Essential Question: How do living things inherit their genetic characteristics?
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1 Essential Question: How do living things inherit their genetic characteristics? Activity 6 Analyzing Genetic Data Purpose: To learn how to predict the outcome of genetic crosses with s Instructions: Follow the directions step by step to predict the outcome of genetic crosses. Vclip Reference: Backgound: A team of scientist buys two Beebop terriers to study. One is a male, Ocean, the other a female, Lucy. The scientists are interested in analyzing inheritance patterns in Beebops tail color. The scientists want to mate Ocean and Lucy. They know that Ocean and Lucy have heterozygous traits for tail color. In other words their genotype is Tt. Their tails are both blue = the phenotype. Here is a summary of all the possible Beebop tail color genotypes and phenotypes: Data Table One: Summary of Beebop tail color data & terminology Characteristic Traits for Tail Color Genotypes Phenotypes Type of gene combination Tail Color T, t T = Blue t = Orange TT = Tt = tt = Blue tail color Blue tail color Orange tail color TT = Homozygous Dominant Tt = Heterozygous tt = Homozygous Recessive A. Help the scientists by completing a below and predict the possible results of this experiment. Use this 5 step procedure for each analysis. 1. Determine the alleles present in the male & female. Write these by the Beebop. 2. Prepare for Mating. Create the possible sperm and egg combinations by separating both alleles Write each allele on the spaces next to the. 3. Mate! Write each allele in the two boxes either below or beside sperm and egg spaces. Be sure to write the capital letter first. (Tt not tt!) 4. Collect data: Add up the like combinations (TT, Tt, or tt) and write the totals in the analysis section. 5. Analyze: Calculate the percentage of each total by dividing each total by the total number of squares.
2 B. The scientists are really excited about the results and have learned a lot about Beebop Terrier heredity! Now the scientists want to find out what will happen if they cross breed: the male offspring with the homozygous recessive genotype for Orange tails (tt) and the female offspring who have heterozygous genotype for Blue tails (Tt). C. These were unexpected results! Our scientists need to learn a lot more about breeding Beebop Terriers. So the scientists decide that they need to do another experiment where they cross: the male offspring with the homozygous dominant genotype for Blue tails (TT) and the female offspring that have heterozygous Blue tails (Tt).
3 Essential Question: How do living things inherit their genetic characteristics? Instructions: Complete the next four genetic cross problems to determine the genotypes of Fruit Fly offspring across five generations. Be sure to answer questions 1-3 thoroughly afterwards. Cross 1. Homozygous Dominant to Homozygous Recessive Second Generation offspring analysis. A Homozygous Dominant male Red-Eyed fruit fly (RR) crosses with a Homozygous female White-Eyed fruit fly (rr) Red eye is dominant White eye trait is recessive. According to Mendel, each gene is randomly segregated. Each fruit fly has two genes, which separate into different gametes: Male gamete is a sperm cell Female gamete is an egg cell 1. Write the genotype for the male and female fruit flies on the lines. 2. Then write the alleles for each gamete (sperm & egg) in the proper spaces. 3. Show the results of each cross in the Punnett square. 4. Show the results of this cross as a ratio of red-eyed homozygous (RR), to red-eyed heterozygous (Rr do not write rr), to white-eyed homozygous (rr). Red Eye (RR) Red Eye (Rr) White Eye (rr) Homozygous D Heterozygous Homozygous D Phenotype Ratio: Red : White Cross 2. Heterozygous to Heterozygous Third Generation offspring analysis Next let s cross the offspring (Rr) from the above experiment (the second generation) with each other to produce a 3 rd generation. 1. In this second cross, let s assume that two of the offspring from Cross 1. were to mate (be crossed). 2. Both offspring are heterozygous for the eye color characteristic. 3. Please complete steps 1-4 from Cross 1 for this combination on the Punnett square to the right. ( Use Rr do not write rr), to white-eyed homozygous (rr). Red Eye (RR) Red Eye (Rr) White Eye (rr) Homozygous D Heterozygous Homozygous D Phenotype Ratio: Red : White
4 Fourth Generation offspring analysis Cross 3. Homozygous Dominant to Heterozygous Let s cross some of the offspring of the third generation (cross 2) above. 1. Cross a male homozygous dominant fruit fly with a female heterozygous fruit fly. 2. Next, please complete steps 1-4 from Cross 1 for this combination on the Punnett square to the right. Red Eye (RR) Red Eye (Rr) White Eye (rr) Homozygous D Heterozygous Homozygous D Phenotype Ratio: Red : White Cross 4. Homozygous Recessive to Heterozygous Fifth Generation Offspring analysis Finally, let s cross a male homozygous recessive (rr) fruit fly with a female heterozygous fruit fly (Rr) from the fourth generation (cross 3) above. 1. Each fruit fly has two alleles for the eye color gene so separate these into different gametes - male to sperm cell and female to the egg cell. 2. Next, please complete steps 1-4 from Cross 1 for this combination on the Punnett square to the right. 3. Show the results of this cross as a ratio of red-eyed homozygous (RR), to red-eyed heterozygous (Use Rr do not write rr), to white-eyed homozygous (rr). Red Eye (RR) Red Eye (Rr) White Eye (rr) Homozygous D Heterozygous Homozygous D Phenotype Ratio: Red : White
5 EC/Challenge Analysis Questions: Answer the following questions in complete, quality, and correct sentences and examples using facts, reasons, evidence, and data from the crosses you ve performed. 1. Explain why, using a punnett square as an example and words to tell why it is impossible for offspring to show a recessive trait if one parent is homozygous (BB) for the dominant trait for eye color (BB). Explain here: : : Homozygous Heterozygous Homozygous Dominant BGH BGH Recessive BLUE Phenotype Ratio BGH : BLUE : : Homozygous Heterozygous Homozygous Dominant BGH BGH Recessive BLUE Phenotype Ratio BGH : BLUE 2. A scientist gets some unlabeled red-eyed fruit flies. The scientist knows that the red eye trait is dominant over the white eye trait. But she wants to find out if the fruit flies are homozygous or heterozygous for the red eye color. Determine using the s below, which type of cross (RR, Rr, or rr) she will have to use to tell if the red-eyed fruit flies are homozygous (RR), or heterozygous (Rr). What will you cross to determine if the Fruit flies are RR or Rr? Be specific. : : Homozygous Heterozygous Homozygous Dominant Red Red Recessive White Phenotype Ratio: RED : WHITE : : Homozygous Heterozygous Homozygous Dominant Red Red Recessive White Phenotype Ratio: RED : WHITE 3. Consider a population that started from a homozygous dominant X homozygous recessive cross. How often over the generations do you actually see a homozygous recessive trait, like white eyes, appearing? Hint : Look for a pattern in the punnett square results from Fruit fly Crosses 1-4.
