EXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN
|
|
- Rosemary Hutchinson
- 6 years ago
- Views:
Transcription
1 BIOLOGIA 2001, 47 (1&2), PP ISSN EXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN *HAMAD ASHRAF, IKRAM UL HAQ, AND JAVED IQBAL Biotechnology Research Laboratory, Department of Botany, Govt. College, Lahore, Pakistan. ABSTRACT: The present study is concerned with the optimization of conditions for the extraction of alpha amylase from fermented wheat bran. Phosphate buffer (ph 7.5) was used as an extractant, bran to extractant ratio of 1:10 was found to be optimum for extraction of maximum enzyme. There was a correlation between agitation intensity, time of agitation and temperature for the extraction of extracellular & thermostable alpha amylase. Time duration of agitation (for extraction of enzyme) was decreased as the agitation intensity was increased from 100 rpm. The maximum enzyme was extracted (3600 IU/g/min) when the agitation intensity was maintained at 200 rpm for one hour. The extraction of enzyme was increased after every 5 o C rise in temperature and reached optimum (3850 IU/g/min) at 65 o C. Keywords: Alpha amylase, Bacillus, Fermentation, Phosphate buffer, Solid state, INTRODUCTION The enzyme, alpha amylase, which converts starches into oligosaccharides is the most important product obtained for human needs through microbial sources. A large number of industrial processes in the area of textile, food biotechnology, pharmaceutical and detergent utilize the thermostable alpha amylase to great extent (Nigam & Sing, 1995). The enzymes from Bacillus subtilis were found to be more thermostable; therefore, it can be preferred for industrial production of alpha amylase (Ludikhuyze et al., 1996). Solid-state fermentation holds tremendous potential for the production of alpha amylase. It can be of special interest in those processes where crude fermented product may be used directly as enzyme source. Different agricultural byproducts such as wheat bran, rice bran, cottonseed meal, soybean meal etc., can be used for their exploitation as substrates by solid-state fermentation (Ramesh & Lonsane, 1990; Ramesh & Lonsane, 1991; Babu & Satyanarayana, 1995). The enzyme produced by solid substrate cultivation extracellularly and remains attached with the fermentation medium (Ramadas et al., 1995). For the commercial production of enzyme as well as for its maximum activity, it is necessary to separate the maximum enzyme from fermented wheat bran (Ramesh & Lonsane, 1989). The extraction from dried bran by buffers not only yielded higher enzyme titre but also show its maximum activity. Phosphate buffer (ph 7.5) was found to be most suitable for the extraction of alpha amylase from the fermented wheat bran (Haq et al., 1998). The present study is concerned with the optimisation of conditions for the extraction of extra-cellular alpha amylase from the fermented wheat bran.
2 BIOLOGIA HAMAD ASHRAF, ET AL 48 MATERIALS AND METHODS Organism The mutant strain of Bacillus licheniformis GCUCM-30 (a thermophilic strain, enzyme active at o C) isolated and tested for alpha amylase production in this laboratory was used for present study. The organism was maintained at nutrient broth starch agar slants. The strains were stored in paraffin oil containing nutrient starch agar medium. Inoculum Preparation Vegetative inoculum was used in present studies. Fifty ml of inoculum medium containing (%w/v) nutrient broth 1.0, soluble starch 1.0, NaCl 0.5, CaCl in 100 ml of phosphate buffer was transferred to each of 250 ml cotton plugged Erlenmeyer flask and sterilized at 15lb pressure (121 C) for 15 minutes. After cooling at room temperature, a loopful of bacteria was aseptically transferred to each flask. The flasks were then rotated in the rotary shaking incubator (200 rpm) at 40 C for 24 hours. Fermentation Technique Solid-state fermentation technique was employed. Ten gram solid substrate (wheat bran 7.0g & cotton seed meal 3.0 g) was transferred to 250 ml Erlenmeyer flask and moistened with 10 ml phosphate buffer. The flasks were sterilized in the autoclave and cooled at room temperature. The flasks were inoculated with one ml of vegetative inoculum and incubated at 40 C for 48h. After 48h 100ml of phosphate buffer was transferred to each flask and then rotated in the rotary incubator shaker at 200 rpm for one hour. After one hour the fermented bran was filtered and filtrate was used for the estimation of alpha amylase. All the experiments were run parallel in triplicates. Enzyme Assay Alpha amylase estimation was carried out according to the method of Rick & Stegbauer (1974). The enzyme solution at ph 7.5 was incubated at 60 o C using 1% soluble starch solution. The reducing sugars were measured by adding 3,5-dinitro salicylic acid reagent, boiling for 5 min, cooling and measuring the O.D at 540 nm in the spectrophotometer (Model CECIL CE7200) against maltose as standard. One unit of activity is equivalent to that amount of enzymes, which in 10 minutes librates reducing group from 1% Lintner s soluble starch corresponding to 1 mg maltose hydrate. Statistical Analysis Treatment effects were compared by the method of Snedecor & Cochrane (1980). RESULTS AND DISCUSSION The separation of alpha amylase from fermented wheat bran is very essential for its commercial application. Phosphate buffer (ph 7.5) was used as an extractant because previously (Haq et al., 1998) it was found to be the best extractant and stabilizer of alpha amylase. The ratio of extractant to fermented bran is a critical factor for maximum separation of enzyme. Different ratios (1:2.5-1:20) of bran to extractant were evaluated and the maximum extraction of alpha amylase (3500 IU/g/min) was obtained when bran to extractant ratio was 1:10. As the amount of extractant was increased, there was gradual reduction in the extraction of enzyme. It might be due to the fact that high ratios of bran to extractant acted as a hindrance for proper agitation and separation of
