Element B1 / 5 Toxicology and Testing
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1 / 5 Toxicology and Testing
2 Toxicology Study of nature and effects of toxic substances, their detection and treatment
3 Toxicology Toxin Substance, which when absorbed into body, has ability to cause harm by upsetting metabolic processes No indication to level of harm Not poisons severe harm or death All substances toxic in right conditions
4 Epidemiology Study of patterns of health and ill health in relation to their associated factors, at the population level Focussed on groups rather than ind. Historical perspective analyse patterns
5 Factors Affecting Toxicity Duration of exposure Short term exposure can result in different effects than long term Interaction with other chemicals Additive, synergistic, potentiation, antagonistic Species Sex Genetics Age Health
6 Toxicology in Occ. Health Setting exposure limits Classification and labelling Preparation of SDS ID thresholds ID persons at risk Development of biological monitoring methods Predicting effects of new chemicals R/A s occupationally exposed and population at large
7 Human Epidemiological Investigations Types of study Morbidity / mortality stats Morbidity data incidence of disease on a community or pop. Mortality data extracted from death certs cause of death and occupation recorded Cross sectional survey Measures exposure to risk and evidence of disease in groups Case control studies Investigate frequency of members of a group who have particular problems or diseases Compared to control group
8 Human Epidemiological Investigations Types of study Retrospective cohort studies Look back in time Result / ill health is unknown Try to find cause Cohort = no. of individuals who have the problem and who have been selected for the study Prospective cohort studies Look forward in time Takes long time to conduct Each individual studied over time
9 Vertebrate Animal Testing Ltd value unless life form has physiology close to humans i.e. primates Acute tests Less than a month Sub-chronic 1 to 3 months Chronic > 3 months
10 Vertebrate Animal Testing LD50 Dose required to kill 50% of the test population Replaced by fixed dose procedure Observation of clear signs of toxicity at one of a series of fixed dose levels Only moderately toxic doses used Lethal doses avoided (as well as marked pain and distress)
11 Vertebrate Animal Testing Toxic if inhaled, ingested, penetrate skin Can cause serious acute or chronic health risks, even death Toxicity Defined as ability to cause above in a population Highly toxic Can cause death, even at low doses
12 Vertebrate Animal Testing Dose Animal studies Amount administered Humans Uptake of chemical from airborne, biological monitoring, diet Dose effect Variation in degree of effect with changing dose
13 Vertebrate Animal Testing Dose response Toxins have different effects on organisms Factors Min level at which an effect is detectable Sensitivity of the organism to small increases in dose Level at which death / harm occurs Damage varies with dose
14 Vertebrate Animal Testing Dose response Proportion of population that will demonstrate an effect at a given dose Used to derive several values LD50 LC50 etc NOAEL No observed adverse effect level Highest exposure level where no effects Some chemicals (carcinogens) No NOAEL
15 Alternative to Animal Testing AMES tests Biological Assay to assess mutagenic potential Uses bacteria (salmonella) mixed with rats liver enzyme Limited not always possible to extrapolate to man Biological organisms do not breathe, see, digest etc.
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