Effect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste
|
|
- Scot Miles
- 5 years ago
- Views:
Transcription
1 Biotechnological Communication Biosci. Biotech. Res. Comm. 8(1): (2015) Effect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste Rupali R. Deshmukh and N. N. Vidhale* Department of Microbiology, Shri Shivaji Science College, Amravati , M.S., India ABSTRACT Agro industrial waste constitute valuable source for cultivation of microorganisms and production of enzyme. An attempt was made to study protease production at various ph by Fusarium oxysporum on agro industrial waste such as dal mill waste, oil mill waste, molasses, fruit waste and vegetable garbage. The maximum protease production (20.2 μg /ml) was obtained at ph 6 and 6.5 when dal mill waste was the substrate after 6 th day of incubation. Similarly with oil mill waste protease production was (19.6 μg /ml and 20.6 μg /ml) after 6 th and 7 th day of incubation respectively when ph was 5.5 and 6. However with vegetable garbage, maximum protease was (21.8 μg /ml) at ph 5 after 120 hrs of incubation. Fruit waste showed production of enzyme maximum (12.3 μg /ml) at ph 5 and 6.5 after 96 hrs of incubation KEY WORDS: AGRO INDUSTRIAL WASTE, FUSARIUM OXYSPORUM, PROTEASE. INTRODUCTION Enzymes are among the most important products obtained for human needs through microbial Sources. Protease enzyme constitutes two thirds of total enzymes used in various industries and this dominance in the industrial market need to increase production (Gupta et al., 2002).For production of enzyme for industrial use, isolation and characterization of strains using cheap carbon and nitrogen source is a continuous process (Parekh et al., 2002). This enzyme occurs widely in plants and animals, but commercially proteases are produced exclusively from microorganisms. Molds of the genera Aspergillus, Penicillium and Rhizopus are especially used for producing proteases (Sandhya et al., 2005 and Negi and Banerjee, 2010). MATERIAL AND METHODS SUBSTRATE PREPARATION Agro industrial waste such as dal mill waste, oil mill waste, Molasses, fruit waste and vegetable garbage were collected and powdered to size about 2 mm in homogenizer and then sieved through mesh screens to ARTICLE INFORMATION: *Corresponding Author: vidhalenana@gmail.com Received 20 th May, 2015 Accepted after revision 28 th June, 2015 BBRC Print ISSN: Online ISSN: NAAS Journal Score : 3.48 A Society of Science and Nature Publication, All rights reserved. Online Contents Available at: http// 78
2 obtain a particle having diameter between 0.42 to 0.85 mm. Each of such substrate was supplemented with 0.83 gm K 2 HPO 4 and 0.16gm MgSO 4, 1.5% agar-agar and 10 ml distilled water and autoclaved for 15 min. at 15 lb/ inch 2. Initial utilization of these substrates for production of protease by Fusarium oxysporum strain was studied under Solid state fermentation, incubated for 7 days at room temperature (25 to 30 0 C). Initially 20 g of 50% moistened substrate was sterilized and thoroughly mixed with 1ml spore suspension of 7 day old culture of Fusarium oxysporum. This substrate along with spore suspension was poured in sterilized Petri dish and allowed to incubate at room temperature for 7 days. ENZYME ESTIMATION After every 24 hrs of interval 1gm fermented substrate was harvested from petri plate and transferred to test tube containing 5ml phosphate buffer. The contents were homogenized and centrifuged at 2000 rpm for 30 min to remove all particulate matter. Protease activity was assayed as suggested by Keay and Wrildi (1970). To 1 ml of culture filtrate, 1ml of 2% casein solution was added and the mixture was incubated at 37 0 C for 10 min. The reaction was terminated by adding 2ml 0.4 M TCA (Trichloro acetic acid), again incubated at 37 0 C for 20 min. and filtered through Whatman filter paper no1. One ml of the filterate was added to 5 ml of sodium carbonate (0.4M) and 1ml Folin- Ciocalteus s reagent and incubated at 37 0 C for 30 min. The Absorbance was measured at 660 nm. in Spectrophotometer. EFFECT OF PH ON PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM All the five substrates were supplied with ph such as 5, 5.5, 6, 6.5 and 7 and subjected to SSF in Petri plates. Plates were incubated for 7 days. Enzyme activity was determined from 24 hrs to 7 days of incubation. Table 4: Effect of ph on production of protease by Fusarium oxysporum in solid state fermentation using agro industrial waste as substrate. Type of waste ph Production of protease (μg/ml )at different Incubation period Mean ± SD 24 hrs. 48 hrs. 72 hrs. 96 hrs. 120 hrs. 144 hrs. Value Dal mill waste Oil mill waste Molasses Fruit waste Vegetable garbage ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±7.46 BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS EFFECT OF PH ON THE PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM USING AGROINDUSTRIAL WASTE 79