3 Vol. 47(1&2) ALPHA AMYLASE EXTRACTION FROM FERMENTED BRAN 49 enzyme (Ramadas et al., 1995). At low ratios of bran to extractant the extraction of alpha amylase was insignificant. It might be due to the fact that at low ratio, the amount of extractant was insufficient to extract the attached enzyme from fermented wheat bran as the extractant becomes more concentrated. Therefore, 1:10 ratio of bran to extractant was selected for subsequent experiments. The agitation intensity and its time duration play a very critical role in the extraction of attached enzyme from fermented wheat bran. For this purpose the relationship between agitation intensity and time of agitation was investigated (Fig., 2). It was observed that with increase in the agitation intensity, the time duration for the extraction of enzyme was decreased. The extraction of enzyme was found to be optimum (3600 IU/g/min) after one hour when the enzyme was extracted at 200 rpm. Further increase in the agitation intensity was found to be insignificant. Below 200 rpm the insignificance of the results might be due to the fact that agitation was not proper, thus causing insufficient separation of enzyme from fermented bran. Padmanabhan et al. (1992) have reported that the separation of enzyme from fermented bran was increased as the time of agitation was increased from 60 to 120 min. However, in this study the maximum separation of the enzyme (3850 IU/g/min) was achieved only after 60 min. Thus, this finding is more encouraging than the work reported by Padmanabhan et al. (1992) because it takes lesser time for the extraction of enzyme; it would lead towards not only safe energy but also economy of the country. Thus, agitation intensity of 200 rpm for one hour was selected for extraction of enzyme from fermented wheat bran. The temperature also plays a very critical role in the extraction of thermostable enzyme because the stability and activity of alpha amylase is very sensitive to temperature (Babu & Satyanarayana, 1995). It was observed that there was a correlation between temperature and separation of enzyme from fermented wheat bran (Fig. 3). The extraction of enzyme was increased with increase in the temperature after every 5 o C rise in temperature and reached maximum at 65 o C. It might be due to the fact that at high temperature, the permeability of the mash was increased and hence the leaching of the enzyme from fermented bran was also increased (Prescott & Dunn, 1987). Further increase in the temperature caused decrease in the extraction of the enzyme. It might be due to the fact that denaturing of the enzyme started at high temperature (Ludikhuyze et al., 1996) that resulted decrease in the activity of alpha amylase. Therefore, agitation intensity of 200 rpm at 65 o C for one hour was optimized for the preparation of crude alpha amylase at industrial level. CONCLUSION From the present study it was concluded that there was a correlation between agitation intensity, time of agitation and temperature for the extraction of extracellular and thermostable alpha amylase. By using the optimum conditions for extraction of alpha amylase from fermented bacterial bran, energy and economy of the country can be saved which makes the present study more encouraging.
4 BIOLOGIA HAMAD ASHRAF, ET AL 50 REFERENCES Babu, K.P. & Satyanarayana, T., Alpha-amylase production by thermophilic Bacillus coagulans in solid state fermentation. Process Biochem. 30(4): Haq, I., Ashraf, H., Zahara, R. & Qadeer, M. A., Biosynthesis of alpha amylase by Bacillus subtilis GCB-12 using agricultural by products as substrates. Biologia, 44(1&2): Ludikhuyze, L., De-Cordt, S., Weemaes, C., Hendrickx, M. & Tobback, P., Kinetics for heat and pressuretemperature inactivation of Bacillus subtilis alpha amylase. Food Biotechnol. 10(2): Nigam, P. & Sing, D., Enzyme and microbial system involved in starch processing. Enzyme Microb. Technol., 17: Padmanabhan, S., Ramakrishna, M., Lonsane, B.K. & Kishnaiah, M.M., Enhanced leaching of product at elevated temperature: Alpha amylase produced by Bacillus licheniformis M 27 in solidstate fermentation system. Lett. Appl. Microbiol., 15 (6): Prescott, S. & Dunn, A., Industrial microbiology. 4 th Ed. CBS Publishers and Distributors, New Delhi, India. pp: Ramadas, M., Holst, O. & Mattiasson, B.,1995. Extraction and purification of alpha amylase produced by solid state fermentation with Aspergillus niger. Biotechnol. Tech., 9 (12): Ramesh, M.V. & Lonsane, B.K., Purification of thermostable alpha amylase produced by Bacillus licheniformis M27 under solid state fermentation. Process Biochem., 24 (5): Ramesh, M.V. & Lonsane, B.K., Critical importance of moisture contents of the medium in alpha amylase production by Bacillus licheniformis M27 in a solid state fermentation system. Appl. Microbiol. Biotechnol., 33 (5): Ramesh, M.V. & Lonsane, B.K., Regulation of alpha amylase production in Bacillus licheniformis M 27 by enzyme end products in submerged fermentation and its overcoming in solid state fermentation system. Biotechnol. Lett., 13 (5): Rick, W. & Stegbauer, H. P., α- Amylase measurement of reducing groups. In H.V. Bergmeyer (Ed.), Methods of Enzymatic Analysis, 2 nd Ed., Vol. 2, Academic Publisher. Snedecor, G. W. & Cochrane, W. G., Statistical Methods, 7 th Edition. Ames, Iowa: Iowa State University Press. ISBN
5 Enzyme activity (IU/g/min) Vol. 47(1&2) ALPHA AMYLASE EXTRACTION FROM FERMENTED BRAN Amount of extractant (ml) IU/g/min Figure 1: Selection of bran to extractant ratio for the extraction of alpha amylase from fermented bran Each value is an average of three replicates. Y bars indicated the standard error. Agitation intensity (rpm) Figure 2: Selection of time and agitation intensity for the extraction of alpha amylase from fermented bran Each value is an average of three replicates. Y bars indicated the error.
