By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA
|
|
- Nathaniel Merritt
- 5 years ago
- Views:
Transcription
1 By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA
2 PROBLEM Saccharin is consumed by many humans on a day to day basis. Overconsumption of saccharin may cause different types of cancer within the human body. However, what it does on microbial flora is unknown.
3 BACKGROUND ON SACCHARIN Artificial sweetener since its discovery in Manufactured by combining antralnalic acid with nitrous acid, sulfur dioxide, and ammonia. No nutritional value. Popular because it contains no calories, and does not add unwanted weight. 300 times sweeter than sugar. Derivative of coal tar. Despite this, saccharin was taken off of the carcinogenic list in 2010 by the FDA, and its safety is still debated.
4 BACKGROUND ON E. COLI Escherichia coli is a large and diverse group of gram (-) bacteria. Common symbiont in the digestive system of mammals. Causes several diseases if too much of it exists inside the intestines, or if it exist in the bloodstream. Standard prokaryotic model. Lab strain DH5 Alpha used as model bacterium.
5 PURPOSE To determine whether saccharin affects the survivorship of microbial flora, such as E. Coli.
6 HYPOTHESIS Null- Saccharin will have no significant effect on the survivorship of E. Coli. Alternate- Saccharin will significantly reduce survivorship on E. Coli.
7 MATERIALS Micropipettes Sterile pipette tips Saccharin, C7H5NO3S, (stock concentration 10%) Escherichia coli (DH5-Alpha) Sterile Dilution Fluid (100mM KH2PO4, 100mM K2HPO4, 10mM MgSO4, 1mM NaCl) LB Agar Plates LB Media (0.5% yeast extract, 1% tryptone, 1% sodium chloride) Spreader Labeling Tape Marker Vortex Test tube rack Sidearm flask Klett Spectrophotometer Ethanol Bunsen Burners Matches Latex gloves
8 PROCEDURE 1. E. coli was grown overnight in sterile LB media. 2. A sample of this culture was added to LB media in a sidearm flask. 3. The culture was incubated until a density of 50 Klett spectrophotometer units was reached. This represents a density of 10^8 cells/ml. 4. The culture was then diluted in a sterile dilution fluid to a density of 10^5 cells/ml. 5. Stock of Saccharin was pipetted out of the bottle (assumed to be sterile because of an non-broken seal) and was diluted to concentrations of 0%, 0.1%, 1%, and 10% in sterile dilution fluid.
9 CONCENTRATION CHART Concentration 10% 1% 0.1% 0% Sterile Dilution Fluid 8.9mL 9.8mL 9.89mL 9.9mL E. Coli.1mL.1mL.1mL.1mL Saccharin Solution 1mL.1mL.01mL 0mL Total Volume 10mL 10mL 10mL 10mL
10 PROCEDURE (CONTINUED) mL of cell culture was then added to the test tubes, yielding a final volume of 10mL and had a cell density of approximately 10^3 cells/ml/ 7. The solution was mixed by vortexing. 8. After vortexing to evenly suspend cells, 0.1 aliquots were removed from the tubes and spread on LB agar plates. 9. The plates were incubated at 37 degrees Celsius for 24 hours. 10. The resulting colonies were counted. Each colony is assumed to have risen from one cell.
11 DATA CHART Survivorship Saccharin 10% Saccharin 1% Saccharin.1% Saccharin 0% Trial One Trial Two Trial Three Trial Four Trial Five Trial Six Average
12 Colonies EFFECTS OF SACCHARIN ON E. COLI P Value: 4e Colonies Saccharin 0% Saccharin Saccharin.1% 1% Percent Saccharin Saccharin 10%
13 Colonies EFFECTS OF SACCHARIN ON E. COLI 1400 P Value: 4e Average Saccharin 0% Saccharin.1% Saccharin 1% Saccharin 10% Percent Saccharin
14 DUNNETT S TEST RESULTS Results T-Value T-Critical Variation 10% Saccharin Significant 1% Saccharin Significant 0.1% Saccharin Significant
15 CONCLUSION Null Hypothesis Rejected: Saccharin did decrease survivorship of E. Coli at all concentrations.
16 LIMITATIONS Synchronization when spreading plates was not exact. Only one species of bacteria was tested. Some cells could have had longer or shorter exposure times. Only survivorship was tested.