3 RESULTS AND DISCUSSION All the five substrates were supplemented with different ph such as 5, 5.5, 6, 6.5, and 7 inoculated with Fusarium oxysporum and incubated for 7 days at room temperature under solid state fermentation. The production of protease was studied and mentioned in Table 4 and Fig.12, 13, 14, 15 and 16. From the result it has been found that the maximum protease production (20.2 μg /ml) was obtained at ph 6 and 6.5 when dal mill waste was the substrate after 6 th day of incubation. Similarly when oil mill waste was the substrate maximum protease production (19.6 μg /ml and 20.6 μg /ml) was recorded after 6 th and 7 th day of incubation respectively when ph was 5.5 and 6. However maximum protease production was recorded after 4 th and 5 th day of incubation at all ph in case of molasses. As far as vegetable garbage as a substrate is concerned again all the five types of ph gave maximum production of protease on 6 th day of incubation at room temperature, however maximum protease was seen (21.8 μg /ml) at ph 5 after 120 hrs of incubation. On the contrary fruit waste substrate responded comparatively lesser as it showed production of enzyme maximum (12.3 μg /ml) at ph 5 and 6.5 after 96 hrs of incubation when compared with other substrates. In general all the agro industrial waste when supplemented with ph 5.0, 5.5, 6.0 and 6.5 showed increase in protease production as compared to initial enzyme production without adjustment of phs. Studies dealing with the effect of initial ph on protease production by other Sreptomyces strains as S. rimosus and Streptomyces sp. 594 in SSF conditions indicated ph values between 6.0 and 7.0 for optimal protease activity (Yang et al., 1999). Ali and Vidhale, (2013) observed maximum protease production with initial ph of substrate 7.0 where as Negi and Banerjee, (2010) achieved optimum protease yield at ph 5.5.Production of protease using Rhizopus microsporus var oligosporus was studies by Sarao et al., (2009) showed maximum enzyme activity at initial ph 5.5. Similarly Radha et al., (2012) showed production of acid protease at ph 5.0.Optimum ph for protease production by Fusarium oxysporum was recorded at 7.0 in the study of Ali and Vidhale, (2013).The optimum ph of protease enzyme production by B. Subtilis and A.niger was recorded ph7 and 8 respectively by Oyeleke et al., (2012). FIGURE 12: Effect of ph on production of protease by Fusarium oxysporum in SSF using dal mill waste. 80 EFFECT OF PH ON THE PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM USING AGROINDUSTRIAL WASTE BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
4 FIGURE 13: Effect of ph on production of protease by Fusarium oxysporum in SSF using oil mill waste. FIGURE 14: Effect of ph on production of protease by Fusarium oxysporum in SSF using molasses. BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS EFFECT OF PH ON THE PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM USING AGROINDUSTRIAL WASTE 81
5 FIGURE 15: Effect of ph on production of protease by Fusarium oxysporum in SSF using fruit waste. FIGURE 16: Effect of ph on production of protease by Fusarium oxysporum in SSF using vegetable garbage. REFERENCES Gupta R., Beg Q. K. and Lorenz P. (2002), Bacterial Alkaline Protease: Molecular Approaches and Industrial Applications, Appl. Microbiol. Biotechnol. 59: Keay L. and Wrildi B. S. (1970), Biotechnol and Bioengg 72:719. Negi S. and Banerjee R. (2010). Optimization of Culture Parameters to Enhance Production of Amylase and Protease From Aspergillus awamori in a Single Fermentation. African Journal of Biochemistry Research 4 (3): Oyeleke S. B., Egwim E. C., Oyewole O. A., John E. E. (2012). Production of Cellulase and Protease from Microorganisms Isolated from Gut of Archachatina marginata (Giant African Snail) Science and Technology 2(1): Parekh S., Vinei V. A., Stroobel R. J. (2002). Alkaline Protease Production by Batch Culture of Bacillus sp. Appl Microbial Biotechnol 54:287:301. Radha S., Sridevi A., Himakiran Babu R., Nithya V. J., Prasad N. B. L. and Narasimha G. (2012). Medium Optimization for Acid protease production from Aspergillus sps under Solid 82 EFFECT OF PH ON THE PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM USING AGROINDUSTRIAL WASTE BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS
6 State Fermentation and Mathematical Modelling of Protease Activity. J. Microbiol. Biotech. Res. 2 (1): Sarao L., Arora M., Sehgal V., Bhatia S., (2009). Production of Protease by Submerged Fermentation Using Rhizopus Microsporus Var Oligospous. The Internet Journal of Microbiology Vol. 9 (1). Sandhya C., Sumantha A., Szakacs G. and Pandey A. (2005). Comparative evaluation of neutral protease production by Aspergillus oryzea in submerged and solid-state Fermentation. Biochemical process. 40: Ali S. S. and Vidhale N. N. (2013): Protease Production by Fusarium oxysporum in Solid- State Fermentation Using Rice Bran. American journal of microbiological research 1(3): Yang S. and Wang J. (1999). Protease and Amylase Production of Streptomyces rimosus in Submerged and Solid State Cultivation. Bot Bull Acad Sin 40: BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS EFFECT OF PH ON THE PRODUCTION OF PROTEASE BY FUSARIUM OXYSPORUM USING AGROINDUSTRIAL WASTE 83
