Kit #9 Normal Mitosis

Transcription

1 Kit #9 Normal Mitosis 9-MSDS

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3 1. IDENTIFICATION 1.1 Product Identifiers Product Name: Alternative names: Product Number: Solution A Denatured alcohol and hydrochloric acid solution. 9-1EA 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses: For laboratory and educational use only 1.3 Details of the supplier of the safety data sheet Company: LAB-AIDS, Inc, 17 Colt Ct., Ronkonkoma, NY 11779, USA Telephone: Fax: Emergency telephone number Emergency number: CHEMTREC HAZARDS IDENTIFICATION 2.1 Classification of the substance or mixture GHS classification Flam. Liq. (Category 2), H225 Skin Corr. (Category 1B), H314 Eye Corr. (Category 1), H Label elements, including precautionary statements Signal word: Danger Pictogram: Hazards statements: H225 - Highly flammable liquid and vapor, H314 Causes severe skin burns and eye damage. SAFETY DATA SHEET Version: SDS_ Revision Date: March 27, 2015 Precautionary statements: P210 - Keep away from heat, hot surfaces, open flames, sparks, P280 - Wear protective gloves, eye protection 2.3 Hazards not otherwise classified: none 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substance: Not applicable 3.2 Mixture Chemical Name Product identifier % GHS-US classification Ethyl alcohol, denatured Hydrochloric acid CAS# CAS# Flam. Liq. 2, H225; STOT SE 3; H336 Acute Tox. 4 (Oral), H302; Skin Corr. 1B H314 ; Eye Dam. 1, H318; STOT SE Chemicals where a trade secret is claimed: Concentration of the components. 4. FIRST AID MEASURE 4.1 Description of the first aid measure: INGESTION: Never give anything by mouth to unconscious person. Rinse mouth and get conscious person drink a glass of milk or water. Do NOT induce vomiting unless directed to do so by medical personnel. Get immediate medical attention. INHALATION: Remove to fresh air. Get medical attention if necessary. EYE CONTACT: Wash immediately with plenty of water, and continue washing for at least 15min., occasionally lifting upper and lower eyelids. Seek an immediate medical attention. SKIN CONTACT: Flush thoroughly with mild soap and water. Remove contaminated clothing. Get medical attention if burns or irritation develop. 4.2 Most important symptoms and effects, both acute and delayed: Refer to section Indication of any immediate medical attention and special treatment needed: No additional information available. 5. FIREFIGHTING MEASURES 5.1 Extinguishing media: Flammable liquid. Suitable extinguishing media: Use TriClass, dry chemical extinguisher. 5.2 Special hazard arising from the substance or mixture: Flammable liquid. Vapor spreads at floor level and may travel to the ignition source. 5.3 Advice for firefighters: Use self-contained breathing apparatus and protective clothing. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: Wear laboratory grade gloves, eye protection and a lab coat. 6.2 Emergency procedures: Restrict unprotected personnel from the area. 6.3 Methods and material used for containment and cleanup procedure: Contain the spill with an inert absorbent material and deposit in a sealed container. Ventilate and wash spill area with soap and water. 7. HANDLING AND STORAGE 7.1 Precaution for safe handling: Read label on container before using. Keep away from heat, sparks and flame. Vapor forms from this product and may travel, or be moved by air currents and ignited by pilot lights, static discharges, flames, sparks, heaters or other ignition sources. Do not wear contact lenses when working with chemicals. For laboratory use only. Not for drug, food or household use. Use only under adult supervision. Avoid breathing vapor. Use hood or with adequate ventilation. Wash hands thoroughly after handling. 7.2 Storage: Flammable cabinet. Keep container in cool, well-ventilated area. 7.3 incompatibility: Refer to section 10. 1