17 EXTENSIONS More trials to create a better basis for evaluating the results. Using sucralose or aspartame instead of saccharin. Testing a gram (+) species of bacteria, such as Staphylococcus epidermidis. Prolonged exposure test using saccharin infused into the agar. Using an antibiotic resistant strain of bacteria for the test model. A growth curve could be performed.
18 REFERENCES 1. in-danger-side-effects/
19 ANOVA Anova: Single Factor SUMMARY Groups Count Sum Average Variance Column Column Column Column ANOVA Source of Variation SS df MS F P-value F crit Between Groups E Within Groups Total
20 DUNNETT S TESTS Column 1 Average of Variable Average of Control Mean Squred Within Groups Replicates Resulting T-value Column 2 Average of Variable Average of Control Mean Squared Within Groups Replicates Resulting T-value Column 3 Average of Variable Average of Control Mean Squred Within Groups Replicates Resulting T-value
Hydrogen Peroxide Influence on Microbial Survivorship. Jacob Cebulak Central Catholic Pittsburgh Grade 9
Hydrogen Peroxide Influence on Microbial Survivorship Jacob Cebulak Central Catholic Pittsburgh Grade 9 Problem Humans use excess hydrogen peroxide to clean wounds. The concentration used is often damaging
More informationThe Effects of Shampoo on Microbial Flora. Andrew Walker Grade 9 Central Catholic High School
The Effects of Shampoo on Microbial Flora Andrew Walker Grade 9 Central Catholic High School Shampoo Hair care product used to clean hair of unwanted build up Combined soap, water, and herbs to make hair
More informationEffects of Ethyl Alcohol on Microbial Survivorship. Tim Olson 9th Grade Central Catholic High School
Effects of Ethyl Alcohol on Microbial Survivorship Tim Olson 9th Grade Central Catholic High School Ethyl Alcohol Pure alcohol or drinking alcohol Oldest recreational drug Alcohol intoxication Inhibits
More informationArtificial Sweetener Effects on Microbial Flora. Thomas Peilert Central Catholic High School
Artificial Sweetener Effects on Microbial Flora Thomas Peilert Central Catholic High School Problem Can the artificial sweetener Equal Original affect survivorship of Escherichia coli? Microbial Flora
More informationSynergistic Effects of Vitamin B12 and Creatine on Microbes. Jake Rocchi CCHS, 11th grade 2nd year in PJAS
Synergistic Effects of Vitamin B12 and Creatine on Microbes Jake Rocchi CCHS, 11th grade 2nd year in PJAS Vitamin B12 Water-soluble vitamin Needed for proper functioning in brain, nervous system, and blood
More informationThe Effects of Alcohol and Nicotine on Microbial Flora. Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School
The Effects of Alcohol and Nicotine on Microbial Flora Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School Microbial Flora The internal and external flora has eukaryotic fungi, protists, and
More informationBlair Bean Grade 9 Pittsburgh Central Catholic Highschool
Blair Bean Grade 9 Pittsburgh Central Catholic Highschool Variety of organisms that live in and on the body Skin Gastrointestinal tracts Nose Pharynx Mouth Conjunctiva Provide moisture, nourishment, and
More informationAntimicrobial Effects of Vinegar. Daniel Crawford Grade 9 Central Catholic High School
Antimicrobial Effects of Vinegar Daniel Crawford Grade 9 Central Catholic High School Antibacterial Agents Humans interest in microbial growth/survivorship for over 100 years Many products/technologies
More informationTHE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL
THE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL PROBLEM Do sports drinks have adverse effects on microflora cells? HUMAN SYMBIONTS, PATHOGENS,
More informationVitamin C and Ibuprofen Effects on Escherichia Coli. Timothy Leisenring Grade 11 Central Catholic High School
Vitamin C and Ibuprofen Effects on Escherichia Coli Timothy Leisenring Grade 11 Central Catholic High School Rationale for Experiment Commonly, ingested materials are investigated for effects on human