Effect of incubation temperature on production of amylase in various agroindustrial wastes by alkaliphilic actinobacteria
Bioscience Discovery, 8(3): 521-527, July - 2017 RUT Printer and Publisher Print & Online, Open Access, Research Journal Available on http://jbsd.in ISSN: 2229-3469 (Print); ISSN: 2231-024X (Online) Research
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 211, 1 (3):124-129 ISSN: 2248 9215 Production of Alkaline Protease by Bacillus subtilis (MTCC7312) using Submerged
More informationScreening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal
Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Article Summary (In short - What is your article about Just 2 or 3 lines) Category: Bacillus sp produce
More informationEXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN
BIOLOGIA 2001, 47 (1&2), PP 47 52 ISSN 0006 3096 EXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN *HAMAD ASHRAF, IKRAM UL HAQ, AND JAVED IQBAL Biotechnology Research Laboratory, Department
More informationINTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY
[Ravish, 2(2): Feb., 2013] ISSN: 2277-9655 IJESRT INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY Isolation And Characterization Of Proteolytic Bacteria And Its Protease Himani Ravish
More informationNEUTRAL PROTEASE PRODUCTION BY RHIZOPUS OLIGOSPORUS NCIM 1215 UNDER SOLID STATE FERMENTATION USING MIXED SUBSTRATES OF AGRO INDUSTRIAL RESIDUES
Int. J. Chem. Sci.: 11(1), 13, 291-35 ISSN 972-768X www.sadgurupublications.com NEUTRAL PROTEASE PRODUCTION BY RHIZOPUS OLIGOSPORUS NCIM 1215 UNDER SOLID STATE FERMENTATION USING MIXED SUBSTRATES OF AGRO
More informationScholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2012, 2 (4):612-618 (http://scholarsresearchlibrary.com/archive.html) Purification and characterization
More informationEffect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S.
Available online at www.sciencedirect.com APCBEE Procedia 00 (2012) 000 000 Conference title Effect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S. Chuenjit
More informationJOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.625, ISSN: , Volume 2, Issue 11, December 2014
COMPARISON OF ACTIVITY OF PECTINASE USING AGRICULTURAL WASTE SUBSTRATES A STUDY M.P.KUSUMA* DR M.V.V.CHANDANA LAKSHMI** *Assistant Professor, RBVRR College of Pharmacy, Osmania University, India **Associate
More informationAspergillus foetidus BY AQUEOUS TWO PHASE
33 CHAPTER 3 PARTIAL PURIFICATION OF TANNASE FROM Aspergillus foetidus BY AQUEOUS TWO PHASE EXTRACTION AND ITS CHARACTERIZATION 3.1 INTRODUCTION Partial purification of proteins in general and tannase
More informationExploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste
Int. J. of Life Sciences, 2016, Vol. 4 (3): 394-400 ISSN: 2320-7817 eissn: 2320-964X RESEARCH ARTICLE Exploration of Alkaliphilic Actinomycetes for Amylase Production from Agroindustrial Waste Vidhale
More informationProduction and Preliminary Characterization of Alkaline Protease from Aspergillus flavus and Aspergillus terreus
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2010, 7(2), 479-482 Production and Preliminary Characterization of Alkaline Protease from Aspergillus flavus and Aspergillus terreus
More informationJournal of Chemical and Pharmaceutical Research, 2012, 4(8): Research Article
Available online wwwjocprcom Journal of Chemical and Pharmaceutical Research, 212, 4(8):388-3812 Research Article ISSN : 975-7384 CODEN(USA) : JCPRC5 Isolation and partial purification of Protease from
More informationAmylase Production from Potato and Banana Peel Waste
Int.J.Curr.Microbiol.App.Sci (213) 2(11): 41-414 ISSN: 2319-776 Volume 2 Number 11 (213) pp. 41-414 http://www.ijcmas.com Original Research Article Amylase Production from Potato and Banana Peel Waste
More informationInducers for the enhanced production of lipase by Streptomyces isolated from mangrove ecosystem
Int.J.Curr.Microbiol.App.Sci (24) 3() 37-376 ISSN: 239-776 Volume 3 Number (24) pp. 37-376 http://www.ijcmas.com Original Research Article s for the enhanced production of lipase by Streptomyces isolated
More informationOPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE
Int. J. Chem. Sci.: 8(2), 2010, 909-913 OPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE T. SATHISH a and N. Y. S. MURTHY * Department of Biotechnology, Malla Reddy Engineering College, HYDERABAD (A.P.)
