PAIN PANEL. Application Guide. Screen 41 compounds in under 4 minutes

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1 Application Guide PAIN PANEL Screen 41 compounds in under 4 minutes Solutions for large compound screens and individual compound classes such as benzodiazepines, opiates, and more Achieve resolution of critical isobars Simplified, rapid cleanup procedures

2 Here to Support You with Your Clinical Research Personalized Support Your lab has its very own Technical Consultant that is available to assist with method development, optimization, troubleshooting, and product recommendations. Feel free to give them a call with any questions you may have! Get to Know Your Technical Consultant Take Advantage of Our Lab Our customers asked us for more support, we responded with PhenoLogix sm, a full-service analytical support laboratory. Let our PhenoLogix clinical research gurus save your lab time and money. Method development and optimization in our PhenoLogix laboratory On-site method development On-site hands-on training Explore PhenoLogix 24 Hour Access to Information Technical Database Access our vast database of technical notes, applications, webinars, and more. Search by industry, document type, and separation mode to easily find what you need. Tools Utilize our web tools to find product recommendations, create customized methods, and more. For Research Use Only. Not for use in diagnostic procedures. 214 Phenomenex, Inc. All rights reserved.

3 TABLE OF CONTENTS Simple Sample Preparation Solid Phase Extraction (SPE)...4- Protein Precipitation (PPT)...4- Full Pain Panel Screen Choose from 3 different core-shell stationary phases to customize your work Biphenyl, Greatest Versatility and Performance Phenyl-Hexyl, imethod Tested C18, General Purpose... 1 Analyze Individual Classes of Drugs Benzodiazepines Opiates Drugs of Abuse Ordering Information

4 Guaranteed Solutions Effective and highly reproducible methodology helps you ensure results and reduce costs. With guaranteed high quality products from Phenomenex, you can easily push your research to the next level. We guarantee that if Phenomenex products in this guide do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 4 days and keep the Phenomenex product for FREE! Solid Phase Extraction (SPE) Strata -X-Drug B Protein Precipitation (PPT) Impact Designed and quality controlled for basic drugs of abuse Time and solvent saving polymeric strong cation-exchange SPE sorbent No equilibration or condition steps needed Complete in plate removal of proteins (b-glucuronidase) Increase throughput 3x Leak-free oleophobic membrane Easily automated Part no. CE-766 HPLC/UHPLC Analysis Kinetex Biphenyl Core-Shell Technology Rugged and reliable core-shell columns Incredible performance and efficiencies TMS TMS Biphenyl Enhanced polar and basic retention Quality controlled for basic drugs of abuse 1.7 µm 2.6 µm µm 4