6 Enzyme activity (IU/g/min) BIOLOGIA HAMAD ASHRAF, ET AL Temperature (C) Figure 3: Effect of temperature on the extraction of alpha amylase from fermented bacterial bran Each value is an average of three replicates. Y bars indicated the error.
EFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-110
BIOLOGIA 21, 47 (1&2), PP 59 65 ISSN 6 396 EFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-11 SIKANDER ALI 1, IKRAM-UL-HAQ 1 AND JAVED IQBAL 2 1 Biotechnology Research
More informationScreening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal
Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Article Summary (In short - What is your article about Just 2 or 3 lines) Category: Bacillus sp produce
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 211, 1 (3):124-129 ISSN: 2248 9215 Production of Alkaline Protease by Bacillus subtilis (MTCC7312) using Submerged
More informationTECHNIQUE FOR IMPROVED PRODUCTION OF 3,4 DIHYDROXY PHENYL L-ALANINE BY ASPERGILLUS ORYZAE
Pak. J. Bot., 39(2): 623-627, 2007. TECHNIQUE FOR IMPROVED PRODUCTION OF 3,4 DIHYDROXY PHENYL L-ALANINE BY ASPERGILLUS ORYZAE SIKANDER ALI AND IKRAM-UL-HAQ Institute of Industrial Biotechnology, Government
More informationINFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and
INFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and SESAMUM OIL CAKE as SUBSTRATES S M Gopinath, Department of Biotechnology, Acharya Institute of Technology,
More informationEffect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste
Biotechnological Communication Biosci. Biotech. Res. Comm. 8(1): 78-83 (2015) Effect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste Rupali R. Deshmukh and N. N. Vidhale*
More informationBiosynthesis of Citric Acid by Locally Isolated Aspergillus niger Using Sucrose Salt Media
OnLine Journal of Biological Sciences 1 (4): 178-181, 2001 Asian Network for Scientific Information 2001 Biosynthesis of Citric Acid by Locally Isolated Aspergillus niger Using Sucrose Salt Media Sikander
More informationStudy on moisture content of substrate (Sesamum oil cake and Rice chaff) on the yield of Citric acid
Int.J.Curr.Microbiol.App.Sci (14) 3(5): 138-144 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-776 Volume 3 Number 5 (14) pp. 138-144 http://www.ijcmas.com Original Research
More informationScholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2012, 2 (4):612-618 (http://scholarsresearchlibrary.com/archive.html) Purification and characterization
More informationComparative Study of Citric Acid Production from Annona reticulata and Its Peel with Effect of Alcohol as a Stimulant
International Journal of Advanced Biotechnology and Research(IJBR) ISSN 0976-2612, Online ISSN 2278 599X, Vol5, Issue3, 2014, pp346-352 http://www.bipublication.com Comparative Study of Citric Acid Production
More informationAspergillus foetidus BY AQUEOUS TWO PHASE
33 CHAPTER 3 PARTIAL PURIFICATION OF TANNASE FROM Aspergillus foetidus BY AQUEOUS TWO PHASE EXTRACTION AND ITS CHARACTERIZATION 3.1 INTRODUCTION Partial purification of proteins in general and tannase
More informationNovel technique for microbial production of 3,4-Dihydroxy Phenyl L- alanine by a mutant strain of Aspergillus oryzae
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.5 No.2, Issue of August 15, 2002 2002 by Universidad Católica de Valparaíso -- Chile Received December 21, 2001 / Accepted June 4, 2002 RESEARCH
More informationProduction of Thermostable and Ca +2 Independent α-amylases from Halphilic Bacteria
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 12, Number 2 (2016) pp. 153-159 Research India Publications http://www.ripublication.com Production of Thermostable and Ca
More informationEffect of starch containing organic substrates on alpha amylase production in Bacillus strains
African Journal of Microbiology Research Vol. 6(45), pp. 7285-7291, 27 November, 2012 Available online at http://www.academicjournals.org/ajmr DOI: 10.5897/AJMR12.1814 ISSN 1996-0808 2012 Academic Journals
More informationCorresponding author: Shalini Singh,
CELLULOSE CHEMISTRY AND TECHNOLOGY POTENTIAL OF CHEAP CELLULOSIC RESIDUE AS CARBON SOURCE IN AMYLASE PRODUCTION BY ASPERGILLUS NIGER SH-2 FOR APPLICATION IN ENZYMATIC DESIZING AT HIGH TEMPERATURES SHALINI
More informationEffect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S.