More informationMedia Optimization Studies for Enhanced Production of Serratiopeptidase
Media Optimization Studies for Enhanced Production of Serratiopeptidase from Bacillus Licheniformis (NCIM ) Manasi J. Wagdarikar*, Anagha M. Joshi, Amir A. Shaikh SCES s Indira College of Pharmacy, Tathawade,
More informationProduction of Thermostable and Ca +2 Independent α-amylases from Halphilic Bacteria
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 12, Number 2 (2016) pp. 153-159 Research India Publications http://www.ripublication.com Production of Thermostable and Ca
More informationProduction and Optimization of Protease from Aspergillus niger and Bacillus subtilis using Response Surface Methodology
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 2, Issue 7 (Nov. Dec. 2016), PP 01-07 Production and Optimization of Protease from Aspergillus niger and Bacillus subtilis
More informationOPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL DESIGN
http://www.rasayanjournal.com Vol.4, No.1 (2011), 159-164 ISSN: 0974-1496 CODEN: RJCABP OPTIMIZATION OF PROTEASE PRODUCTION FROM HUSK OF VIGNA MUNGO BY BACILLUS SUBTILIS NCIM 2724 USING STATISTICAL EXPERIMENTAL
More informationOptimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation
African Journal of Biochemistry Research Vol. 4(3), pp. 73-8, March 21 Available online at http://www.academicjournals.org/ajbr ISSN 1996-778 21 Academic Journals Full Length Research Paper Optimization
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationResearch & Reviews: Journal of Microbiology and Biotechnology
Research & Reviews: Journal of Microbiology and Biotechnology e-issn:2320-3528 Effect of Organic Acids on Bacterial Cellulose Produced by Acetobacter xylinum Hongmei Lu*, Qinghui Jia, Li Chen, and Liping
More informationStudy on moisture content of substrate (Sesamum oil cake and Rice chaff) on the yield of Citric acid
Int.J.Curr.Microbiol.App.Sci (14) 3(5): 138-144 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-776 Volume 3 Number 5 (14) pp. 138-144 http://www.ijcmas.com Original Research
More informationProduction of lipase from Aspergillus oryzae (T4) isolate by solidstate. Aspergillus oryza (T4)
Production of lipase from Aspergillus oryzae (T4) isolate by solidstate fermentation Aspergillus oryza (T4) Thikra A. Mustafa Shatha S.AL-Azawi* Khulood W.AL-Samarraei** Biology Department/ Collage of
More informationEFFECT OF ENDOSULFAN AND CHLORPYRIFOS ON PROTEASE ACTIVITY IN THE CULTIVATED SOIL Sumit Kumar*
Research Article EFFECT OF ENDOSULFAN AND CHLORPYRIFOS ON PROTEASE ACTIVITY IN THE CULTIVATED SOIL Sumit Kumar* Address for Correspondence * Biotechnology Engineering Department, V.V.P. Engineering College,
More informationPreliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp.
frican Journal of iotechnology Vol. 6 (1), pp. 28-33, 4 January 27 vailable online at http://www.academicjournals.org/j ISSN 1684 5315 27 cademic Journals Full Length Research Paper Preliminary studies
More informationScreening of Aspergillus flavus and Aspergillus fumigatus strains for extracellular protease enzyme production
Journal of Microbiology and Antimicrobials Vol. 2(7), pp. 83-87, October 2010 Available online http://www.academicjournals.org/jma ISSN 2141-2308 2010 Academic Journals Full Length Research Paper Screening
More informationCHAPTER 3 OPTIMIZATION OF PROCESS PARAMETERS FOR PROTEASE PRODUCTION IN SOLID STATE FERMENTATION
76 CHAPTER 3 OPTIMIZATION OF PROCESS PARAMETERS FOR PROTEASE PRODUCTION IN SOLID STATE FERMENTATION 3.1 INTRODUCTION Solid State Fermentation (SSF) is defined as the cultivation of microorganisms on moist
More informationProduction of amylase from fruit peel using Aspergillus Niger by Solid State Fermentation
Available online at Scholars Research Library Der Pharmacia Lettre, 2014, 6 (2):173-177 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 Production of amylase from fruit
More informationASSAY OF USING BETA-GLUCAZYME TABLETS
ASSAY OF endo-β-glucanases USING BETA-GLUCAZYME TABLETS T-BGZ 12/12 Note: Changed assay format for malt β-glucanase Megazyme International Ireland 2012 SUBSTRATE: The substrate employed is Azurine-crosslinked
More informationFormulation of alternative culture media for bacterial and fungal growth
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (1):431-436 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationScreening, Isolation and Characterization of Amylase Producing Bacteria and optimization for Production of Amylase
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 4, Issue 2 (Mar. - Apr 2018), PP 50-56 www.iosrjournals.org Screening, Isolation and Characterization of Amylase Producing
More informationLactic acid production from rice straw using plant-originated Lactobacillus rhamnosus PN04
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(5):590-594 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Lactic acid production from rice straw using plant-originated
More informationHigher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).