4 8. EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1 Control parameters: ACGIH: TWA: 2ppm (hydrochloric acid); ACGIH: TWA: 1000ppm (ethanol) 8.2 Exposure controls: Avoid contact with eyes, skin, and clothing. Wear chemical splash goggles, chemical-resistant gloves, and chemical-resistant apron. Use ventilation to keep airborne concentrations below exposure limits. Respiratory protection: Non should be needed if normal laboratory handling at room temperature. 9. PHYSICAL AND CHEMICAL PROPERTIES Physical state: Liquid. Appearance: Transparent. Odor: Strong alcohol odor ph: Acidic Vapor Pressure ( mm Hg): Not available Vapor Density: Not available Evaporation Rate: Not available Viscosity: Flash point: 16 C (61 F) ethanol Autoignition: 365 C (689 F) 10. STABILITY AND REACTIVITY Boiling point: C ( F ) ethanol Melting point: -114 C ( -173 F ) ethanol Freezing point: Not available Decomposition temp: Not available Solubility: Miscible in water Specific gravity (H₂O = 1): Not available Percent volatile (%): Not available Molecular formula: Mixture Molecular weight: Mixture Chemical Stability: Stable Conditions to Avoid: Open flames, sparks, high temperatures and incompatible materials. Incompatibilities: Strong oxidizing agents, acid chlorides, metals, peroxides, ammonia, organic materials. Hazardous decomposition: Not available Hazardous polymerization: Will not occur. 11. TOXICOLOGICAL INFORMATION Acute effects: Eyes: Corrosive to eyes. Splashes may cause severe burns and permanent eye damage. Skin: Corrosive to skin. Can cause redness, pain and severe skin burns if not washed off immediately. Inhalation: Vapors are irritating to mucous membrane and eyes. Inhalation of vapors can cause coughing, choking. Inflammation of the nose, throat, and upper respiratory tract. Ingestion: Swallowing hydrochloric acid can cause immediate pain and burns of the mouth, throat, esophagus and gastrointestinal tract. Ingested doses of alcohol are toxic and harmful when swallowed. Alcohol will produce nausea, dizziness and headache. Toxicological data: ORAL LD₅₀Ethanol: 7060mg/kg [Rat]. 3450mg/kg [Mouse] VAPOR LC₅₀Ethanol: 20000ppm 8h [Rat] mg/m³ 4h [Mouse] Acute oral toxicity ORAL LD₅₀: 900mg/kg [Rabbit], as hydrochloric acid Acute vapor toxicity IHL-LC₅₀: 3124ppm [Rat]/1h, as hydrochloric acid DERMAL LD₅₀: not available Carcinogenicity: California prop 65: Not classified 12. ECOLOGICAL INFORMATION Not available. 13. DISPOSAL CONSIDERATION Disposal of in accordance with all local, state, and federal regulations, or contact with a licensed chemical disposal agency. 14. TRANSPORT INFORMATION UN number: 2924 Shipping name: Flammable liquid, corrosive, n.o.s. Hazard Class: 3, 8 Packing group: PG II Exceptions: Lty Qty 1L 15. REGULATORY INFORMATION TSCA-listed, EINECS-listed ( ) as alcohol. TSCA-listed, EINECS-listed ( )as hydrochloric acid. WHMIS (Canada): CLASS E: Corrosive liquid. DSCL (EEC) R34-Causes burns. 16. OTHER INFORMATION Disclaimer: The Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. LAB-AIDS makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation and verification. The data should not be confused with local, state, federal regulations, or insurance mandates, and CONSTITUTE NO WARRANTY. Any use of these data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond LAB-AIDS, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S). Version: SDS_ Revision Date: March 27,

5 1. IDENTIFICATION 1.1 Product Identifiers Product Name: Alternative names: Product Number: Solution B Denatured alcohol and chloroform acidic solution. 9-2EA 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses: For laboratory and educational use only 1.3 Details of the supplier of the safety data sheet Company: LAB-AIDS, Inc, 17 Colt Ct., Ronkonkoma, NY 11779, USA Telephone: Fax: Emergency telephone number Emergency number: CHEMTREC HAZARDS IDENTIFICATION 2.1 Classification of the substance or mixture GHS classification Flam. Liq. (Category 2), H225 Skin Corr. (Category 1B), H314 Eye Corr. (Category 1), H Label elements, including precautionary statements Signal word: Danger Pictogram: Hazards statements: H225 - Highly flammable liquid and vapor, H314 Causes severe skin burns and eye damage. SAFETY DATA SHEET Version: SDS_ Revision Date: March 27, 2015 Precautionary statements: P210 - Keep away from heat, hot surfaces, open flames, sparks, P280 - Wear protective gloves, eye protection 2.3 Hazards not otherwise classified: none 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substance: Not applicable 3.2 Mixture Chemical Name Product identifier % GHS-US classification Ethyl alcohol, denatured Chloroform Acetic acid CAS# CAS# CAS# Flam. Liq. 2, H225; STOT SE 3; H336 Acute Tox. 4 (Oral), H302; Skin Irrit. 