More informationChlorine and UV Effects on Bacteria Survivorship. Oscar Shaver Grade 9 Central Catholic High School
Chlorine and UV Effects on Bacteria Survivorship Oscar Shaver Grade 9 Central Catholic High School Electromagnetic Spectrum Diagram showing the range of all types of frequencies of electromagnetic radiation
More informationBy: Stephen Pfau Pittsburgh Central Catholic High School Grade 9
By: Stephen Pfau Pittsburgh Central Catholic High School Grade 9 Everyday thousands of people take cold/flu medicine and pain relief medicine Two common medicines for cold/flu and pain are DayQuil (cold)
More informationThe Effects of Reiki on Bacteria Survivorship. Jordan Ciccone Central Catholic High School
The Effects of Reiki on Bacteria Survivorship Jordan Ciccone Central Catholic High School Reiki Japanese spiritual healing practice meaning universal life energy. Training is done by a Reiki Master. Either
More informationBY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL
BY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL INTRODUCTION Chicken is one of the most consumed meats in the world, though can pose health risks (salmonella). Salmonella was thought only
More informationNIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION
NIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION MUSIC Elements: pitch, rhythm, timbre, texture, and dynamics Different types of music can exclude and include certain
More informationBill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11
Bill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11 The technical term for saltiness in aquatic environments is halinity, from the fact that halides, or chloride, are the most abundant anions in
More informationAmoeba Therapy on Streptococcus Pneumonia. By Sam Casale
Amoeba Therapy on Streptococcus Pneumonia By Sam Casale Microbial Therapy A lesser used form of therapy. Involves using microorganisms to fight other microorganisms. Phage Therapy is the most commonly
More informationThe Effects of Spray Sunscreen on MG63 Cancer Cell Lines. Anthony Williams Pittsburgh Central Catholic High School Grade 11
The Effects of Spray Sunscreen on MG63 Cancer Cell Lines Anthony Williams Pittsburgh Central Catholic High School Grade 11 Sunscreen Meant to protect against harmful UV rays that can be carcinogenic Comes
More informationRecipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones)
Protocol: 300 ml Yeast culture preparation Equipment and Reagents needed: Autoclaved toothpicks Shaker Incubator set at 30 C Incubator set at 30 C 60 mm 2 sterile petri dishes Autoclaved glass test tubes
More informationEvaluation of Antibacterial Effect of Odor Eliminating Compounds
Evaluation of Antibacterial Effect of Odor Eliminating Compounds Yuan Zeng, Bingyu Li, Anwar Kalalah, Sang-Jin Suh, and S.S. Ditchkoff Summary Antibiotic activity of ten commercially available odor eliminating
More informationBiological Consulting Services
Biological Consulting Services of North Florida/ Inc. May 13, 2009 Aphex BioCleanse Systems, Inc. Dear Sirs, We have completed antimicrobial efficacy study on the supplied Multi-Purpose Solution. The testing
More informationO. Repeat the measurement in all relevant modes used in your experiments (e.g. settings for orbital averaging).
Before You Begin Read through this entire protocol sheet carefully before you start your experiment and prepare any materials you may need. This year, in order to improve reproducibility, we are requiring
More informationCalibration Protocols
Calibration Protocols (1)OD 600 Reference point LUDOX Protocol Materials: 1ml LUDOX CL-X (provided in kit) ddh20 (provided by team) 96 well plate, black with clear flat bottom preferred (provided by team)
More informationBailey Abney and Brendan Kelley. Macomb Mathematics Science and Technology Center. AP Chemistry 12. Mrs. Hilliard, Mrs. Gravel, Mr. Acre, Mrs.