5 Rapid, Simple Cleanup for Pain Management Drugs and Drugs of Abuse Skip the method development, significantly reduce your sample preparation time, and save solvent using Strata -X-Drug B SPE tubes and 96-well plates. One method is all you will need to extract over 41 pain management drugs. Or easily upgrade the throughput of your existing protein precipitation methods with the new Impact Protein Precipitation long drip plates. Sample Pretreatment Acid Hydrolysis 1. To 2 ml urine sample, add µl concentrated hydrochloric acid. 2. Heat at 9 C for 2 hours. 3. Add 2 ml of 2 mm Sodium acetate buffer (ph 4.). 4. Add 1 ml of 6 N Potassium hydroxide (KOH). Vortex.. Centrifuge for -6 minutes at,-, rpm (2-22 C). 6. Verify ph of sample is between Load pretreated sample directly onto Strata-X-Drug B sorbent (see procedure below). Urine: Enzymatic Hydrolysis 1. Dilute µl urine with 1 µl buffer* and 2 µl of 1,, units/ml β-glucuronidase in a 96-well collection plate. 2. Vortex for -6 seconds. 3. Incubate in a water bath at 62 C for 3 minutes. 4. If performing SPE, transfer sample to a 96-well collection plate or autosampler vial, seal and centrifuge for 1 minutes at 2, rpm. Load supernatant onto Strata-X- Drug B sorbent (see procedure below.) If performing rapid removal of β-glucuronidase, load sample directly onto Impact Protein Precipitation plate (see procedure below). Serum / Plasma / Saliva: 1. Dilute plasma/serum 3:1 with 1 % Acetic acid. 2. Vortex. 3. Centrifuge for -6 minutes at 3,-3, rpm (2-22 C). 4. Load pretreated sample directly onto Strata-X- Drug B sorbent (see procedure below). *Buffer prepared by adding 8 ml deionized water and 111 ml glacial acetic acid to a 1 L volumetric flask, adjust final volume to the line with a % KOH solution and mix by inversion. For Ultra Clean and Concentrated Samples Strata-X-Drug B SPE Steps Procedure designed for 6 mg bed mass Strata-X-Drug B and can be adjusted for smaller or larger bed masses. Condition: Not required Equilibrate: Not required Load: Pretreated sample (see above for pretreatment recommendations) Wash: 2 ml of 1 mm Sodium acetate buffer (ph.) Wash 2: 2 ml Methanol Elute: 2 ml Ethyl acetate/isopropanol/ammonium hydroxide (ph.) (7:2:1) Evaporate: to dryness under a stream of Nitrogen at C Reconstitute: 1 ml 1 % Methanol Inject: μl Reduce solvent consumption; no conditioning or equilibration required! For Rapid Removal of Proteins (Ex. β-glucuronidase) Impact Protein Precipitation (PPT) Steps 1. Load 3 µl Acetonitrile to the wells of an Impact Plate 2. Load 1 µl hydrolyzed sample directly onto the Impact Plate 3. Vortex for 2 minutes at maximum possible speed 4. Apply vacuum at 2-7 inches Hg for 2-3 minutes and collect filtrate in a collection plate New long drip format now available! Part no. CE-766

6 Rapid, Reliable, and High Performance Pain Panel Testing Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl The new Kinetex Biphenyl ensures that your comprehensive screens display the needed performance with a ruggedness and reliability that will keep you at ease e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e. 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e 2.6 µm x 2.1 mm 2 bar min 2.6 µm min µm App ID x 3. mm 16 bar App ID 2214 x 3. mm 1 bar App ID 2212 Conditions for all separations: Columns: Kinetex 2.6 µm Biphenyl ( x 2.1 mm) Kinetex 2.6 µm Biphenyl ( x 3. mm) Kinetex µm Biphenyl ( x 3. mm) Mobile Phase: A:.1 % Formic Acid in Water B:.1 % Formic Acid in Methanol Gradient: Time (min) % B Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Samples: 1. Morphine 2. Oxymorphone 3. Hydromorphone 4. Amphetamine. Methamphetamine 6. MDA 7. Naloxone 8. Codeine 9. 6-MAM 1. Oxycodone 11. MDMA 12. Naltrexone 13. Hydrocodone 14. MDEA 1. Norfentanyl 16. Tramadol 17. Benzoylecgonine 18. Meprobamate 19. Normeperidine 2. Meperidine 21. Norbuprenorphine 22. Fentanyl 23. Buprenorphine 24. Flurazepam 2. PCP 26. Carisoprodol 27. Propoxyphene 28. Midazolam 29. Sufentanil 3. Norpropoxyphene 31. EDDP 32. Lorazepam 33. Methadone 34. Clonazepam 3. Oxazepam 36. Hydroxyalprazolam 37. Nordiazepam 38. Flunitrazepam 39. Temazepam 4. Alprazolam 41. Diazepam min