Available online at www.sciencedirect.com APCBEE Procedia 00 (2012) 000 000 Conference title Effect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S. Chuenjit
More informationAmylase Production from Potato and Banana Peel Waste
Int.J.Curr.Microbiol.App.Sci (213) 2(11): 41-414 ISSN: 2319-776 Volume 2 Number 11 (213) pp. 41-414 http://www.ijcmas.com Original Research Article Amylase Production from Potato and Banana Peel Waste
More informationImproving thermostable α amylase production by bacillus RB 4
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2014, 4(4):136-140 ISSN: 2248 9215 CODEN (USA): EJEBAU Improving thermostable α amylase production by bacillus
More informationJOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.625, ISSN: , Volume 2, Issue 11, December 2014
COMPARISON OF ACTIVITY OF PECTINASE USING AGRICULTURAL WASTE SUBSTRATES A STUDY M.P.KUSUMA* DR M.V.V.CHANDANA LAKSHMI** *Assistant Professor, RBVRR College of Pharmacy, Osmania University, India **Associate
More informationFermentation Medium Optimization for the Biosynthesis of Protease by Penicillium chrysogenum in Shake Flasks
Pakistan J. Zool., vol. (), pp. 9-73,. Fermentation Medium Optimization for the Biosynthesis of Protease by Penicillium chrysogenum in Shake Flasks IKRAM-UL-HAQ*, HAMID MUKHTAR AND HINA UMBER Institute
More informationSCREENING AND PRODUCTION OF α-amylase FROM ASPERGILLUS NIGER USING ZERO VALUE MATERIAL FOR SOLID STATE FERMENTATION
Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 975-1491 Vol 5, Issue 1, 13 Research Article SCREENING AND PRODUCTION OF α-amylase FROM ASPERGILLUS NIGER USING ZERO
More informationAcid Protease Production by Fungi Used in Soy3bean Food
APPLIED MICROBIOLOGY, May 1974, p. 906-911 Copyright 1974 American Society for Microbiology Vol. 27, No. 5 Printed in U.S.A. Acid Protease Production by Fungi Used in Soy3bean Food Fermentation HWA L.
More informationMedia Optimization Studies for Enhanced Production of Serratiopeptidase
Media Optimization Studies for Enhanced Production of Serratiopeptidase from Bacillus Licheniformis (NCIM ) Manasi J. Wagdarikar*, Anagha M. Joshi, Amir A. Shaikh SCES s Indira College of Pharmacy, Tathawade,
More informationProduction of Cellulase from Aspergillus fumigatus Under Submerged and Solid State Fermentation Using Agricultural Waste
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Production of Cellulase from Aspergillus fumigatus Under and Solid State Using Agricultural Waste P. Shobana and N.
More informationIsolation and Screening of Citric Acid Producing Aspergillus Spp and Optimisation of Citric Acid Production by Aspergillus Niger S-6
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-issn: 2319-2402,p- ISSN: 2319-2399.Volume 9, Issue 3 Ver. I (Mar. 2015), PP 19-23 www.iosrjournals.org Isolation and
More informationOptimization of saccharification conditions of prebiotic extracted jackfruit seeds
Paper Code: fb005 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Optimization of saccharification conditions of prebiotic extracted jackfruit seeds Sininart Chongkhong *, Bancha
More informationIsolation and Screening of Starch Hydrolising Bacteria and its Effect of Different Physiological. Parameters on Amylase Enzyme Activity
, pp: 79-83 NOVEMBER-2015 Research Article (Open access) Isolation and Screening of Starch Hydrolising Bacteria and its Effect of Different Physiological Parameters on Amylase Enzyme Activity Prerana Min*,
More informationOPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE
Int. J. Chem. Sci.: 8(2), 2010, 909-913 OPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE T. SATHISH a and N. Y. S. MURTHY * Department of Biotechnology, Malla Reddy Engineering College, HYDERABAD (A.P.)
More informationINTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY
[Ravish, 2(2): Feb., 2013] ISSN: 2277-9655 IJESRT INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY Isolation And Characterization Of Proteolytic Bacteria And Its Protease Himani Ravish
More informationEffect of metal ions on amylase production using Bacillus subtilis isolated from soil of Almora District, Uttarakhand, India
Available online at www.ijpab.com Sirohi, R. and Prakash, V. Int. J. Pure App. Biosci. 3 (4): 37-41 (2015) ISSN: 2320 7051 ISSN: 2320 7051 Int. J. Pure App. Biosci. 3 (4): 37-41 (2015) INTERNATIONAL JOURNAL
More informationPartial characterization and optimization of alkaline Amaylase from Bacillus spp. from Lonar Crater
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Partial characterization and optimization of alkaline Amaylase from Bacillus spp. from Lonar Crater Tambekar DH, Chandurkar
More informationExploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste
Int. J. of Life Sciences, 2016, Vol. 4 (3): 394-400 ISSN: 2320-7817 eissn: 2320-964X RESEARCH ARTICLE Exploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste Vidhale
More informationProduction and Optimization of Protease from Aspergillus niger and Bacillus subtilis using Response Surface Methodology
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 2, Issue 7 (Nov. Dec. 2016), PP 01-07 Production and Optimization of Protease from Aspergillus niger and Bacillus subtilis
More informationIsolation and screening of different isolates of Aspergillus for amylases production
ISPUB.COM The Internet Journal of Microbiology Volume 7 Number 1 Isolation and screening of different isolates of Aspergillus for amylases production V Morya, D Yadav Citation V Morya, D Yadav.. The Internet
More informationScreening, Isolation and Characterization of Amylase Producing Bacteria and optimization for Production of Amylase
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 4, Issue 2 (Mar. - Apr 2018), PP 50-56 www.iosrjournals.org Screening, Isolation and Characterization of Amylase Producing
More informationOPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL DESIGN
http://www.rasayanjournal.com Vol.4, No.1 (2011), 159-164 ISSN: 0974-1496 CODEN: RJCABP OPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL
More informationShruthi SD et al. IRJP 2012, 3 (8) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY
INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article PRODUCTION OF α-amylase FROM ASPERGILLUS FLAVUS UNDER SOLID STATE FERMENTATION WITH OPTIMUM CONDITIONS Mamatha
More informationAmylase Production from Solid State Fermentation and Submerged Liquid Fermentation by Aspergillus niger
BCSIR Available online at www.banglajol.info Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012 Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by Aspergillus niger G.
More informationEffect of Fermentation Parameters on Extra Cellular Tannase Production by Lactobacillus plantarum MTCC 1407
ISSN: 973-95; CODEN ECJHAO E- Chemistry http://www.e-journals.net 9, (), 979-9 Effect of Fermentation Parameters on Extra Cellular Tannase Production by Lactobacillus plantarum MTCC 17 KANNAN NATARAJAN
More informationScreening of Nutritional Parameters for the Production of Protease from Aspergillus Oryzae
ISSN: 0973-4945; CODEN ECJHAO E-Journal of Chemistry http://www.e-journals.net Vol. 4, No. 2, pp 208-215, April 2007 Screening of Nutritional Parameters for the Production of Protease from Aspergillus
More informationChapter OPTIMIZATION OF CONDITIONS FOR THE PRODUCTION OF ALKALINE PROTEASES
Chapter OPTIMIZATION OF CONDITIONS FOR THE PRODUCTION OF ALKALINE PROTEASES ~th the objective of obtaining high yield of alkaline proteases, factors lim influencing the production by the selected strains
More informationEffect of incubation temperature on production of amylase in various agroindustrial wastes by alkaliphilic actinobacteria
Bioscience Discovery, 8(3): 521-527, July - 2017 RUT Printer and Publisher Print & Online, Open Access, Research Journal Available on http://jbsd.in ISSN: 2229-3469 (Print); ISSN: 2231-024X (Online) Research
More informationExtraction of Enzymes from Potato Peels Substrate using Bacillus subtilis
ISSN: 2319-7706 Volume 4 Number 8 (2015) pp. 451-458 http://www.ijcmas.com Original Research Article Extraction of Enzymes from Potato Peels Substrate using Bacillus subtilis Ashish Joshi 1 *, M.N. Dabhi
More informationIsolation and Screening of Amylase Producing Fungi
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 783-788 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.604.098
More informationSolid State Fermentation (SSF) for the Production of Sophorolipids from Starmerella bombicola NRRL Y using glucose, wheat bran and oleic acid
Solid State Fermentation (SSF) for the Production of Sophorolipids from Starmerella bombicola NRRL Y-17069 using glucose, wheat bran and oleic acid Vishal J. Parekh and Aniruddha B. Pandit * Department