Comparing the Effects of Artificial Sweeteners and Sugar on the Growth Rate of Lactobacillus Acidophilus Bailey Abney and Brendan Kelley Macomb Mathematics Science and Technology Center AP Chemistry 12
More informationPreparation of Penicillins by Acylation of 6-Aminopenicillanic acid with Acyl Chlorides Week One: Synthesis
Preparation of Penicillins by Acylation of 6-Aminopenicillanic acid with Acyl Chlorides Week One: Synthesis Wear gloves during this experiment. Dissolve 1.05g of sodium bicarbonate in 12mL of acetone:
More informationMicroplate Nutrient Analysis: NH 4 +, NO 3 -, PO 4
Microplate Nutrient Analysis: NH 4 +, NO 3 -, PO 4 3+ Making Solutions: It is helpful to make the reagents and the concentrated (100 or 50 ppm) stock solution the day before running samples. When you are
More informationProduct Use HPSC-CC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
HPSC-derived Cardiomyocyte Cells (HPSC-CC) Catalog #6240 Cell Specification Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity
More informationDetermination of MIC & MBC
1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight
More informationCoenzyme Q-10 Effects on Mammalian Cell Behavior. Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013
Coenzyme Q-10 Effects on Mammalian Cell Behavior Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013 An Overview of Stem Cells Unspecialized cells capable of renewing themselves through
More informationASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS
Lucrări ştiinńifice Zootehnie şi Biotehnologii, vol. 41(1) (2008), Timişoara ASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS TESTAREA INFLUENłEI SISTEMELOR
More informationA Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance
A Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance Abstract Outbreaks of Salmonella contamination of peanut butter have become a serious food safety concern for
More informationTKB1 Competent Cells. Instruction Manual. Research Use Only. Not for Use in Diagnostic Procedures. Catalog # Revision B
TKB1 Competent Cells Instruction Manual Catalog #200134 Revision B Research Use Only. Not for Use in Diagnostic Procedures. 200134-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement
More informationEXERCISE. Proteins,Amino Acids, and Enzymes VII: Oxidase Test. Suggested Reading in Textbook. Pronunciation Guide. Materials per Student
EXERCISE 30 Proteins,Amino Acids, SAFETY CONSIDERATIONS Be careful with the Bunsen burner flame. No mouth pipetting. The oxidase reagent is caustic. Avoid contact with eyes and skin. In case of contact,
More informationScreening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal
Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Article Summary (In short - What is your article about Just 2 or 3 lines) Category: Bacillus sp produce
More informationMicrobiological Methods V-A- 1 SALMONELLA SPECIES PRESUMPTIVE AND CONFIRMATION TESTS
Microbiological Methods V-A- 1 PRESUMPTIVE AND CONFIRMATION TESTS PRINCIPLE SCOPE Enrichment and selective procedures are used to provide a reasonably sensitive, definitive and versatile means of qualitatively
More informationG/LITRE 5.0 g KOH g 0.5 g 0.05 g 0.01 g MgS047H20 NaCl CaCl2
A P P E N D IX -V III COMPOSITION OF USED MEDIA AND CHEMICAL REAGENTS 1. NITROGEN FREE BROMOTHYMOL BLUE (NFB) MEDIUM Dobereiner et al (1976) Same media was also used to check the effect of temperature
More informationMouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and
More informationEXPERIMENT. Bacteria Identification through Functional Media Motility Testing
EXPERIMENT Bacteria Identification through Functional Media Motility Testing Hands-On Labs, Inc. Version 42-0248-00-02 Review the safety materials and wear goggles when working with chemicals. Read the
More informationMALDI Sepsityper Kit
Instructions for Use MALDI Sepsityper Kit Kit for identification of microorganisms from positive blood cultures using the MALDI Biotyper system CARE products are designed to support our worldwide customers
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE
Translated English of Chinese Standard: GB4789.30-2016 www.chinesestandard.net Buy True-PDF Auto-delivery. Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.30-2016
More informationEXPERIMENT. Bacteria Identification through Functional Media Motility Testing
EXPERIMENT Bacteria Identification through Functional Media Motility Testing Hands-On Labs, Inc. Version 42-0248-00-02 Review the safety materials and wear goggles when working with chemicals. Read the
More informationWilliams Lab Recipes ANTIBIOTICS
Williams Lab Recipes ANTIBIOTICS 1000x Ampicillin (sodium salt) 100mg/ml recipe 1. Measure out 1 g of Ampicillin tri hydrate 2. Add Milli-Q H2O to 10 ml 3. Add ~.1 g of NaOH pellets (half pellet or more
More informationLaboratory Protocol. November 2017 Version 3. Henrik Hasman, Yvonne Agersø and Lina M Cavaco (DTU Food)
Laboratory Protocol Validation of selective MacConkey agar plates supplemented with 1 mg/l cefotaxime for monitoring of ESBL- and AmpCproducing E. coli in meat and caecal samples November 2017 Version
More informationTOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS. Objective To make a serial dilution for use in dose/response bioassays.