2A H315 ; Carc. 2. H351; Repr. 2, H361; Flam. Liq. 3, H226; Skin Corr. 1A, H314, Eye Dam. H Chemicals where a trade secret is claimed: Concentration of the components. 4. FIRST AID MEASURE 4.1 Description of the first aid measure: INGESTION: Never give anything by mouth to unconscious person. Rinse mouth and get conscious person drink a glass of milk or water. Do NOT induce vomiting unless directed to do so by medical personnel. Get immediate medical attention. INHALATION: Remove to fresh air. Get medical attention if necessary. EYE CONTACT: Wash immediately with plenty of water, and continue washing for at least 15min., occasionally lifting upper and lower eyelids. Seek an immediate medical attention. SKIN CONTACT: Flush thoroughly with mild soap and water. Remove contaminated clothing. Get medical attention if burns or irritation develop. 4.2 Most important symptoms and effects, both acute and delayed: Refer to section Indication of any immediate medical attention and special treatment needed: No additional information available. 5. FIREFIGHTING MEASURES 5.1 Extinguishing media: Flammable liquid. Suitable extinguishing media: Use TriClass, dry chemical extinguisher. 5.2 Special hazard arising from the substance or mixture: Flammable liquid. Vapor spreads at floor level and may travel to the ignition source. 5.3 Advice for firefighters: Use self-contained breathing apparatus and protective clothing. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: Wear laboratory grade gloves, eye protection and a lab coat. 6.2 Emergency procedures: Restrict unprotected personnel from the area. 6.3 Methods and material used for containment and cleanup procedure: Contain the spill with an inert absorbent material and deposit in a sealed container. Ventilate and wash spill area with soap and water. 7. HANDLING AND STORAGE 7.1 Precaution for safe handling: Read label on container before using. Keep away from heat, sparks and flame. Vapor forms from this product and may travel, or be moved by air currents and ignited by pilot lights, static discharges, flames, sparks, heaters or other ignition sources. Do not wear contact lenses when working with chemicals. For laboratory use only. Not for drug, food or household use. Use only under adult supervision. Avoid breathing vapor. Use hood or with adequate ventilation. Wash hands thoroughly after handling. 7.2 Storage: Flammable cabinet. Keep container in cool, well-ventilated area. 7.3 incompatibility: Refer to section 10. 1

6 8. EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1 Control parameters: ACGIH: TWA: 25g/m³ (acetic acid); ACGIH: TWA: 1000ppm (ethanol); ACGIH: TWA: 10ppm (chloroform). 8.2 Exposure controls: Avoid contact with eyes, skin, and clothing. Wear chemical splash goggles, chemical-resistant gloves, and chemical-resistant apron. Use ventilation to keep airborne concentrations below exposure limits. Respiratory protection: Non should be needed if normal laboratory handling at room temperature. 9. PHYSICAL AND CHEMICAL PROPERTIES Physical state: Liquid. Appearance: Transparent. Odor: Strong alcohol odor ph: Acidic Vapor Pressure ( mm Hg): Not available Vapor Density: Not available Evaporation Rate: Not available Viscosity: Flash point: 16 C (61 F) ethanol Autoignition: 365 C (689 F) 10. STABILITY AND REACTIVITY Boiling point: C ( F ) ethanol Melting point: -114 C ( -173 F ) ethanol Freezing point: Not available Decomposition temp: Not available Solubility: Miscible in water Specific gravity (H₂O = 1): Not available Percent volatile (%): Not available Molecular formula: Mixture Molecular weight: Mixture Chemical Stability: Stable Conditions to Avoid: Open flames, sparks, high temperatures and incompatible materials. Incompatibilities: Strong oxidizing agents, acid chlorides, metals, peroxides, ammonia, organic materials. Hazardous decomposition: Not available Hazardous polymerization: Will not occur. 