7 Go Even Faster! Using the high performance and versatility of the Kinetex 2.6 µm Biphenyl with a ballistic gradient allows you to cut down even more time from your cycles and significantly improve productivity. Imagine what you can do with this solution on your multiplex or dual-stream systems e6.e6 4.8e6 4.6e6 4.4e6 4.2e6 4.e6 3.8e6 3.6e6 3.4e6 3.2e6 3.e6 2.8e6 2.6e6 2.4e6 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e Kinetex 2.6 µm Biphenyl x 3. mm min App ID Conditions: Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic Acid in Water B:.1 % Formic Acid in Methanol Gradient: Time (min) % B Flow Rate:.7 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) Morphine Oxymorphone Hydromorphone Amphetamine Naloxone Methamphetamine Codeine MDA Oxycodone Naltrexone Hydrocodone MDMA MDEA Norfentanyl Tramadol Benzoylecgonine Meperidine Meprobamate Norbuprenorphine Fentanyl Buprenorphine Flurazepam Analyte Q1 Q3 Retention Time (min) Carisoprodol PCP Propoxyphene Sufentanil MAM Midazolam Normeperidine EDDP Methadone Lorazepam Clonazepam Norpropoxyphene Oxazepam Hydroxyalprazolam Nordiazepam Flunitrazepam Temazepam Alprazolam Diazepam

8 41 Pain Management Drugs from Urine in Under. Minutes Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Phenyl-Hexyl Achieve excellent resolution and separation of 41 pain management drugs using Kinetex core-shell Phenyl-Hexyl HPLC/UHPLC columns, a great choice for an alternative selectivity as compared to a C18. In addition to being a rapid separation, this method is also available as an imethod Application which allows AB SCIEX mass spec users to download the method and settings directly into their Cliquid software for instant method implementation. 4.6e6 4.2e6 3.8e6 3.4e6 3.e6 2.6e6 2.2e6 1.8e6 1.4e6 1.e6 6.e 2.e min App ID 2847 Column: Kinetex 2.6 µm Phenyl-Hexyl Dimensions: x 4.6 mm Part No.: B-449-E Guard: SecurityGuard ULTRA Phenyl-Hexyl Cartridges Part No.: AJ-8774 Mobile Phase: A: 1 mm Ammonium formate B:.1 % Formic Acid in Methanol Gradient: Time (min) % B 1 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 22 C Backpressure: 76.2 bar HPLC System: Agilent 126 Detection: MS/MS (AB SCIEX API ) 2.6 µm Skip the work and instantly run this method using the imethod Kit, a verified method that can be downloaded to your Cliquid software on your AB SCIEX mass spectrometer. imethod Kits contain MRM transitions, LC/MS/MS running conditions, sample preparation recommendations, and all of the consumables you will need to begin running samples. 8 Visit for more information

9 Analyte Q1 Q3 Retention Time (min) 6-MAM 1 (quantifier) MAM 2 (confirmation) Alprazolam Alprazolam Amphetamine Amphetamine Benzoylecgonine Benzoylecgonine Buprenorphine Buprenorphine Carisoprodol Carisoprodol Clonazepam Clonazepam Codeine Codeine Diazepam Diazepam EDDP EDDP Fentanyl Fentanyl Flunitrazepam Flunitrazepam Flurazepam Flurazepam Hydrocodone Hydrocodone Hydromorphone Hydromorphone Hydroxyalprazolam Hydroxyalprazolam Lorazepam Transition 1 for each analyte is used as the quantifier transition. Transition 2 for each analyte is used as the qualifier transition. Achieve Excellent Results, Every Time Recovery, Reproducibility, and Linearity of α-hydroxyalprazolam Concentration Precision Accuracy (ng/ml) (n = 3) Analyte Q1 Q3 Retention Time (min) Lorazepam MDA MDA MDEA MDEA MDMA MDMA Meperidine Meperidine Meprobamate Meprobamate Methadone Methadone Methamphetamine Methamphetamine Midazolam Midazolam Morphine Morphine Naloxone Naloxone Naltrexone Naltrexone Norbuprenorphine Norbuprenorphine Nordiazepam Nordiazepam Norfentanyl Norfentanyl Normeperidine Normeperidine Norpropoxyphene Norpropoxyphene Analyte area / IS Area Analyte Q1 Q3 Retention Time (min) Oxazepam Oxazepam Oxycodone Oxycodone Oxymorphone Oxymorphone PCP PCP Propoxyphene Propoxyphene Sufentanil Sufentanil Temazepam Temazepam Tramadol Tramadol MAM-d Amphetamine-d Benzoylecgonine-d Codeine-d Fentanyl-d Hydrocodone-d Hydromorphone-d Meperidine-d Methadone-d Methamphetamine-d Morphine-d Nordiazepam-d Normeperidine-d Norpropoxyphene-d PCP-d Recovery, 2. Reproducibility, and Linearity of Methamphetamine 1.. Concentration Precision Accuracy (ng/ml) (n = 3) 12 Analyte Conc./IS Conc Analyte area / IS Area Analyte area / IS Area Analyte Conc./IS Conc Analyte Conc./IS Conc