TOXICOLOGY PROTOCOLS PROTOCOL 1. SERIAL DILUTIONS Objective To make a serial dilution for use in dose/response bioassays. Background The idea behind a bioassay is that the test organism will respond in
More informationEFFECTS OF NICOTINE ON HUMAN MESENCHYMAL STEM CELLS. Connor McNeil Central Catholic HS
EFFECTS OF NICOTINE ON HUMAN MESENCHYMAL STEM CELLS Connor McNeil Central Catholic HS Purpose To determine whether nicotine causes any effects on human Mesenchymal Stem Cell (hmsc) proliferation or migration
More informationSurvival of Aerobic and Anaerobic Bacteria in
APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,
More informationAppendix A: Preparation of Media and Chemicals. Malt Extract Agar (MEA) weighing g was dissolved in 400 ml of distilled water
Appendix A: Preparation of Media and Chemicals Preparation of Malt Extract Agar (MEA) Malt Extract Agar (MEA) weighing 13.44 g was dissolved in 400 ml of distilled water in an Erlenmeyer flask using a
More informationCommercial in Confidence
Determination of Bactericidal Activity of Zwiteck Sanitizer using the European Standard Method BS EN 1276:2009 ~ Project Report Prepared for Zwiteck BV Inspiring tomorrow s professionals Determination
More information1.1. Gelatinizing Plates Prepare plates by covering surface with 0.1% Gelatin solution:
1. Preparation of Feeder Layers And SNL Stocks 1.1. Gelatinizing Plates Prepare plates by covering surface with 0.1% Gelatin solution: Plate Size (cm) 3 2 6 4 10 9 15 18 Amount of Gelatin (ml) Swirl the
More informationESSENTIAL OILS & The MICROBIOME
ESSENTIAL OILS & The MICROBIOME BAHAR MAHMOUDI CONTENTS - The Human Microbiome - Benefits of the Normal Flora - Microbiome Improvement - Essential Oils 1 What is Human Microbiome Microbe: Tiny living organism,
More informationMTS assay in A549 cells
Project: VIGO MTS assay in A549 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
More informationCarbon dioxide production of wild type and PDC1 mutant Saccharomyces cerevisiae in D-glucose
Carbon dioxide production of wild type and PDC1 mutant Saccharomyces cerevisiae in D-glucose Luke Gooding, Grace Lam, Simran Parmar, Jessica Sham Abstract To study the differences in respiration between
More informationPenetration of Sodium Lauryl Sulfate in Gallus Gallus Domesticus Egg Shell. Thousand Oaks High School AP Research STEM
Penetration of Sodium Lauryl Sulfate in Gallus Gallus Domesticus Egg Shell Thousand Oaks High School AP Research STEM Overview Detecting sodium lauryl sulfate through high performance liquid chromatography
More informationMETABOLIC INJURY TO BACTERIA AT LOW TEMPERATURES
METABOLIC INJURY TO BACTERIA AT LOW TEMPERATURES ROBERT P. STRAKA AND J. L. STOKES Western Regional Research Laboratory,' Albany, California Received for publication January 19, 1959 The death of bacteria
More informationComparison of a Waterless, Scrubless CHG/Ethanol Surgical Scrub to Traditional CHG and Povidone-Iodine Surgical Scrubs
Comparison of a Waterless, Scrubless CHG/Ethanol Surgical Scrub to Traditional CHG and Povidone-Iodine Surgical Scrubs by M. Sigler 1 J. Bastyr 2 J. Stahl 2 J. Pyrek 2 1 Stephens and Associates, Inc.,
More information--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB5009.