11. TOXICOLOGICAL INFORMATION Acute effects: Eyes: Corrosive to eyes. Splashes may cause severe burns and permanent eye damage. Skin: Corrosive to skin. Can cause redness, pain and severe skin burns if not washed off immediately. Inhalation: Vapors are irritating to mucous membrane and eyes. Inhalation of vapors can cause coughing, choking. Inflammation of the nose, throat, and upper respiratory tract. Ingestion: Ingestion: Toxic. Harmful if swallowed. Swallowing causes gastrointestinal track irritation with possible burns associated with pain of the mouth, throat. Ingested doses of alcohol will produce nausea, dizziness and headache. Ingestion of chloroform may affect liver, kidneys. Prolonged or repeated ingestion may damage these organs Toxicological data: ORAL LD₅₀Ethanol: 7060mg/kg [Rat]. 3450mg/kg [Mouse] VAPOR LC₅₀Ethanol: 20000ppm 8h [Rat] mg/m³ 4h [Mouse] Acute oral toxicity ORAL LD₅₀: 695mg/kg [Rat], as chloroform Acute vapor toxicity IHL-LC₅₀: mg/m³ [Rat]/4h, as chloroform DERMAL LD₅₀: >20000mg/kg [Rabbit], as chloroform Acute oral toxicity LD₅₀: 3310mg/kg [Rat], as acidic acid Acute dermal toxicity LD₅₀ 1060mg/kg [Rabbit], as acidic acid Acute vapor toxicity LC₅₀: 5620ppm 1h [Mouse], as acidic acid Carcinogenicity: California prop 65: This product contains the following ingredients for which the State of California has found to cause cancer, birth defects, or other reproductive harm, which would require a warning under the statue: Chloroform. 12. ECOLOGICAL INFORMATION Ecotoxicity in water (LC50): 43.8mg/l 96h [Trout]. 13. DISPOSAL CONSIDERATION Disposal of in accordance with all local, state, and federal regulations, or contact with a licensed chemical disposal agency. 14. TRANSPORT INFORMATION UN number: 3286 Shipping name: Flammable liquid, toxic, corrosive, n.o.s. Hazard Class: 3, 6.1, 8 Packing group: PG II Exceptions: Lty Qty 1L 15. REGULATORY INFORMATION TSCA-listed, EINECS-listed ( ) as alcohol. DSCL (EEC) R22-Harmful if swallowed. R40 Limited evidence of carcinogenic effect as chloroform. R48/20/22 Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed as chloroform. 16. OTHER INFORMATION Disclaimer: The Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. LAB-AIDS makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation and verification. The data should not be confused with local, state, federal regulations, or insurance mandates, and CONSTITUTE NO WARRANTY. Any use of these data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond LAB-AIDS, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S). Version: SDS_ Revision Date: March 27,

7 1. IDENTIFICATION 1.1 Product Identifiers Product Name: Alternative names: Product Number: Aceto-Orcein Stain Ethanoic acid mixture. 9-3EA 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses: For laboratory and educational use only 1.3 Details of the supplier of the safety data sheet Company: LAB-AIDS, Inc, 17 Colt Ct., Ronkonkoma, NY 11779, USA Telephone: Fax: Emergency telephone number Emergency number: CHEMTREC HAZARDS IDENTIFICATION 2.1 Classification of the substance or mixture GHS classification Flam. Liq. (Category 3), H226 Skin Corr. (Category 1B), H314 Eye Corr. (Category 1), H Label elements, including precautionary statements Signal word: Danger Pictogram: Hazards statements: H226 - Flammable liquid and vapor, H314 Causes severe skin burns and eye damage. SAFETY DATA SHEET Version: SDS_ Revision Date: March 27, 2015 Precautionary statements: P210 - Keep away from heat, hot surfaces, open flames, sparks, P280 - Wear protective gloves, eye protection 2.3 Hazards not otherwise classified: none 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substance: Not applicable 3.2 Mixture Water Acetic acid Orcein Chemical Name Product identifier % GHS-US classification CAS# CAS# CAS# Not classified Flam. Liq. 3, H226; Skin Corr. 1A, H314, Eye Dam. H318 Acute Tox. 4, H Chemicals where a trade secret is claimed: Concentration of the components. 