Cellular Respiration an overview Section 9.1

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1 Cellular Respiration an overview Section 9.1

2 Where do organisms get their energy? Unit calories 1 calorie = amount of energy required to increase 1 gram of water by 1 degrees Celsius 1000 calories 1 Calorie Cells can use all sorts of food as energy

3 Examples 1 gram of sugar = 3811 calories (3.8 Calories) 1 gram of beef fat = 8893 calories (8.9 Calories) Carbohydrates and proteins approximately 4000 (4 Calories) calories per gram Fats- approximately 9000 calories (9 Calories) per gram

4 Cellular respiration overview It does not happen instantaneously!

5 Stages of cellular respiration Pyruvic acid enters in Krebs cycle Most energy generated in electron transport chain Cells can use variety of food sources Glucose most important Only a small amount of energy is liberated during glycolysis

aerobic Krebs cycle Electron transport chain Occur in mitochondria Glycolysis is

6 Role of oxygen Oxygen required at the end of the electron transport chain If cell needs energy it needs oxygen We need to breathe to respire Cellular respiration with oxygen is aerobic Krebs cycle Electron transport chain Occur in mitochondria Glycolysis is anaerobic Occurs in cell cytoplasm Can continue without oxygen fermentation Keeps glycolysis running

7 Photosynthesis vs Respiration Photosynthesis removes CO2 Respiration replaces CO2 Photosynthesis releases oxygen Respiration requires oxygen Virtually all living things respire, but only plants and some bacteria can photosynthesize

8 The process of cellular respiration Section 9.2

9 Glycolysis The first set of reactions Means sugar breaking Glucose is transformed through chemical steps Pyruvic acid is generated (3 carbons) As carbon bonds are broken energy is released

10 Overview of glycolysis Energy is required to break glucose Overall net energy gain in the reaction NAD+ accepts high energy electrons Process is very fast Thousands of ATP molecules produced in milliseconds Does not require oxygen

11 Krebs cycle Pyruvic acid is broken down to carbon dioxide by a series of energy forming reactions Also known as citric acid cycle Pyruvic acid passes through mitochondrial membrane into matrix 1 carbon atom from pyruvic acid becomes part of CO2 molecule Remaining carbon form acetic acid In the Krebs cycle, the acetic acid (acetycl CoA) combines with a 4-carbon compound Produces citric acid

12 Krebs cycle continued. Citric acid is progressively broken down to a 4 carbon molecule again Electrons are transported to NADH and FADH 2 At 5 points around the cycle Each cycle one molecule of ADP is converted to ATP 1 pyruvic acid = 1 ATP Carbon dioxide is released ATP can be used straight away NADH and FADH 2 feed the electron transport chain

13 Electron transport and ATP synthesis NADH generated from glycolysis enters the mitochondria and joins electron carriers produced by Krebs cycle These all feed into the electron transport chain In Eukaryotes located in inner membrane of mitochondrion In Prokaryotes located in cell membrane At the end of the chain an enzyme combines electrons to form water Oxygen is the final acceptor of electrons and removes waste H+ ions Without oxygen the chain can t function!

Electron transport and ATP synthesis cont The energy from the electrons is used to pump H+ ions across the membrane H+ ions build up in intermembrane space Becomes positively charged relative to the

14 Electron transport and ATP synthesis cont The energy from the electrons is used to pump H+ ions across the membrane H+ ions build up in intermembrane space Becomes positively charged relative to the matrix Matrix is negatively charged relative to membrane space The potential energy from this charge difference to generate ATP via chemiosmosis ATP generated from turbine like ATP synthase One pair of high energy electrons can generate 3 ATP molecules

15 How much ATP does cellular respiration generate? Together glycolysis, the krebs cycle and electron transport chain generate 36 molecules of ATP per glucose molecule Produces 18 times the amount possible by anaerobic respiration 36 ATP vs 2 ATP We don t just eat carbohydrates Lipids and proteins enter the Krebs cycle or glycolysis at one of many places Cells can generate ATP from many things 36 ATP is 36 % of total energy of glucose Remaining 64 % released as heat Explains why body temp is 37 C Why you feel warm after exercise

16 Fermentaion Section 9.3

17 Glycolysis Glycolysis can generate energy when oxygen isn t available BUT, in a few seconds all of a cell s NADP+ ions are full of electrons No oxygen means no electron transport chain Without NADP+ glycolysis stops Fermentation saves the day..

18 Fermentation Cells convert NADH to NAD+ - high energy electrons are passed back to pyruvic acid Glycolysis can continue happening Fermentation is anaerobic occurs in cell cytoplasm Two types Alcoholic fermentation Lactic acid fermentation

19 Alcoholic fermentation Yeasts and some other microorganisms use alcoholic fermentation to produce ethyl alcohol and carbon dioxide Used to make bread rise, used to make alcohol

20 Lactic acid fermentation Most organisms can perform lactic acid fermentation No carbon dioxide is given off Lactic acid from bacteria can be used to make cheese, yoghurt, sour cream Acid = sour taste Humans produce lactic acid Muscles are most adapted to do this, as they often need large supplies of ATP

21 Energy and exercise Humans have three sources of energy for sports ATP already in muscles Enough for a few seconds ATP made by lactic acid formation Enough ATP for 90 seconds ( meter sprint ) Large amounts of lactic acid builds up, an oxygen debt that must be repaid

Energy and exercise continued ATP made from cell respiration The only way to supply muscles with energy for a long amount of time Releases energy slowly why athletes pace themselves Glycogen stored

22 Energy and exercise continued ATP made from cell respiration The only way to supply muscles with energy for a long amount of time Releases energy slowly why athletes pace themselves Glycogen stored in muscles provides energy for 20 minutes or so After that other molecules are broken down Help people lose weight Allow animals to hibernate At the beginning of a race muscles use all forms of energy, but stored ATP and lactic acid fermentation can only supply energy for a limited time

9-1 Chemical Pathways

9-1 Chemical Pathways 2 of 39 Food serves as a source of raw materials for the cells in the body and as a source of energy. Animal Cells Animal Mitochondrion Plant Plant Cells 3 of 39 1 Both plant and animal cells carry out