Translated English of Chinese Standard: GB5009.259-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD GB OF THE PEOPLE S REPUBLIC OF CHINA National food safety standard Determination
More informationAPPENDIX A Media and Solution Preparation
APPENDIX A Media and Solution Preparation Appendix A Regarding the present experimental research, a summary of recommended and standard aseptic techniques should be followed throughout every procedure,
More informationMedia Fill Test Kits. Manufactured by. Making USP <797> compliance easy!
Media Fill Test Kits Manufactured by Making USP compliance easy! Compliance was never so easy! Microbial Contamination Testing for CSPs Hardy Diagnostics offers all the products you need to easily
More informationAdenovirus Manual 1. Table of Contents. Large Scale Prep 2. Quick MOI Test 4. Infection of MNT-1 Cells 8. Adenovirus Stocks 9
Adenovirus Manual 1 Table of Contents Large Scale Prep 2 Quick MOI Test 4 TCID 50 Titration 5 Infection of MNT-1 Cells 8 Adenovirus Stocks 9 CAUTION: Always use filter tips and bleach everything!!! Adenovirus
More information(LM pages 91 98) Time Estimate for Entire Lab: 2.5 to 3.0 hours. Special Requirements
Laboratory 7 Chemical Aspects of Digestion (LM pages 91 98) Time Estimate for Entire Lab: 2.5 to 3.0 hours Special Requirements Incubation. Students should start these sections at the beginning of the
More informationAntibiotic Resistance Lab Report. Bacterial plasmids are small, circular molecules of DNA that can be found in
Adil Sabir Bio 110H 24 November 2014 Antibiotic Resistance Lab Report I. Introduction Bacterial plasmids are small, circular molecules of DNA that can be found in bacteria (Hass, Richter, Ward, 2014).
More informationHigher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).
4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine
More informationLaboratory 10 Factors Controlling Phagocytosis in Tetrahymena,
BIO354: Cell Biology Laboratory 1 Laboratory 1 Factors Controlling Phagocytosis in Tetrahymena, I. Introduction A characteristic feature of all eukaryotic cells is the ability to pinch off portions of
More informationß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose
ß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose JILLIAN CLARK, JACQUIE HUDSON, ROBIN MAK, CHRISTA McPHERSON, AND CARMEN TSIN Department of Microbiology
More informationBACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION. SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE
BACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE slaramo1@fau.edu 772 242 2525 Spread Plate Method This is the easiest and most common plating
More informationCount. Enterobacteriaceae. Interpretation Guide. 3M Food Safety 3M Petrifilm Enterobacteriaceae Count Plate
3M Food Safety 3M Petrifilm Enterobacteriaceae Count Plate Enterobacteriaceae Count Interpretation Guide This guide familiarizes you with results on 3M Petrifilm Enterobacteriaceae Count Plates. For more
More informationMALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology
MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology Patrick R. Murray, PhD WW Director, Scientific Affairs BD Diagnostic Systems Outline MALDI-TOF is the most important innovation
More informationComparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor
Comparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor M. G. Sanal Kumar, S. Nandakumar, B. Bini & Arya Raj R. S. P.G. & Research Department of Zoology,
More information(a) (i) Structures A and B are found in both the animal cell and the bacterial cell. B... (2)
1 The diagrams show an animal cell and a bacterial cell. (a) (i) Structures A and B are found in both the animal cell and the bacterial cell. Use words from the box to name structures A and B. cell membrane
More informationBACTERIAL EXAMINATION OF WATER
BACTERIAL EXAMINATION OF WATER The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing,
More informationThe Effect of Epinephrine on Cancer Cell Proliferation. Alec Chrystal Grade 11 Central Catholic High School PJAS 2019
The Effect of Epinephrine on Cancer Cell Proliferation Alec Chrystal Grade 11 Central Catholic High School PJAS 2019 Stress is the feeling of physical, social, or emotional pressure Struggling to meet
More informationLab 4. Blood Culture (Media) MIC AMAL-NORA-ALJAWHARA 1