4. FIRST AID MEASURE 4.1 Description of the first aid measure: INGESTION: Never give anything by mouth to unconscious person. Rinse mouth and get conscious person drink a glass of milk or water. Do NOT induce vomiting unless directed to do so by medical personnel. Get immediate medical attention. INHALATION: Remove to fresh air. Get medical attention if necessary. EYE CONTACT: Wash immediately with plenty of water, and continue washing for at least 15min., occasionally lifting upper and lower eyelids. Seek an immediate medical attention. SKIN CONTACT: Flush thoroughly with mild soap and water. Remove contaminated clothing. Get medical attention if burns or irritation develop. 4.2 Most important symptoms and effects, both acute and delayed: Refer to section Indication of any immediate medical attention and special treatment needed: No additional information available. 5. FIREFIGHTING MEASURES 5.1 Extinguishing media: Flammable liquid. Suitable extinguishing media: Use TriClass, dry chemical extinguisher. 5.2 Special hazard arising from the substance or mixture: Flammable liquid. Vapor spreads at floor level and may travel to the ignition source. 5.3 Advice for firefighters: Use self-contained breathing apparatus and protective clothing. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: Wear laboratory grade gloves, eye protection and a lab coat. 6.2 Emergency procedures: Restrict unprotected personnel from the area. 6.3 Methods and material used for containment and cleanup procedure: Contain the spill with an inert absorbent material and deposit in a sealed container. Ventilate and wash spill area with soap and water. 7. HANDLING AND STORAGE 7.1 Precaution for safe handling: Read label on container before using. Keep away from heat, sparks and flame. Vapor forms from this product and may travel, or be moved by air currents and ignited by pilot lights, static discharges, flames, sparks, heaters or other ignition sources. Do not wear contact lenses when working with chemicals. For laboratory use only. Not for drug, food or household use. Use only under adult supervision. Avoid breathing vapor. Use hood or with adequate ventilation. Wash hands thoroughly after handling. 7.2 Storage: Flammable cabinet. Keep container in cool, well-ventilated area. 7.3 incompatibility: Refer to section 10. 1

8 8. EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1 Control parameters: ACGIH: TWA: 25g/m³ (acetic acid). 8.2 Exposure controls: Avoid contact with eyes, skin, and clothing. Wear chemical splash goggles, chemical-resistant gloves, and chemical-resistant apron. Use ventilation to keep airborne concentrations below exposure limits. Respiratory protection: Non should be needed if normal laboratory handling at room temperature. 9. PHYSICAL AND CHEMICAL PROPERTIES Physical state: Liquid. Appearance: Red. Odor: Strong acetic odor ph: Acidic Vapor Pressure ( mm Hg): Not available Vapor Density: Not available Evaporation Rate: Not available Viscosity: Flash point: 40.3 C (104 F) acetic acid Autoignition: 485 C (905 F) acetic acid 10. STABILITY AND REACTIVITY Boiling point: 117 C (243 F ) acetic acid Melting point: 16.2 C ( 61.2 F ) acetic acid Freezing point: Not available Decomposition temp: Not available Solubility: Miscible in water Specific gravity (H₂O = 1): Not available Percent volatile (%): Not available Molecular formula: Mixture Molecular weight: Mixture Chemical Stability: Stable Conditions to Avoid: Open flames, sparks, high temperatures and incompatible materials. Incompatibilities: Strong oxidizing agents, chromic and nitric acid, carbonates, metals, hydroxides, amines, organic materials. Hazardous decomposition: Not available Hazardous polymerization: Will not occur. 