6 Mendelian Genetics Let s use what we know to solve some fun genetics crosses!! Cross 1. Homozygous Dominant to Homozygous Recessive Second Generation offspring analysis. A Homozygous Dominant male Tall pea plant (TT) with a Homozygous short pep plant (tt) Tall is dominant Short is recessive. According to Mendel, each gene is randomly segregated. Each fruit fly has two genes, which separate into different gametes: Male gamete is a pollen cell Female gamete is an egg (ova) cell 1. Write the genotype for the male and female fruit flies on the lines. 2. Then write the alleles for each gamete (sperm & egg) in the proper spaces. 3. Show the results of each cross in the Punnett square. 4. Show the results of this cross as a ratio of Tall homozygous (TT), to Tall heterozygous (Tt do not write tt), to white-eyed homozygous (tt). Tall Pea (TT) Tall Pea (Tt) Short Pea (tt) Homozygous D Heterozygous Homozygous R Phenotype Ratio Tall : Short Cross 2. Heterozygous to Heterozygous Third Generation offspring analysis Next let s cross the offspring (Tt) from the above experiment (the second generation) with each other to produce a 3 rd generation. 1. In this second cross, let s assume that two of the offspring from Cross 1. were to mate (be crossed). 2. Both offspring are heterozygous for the eye color characteristic. 3. Please complete steps 1-4 from Cross 1 for this combination on the Punnett square to the right. (Tt do not write tt), to white-eyed homozygous (tt). Tall Pea (TT) Tall Pea (Tt) Short Pea (tt) Homozygous D Heterozygous Homozygous R Phenotype Ratio Tall : Short
7 Fourth Generation offspring analysis Cross 3. Homozygous Dominant to Heterozygous 1. Let s cross a: Homozygous Dominant Purple Flowered Pea (PP) with a Heterozygous Purple flowered Pea (Pp) Make the Heterozygous female. Note the Homozygous Recessive Pea (pp) is White Flowered. 2. Next, please complete steps 1-4 from Cross 1 for his combination on the Punnett square to the right. Purple (PP) Purple (Pp) White (pp) Homozygous D Heterozygous Homozygous R Phenotype Ratio Purple : White Cross 4. Homozygous Recessive to Heterozygous Fifth Generation Offspring analysis 1. Finally, let s cross a: Heterozygous Yellow Colored Pea (Yy) with a Homozygous Recessive Green Colored Pea (yy) Make the Homozygous female. 2. Next, please complete steps 1-4 from Cross 1 for this combination on the Punnett square to the right Yellow Pea (YY) Yellow Pea (Yy) Green Pea (yy) Homozygous D Heterozygous Homozygous R Phenotype Ratio Yellow : Green
8 Pedigree Puzzles. Puzzled? Refer to ACT 66 and vclip 1. Scientists are studying the orange tail trait and were worried when the trait was lost in the 2 nd generation. However this trait appeared in the 3 rd generation. (Assign your own letters) a. Explain how the information in this pedigree tells you whether orange tail color is dominant or recessive. b. Label each Beebop Terrier s genotypes in the figure below. 2. The figure below shows a pedigree of a family with PKU, or phenylketonuria. People with PKU can not break down protien normally. This leads to a buid up that leads to mental disability. If PKU is diagnosed just after birth a special diet is given to the affected individual until puberty that will avoid the build and the child is normal. a. Based upon the information below, is PKU likely to be a dominant or recessive trait? b. Label each individual s genotype in the figure below.
9 3. The figure below shows a family pedigree in which individuals are affected with Polydactyly, which causes individuals to have an extra finger or toe on each hand or foot. Individuals are otherwise completely normal. a. Is polydactyly likely to be a dominant or a recessive trait? Explain. b. Label each individual with the appropriate genotype on the figure below. (Assign your own letters) 4. The Pedigree shown below represents a Genetic Condition. (Assign your own letters) a. Is this Genetic Condition a Dominant Trait or a Recessive Trait? Explain. b. Is Sophia most likely homozygous dominant, heterozygous, or homozygous recessive? Explain.
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