More informationStudies on Glucose Isomerase from a Streptomyces Species
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 1976, P. 489-493 Copyright ) 1976 American Society for Microbiology Vol. 32, No. 4 Printed in U.S.A. Studies on Glucose Isomerase from a Streptomyces Species
More informationProduction and stabilization of amylases from Aspergillus niger
Mycosphere Production and stabilization of amylases from Aspergillus niger Monga M, Goyal M *, Kalra KL and Soni G Department of Biochemistry and Department of Microbiology, Punjab Agricultural University,
More informationComparative analysis on immobilized cells of Aspergillus oryzae and Bacillus cereus in production of amylase by solid state and submerged fermentation
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2013, 3(3):178-183 ISSN: 2248 9215 CODEN (USA): EJEBAU Comparative analysis on immobilized cells of Aspergillus
More informationBioremediation of textile azo dyes by newly isolated Bacillus sp. from dye contaminated soil
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 13, Number 2 (2017) pp. 147-153 Research India Publications http://www.ripublication.com Bioremediation of textile azo dyes
More informationChapter 8. Optimization of growth. conditions favoring maximum. lipase production by selected. isolates
Chapter 8 Optimization of growth conditions favoring maximum lipase production by selected isolates Microbial lipases are mostly produced in submerged culture (Ito et al., 2001) but solid state fermentation
More informationCHAPTER 3 OPTIMIZATION OF PROCESS PARAMETERS FOR PROTEASE PRODUCTION IN SOLID STATE FERMENTATION
76 CHAPTER 3 OPTIMIZATION OF PROCESS PARAMETERS FOR PROTEASE PRODUCTION IN SOLID STATE FERMENTATION 3.1 INTRODUCTION Solid State Fermentation (SSF) is defined as the cultivation of microorganisms on moist
More informationLactic acid production from rice straw using plant-originated Lactobacillus rhamnosus PN04
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(5):590-594 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Lactic acid production from rice straw using plant-originated
More informationContinental J. Microbiology 4: 1-7, 2010 ISSN: Wilolud Journals, 2010
Continental J. Microbiology : - 7, ISSN: - 6 Wilolud Journals, http://www.wiloludjournal.com AMYLOLYTIC PROPERTIES OF FUNGI ASSOCIATED WITH SPOILAGE IN BREAD Okoko, F.J and Ogbomo, O. Microbiology Department,
More informationRecipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones)
Protocol: 300 ml Yeast culture preparation Equipment and Reagents needed: Autoclaved toothpicks Shaker Incubator set at 30 C Incubator set at 30 C 60 mm 2 sterile petri dishes Autoclaved glass test tubes
More informationOptimization for Production and Partial Purification of Laccase from Ash Gourd Peels
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 2 (2017) pp. 997-1003 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2017.602.112
More informationEffect of Starch on the Inactivation of Amylase in Starch-Containing Foods
Food Sci. Technol. Res., 19 (6), 989 993, 2013 Note Effect of Starch on the Inactivation of Amylase in Starch-Containing Foods Kazuo Koyama 1*, Jinji Shono 1, Hiromu Taguchi 1, Akira Toriba 2 and Kazuichi
More informationWorld Journal of Pharmaceutical and Life Sciences WJPLS
wjpls, 2018, Vol. 4, Issue 1, 80-84 Research Article ISSN 2454-2229 Rajarajan et al. WJPLS www.wjpls.org SJIF Impact Factor: 4.223 PRODUCTION AND CHARACTERIZATION OF AMYLASE FROM ASPERGILLUS NIGER Dr.
More informationProduction of Microbial Lipids by Yarrowia Lipolytica
Romanian Biotechnological Letters Vol. 2, No. 6, 215 Copyright 215 University of Bucharest Printed in Romania. All rights reserved ORIGINAL PAPER Production of Microbial Lipids by Yarrowia Lipolytica Received
More informationResearch Article ISSN Vol 2/Issue 4/Oct-Dec 2012 PRAGYA RATHORE*, PRATIK SHAH, HARSHPREET CHANDOK, SATYENDRA PATEL
THE PHOSPHATE SOLUBILIZING POTENTIAL OF CITROBACTER FREUNDII UNDER VARIOUS PHYSICOCHEMICAL CONDITIONS PRAGYA RATHORE*, PRATIK SHAH, HARSHPREET CHANDOK, SATYENDRA PATEL Sanghvi Institute of Management &
More informationSolid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues
Research Article Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues Vasudeo Zambare R&D Laboratory, Sequence Biotech Pvt. Ltd., Wadivarhe, Tal- Igatpuri, Dist-
More informationProduction of lipase from Aspergillus oryzae (T4) isolate by solidstate. Aspergillus oryza (T4)
Production of lipase from Aspergillus oryzae (T4) isolate by solidstate fermentation Aspergillus oryza (T4) Thikra A. Mustafa Shatha S.AL-Azawi* Khulood W.AL-Samarraei** Biology Department/ Collage of
More informationBACILLUS SUBTILIS: A POTENTIAL SALT TOLERANT PHOSPHATE SOLUBILIZING BACTERIAL AGENT
Int. J. LifeSc. Bt & Pharm. Res. 2014 V S Patil, 2014 Research Paper ISSN 2250-3137 www.ijlbpr.com Vol. 3, No. 2, April 2014 2014 IJLBPR. All Rights Reserved BACILLUS SUBTILIS: A POTENTIAL SALT TOLERANT
More informationOptimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation
African Journal of Biochemistry Research Vol. 4(3), pp. 73-8, March 21 Available online at http://www.academicjournals.org/ajbr ISSN 1996-778 21 Academic Journals Full Length Research Paper Optimization
More informationDepartment of Microbiology, Ekiti State University, P.M.B. 5363, Ado-Ekiti, Nigeria
Sustainable Food Production Submitted: 2017-12-15 ISSN: 2624-876X, Vol. 1, pp 22-29 Revised: 2018-02-14 doi:10.18052/www.scipress.com/sfp.1.22 Accepted: 2018-02-15 2018 SciPress Ltd., Switzerland Online:
More informationEffects of Hom Mali Brown Rice Flour Extract on Aspergillus niger Growth
Kasetsart J. (Nat. Sci.) 44 : 100-106 (2010) Effects of Hom Mali Brown Rice Flour Extract on Aspergillus niger Growth Porndarun Junlakun 1, Vichai Haruthaithanasan 2, Penkwan Chompreeda 1, 2 * and Walairut
More informationResearch & Reviews: Journal of Microbiology and Biotechnology
Research & Reviews: Journal of Microbiology and Biotechnology e-issn:2320-3528 Effect of Organic Acids on Bacterial Cellulose Produced by Acetobacter xylinum Hongmei Lu*, Qinghui Jia, Li Chen, and Liping
More informationCHAPTER 4 EXPERIMENTAL INVESTIGATION
51 CHAPTER 4 EXPERIMENTAL INVESTIGATION 52 MATERIALS CHEMICALS Potato Dextrose Agar (PDA), skim milk powder, nutrient agar medium, Sabouraud broth, Luria broth, Czapeck Dox broth were purchased from Himedia
More informationOPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS NIGER (BTM-26) IN SOLID STATE FERMENTATION
Pak. J. Bot., 46(3): 1071-1078, 2014. OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS NIGER (BTM-26) IN SOLID STATE FERMENTATION ROHEENA ABDULLAH 1, NAEEMA SHAHEEN
More informationScholars Research Library. Amylase production by fungi isolated from Cassava processing site
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 14, 4 (4):3-3 (http://scholarsresearchlibrary.com/archive.html) Amylase production by fungi isolated
More informationMalaysian Journal of Microbiology
Malaysian Journal of Microbiology, Vol 12(3) 2016, pp. 199-204 http://dx.doi.org/10.21161/mjm.71215 Malaysian Journal of Microbiology Published by Malaysian Society for Microbiology (In since 2011) Citric
More informationScientific Journal Impact Factor: (ISRA), Impact Factor: 2.114
[Meenakshi, 3(9): September, 214] ISSN: 2277-9655 IJESRT INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY Optimization of Slight Halotolerant α-amylase Producing Brevibacillus Borstelensis
More informationProduction of Acid Protease by Aspergillus niger Using Solid State Fermentation
Pakistan J. Zool., vol. (), pp. -, 9. Production of Acid Protease by Aspergillus niger Using Solid State Fermentation Hamid Mukhtar* and Ikram-Ul-Haq Institute of Industrial Biotechnology, GC University,
More informationALPHA-AMYLASE ASSAY PROCEDURE (CERALPHA METHOD) FOR THE MEASUREMENT OF PLANT AND MICROBIAL ALPHA-AMYLASES (100/200 Assays per Kit)
www.megazyme.com ALPHA-AMYLASE ASSAY PROCEDURE (CERALPHA METHOD) K-CERA 02/17 FOR THE MEASUREMENT OF PLANT AND MICROBIAL ALPHA-AMYLASES (100/200 Assays per Kit) AOAC Method 2002.01 AACC Method 22-02.01
More informationInternational Journal of Pure and Applied Sciences and Technology
Int. J. Pure Appl. Sci. Technol., 5(1) (2011), pp. 16-24 International Journal of Pure and Applied Sciences and Technology ISSN 2229-6107 Available online at www.ijopaasat.in Research Paper Isolation and
More informationProduction of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3)
Production of -Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS) Amornrat Chaikritsadakarn,*, Poonsuk Prasertsan, and Piyarat Boonsawang
More informationIJREAT International Journal of Research in Engineering & Advanced Technology, Volume 1, Issue 2, April-May, 2013 ISSN:
A Comparative Overview of Ethanol Production from Cereal Grains and Potato by Enzymatic Treatment Soumitra Banerjee 1, Debalina Kundu 2 Dept of Food Technology, Techno India Saltlake, Kolkata 700091 Abstract
More informationPelagia Research Library. Purification and comparison properties of crude enzyme with purified α-amylase from Bacillus licheniformis ATCC 6346
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2011, 1 (3):58-69 ISSN: 2248 9215 Purification and comparison properties of crude enzyme with purified α-amylase
More informationEvaluation of - amylase enzyme from Bacillus sp. isolated from various soil samples
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 783-789 http://www.ijcmas.com Original Research Article Evaluation of - amylase enzyme from Bacillus sp. isolated from various soil samples M.P.Prasad 1*,
More informationProduction of amylase from fruit peel using Aspergillus Niger by Solid State Fermentation
Available online at Scholars Research Library Der Pharmacia Lettre, 2014, 6 (2):173-177 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 Production of amylase from fruit
More informationBioprospecting of Neem for Antimicrobial Activity against Soil Microbes
ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu
More informationAMYLOGLUCOSIDASE from ASPERGILLUS NIGER, var.