4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine
More informationProduction and Optimization of Citric Acid by Aspergillus Niger Isolated from Rotted Onion Bulb
Production and Optimization of Citric Acid by Aspergillus Niger Isolated from Rotted Onion Bulb Cholke Snehal S 1, Mahale Suvarna D 2, Darekar Kalyani P 3, Daithankar Rohini N 4 1,2,3,4New Arts, Commerce
More information--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009.
Translated English of Chinese Standard: GB5009.259-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD GB OF THE PEOPLE S REPUBLIC OF CHINA National food safety standard Determination
More informationEFFECT OF NITROGEN SOURCE FOR THE BIOSYNTHESIS OF XYLANASE FROM ASPERGILLUS TAMARII
Lingappa Journal of Drug Delivery & Therapeutics. ; (6):8-8 Available online on.. at http://jddtonline.info Journal of Drug Delivery and Therapeutics Open access to Pharmaceutical and Medical research,
More informationRecipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones)
Protocol: 300 ml Yeast culture preparation Equipment and Reagents needed: Autoclaved toothpicks Shaker Incubator set at 30 C Incubator set at 30 C 60 mm 2 sterile petri dishes Autoclaved glass test tubes
More informationAMYLAZYME RED TABLETS
www.megazyme.com AMYLAZYME RED TABLETS For the measurement of cereal and microbial α-amylases T-AMZRD 08/16 Megazyme International Ireland 2016 INTRODUCTION: The level of α-amylase in wheat and barley
More informationProcess parameters influencing tannase production by Aspergillus niger using mangrove (Rhizophora apiculata) bark in solid substrate fermentation
African Journal of Biotechnology Vol. 10(61), pp. 13147-13154, 10 October, 2011 Available online at http://www.academicjournals.org/ajb DOI: 10.5897/AJB10.1437 ISSN 1684 5315 2011 Academic Journals Full
More informationCELLULASE from PENICILLIUM FUNICULOSUM
CELLULASE from PENICILLIUM FUNICULOSUM Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in FNP
More informationPuducherry. Antimicrobial activity, Crude drug extraction, Zone of Inhibition, Culture Media, RVSPHF567.
ANTI-MICROBIAL ACTIVITY OF THE CRUDE DRUGS AND THE POLYHERBAL FORMULATION (RVSPHF567) BY STANDARDIZED CUP AND PLATE METHOD C.S. Kandasamy 1,2*, Suman Nath 2, P. Arulraj 1,2, V. Gopal 3, P. Muthusamy 4,
More informationLaboratorios CONDA, S.A. Distributed by Separations
Culture Media as on Pharmacopoeia 7.3, Harmonized Method for Microbiological Examination of non sterile products -FORMULATIONS Buffered sodium chloride-peptone solution ph 7.0 Cat. Nº 1401 Potassium dihydrogen
More informationCHAPTER 4 EXPERIMENTAL INVESTIGATION
51 CHAPTER 4 EXPERIMENTAL INVESTIGATION 52 MATERIALS CHEMICALS Potato Dextrose Agar (PDA), skim milk powder, nutrient agar medium, Sabouraud broth, Luria broth, Czapeck Dox broth were purchased from Himedia
More informationBioprospecting of Neem for Antimicrobial Activity against Soil Microbes
ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu
More informationCellulase production by Aspergillus niger under solid state fermentation using agro industrial wastes
Research Article SOI: http://s-o-i.org/1.15/ijarm-2016-3-2-12 Cellulase production by Aspergillus niger under solid state fermentation using agro industrial wastes N. Mahalakshmi* and S. Jayalakshmi CAS
More informationAcid Protease Production by Fungi Used in Soy3bean Food
APPLIED MICROBIOLOGY, May 1974, p. 906-911 Copyright 1974 American Society for Microbiology Vol. 27, No. 5 Printed in U.S.A. Acid Protease Production by Fungi Used in Soy3bean Food Fermentation HWA L.
