Stor Jabatan Mikrobiologi
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1 A. MEDIA BIL BAHAN KIMIA Stor Jabatan Mikrobiologi KUANTITI SEMASA 1 1-Naphtylamine, approx. 98 % 100 g 1 2 2,2 -Azino-bis (3-ethylbenzothiazoline ml 1 3 Albumin fraction V 25 g 1 4 2,3,5-triphenyltetrazolium chloride 5 g 1 UNIT KUANTITI 5 Agar bacteriological 1 6 Bacto casitone 7 Brain heart infusion agar 500 g 7 8 Blood agar base 500 g 3 9 Bacto Pepone 2 10 Chlortetracycline hydrochloride 5 g 1 11 DRBC agar 12 DNase test agar 13 D(+)glucose monohydrate 14 Endo agar 15 Endo agar base 16 Endo broth 500 g 3 17 Eosin Methylene blue Agar 500g 6 18 Gentamycin sulphate 10 ml 6 19 Hydrogen peroxide 35 % 1 liter 4 20 Hektoen Enteric Agar 21 Hanks Balanced salt solution 1X 1 liter 1 22 Lab lemco broth 23 Lactobacili MRS Agar 200 g, 1 500g 1 24 Leifsonagar 25 LB broth 500 g 2 26 Litmus milk 500 g 8 27 Lactobacili MRS broth 500 g 2 28 mfc Muller-kauffmann broth base tetrathionat novobiocine enrichment 500 g 3 29 broth 30 Mannitol salt agar 500 g 2 31 Mac-conkey broth 500 g 3 32 Mueller-hinton broth
2 33 M.R.V.P medium 500 g 3 34 Mac-conkey agar 500 g 3 35 Nitrate broth 36 p-nitrophenyl phosphate Di ris salt 100 g 1 37 Nutrient gelatine 0 38 Nutrient agar 500 g 5 39 Nutrient broth 500 g 6 40 Phenol:chloroform:isoamyl Phenol:chloroform:isoamyl alcohol alcohol 2524:1 saturated 100 ml 1 41 with 10mm tris, ph 8.0, 1 mm EDTA 100 ml 1 42 Phenylalanine agar 500 g 2 43 Peptone water 44 Pseudomonas agar base 45 Phenylethyl alcohol agar 46 Potato Dextrose agar 200 g 1 47 Potato dextrose broth 250 g 2 48 Sulfonic acid 100 ml 1 49 Starch agar 50 Sabouraud dextrose agar 500 g 7 51 Skim milk powder 500 g 4 52 SIM medium 500 g 2 53 Streptococcus broth 54 Simmons citrate agar 500 g 2 55 Sabouraud dextrose liquid medium 56 Tissue culture medium 100 g 4 57 Triple sugar iron agar 500 g 2 58 Tryptone 59 Thiogly collate medium U.S.P 60 Todd-hewitt broth 61 T.C.B.S TCBS agar cholera (B/S) medium (Thiosulphate citrate bile salts sucrose 62 agar) 63 Tryptone soya broth 500 g 3 64 Tryptone soya agar 500 g 5 65 Urea broth base 66 VRBD-agar 67 Wilkins-chalgren agar
3 68 X.L.D medium 69 Yeast mannitol agar 500 g 2 70 Yeast Extract B. BAHAN KIMIA BIL BAHAN KIMIA UNIT KUANTITI SEMASA KUANTITI 1 Ammonium amidosulfonate 100 g 1 2 Ammonium molybdate (tetrahydrate) 250 g 1 3 Agarose 100 g 1 4 Agarose, standard, Low Electroendosmosis (EEO) 100 g 1 5 Ammonium Sulphate 1 kg 2 6 Ampicillin 5 g 1 7 Ammonium sulphamate 8 Ammonium di-hydrogen phosphate 500 g 2 9 Ammonium Iron (II) Sulphate Hexahydrate 500 g 4 10 Ammonium Persulphate 11 Ammonium Iron (III) sulfat (dodecahydrate) 12 Bromocresol Purple Sodium Salt 10 g 1 13 Bromophenol Blue-(Water Soluble) 10 g 1 14 Bromophenol Blue-Water Soluble Indicator 10 g 2 15 Bovine Albumin Fraction V ( Powder) 10 g 1 16 Coomassie Brilliant Blue G-250 Baker 25 g 1 17 Boric Acid (Granular) 1 kg 1 18 Cobalt (II)chloride (hexahydrate) 19 Crystal violet 25 g 4 20 Calcium carbonate 21 Cooper (II) sulphate 500 g 3 22 Celite Hyflo Supercel 1 kg 1 23 Carbol Fuchsin 25 g 7 24 Carbol Fuchsin (gram) 100 ml 2 25 Calcium Sulfate dihydrate precip. extra pure 1 kg 1 26 Calcium Chloride-dihydrate 1 kg 1 27 Creatine ~ monohydrate 25 g 1 28 Cesium Chloride 100 g 1 29 Chloroform 4 liter 1 30 N, N-Dimethy 1-1,4-phenylenediamine, 97% 5 g 1 31 Diphenylamine extrapure AR 100 g 1 32 D(+) glucose 1 kg 1 33 Dithiothreitol 1 g 1 34 Ethidium bromide 5 g 1 35 Follin & ciocateus phenol reagent 500 ml 1 36 Fuchsin basic 25 g 1 37 Glyceryl Tributyrate 100 ml 3