AMYLOGLUCOSIDASE from ASPERGILLUS NIGER, var. SYNONYMS INS No. 1100 Prepared at the 59 th JECFA (2002) and published in FNP 52 Add 10 (2002), superseding tentative specifications prepared at the 55 th
More information5 Optimisation of Process Parameters of L- asparaginase production by isolate SI091
Optimisation of Process Parameters of L-asparaginase production by isolate SI91 69 5 Optimisation of Process Parameters of L- asparaginase production by isolate SI91 5.1 Introduction Success of bioprocess
More informationEnhanced Production and Characterization of Commercially Important Thermostable Amylolytic Enzyme
(An ISO 3297: 27 Certified Organization) Vol. 3, Issue 7, July 214 Enhanced Production and Characterization of Commercially Important Thermostable Amylolytic Enzyme Sarita Sharma 1, Rachana Bhatt 2 1 Master
More informationProduction and Preliminary Characterization of Alkaline Protease from Aspergillus flavus and Aspergillus terreus
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2010, 7(2), 479-482 Production and Preliminary Characterization of Alkaline Protease from Aspergillus flavus and Aspergillus terreus
More informationSurvival of Aerobic and Anaerobic Bacteria in
APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,
More informationStudy on The Inhibition Effect of Nisin
The Journal of American Science, 1(2), 2005, Li, et al, Study on The Inhibition Effect of Study on The Inhibition Effect of Tiejing Li 1, Jin Tao 2, Fu Hong 1 1. Northeast Agricultural University, Harbin,
More informationOptimization of various Nitrogen sources for the production of -Amylase using Brevibacillus borstelensis R1 by Submerged fermentation
ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 791-800 http://www.ijcmas.com Original Research Article Optimization of various Nitrogen sources for the production of -Amylase using Brevibacillus borstelensis
More informationOptimization of Lipase Production Medium for a Bacterial Isolate
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.5, No.6, pp 2837-2843, Oct-Dec 2013 Optimization of Lipase Production Medium for a Bacterial Isolate Sonia Dhiman 1 and
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(7): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(7):13-17 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Impact of Imidacloprid Intoxication on Amylase and
More informationOptimization of Operating Variables in the Fermentation of Citric acid using Response Surface Methodology
2011 2nd International Conference on Chemical Engineering and Applications IPCBEE vol. 23 (2011) (2011) IACSIT Press, Singapore Optimization of Operating Variables in the Fermentation of Citric acid using
More informationProduction and Characterization of Bacillus firmus Pectinase
Production and Characterization of Bacillus firmus Pectinase Anna Roosdiana, 1* Sasangka Prasetyawan, 1 Chanif Mahdi, 1 and Sutrisno 1 1 Laboratory of Biochemistry, Department of Chemistry, Faculty of
More informationEffect of nitrogen source on the growth and lipid production of microalgae
Effect of nitrogen source on the growth and lipid production of microalgae H. Varsha rani 1*, K. T.Vijaya Kumar and V. Eswarappa 1 Department of Agricultural Microbiology, University of Agricultural Sciences,
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationSUMMARY AND CONCLUSION
SUMMARY AND CONCLUSION A potential lipase producing marine fungus was selected among 14 lipase producers isolated from seawater and sediments of South Indian coastal environments which was identified as
More informationNEUTRAL PROTEASE PRODUCTION BY RHIZOPUS OLIGOSPORUS NCIM 1215 UNDER SOLID STATE FERMENTATION USING MIXED SUBSTRATES OF AGRO INDUSTRIAL RESIDUES
Int. J. Chem. Sci.: 11(1), 13, 291-35 ISSN 972-768X www.sadgurupublications.com NEUTRAL PROTEASE PRODUCTION BY RHIZOPUS OLIGOSPORUS NCIM 1215 UNDER SOLID STATE FERMENTATION USING MIXED SUBSTRATES OF AGRO
More informationResearch Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 09758585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) & Trigonella foenum graecum (Methi) against some selected
More informationAMYLASE FROM MODERATE HALOPHILES ISOLATED FROM WILD ASS EXCRETA Mrugesh Khunt 1*, Neepa Pandhi 2 and Archana Rana 2
Page586 IJPBS Volume Issue 4 OCT-DEC 2 586-592 AMYLASE FROM MODERATE HALOPHILES ISOLATED FROM WILD ASS EXCRETA Mrugesh Khunt *, Neepa Pandhi 2 and Archana Rana 2 Department of Plant Pathology, NMCA, Navsari
More informationTannase Production By Aspergillus niger
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net Vol. 4, No. 2, pp 192-198, April 2007 Tannase Production By Aspergillus niger N. LOKESWARI* and K. JAYA RAJU Center for Biotechnology,
More informationFactors affecting yeast growth and protein yield production from orange, plantain and banana wastes processing residues using Candida sp.
African Journal of Biotechnology Vol. 7 (3), pp. 9-95, 5 February, Available online at http://www.academicjournals.org/ajb ISSN 1 5315 Academic Journals Full Length Research Paper Factors affecting yeast
More informationInternational Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN
International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil
More information