More informationMalaysian Journal of Microbiology
Malaysian Journal of Microbiology, Vol 12(3) 2016, pp. 199-204 http://dx.doi.org/10.21161/mjm.71215 Malaysian Journal of Microbiology Published by Malaysian Society for Microbiology (In since 2011) Citric
More informationIsolation and Screening of Citric Acid Producing Aspergillus Spp and Optimisation of Citric Acid Production by Aspergillus Niger S-6
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-issn: 2319-2402,p- ISSN: 2319-2399.Volume 9, Issue 3 Ver. I (Mar. 2015), PP 19-23 www.iosrjournals.org Isolation and
More informationOptimization of saccharification conditions of prebiotic extracted jackfruit seeds
Paper Code: fb005 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Optimization of saccharification conditions of prebiotic extracted jackfruit seeds Sininart Chongkhong *, Bancha
More informationProduction and optimization of cellulase from Fusarium oxysporum by submerged fermentation
Journal of Scientific & Industrial Research 454 Vol. 69, June 2010, pp. 454-459 J SCI IND RES VOL 69 JUNE 2010 Production and optimization of cellulase from Fusarium oxysporum by submerged fermentation
More informationPrinciples of Biotechnology INDUSTRIAL BIOTECHNOLOGY WEEKS 8+9
Principles of Biotechnology INDUSTRIAL BIOTECHNOLOGY WEEKS 8+9 Industrial Microbiology Industrial Microorganisms and Product formation involved: 1- Use microorganisms to produce valuable commercial product
More informationComparative Study of Citric Acid Production from Annona reticulata and Its Peel with Effect of Alcohol as a Stimulant
International Journal of Advanced Biotechnology and Research(IJBR) ISSN 0976-2612, Online ISSN 2278 599X, Vol5, Issue3, 2014, pp346-352 http://www.bipublication.com Comparative Study of Citric Acid Production
More informationHeterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as a Carbon Substrate
2011 International Conference on Food Engineering and Biotechnology IPCBEE vol.9 (2011) (2011)IACSIT Press, Singapoore Heterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as
More informationExtraction and Characterization of Protease From Senesced Leaves of Papaya (Carica Papaya) and It s Application
International Journal of Genetic Engineering and Biotechnology. ISSN 0974 3073 Volume 5, Number 1 (2014), pp. 29-34 International Research Publication House http://www.irphouse.com Extraction and Characterization
More informationIsolation and Screening of Amylase Producing Fungi
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 783-788 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.604.098
More informationDegradation of chicken feathers by Leuconostoc sp. and Pseudomonas microphilus
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2012, 2 (2):358-362 Degradation of chicken feathers by Leuconostoc sp. and Pseudomonas microphilus ISSN: 2248
More informationStatistical screening of factors affecting glucoamylase production by a thermotolerant Rhizopus microsporus TISTR 3518 using Plackett-Burman design
African Journal of Biotechnology Vol. 9(43), pp. 7312-7316, 25 October, 2010 Available online at http://www.academicjournals.org/ajb DOI: 10.5897/AJB10.049 ISSN 1684 5315 2010 Academic Journals Full Length
More informationChapter 8. Optimization of growth. conditions favoring maximum. lipase production by selected. isolates
Chapter 8 Optimization of growth conditions favoring maximum lipase production by selected isolates Microbial lipases are mostly produced in submerged culture (Ito et al., 2001) but solid state fermentation
More informationScreening of Rice Straw Degrading Microorganisms and Their Cellulase Activities
Research 83 KKU Sci. J.37 (Supplement) 83-88 (2009) Screening of Rice Straw Degrading Microorganisms and Their Cellulase Activities Abstract Atcha Boonmee 1,2* Rice straw is one of the most abundant agricultural
More informationOptimization of Trichoderma reesei Medium for Increasing Xylanase Enzyme Production
Advances in Bioresearch Adv. Biores., Vol 7 (3) May 2016: 94-99 2015 Society of Education, India Print ISSN 0976-4585; Online ISSN 2277-1573 Journal s URL:http://www.soeagra.com/abr.html CODEN: ABRDC3
More informationInternational Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN
International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil
More informationProduction of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3)
Production of -Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS) Amornrat Chaikritsadakarn,*, Poonsuk Prasertsan, and Piyarat Boonsawang
More informationTrial Application of Bacterial Cellulose Membrane in Minimally Pre-Prepared Fresh Coconut
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 6 Issue 4 April 2017 PP. 32-37 Trial Application of Bacterial Cellulose Membrane in Minimally
More informationSCREENING AND PRODUCTION OF α-amylase FROM ASPERGILLUS NIGER USING ZERO VALUE MATERIAL FOR SOLID STATE FERMENTATION
Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 975-1491 Vol 5, Issue 1, 13 Research Article SCREENING AND PRODUCTION OF α-amylase FROM ASPERGILLUS NIGER USING ZERO