11. TOXICOLOGICAL INFORMATION Acute effects: Eye: Causes eye irritation, redness and pain, May cause burns, blurred vision, conjunctivitis, corneal destruction and permanent injury. Stains tissue. Skin: Causes skin irritation. May cause blistering, tissue burns and damage. Stains tissue. Inhalation: Causes respiratory track irritation. Affects the sense organs. Corrosive to mucous membrane. Severe exposure may result in lung tissue damage. Ingestion: Causes gastrointestinal track irritation with possible burns associated with burning and pain of the mouth, throat, nausea, diarrhea, abdominal pain. May also affect blood, metabolism, liver and the urinary system. Toxicological data: Acute oral toxicity LD₅₀: 3310mg/kg [Rat], as acidic acid Acute dermal toxicity LD₅₀ 1060mg/kg [Rabbit], as acidic acid Acute vapor toxicity LC₅₀: 5620ppm 1h [Mouse], as acidic acid Carcinogenicity: California prop 65: Not classified. 12. ECOLOGICAL INFORMATION Not available. 13. DISPOSAL CONSIDERATION Disposal of in accordance with all local, state, and federal regulations, or contact with a licensed chemical disposal agency. 14. TRANSPORT INFORMATION UN number: 2790 Shipping name: Acetic acid solution Hazard Class: 8 Packing group: III Exceptions: Ltd Qty. 4L 15. REGULATORY INFORMATION TSCA-listed. DSCL (EEC) R36/37/38 Irritating to eyes, respiratory system and skin. R35-Causes severe burns. 16. OTHER INFORMATION Disclaimer: The Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. LAB-AIDS makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation and verification. The data should not be confused with local, state, federal regulations, or insurance mandates, and CONSTITUTE NO WARRANTY. Any use of these data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond LAB-AIDS, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S). Version: SDS_ Revision Date: March 27,

9 1. IDENTIFICATION 1.1 Product Identifiers Product Name: Alternative names: Product Number: Root Tips 70% Isopropyl alcohol 9-4EA 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses: For laboratory and educational use only 1.3 Details of the supplier of the safety data sheet Company: LAB-AIDS, Inc, 17 Colt Ct., Ronkonkoma, NY 11779, USA Telephone: Fax: Emergency telephone number Emergency number: CHEMTREC HAZARDS IDENTIFICATION 2.1 Classification of the substance or mixture GHS classification Flam. Liq. (Category 2), H225 Eye irritant (Category 2A), H319 SAFETY DATA SHEET Version: SDS_ Revision Date: March 27, Label elements, including precautionary statements Signal word: Danger Pictogram: Hazards statements: H225 - Highly flammable liquid and vapor, H319 - Causes serious eye irritation. Precautionary statements: P210 - Keep away from heat, hot surfaces, open flames, sparks, P280 - Wear protective gloves, eye protection 2.3 Hazards not otherwise classified: none 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substance: Not applicable 3.2 Mixture Water Isopropanol Chemical Name Product identifier % GHS-US classification CAS# CAS# % 70% Not classified Flam. Liq. 2, H225; Eye Irrit. 2A ; STOT SE 3, H Chemicals where a trade secret is claimed: None 4. FIRST AID MEASURE 4.1 Description of the first aid measure: INGESTION: Never give anything by mouth to unconscious person. Rinse mouth and get conscious person drink a glass of milk or water. Do NOT induce vomiting unless directed to do so by medical personnel. Get immediate medical attention. INHALATION: Remove to fresh air. Get medical attention if necessary. EYE CONTACT: Wash immediately with plenty of water, and continue washing for at least 15min., occasionally lifting upper and lower eyelids. Seek medical attention if necessary. SKIN CONTACT: Flush thoroughly with mild soap and water. Remove contaminated clothing. Get medical attention if irritation develop. 4.2 Most important symptoms and effects, both acute and delayed: Exposure to high concentrations may cause coughing and central nervous system depression. Dizziness. Headache. 