

More information

THE ANTIMICROBIAL ACTIVITY OF DIFFERENT MOUTHWASHES IN MALAYSIA. Mat Ludin, C.M., and Md Radzi, J.

THE ANTIMICROBIAL ACTIVITY OF DIFFERENT MOUTHWASHES IN MALAYSIA. Mat Ludin, C.M., and Md Radzi, J. Malaysian Journal of Medical Sciences, Vol. 8, No. 2, July 2001 (14-18) ORIGINAL ARTICLE THE ANTIMICROBIAL ACTIVITY OF DIFFERENT MOUTHWASHES IN MALAYSIA Mat Ludin, C.M., and Md Radzi, J. Department of

More information

National Standard of the People s Republic of China. National food safety standard. Determination of pantothenic acid in foods for infants and

National Standard of the People s Republic of China. National food safety standard. Determination of pantothenic acid in foods for infants and National Standard of the People s Republic of China GB 5413.17 2010 National food safety standard Determination of pantothenic acid in foods for infants and young children, milk and milk products Issued

More information

Helping You Manage the Side Effects of Your Cancer Therapy

Helping You Manage the Side Effects of Your Cancer Therapy Helping You Manage the Side Effects of Your Cancer Therapy Many of us have watched loved ones go through the challenges of being treated for cancer. Now that you ve been diagnosed, you may have concerns

More information

Comparative Efficacy of Peroxyacetic Acid and Sodium Hypochlorite Bleach against Enterobacteria, E. coli and Yeasts Molds on Cherries

Comparative Efficacy of Peroxyacetic Acid and Sodium Hypochlorite Bleach against Enterobacteria, E. coli and Yeasts Molds on Cherries Comparative Efficacy of Peroxyacetic Acid and Sodium Hypochlorite Bleach against Enterobacteria, E. coli and Yeasts Molds on Cherries Purpose The primary purpose of this study was to determine whether

More information

Petrifilm Enterobacteriaceae Count Plate

Petrifilm Enterobacteriaceae Count Plate Petrifilm Enterobacteriaceae Count Plate Interpretation Guide 1 2 1 2 Enterobacteriaceae count = 1 It is easy to count Enterobacteriaceae colonies on a Petrifilm Enterobacteriaceae count plate. A red indicator

More information

Chemotaxis Response in Caenorhabditis elegans to an Olfactory Repellent Paired with Nicotine

Chemotaxis Response in Caenorhabditis elegans to an Olfactory Repellent Paired with Nicotine Chemotaxis Response in Caenorhabditis elegans to an Olfactory Repellent Paired with Nicotine Amanda Bigness and Dr. Robert Grammer Belmont University Recent studies in C. elegans, a model organism with

More information

PARENTERAL PREPARATIONS

PARENTERAL PREPARATIONS PARENTERAL PREPARATIONS INTRODUCTION Parenteral (Gk, para enteron, beside the intestine) dosage forms differ from all other drug dosage forms, because they are injected directly into body tissue through

More information

Interpretation Guide. Enterobacteriaceae Count Plate

Interpretation Guide. Enterobacteriaceae Count Plate Interpretation Guide The 3M Petrifilm Enterobacteriaceae Count Plate is a sample-ready-culture medium system that contains modified Violet Red Bile Glucose (VRBG) nutrients, a cold-watersoluble gelling

More information

Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples

Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples Background The VACUETTE Urine CCM tube is for the collection, transport and storage of urine samples

More information

FUEL YOUR COMPETITIVE SPIRIT NUTRITION AND HYDRATION GUIDELINES FOR SERIOUS ATHLETICS

FUEL YOUR COMPETITIVE SPIRIT NUTRITION AND HYDRATION GUIDELINES FOR SERIOUS ATHLETICS FUEL YOUR COMPETITIVE SPIRIT NUTRITION AND HYDRATION GUIDELINES FOR SERIOUS ATHLETICS ELEVATE YOUR ENERGY Carbohydrates Are King Just like automobiles, our bodies require energy to perform. Carbohydrates

More information

1.1. Gelatinizing Plates Prepare plates by covering surface with 0.1% Gelatin solution:

1.1. Gelatinizing Plates Prepare plates by covering surface with 0.1% Gelatin solution: 1. Preparation of Feeder Layers And SNL Stocks 1.1. Gelatinizing Plates Prepare plates by covering surface with 0.1% Gelatin solution: Plate Size (cm) 3 2 6 4 10 9 15 18 Amount of Gelatin (ml) Swirl the

More information

Microbes and Food 4/18/14

Microbes and Food 4/18/14 Ques%on(about(the(E.#coli#data.( Group(Projects(and(the(proposal:( Laboratory(Reports((wri%ng(project(#1)( Tuesday:( Start(microbes(in(food(experiment( Begin(phase(contrast(Microscopy( Thursday:( Analysis(and(observa%on(for(microbes(in(food(exercise.(