More informationMATERIAL AND METHODS
MATERIAL AND METHODS Material and Methods Glucose induced cataract was chosen as a model for the present study. A total of 210 fresh goat lenses were analyzed. Sample Collection: Goat eyeballs were obtained
More informationAZO-XYLAN (BIRCHWOOD)
ASSAY OF endo-1,4-ß-xylanase using AZO-XYLAN (BIRCHWOOD) S-AXBP S-AXBL 10/07 Megazyme International Ireland 2007 PRINCIPLE: This assay procedure is specific for endo-1,4-ß-d-xylanase activity. On incubation
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD OF
Translated English of Chinese Standard: GB4789.40-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.40-2016 National Food Safety Standard
More informationTannase Production By Aspergillus niger
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net Vol. 4, No. 2, pp 192-198, April 2007 Tannase Production By Aspergillus niger N. LOKESWARI* and K. JAYA RAJU Center for Biotechnology,
More informationEFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-110
BIOLOGIA 21, 47 (1&2), PP 59 65 ISSN 6 396 EFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-11 SIKANDER ALI 1, IKRAM-UL-HAQ 1 AND JAVED IQBAL 2 1 Biotechnology Research
More informationEnzymatic Assay of PROTEASE (EC )
Enzymatic Assay of PROTEASE PRINCIPLE: Hemoglobin + H 2 O Protease > Amino Acids CONDITIONS: T = 37 C, ph = 2.8, A 660nm, Light path = 1 cm METHOD: Colorimetric REAGENTS: A. 50 mm Potassium Phthalate Buffer,
More informationIsolation Of Tannase Producing Fungi And Optimization Of Culture Conditions For Tannase Production By Fungus tws-3
Original Paper ISSN: 2321-1520 Isolation Of Tannase Producing Fungi And Optimization Of Culture Conditions For Tannase Production By Fungus tws-3 Rakeshkumar R. Panchal 1, Hinal Dhaduk 2, Kiransinh N Rajput
More informationA novel bi-substrate fermentation (BSF) process was developed for the production of lipase from Aspergillus niger using
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com UTILISATION OF SOLID WASTES FROM TANNERY FOR THE PRODUCTION OF LIPASE FROM ASPERGILLUS NIGER BY MIXED SUBSTRATE
More informationEvaluation of agro-industrial wastes for conidia based inoculum production of bio-control agent: Trichoderma harzianum
Journal of Scientific & Industrial Research Vol. 63, October 2004, pp 807-812 Evaluation of agro-industrial wastes for conidia based inoculum production of bio-control agent: Trichoderma harzianum Lakshmi
More informationProduction of Cellulase from Aspergillus fumigatus Under Submerged and Solid State Fermentation Using Agricultural Waste
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Production of Cellulase from Aspergillus fumigatus Under and Solid State Using Agricultural Waste P. Shobana and N.
More informationAppendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water
Appendix A: Preparation of Media and Chemicals Preparation of Malt Extract Agar (MEA) Malt Extract Agar (MEA) weighing 13.44 g was dissolved in 400 ml of distilled water in an Erlenmeyer flask using a
More informationAMYLAZYME
www.megazyme.com AMYLAZYME ALPHA-AMYLASE ASSAY PROCEDURE T-AMZ 05/18 For the Measurement of Cereal and Microbial α-amylases AACC Method 22-05.01 Megazyme 2018 INTRODUCTION: The level of α-amylase in wheat
More informationBIODEGRADATION OF KERATIGENOUS WASTES BY DERMATOPHYTIC FUNGI WITH REFERENCE TO TRICOPHYTON RUBRUM
BIODEGRADATION OF KERATIGENOUS WASTES BY DERMATOPHYTIC FUNGI WITH REFERENCE TO TRICOPHYTON RUBRUM * R.A. Shah, S. Khan and R. Shaikh The Institute of Science, 15 Madame Cama Road, Mumbai 400 032, India
More informationSurvival of Aerobic and Anaerobic Bacteria in
APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,
More informationINFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and
INFLUENCE of PARTICLE SIZE on CITRIC ACID PRODUCTION by Aspergillus niger USING RICE CHAFF and SESAMUM OIL CAKE as SUBSTRATES S M Gopinath, Department of Biotechnology, Acharya Institute of Technology,
More informationCAMELLIA SINENSIS AS A NATURAL MEAT PRESERVATIVE IN ASSOCIATION WITH ITS TOTAL POLYPHENOL CONTENT
CAMELLIA SINENSIS AS A NATURAL MEAT PRESERVATIVE IN ASSOCIATION WITH ITS TOTAL POLYPHENOL CONTENT 1 Karandikar Pranali, 2 Bhatte Sukhada, 3 Ghayal Madhura 1 Student, 2 Lecturers, 3 Associate Professor
More informationGlucose Oxidase Pellets
BIOTECHNOLOGY AND BIOENGINEERING VOL. XIX (1977) Glucose Oxidase Pellets INTRODUCTION Considerable world-wide interest has arisen in the use of immobilized enzymes as catalysts in industrial process and
More informationASSAY OF using AZO-FRUCTAN S-AZFR5 11/17
www.megazyme.com ASSAY OF endo-fructanase using AZO-FRUCTAN S-AZFR5 11/17 Megazyme 2017 PRINCIPLE: The substrate is the high molecular weight fraction of chicory fructan (DP ~ 20-60) dyed with an azo-dye
More informationFactors affecting yeast growth and protein yield production from orange, plantain and banana wastes processing residues using Candida sp.