Lab 4. Blood Culture (Media) 2018 320 MIC AMAL-NORA-ALJAWHARA 1 Blood Culture 2018 320 MIC AMAL-NORA-ALJAWHARA 2 What is a blood culture? A blood culture is a laboratory test in which blood is injected
More informationSOP History Number Date Reason for Change v1 10/10/2014 Original V2 10/10/2016 Update V3 10/10/2018 Update
Document Number: SASoM/METHOD/073.v3 Title: Version: Author: Lentivirus production from HEK293T cells v3 Paul Reynolds Effective from: 10/10/2018 Valid to: 09/10/2020 SOP History Number Date Reason for
More informationImpact of Sodium Reduction on Survival of Listeria monocytogenes in Sliced Process Cheese
Impact of Sodium Reduction on Survival of Listeria monocytogenes in Sliced Process Cheese July 2013 By: Dr. Francisco Diez Gonzalez University of Minnesota Dr. Mastura Akhtar Partners: Midwest Dairy Association
More informationHow to interpret your urine sample results
How to interpret your urine sample results Chronic UTI Info Factsheet Series Once you have submitted your urine sample for analysis, it will be sent off to the local laboratory or hospital laboratory if
More informationLab #9. Introduction. Class samples:
Lab #9 Introduction Food-borne illness is largely caused by the presence of bacteria in red meat. However, much of these harmful bacteria can be destroyed and prevented by sanitation and safe cooking practices.
More information2-Deoxyglucose Assay Kit (Colorimetric)
2-Deoxyglucose Assay Kit (Colorimetric) Catalog Number KA3753 100 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationProtocols for Laboratory Verification of Performance of the BioFire FilmArray Pneumonia Panel
Protocols for Laboratory Verification of Performance of the BioFire FilmArray Pneumonia Panel Laboratory Protocols for Use with a ZeptoMetrix NATtrol Verification Panel Purpose The Clinical Laboratory
More informationNINDS Repository Human Induced Pluripotent Stem Cell (ipsc) Handling Protocols (Matrigel and mtesr Media)
General Guidelines for Handling Human ipsc cells ipsc are cryopreserved in plastic cryovials and shipped on dry ice. If storing the ipsc before thawing, store in liquid nitrogen vapor. Storage directly
More informationHow to interpret your urine sample results
How to interpret your urine sample results Chronic UTI Info Factsheet Series Once you have submitted your urine sample for analysis, it will be sent off to the local laboratory or hospital laboratory if
More informationResearch Article. The effects of hyaluronic acid on the morphological physiological differentiation of Lactobacillus
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(7):368-372 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 The effects of hyaluronic acid on the morphological
More informationMorinaga Mouse C-peptide ELISA Kit
Morinaga Mouse C-peptide ELISA Kit For the quantitative determination of C-peptide in mouse serum, plasma, and fluid 96wells For Laboratory Use Only, not for use in diagnostic procedure Please read full
More informationEXPERIMENT. Food Safety
EXPERIMENT Food Safety Hands-On Labs, Inc. Version 42-0308 -00-01 Review the safety materials and wear goggles when working with chemicals. Read the entire exercise before you begin. Take time to organize
More informationthe following objectives: 1. Describe the environment of the stomach and its relation to protein digestion.
Unit B: Understanding Animal Body Systems Lesson 5: Protein Digestion by Enzyme Action Student Learning Objectives: Instruction in this lesson should result in students achieving the following objectives:
More informationIV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use.
Definitions Instructions for use REF Catalogue number IV2-113E Use by English Invitron Glargine ELISA Kit For in-vitro diagnostic use Σ 96 LOT IVD Lot/Batch Code Storage temperature limitations In vitro
More informationAppendix 3 Media and solutions
Appendix 3 Media and solutions 1. Buffer solution Phosphate buffered saline (PBS) Recipe to prepare five litres of PBS Reagents Sodium chloride NaCl 40.0g Potassium chloride KCl 1.0g Potassium dihydrogen
More informationTHE MOUTHWASH CHALLENGE
THE MOUTHWASH CHALLENGE Ginny Brown Churchill HS / Montgomery Co. MD Abstract A problem assignment to design a new mouthwash that will combat the growth of bacteria that is frequently blamed for gum disease.