10 41 Pain Management Drugs from Urine in Under 4 Minutes Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell C18 Rapidly separate 41 pain management drugs, including critical isobars, using Kinetex core-shell C18 HPLC/UHPLC columns. 2.e6 1.9e6 1.8e6 1.7e6 1.6e6 1.e6 1.4e6 1.3e6 1.2e6 1.1e6 1.e6 9.e 8.e 7.e 6.e.e 4.e 3.e 2.e 1.e App ID 2689 Column: Dimensions: Part No.: Guard: Part No.: Kinetex 2.6 µm C18 x 3. mm B-4462-Y SecurityGuard ULTRA C18 Cartridges AJ-877 Mobile Phase: A: 1 mm Ammonium formate B:.1 % Formic Acid in Methanol Gradient: Time (min) % B 1 1 Flow Rate:. ml/min Injection Volume: 1 µl Column Temperature: 22 C Detection: MS/MS (AB SCIEX API 4 ) Analyte Q1 Q3 Oxymorphone Morphine Hydromorphone Amphetamine Methamphetamine Oxycodone MDA MDMA Codeine Naltrexone MDEA MAM Hydrocodone Benzoylecgonine Norfentanyl Tramadol Naloxone Normeperidine Meperidine Meprobamate PCP Norbuprenorphine EDDP Norpropoxyphene Clonazepam Flunitrazepam a-hydroxyalprazolam Carisoprodol Oxazepam Methadone Lorazepam Fentanyl Flurazepam Alprazolam Temazepam Propoxyphene Nordiazepam Diazepam Midazolam Sufentanil Buprenorphine Resolution of Critical Isobars Codeine/Hydrocodone 2.4e 2.e 1.e. 3.e 2.e 1.e. 1.e6 9.e 8.e 7.e 6.e.e 4.e 3.e 2.e 1.e min Morphine/Hydromorphone/Norhydrocodone min Oxymorphone/Dihydrocodeine/Noroxycodone Use our simplified SPE procedure on page 4 prior to HPLC/UHPLC analysis min 1

11 Benzodiazepines Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Accurately and reliably analyze benzodiazepines using Kinetex Biphenyl HPLC /UHPLC. 7.e 6.e.e 4.e 3.e 2.e 1.e min App ID 2219 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) Alprazolam 1 (quantifier) Alprazolam 2 (confirmation) Clonazepam Clonazepam Diazepam Diazepam Flunitrazepam Flunitrazepam Flurazepam Flurazepam Hydroxyalprazolam Hydroxyalprazolam Lorazepam Lorazepam Midazolam Midazolam Nordiazepam Nordiazepam Oxazepam Oxazepam Temazepam Temazepam

12 Opiates from Urine Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Achieve resolution of critical isobars such as Morphine / Hydromorphone and Codeine / Hydrocodone using Kinetex core-shell Biphenyl HPLC / UHPLC columns. 2.e6 1.e min App ID 2218 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) 6-MAM 1 (quantifier) MAM 2 (confirmation) Codeine Codeine EDDP EDDP Fentanyl Fentanyl Hydrocodone Hydrocodone Hydromorphone Hydromorphone Meperidine Meperidine Methadone Methadone Morphine Morphine Naloxone Naloxone Naltrexone Naltrexone Norfentanyl Norfentanyl Normeperidine Normeperidine Norpropoxyphene Norpropoxyphene Oxycodone Oxycodone Oxymorphone Oxymorphone Propoxyphene Propoxyphene Tramadol Tramadol