5. FIREFIGHTING MEASURES 5.1 Extinguishing media Suitable extinguishing media: Use TriClass, dry chemical extinguisher. 5.2 Special hazard arising from the substance or mixture: Vapor spreads at floor level. 5.3 Advice for firefighters: Use self-contained breathing apparatus and protective clothing. 6. ACCIDENTAL RELEASE MEASURES 6.1 Personal precautions: Wear laboratory grade gloves, eye protection and a lab coat. 6.2 Emergency procedures: Restrict unprotected personnel from the area. 6.3 Methods and material used for containment and cleanup procedure: For small spill use paper towel. 7. HANDLING AND STORAGE 7.1 Precaution for safe handling: Read label on container before using. Do not wear contact lenses when working with chemicals. For laboratory use only. Not for drug, food or household use. Use only under adult supervision. Use hood or with adequate ventilation. Avoid breathing vapor. Wash hands thoroughly after handling. 7.2 Storage: Keep container tightly closed in cool, well ventilated area. Keep away from open flames, sparks and heat. 7.3 incompatibility: Refer to section 10. 1

10 8. EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1 Control parameters: ACGIH: STEL: 980g/m³; USA OSHA: TWA: 490g/m³ 8.2 Exposure controls: Avoid contact with eyes, skin, and clothing. Wear chemical splash goggles, chemical-resistant gloves, and chemical-resistant apron. Use ventilation to keep airborne concentrations below exposure limits. Respiratory protection: Non should be needed if normal laboratory handling at room temperature. Use self-contained breathing apparatus in high vapor concentrations. 9. PHYSICAL AND CHEMICAL PROPERTIES Physical state: Liquid. Appearance: Transparent. Odor: Alcohol ph: Not available Vapor Pressure ( mm Hg): 32.4 at 20 C/68 F (Isopropyl alcohol) Vapor Density: the Not available Evaporation Rate: Not available Viscosity: Flash point: 12 C/53.6 F (isopropyl alcohol) Autoignition: 399 C/750.2 F (Isopropyl alcohol) 10. STABILITY AND REACTIVITY Chemical Stability: Stable Conditions to Avoid: High temperatures, sparks open flames and incompatible materials. Incompatibilities: Alkali metals, metals, organic materials, strong oxidizing agents, amines. Hazardous decomposition: not available. Hazardous polymerization: Will not occur. Boiling point: 82 C/180 F (isopropyl alcohol) Melting point: C/ F (isopropyl alcohol) Freezing point: Not available Decomposition temp: Not available Solubility: Miscible in water and alcohol Specific gravity (H₂O = 1): g/cm³ Percent volatile (%): Not available Molecular formula: Mixture Molecular weight: Mixture 11. TOXICOLOGICAL INFORMATION Acute effects: Eyes: Material is irritating to eyes. Skin: May cause mild skin irritation. Inhalation: Inhalation of large quantities might produce nausea, dizziness and headache. Ingestion: Ingestion may affect reversible liver effects and Central System Depression similar to inhalation. Toxicological data: ORAL LD₅₀Isopropyl alcohol: 5045mg/kg [Rat]. 3600mg/kg [Mouse] mg/kg [Rabbit] DERMAL LD₅₀Isopropyl alcohol: mg/kg 4h [Rabbit]. Carcinogenicity: California prop 65: Not classified 12. ECOLOGICAL INFORMATION Data not available. 13. DISPOSAL CONSIDERATION Disposal of in accordance with all local, state, and federal regulations. 14. TRANSPORT INFORMATION UN number: 1219 Shipping name: Isopropanol solution Hazard Class: 3 Packing group: PG II Exceptions: Lty Qty 1L 15. REGULATORY INFORMATION TSCA-listed, EINECS-listed ( ) isopropyl alcohol. R11- Highly flammable, R36 Irritating to eyes, R67 Vapors may cause drowsiness and dizziness. 16. OTHER INFORMATION Disclaimer: The Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. LAB-AIDS makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation and verification. The data should not be confused with local, state, federal regulations, or insurance mandates, and CONSTITUTE NO WARRANTY. Any use of these data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond LAB-AIDS, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S). Version: SDS_ Revision Date: March 27,