African Journal of Biotechnology Vol. 7 (3), pp. 9-95, 5 February, Available online at http://www.academicjournals.org/ajb ISSN 1 5315 Academic Journals Full Length Research Paper Factors affecting yeast
More informationScreening of Nutritional Parameters for the Production of Protease from Aspergillus Oryzae
ISSN: 0973-4945; CODEN ECJHAO E-Journal of Chemistry http://www.e-journals.net Vol. 4, No. 2, pp 208-215, April 2007 Screening of Nutritional Parameters for the Production of Protease from Aspergillus
More informationPROTAZYME AK TABLETS
www.megazyme.com ASSAY OF endo-protease using PROTAZYME AK TABLETS T-PRAK 05/16 Megazyme International Ireland 2016 SUBSTRATE: The substrate employed is Azurine-crosslinked casein (AZCL-casein). This substrate
More informationParametric Optimization for Extracellular Tannase Production in Submerged Fermentation by Isolated Aspergillus Species
ISSN: 2319-7706 Volume 4 Number 4 (2015) pp. 232-239 http://www.ijcmas.com Original Research Article Parametric Optimization for Extracellular Tannase Production in Submerged Fermentation by Isolated Aspergillus
More informationAPPENDIX Reagents. Appendix. Alsever s solution Citric acid 0.55g Sodium citrate 8.0g D-glucose 20.5g Sodium Chloride 4.2g
APPENDIX Reagents Alsever s solution Citric acid 0.55g Sodium citrate 8.0g D-glucose 20.5g Sodium Chloride 4.2g 1000 ml Barritt s reagent A α-naphthol 5.0g ethanol 95.0 ml α-naphthol was dissolved in ethanol
More informationInduced biochemical changes in the CMV infected cucurbit plants
Available online at www.scholarsresearchlibrary.com Annals of Biological Research, 2012, 3 (2):863-870 (http://scholarsresearchlibrary.com/archive.html) ISSN 0976-1233 CODEN (USA): ABRNBW Induced biochemical
More informationExtraction of Enzymes from Potato Peels Substrate using Bacillus subtilis
ISSN: 2319-7706 Volume 4 Number 8 (2015) pp. 451-458 http://www.ijcmas.com Original Research Article Extraction of Enzymes from Potato Peels Substrate using Bacillus subtilis Ashish Joshi 1 *, M.N. Dabhi
More informationJuthamat Ratha and Chaowaree Adthalungrong *
965 KKU Res. J. 2012; 17(6):965-971 http : //resjournal.kku.ac.th Selection of nutrient parameters for endoglucanase production from rice bran by Penicillium sp. using Plackett-Burman design Juthamat Ratha
More informationASSAY OF using CELLAZYME C TABLETS T-CCZ 01/17
www.megazyme.com ASSAY OF endo-cellulase using CELLAZYME C TABLETS T-CCZ 01/17 Megazyme 2017 SUBSTRATE: The substrate employed is azurine-crosslinked HE-cellulose (AZCL-Cellulose). This substrate is prepared
More informationSupplementary Information. Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food
Supplementary Information Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena and Debendra K. Sahoo* Biochemical
More informationIsolation and Screening of Starch Hydrolising Bacteria and its Effect of Different Physiological. Parameters on Amylase Enzyme Activity
, pp: 79-83 NOVEMBER-2015 Research Article (Open access) Isolation and Screening of Starch Hydrolising Bacteria and its Effect of Different Physiological Parameters on Amylase Enzyme Activity Prerana Min*,
More informationResearch Article ISSN Vol 2/Issue 4/Oct-Dec 2012 PRAGYA RATHORE*, PRATIK SHAH, HARSHPREET CHANDOK, SATYENDRA PATEL
THE PHOSPHATE SOLUBILIZING POTENTIAL OF CITROBACTER FREUNDII UNDER VARIOUS PHYSICOCHEMICAL CONDITIONS PRAGYA RATHORE*, PRATIK SHAH, HARSHPREET CHANDOK, SATYENDRA PATEL Sanghvi Institute of Management &
More informationPotential of Different Light Intensities on the Productivity of Spirulina maxima
Potential of Different Light Intensities on the Productivity of Spirulina maxima J. P. Pandey *, Amit Tiwari, Suchita Singh and Dileep Tiwari 1 Department of Biotechnology, Govt. T. R. S. College, Rewa
More informationEFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY
J. Gen. App!. Microbiol,, 21, 51-59 (1975) EFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY CLOSTRIDIUM ACETOBUTYLICUM BURT ENSLEY, JOHN J. McHUGH, AND LARRY L. BARTON Department
More informationIJREAT International Journal of Research in Engineering & Advanced Technology, Volume 1, Issue 2, April-May, 2013 ISSN:
A Comparative Overview of Ethanol Production from Cereal Grains and Potato by Enzymatic Treatment Soumitra Banerjee 1, Debalina Kundu 2 Dept of Food Technology, Techno India Saltlake, Kolkata 700091 Abstract
More informationContinental J. Microbiology 4: 1-7, 2010 ISSN: Wilolud Journals, 2010
Continental J. Microbiology : - 7, ISSN: - 6 Wilolud Journals, http://www.wiloludjournal.com AMYLOLYTIC PROPERTIES OF FUNGI ASSOCIATED WITH SPOILAGE IN BREAD Okoko, F.J and Ogbomo, O. Microbiology Department,
More information