13 Miscellaneous Drugs of Abuse Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Separate various drugs of abuse including amphetamines, cocaine metabolites, and more. 1.2e6 1.e6 8.e 6.e 4.e 2.e min App ID 2211 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B Flow Rate:.6 ml/min Injection Volume: 1 µl Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analayte Q1 Q3 Retention Time (min) Amphetamine 1 (quantifier) Amphetamine 2 (confirmation) Benzoylecgonine Benzoylecgonine Carisoprodol Carisoprodol MDA MDA MDEA MDEA MDMA MDMA Meprobamate Meprobamate Methamphetamine Methamphetamine PCP PCP Sufentanil Sufentanil

14 Strata-X-Drug B SPE Finally, an SPE Sorbent for Drugs of Abuse Strata-X-Drug B is a polymeric strong cation-exchange SPE sorbent that is designed and quality controlled for basic drugs of abuse analysis. Save time and solvent; no conditioning or equilibrating required Achieve consistent, high recoveries; the sorbent is QC tested using drug probes from urine to ensure batch-to-batch reproducibility Reduce technician training requirements; use a single method to extract multiple drug classes Ordering Information Format Sorbent Mass Part Number Unit Price Tube 1 mg 8B-S128-AAK 1 ml (1/box) 3 mg 8B-S128-TAK 1 ml (1/box) 3 mg 8L-S128-TAK 1 ml (1/box) 3 mg 8B-S128-TBJ 3 ml (/box) 6 mg 8B-S128-UBJ 3 ml (/box) 6 mg 8B-S128-UCH 6 ml (3/box) 6 mg 8B-S128-UCL 6 ml (2/box) 96-Well Plate 1 mg 8E-S128-AGB 2 Plates/box 3 mg 8E-S128-TGB 2 Plates/box 6 mg 8E-S128-UGB 2 Plates/box Tab-less tube Accessories for Tubes Tabless Tube Holders Part No. Description Unit Price AH Well 1 ml Tabless Tube Holder for use with 96-well plate ea vacuum manifold (AH-89) AH-9 96-Well 1 ml Tabless Tube Holder for use with positive pressure manifolds ea Manifolds Part No. Description Unit Price AH-623 SPE 12-Position Vacuum Manifold Set, complete assembly ea AH-624 SPE 24-Position Vacuum Manifold Set, complete assembly ea Create a Customized SPE Method in Under 1 Minute Accessories for 96-Well Plates Manifold Part No. Description Unit Price AH Well Plate Manifold, Universal w/vacuum gauge ea Collection Plates (deep well, polypropylene) Part No. Description Unit Price AH Well Collection Plate, 3 µl/conical /pk AH Well Collection Plate, 1 ml/conical /pk AH Well Collectin Plate, 1 ml Round, 7 mm /pk AH Well Collection Plate, 2 ml Square/Round-Conical /pk AH Well Collection Plate, 2 ml Round/Round, 8 mm /pk Sealing Mats Part No. Description Unit Price AH-897 Sealing Mats, Pierceable, 96-Square Well, Silicone /pk AH-8631 Sealing Mats, Pierceable, 96-Round Well 7 mm, Silicone /pk AH-8633 Sealing Mats, Pierceable, 96-Round Well 8 mm, Silicone /pk AH-7362 Sealing Tape Pad 1/pk If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE. 14 Phenomenex l WEB:

15 Ordering Information Core-Shell Technology μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x x x 2.1 3/pk Biphenyl A-4627-AN B-4627-AN D-4627-AN AJ-929 C18 A-461-AN B-461-AN D-461-AN F-461-AN AJ-8782 Phenyl-Hexyl B-463-AN D-463-AN AJ-8788 for 2.1 mm ID μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases x 3. 1 x 3. 1 x 3. 3/pk Biphenyl B-4627-Y D-4627-Y F-4627-Y AJ-928 C18 B-461-Y D-461-Y F-461-Y AJ-877 Phenyl-Hexyl B-463-Y D-463-Y AJ-8781 for 3. mm ID μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases x x x x 4.6 3/pk Biphenyl B-4627-E D-4627-E F-4627-E G-4627-E AJ-927 C18 B-461-E D-461-E F-461-E G-461-E AJ-8768 Phenyl-Hexyl B-463-E D-463-E F-463-E G-463-E AJ-8774 for 4.6 mm ID 2.6 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x x x x 2.1 3/pk Biphenyl A-4622-AN B-4622-AN D-4622-AN F-4622-AN AJ-929 C18 A-4462-AN B-4462-AN C-4462-AN D-4462-AN F-4462-AN AJ-8782 Phenyl-Hexyl A-449-AN B-449-AN C-449-AN D-449-AN F-449-AN AJ-8788 for 2.1 mm ID 2.6 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 3. x 3. 7 x 3. 1 x 3. 1 x 3. 3/pk Biphenyl B-4622-Y D-4622-Y F-4622-Y AJ-928 C18 A-4462-Y B-4462-Y C-4462-Y D-4462-Y F-4462-Y AJ-877 Phenyl-Hexyl D-449-Y F-449-Y AJ-8781 for 3. mm ID 2.6 μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 4.6 x x x x 4.6 3/pk Biphenyl B-4622-E D-4622-E F-4622-E AJ-927 C18 A-4462-E B-4462-E C-4462-E D-4462-E F-4462-E AJ-8768 Phenyl-Hexyl B-449-E C-449-E D-449-E F-449-E AJ-8774 for 4.6 mm ID 1.7 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x x x 2.1 3/pk Biphenyl B-4628-AN D-4628-AN F-4628-AN AJ-929 C18 A-447-AN B-447-AN D-447-AN F-447-AN AJ-8782 Phenyl-Hexyl B-4-AN D-4-AN F-4-AN AJ-8788 for 2.1 mm ID 1.7 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases x 3. 1 x 3. 3/pk C18 B-447-Y D-447-Y AJ-877 for 3. mm ID 1.3 μm Minibore Columns (mm) Phases 3 x 2.1 x 2.1 C18 A-41-AN B-41-AN SecurityGuard ULTRA cartridges require holder, Part No.: AJ-9 If Phenomenex products in this brochure do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 4 days and keep the product for FREE. Phenomenex l WEB: 1

16 Access Clinical Research Guides, Applications, and More at Australia t: f: Austria t: f: Belgium t: (French) t: (Dutch) f: +31 () beinfo@phenomenex.com Canada t: (8) f: (31) info@phenomenex.com Denmark t: f: nordicinfo@phenomenex.com Finland t: f: nordicinfo@phenomenex.com France t: f: franceinfo@phenomenex.com Germany t: f: anfrage@phenomenex.com India t: f: indiainfo@phenomenex.com Ireland t: f: eireinfo@phenomenex.com Italy t: f: italiainfo@phenomenex.com Luxembourg t: +31 () f: +31 () nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: f: nlinfo@phenomenex.com New Zealand t: f: nzinfo@phenomenex.com Norway t: 81 2 f: nordicinfo@phenomenex.com Puerto Rico t: (8) 41-HPLC f: (31) info@phenomenex.com Sweden t: f: nordicinfo@phenomenex.com United Kingdom t: f: ukinfo@phenomenex.com United States t: (31) 212- f: (31) info@phenomenex.com All other countries: Corporate Office USA t: (31) 212- f: (31) info@phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com BR _I Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at Trademarks Kinetex is a registered trademark and Strata-X, Impact, MidBore, and SecurityGuard are trademarks of Phenomenex. Agilent is a registered trademark of Agilent Technologies, Inc. Cliquid is a registered trademark and API 4, API, and imethod are trademarks of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Disclaimers The products mentioned are not intended for clinical use. Because they are not intended for clinical use, no claim or representation is made or intended for their clinical use (including, but not limited to diagnostic, prognostic, therapeutic or blood banking). It is the user s responsibility to validate the performance of Phenomenex products for any particular use, since the performance characteristics are not established. Phenomenex products may be used in clinical diagnostic laboratory systems after the laboratory has validated their complete system as required by the Clinical Laboratory Improvements Amendments of 1988 (CLIA 88) regulation in the U.S. or equivalent in other countries. Strata-X is patented by Phenomenex. U.S. Patent No. 7,119, Phenomenex, Inc. All rights reserved.