More informationTechnical Procedures and Handling
Mbio-II-Handling X/1 08.12.99 Technical Procedures and Handling 1 Autoclave HiClave (Wolf) HA-300MII Purpose: Sterilization of culture media with autoclave. Procedure: Hiclave Wolf HA-300MII: Before it
More informationBurping Yeast: An Investigation of Cellular Respiration
Burping Yeast: An Investigation of Cellular Respiration Student Materials Introduction... 2 Lab Protocol... 4 Data Collection Worksheet... 6 Pre-Lab Questions... 7 Post-Lab Questions and Analysis... 8
More informationCore practical 14: Investigate the effect of gibberellin on the production of amylase in germinating cereals using a starch agar assay
Core practical 14 Teacher sheet Core practical 14: Investigate the effect of gibberellin on the production of amylase in germinating cereals using a starch agar assay Objectives To investigate the effect
More informationB. 15 mm Ouabain Solution (Ouabain) (Prepare 10 ml in Reagent A using Ouabain Octahydrate, Sigma Prod. No. O3125.)
SIGMA QUALITY CONTROL TEST PROCEDURE Sigma Prod. No. A7510 PRINCIPLE: ATP + H 2 O ATPase > ADP + P i Abbreviations used: ATPase = Adenosine 5'-Triphosphatase ATP = Adenosine 5'-Triphosphate ADP = Adenosine
More informationThe Effects of L-glutamate, L-glutamine, and L-aspartic Acid on the Amylase Production of E. coli Transformed with pamylase
The Effects of L-glutamate, L-glutamine, and L-aspartic Acid on the Amylase Production of E. coli Transformed with pamylase Kevin S. Liu and Brittany Franckowiak Wilde Lake High School, Howard County,
More informationYou Are What You Eat: An Investigation of Macromolecules
You Are What You Eat: An Investigation of Macromolecules Teacher Materials Students will use standard indicators to test carbohydrates, lipids, proteins, and nucleic acids. Learning Goals, Objectives,
More informationVEIKKO NURMIKKO. in which the organisms under investigation are separated from each other by one or more dialysis
Microbiological Determination of Vitamins and Amino Acids Produced by Microorganisms, Using the Dialysis Cell1 VEIKKO NURMIKKO Laboratory of Valio, Biochemical Institute, Helsinki, Finland Received for
More informationIntroduction to Microbiology BIOL 220, Summer Session 1, 1996 Exam # 2
Name I. Multiple Choice (1 point each) Introduction to Microbiology BIOL 220, Summer Session 1, 1996 Exam # 2 D 1. Which transport process requires energy? A. Osmosis C. Diffusion B. Facilitated diffusion
More informationHOW TO SOLVE PRACTICAL ASPECTS OF MICROBIOLOGY
HOW TO SOLVE PRACTICAL ASPECTS OF MICROBIOLOGY PROPOSAL: NEW EXERCISES PART 2 Inés Arana, Maite Orruño & Isabel Barcina Department of Immunology, Microbiology and Parasitology University of Basque Country
More informationFatty Acid Oxidation Assay on the XF24 Analyzer
Fatty Acid Oxidation Assay on the XF24 Analyzer Mitochondria oxidize a variety of fuels to generate ATP through oxidative phosphorylation. Cells can utilize fatty acid, glucose and amino acids as their
More informationBiodiversity Study & Biomass Analysis
PR072 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biodiversity Study & Biomass Analysis Teacher s Guidebook (Cat. # BE-403) think proteins!
More informationBIOLOGY EXPERIMENT STEP 1: Symptom Chart Diabetes Mono Strep Throat
BIOLOGY EXPERIMENT When the students arrive, please have them sit at a table, put on their lab coats and safety glasses. For the next 30 minutes they are going to be Medical Laboratory Technicians. They
More informationMouse Leptin ELISA Kit Instructions
V.6/Mar/2008 CRYSTAL CHEM INC. Mouse Leptin ELISA Kit Instructions For the quantitative determination of leptin in mouse serum or plasma and fluid Catalog #: 90030 96 Assays For Research Use Only. Not
More information