More information

Count. Enterobacteriaceae. Interpretation Guide. 3M Food Safety 3M Petrifilm Enterobacteriaceae Count Plate

Count. Enterobacteriaceae. Interpretation Guide. 3M Food Safety 3M Petrifilm Enterobacteriaceae Count Plate 3M Food Safety 3M Petrifilm Enterobacteriaceae Count Plate Enterobacteriaceae Count Interpretation Guide This guide familiarizes you with results on 3M Petrifilm Enterobacteriaceae Count Plates. For more

More information

2-Deoxyglucose Assay Kit (Colorimetric)

2-Deoxyglucose Assay Kit (Colorimetric) 2-Deoxyglucose Assay Kit (Colorimetric) Catalog Number KA3753 100 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...

More information

Diffusion, Osmosis and Active Transport

Diffusion, Osmosis and Active Transport Diffusion, Osmosis and Active Transport 1 of 25 Boardworks Ltd 2012 2 of 25 Boardworks Ltd 2012 Substances spread out by diffusion 3 of 25 Boardworks Ltd 2012 Particles in solutions and in gases move around

More information

Scholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis

Scholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2012, 2 (4):612-618 (http://scholarsresearchlibrary.com/archive.html) Purification and characterization

More information

Microbiological Quality of Non-sterile Products Culture Media for Compendial Methods

Microbiological Quality of Non-sterile Products Culture Media for Compendial Methods Microbiological Quality of Non-sterile Products Culture Media for Compendial Methods The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. Culture Media for Compendial Methods

More information

Appendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water

Appendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water Appendix A: Preparation of Media and Chemicals Preparation of Malt Extract Agar (MEA) Malt Extract Agar (MEA) weighing 13.44 g was dissolved in 400 ml of distilled water in an Erlenmeyer flask using a

More information

Lab #9. Introduction. Class samples:

Lab #9. Introduction. Class samples: Lab #9 Introduction Food-borne illness is largely caused by the presence of bacteria in red meat. However, much of these harmful bacteria can be destroyed and prevented by sanitation and safe cooking practices.

More information

Aperto Cell Lysis and Protein Solubilization Users Manual

Aperto Cell Lysis and Protein Solubilization Users Manual Aperto Cell Lysis and Protein Solubilization Users Manual Revision 2 THIS MANUAL APPLIES TO THE FOLLOWING PRODUCTS: 3A8600 Aperto, 5X Cell Lysis Buffer. 20mL 3A8610 Aperto, 5X Cell Lysis Buffer. 100mL

More information

Product Use HPSC-CC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Product Use HPSC-CC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. HPSC-derived Cardiomyocyte Cells (HPSC-CC) Catalog #6240 Cell Specification Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity

More information

APPENDIX Reagents. Appendix. Alsever s solution Citric acid 0.55g Sodium citrate 8.0g D-glucose 20.5g Sodium Chloride 4.2g

APPENDIX Reagents. Appendix. Alsever s solution Citric acid 0.55g Sodium citrate 8.0g D-glucose 20.5g Sodium Chloride 4.2g APPENDIX Reagents Alsever s solution Citric acid 0.55g Sodium citrate 8.0g D-glucose 20.5g Sodium Chloride 4.2g 1000 ml Barritt s reagent A α-naphthol 5.0g ethanol 95.0 ml α-naphthol was dissolved in ethanol

More information

Int.J.Curr.Microbiol.App.Sci (2014) 3(3):

Int.J.Curr.Microbiol.App.Sci (2014) 3(3): ISSN: 2319-7706 Volume 3 Number 3 (2014) pp. 668-674 http://www.ijcmas.com Original Research Article Antibiotic resistant pattern of urinary tract infection causing Escherichia coli isolated from diabetic

More information

Perfect Empowered Drinking Water

Perfect Empowered Drinking Water uses a unique 15-step purification process to remove contaminants. All that's left is a revitalizing, clean, refreshing taste. Trace minerals and electrolytes are infused back in and enhanced with oxygen

More information

Microbial Quality Analysis of Milk and Flavoured Milk Products from Local Vendors in Vellore

Microbial Quality Analysis of Milk and Flavoured Milk Products from Local Vendors in Vellore Microbial Quality Analysis of Milk and Flavoured Milk Products from Local Vendors in Vellore Aditya Sood*, Ridhi Sood, Abhijit Kumar, Gaganjot Kaur, Candy Sidhu Assistant Professor, Chandigarh University,

More information

What s in your fluid bottle?

What s in your fluid bottle? What s in your fluid bottle? Last month I looked at why we sweat, what factors affect our sweat rate and the effect of dehydration through sweating on both physical and mental performance. As a result

More information

TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays.

TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays. TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS Objective To make a serial dilution for use in dose/response bioassays. Background The idea behind a bioassay is that the test organism will respond in

More information