4 38 Glycine 39 Isoamyl Alcohol extrapure AR 500 ml 2 40 Iron (III) Citrate (Trihydrate) 41 Immersion Oil 100 ml 1 42 Iodine Re sublimed 100 g 3 43 Iron (II) sulphate 44 Kresolrot 25 g 2 45 L-lysine hydrochloride 100 g 1 46 Lactophenol blue 100 ml 4 47 Lysol 1 liter 2 48 Lactose (Monohydrate) 49 Litmus Indicator 25 g 2 50 Litmus A.R 25 g 1 51 Lactophenol Blue Solution 100 ml 4 52 Lowen Stein Jensen Medium Base 53 Lactose 1-hydrate 500 g 2 54 D(-)-mannitol 55 Methylene blue 100 g 2 56 Magnesium carbonate (light) 57 Methylrot 100 g 1 58 Methy len blau 100 g Mercaptoethanol pure 100 ml 1 60 Magnesium Sulphate-heptahydrate 1 kg 1 61 Magnesium Chloride-hexahydrate 1 kg 1 62 Methyl Cellulose 63 Methyl Red ph Indicator 100 g 1 64 Methyl Red Indicator 100 g 1 65 Methyl Orange 100 g 2 66 Methyl Orange 100 g 1 67 Manganese (II) Sulphate (Monohydrate) 68 Maltose ~ Monohydrate 250 g 1 69 Malachite Green 25 g, 3 25 g 1 70 Methyl Red 25 g 1 Methylene Blue 25 g 3 71 Methylene Blue 100 g 1 72 Methyl Green 25 g, 1 15 g 1 73 Methyl Green (Ethyl Green) 5 g 1 74 Nessler s Reagent 500 ml Naphthol 100 g 1 76 Nigrosine (Alcohol Soluble) 25 g Naphtylamine 78 Nigrosine (water soluble) 25 g 2 79 N,N-Dimethyl-1,4-phenylenediamine oxalate 5 g 1 80 Polyethylene glycol 8000 crystalline powder 1 kg 1
5 81 Potassium Sodium Tartrate 500 g 2 82 Potassium Hydrogen Sulfate for analysis 500 g 2 83 Potasium dihydrogen phosphate (anhydrous) 1 kg 1 84 Potasium Chloride 480 ml 2 85 Pyridoxine Hydrochloride ( Vitamin B 6 ) 25 g 2 86 Phenolphthalein 100 g 1 87 di-potassium hydrogen phosphate (anhydrous) 500 g 2 88 Potassium Nitrate 1 kg 2 89 Penicillin G Potassium Salt - 90 Penicillin-G (Benzylpenicilin) Sodium Salt - 91 Phenol-Chloroform-Isoamyl alcohol mixture 100 ml 1 92 Pottasium acetate 93 Phenol 94 Phenol red 25 g 1 95 Polyethylene glycol g 2 96 p-rosolic Acid 25 g 1 97 Resazurin, certified 5 g 1 98 Sodium Nitrate for analysis 99 Sodium Nitrite 1 kg Sodium Sulphate 1 kg g 1 Sodium Arsenate dibasic heptahydrate, minimum 98.0 % 100 g Sodium Carbonate-anhydrous 103 Sodium Thiosulfate-5-hydrate 1 kg di-sodium Hydrogen Phosphate (anhydrous) 1 kg Di-Sodium Tetraborate-decahydrate 500 g Sodium dihydrogen Phosphate (anhydrous) 1 kg Sodium Hydroxide Pellet 1 kg Tri-Sodium Citrate (Dihydrate) 1 kg Sulfuric Acid 2.5 liter Silica Gel Blue 1 kg Sulfanilic Acid anhydrous A.R 100 g Sodium Acetate anhydrous GR for analysis 1 kg Starch Soluble 114 Safranin O 25 g Sucrose ~ D(+)Saccharose 1 kg di-sodium Hydrogen Phosphate Anhydrous AnapuR 500 g TBE Solution 1 liter N, N, N, N Tetramethylethylenediamine pure 100 ml Tris buffer (for MFG only) 6.5 ml Thiamine hydrochloride 100 g Trichlorfon 250 mg 1
6 122 TE buffer ph ml TAE buffer 25 X solution 1 liter Zinc Chloride 125 Zinc Sulphate (Heptahydrate) 126 Zink (logam), serbuk 127 Acetic Acid Glacial 2.5 liter Acid Clean 25 liter Hydrogen Peroxide 30 % 130 Xylene 2.5 liter Iso.amyl acetate 2.5 liter liter Methanol 1 liter Glycerol anhydrous 2.5 liter Ethanol Teracun (Denatured Ethanol 95 %) 25 liter 1 Diethyl ether for analysis 5 liter Diethyl ether for analysis 2.5 liter Ethyl Alchol 99.8 % (v/v)-undenatured 2.5 liter Ethyl Alcohol 95 % (v/v)-denatured 2.5 liter Ethyl Alcohol ~ 95 % v/v min 2.5 liter Iso propyl Alcohol 4 liter Ethyl Alcohol 2.5 liter 2 3 liter, Absolute Alcohol 99.7 % 500 ml Ethyl Alcohol 95 % v/v min (Denatured) 25 liter 5
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