Effects of cooling on skeletal muscle recovery after exercise Arthur J. Cheng, Ph.D. Researcher Department of Physiology and Pharmacology Cellular Muscle Function Laboratory
Recovery from Fatigue - Soccer Arthur J. Cheng 20/02/2018 2
Recovery Cold Water Immersion (CWI) Arthur J. Cheng 20/02/2018 3
Whole Muscle to Single Fibre Skeletal muscle fibre (cell) Arthur J. Cheng 20/02/2018 4
Segment of a Single Fibre Arthur J. Cheng 20/02/2018 5
Excitation-Contraction Coupling sarcolemma myoplasm DHPR RyR1 Ca 2+ Ca 2+ Ca 2+ Ca 2+ SERCA Ca 2+ myofilaments
Excitation-Contraction Coupling sarcolemma myoplasm DHPR RyR1 SERCA Ca2+ Ca2+ Ca 2+ Ca 2+ Ca 2+ myofilaments
Intact single muscle fibre force transducer Micropipette Fluorescent Ca 2+ indicator 120 x tendon single fibre (1 mm) tendon Cheng and Westerblad, 2017 Nat Protoc Arthur J. Cheng 20/02/2018 8
Force-[Ca 2+ ] i Relationship 70 Hz 100 Hz 120 Hz Force 50 Hz 30 Hz 10 Hz [Ca 2+ ] i Arthur J. Cheng 20/02/2018 9
Fatigue in human muscle fibres (Unpublished data) Arthur J. Cheng 20/02/2018 10
Glycogen Depletion During Fatigue Before fatigue After fatigue Neilsen, Cheng et al., 2014 J Physiol Neilsen, Cheng et al., 2014 J Physiol Arthur J. Cheng 20/02/2018 11
SR Ca 2+ Release and Glycogen Nielsen et al., 2009 J Physiol Arthur J. Cheng 20/02/2018 12
Glycogen Resynthesis is Slow After competitive soccer match Krustrup et al., 2011 Eur J Appl Physiol Arthur J. Cheng 20/02/2018 13
Recovery Cold Water Immersion Michael Phelps and Ryan Lochte, Rio Olympics 2016 Arthur J. Cheng 20/02/2018 14
Recovery Cold Water Immersion Post-exercise recovery of contractile function and endurance in humans and mice is accelerated by heating and slowed by cooling skeletal muscle. Cheng, A.J., Willis, S.J., Zinner, C., Chaillou, T., Ivarsson, N., Ortenblad, N., Lanner, J.T., Holmberg, H.C., Westerblad, H. (2017) The Journal of Physiology 595(24):7413-7426 Arthur J. Cheng 20/02/2018 15
Effects of Temperature on Recovery From Fatigue Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 16
Swedish Winter Sports Research Institute Cheng et al., 2017 J Physiol Arthur J. Cheng 17
Cooling Depresses Power Output Fatigue Test 1 2 h recovery Fatigue Test 2 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 18
Fatigue in single muscle fibres 28 um Cheng and Westerblad, 2017 Nat Protoc Arthur J. Cheng 20/02/2018 19
Cooling Depresses SR Ca 2+ Release Fatigue Induction 2 h recovery 30 Hz 36 C 31 C 26 C 16 C 31 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 20
Cooling Slows Force Recovery Submaximal force (30 Hz) Muscle Glycogen 26 36 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 21
Bottomline: CWI and Recovery Post-exercise timeline 0-2 h: Cooling blunts recovery of strength, endurance, and glycogen resynthesis (Cheng et al., 2017 J Physiol) 0-48 h: 10 min CWI does not reduce muscle inflammation after one session of resistance exercise (Peake et al., 2017 J Physiol) 12 weeks strength training: 10 min CWI after every training session blunts increases in muscle mass and strength (Roberts et al., 2015 J Physiol) Arthur J. Cheng 20/02/2018 22
Bottomline: Arthur J. Cheng 20/02/2018 23
Acknowledgements Karolinska Institutet Håkan Westerblad Niklas Ivarsson Johanna Lanner Funding Sources Mid Sweden University Hans Christer Holmberg Sarah Willis Christoph Zinner Örebro University Thomas Chaillou University of Southern Denmark Niels Ørtenblad
Thank you! Website: https://www.researchgate.net/profile/arthur_cheng2 Email: arthur.cheng@ki.se Arthur J. Cheng 20/02/2018 25
CWI and long-term muscle adaptation 12-week lower body strength training: Twice per week, 8-12 RM (leg press, knee extension, knee flexion, lunges, plyometrics) 1. Recovery: 10min 37 W cycling (ACT) 2. Recovery: 10 min at 10 C (CWI) Roberts et al., 2015 J Physiol Arthur J. Cheng 20/02/2018 26
What about CWI for inflammation? Exercise: 18 sets x 8-12 reps (squats, knee extension, lunges) 1. Recovery: 10 min 37 W cycling (ACT) 2. Recovery: 10 min at 10 C (CWI) Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 27
What about CWI for inflammation? mrna expression Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 28
What about CWI for inflammation? mrna expression Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 29
Cooling Decreases Endurance After 1h recovery at 26 After 1h recovery at 36 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/2018 30
Fatigue and glycogen depletion 60 min submaximal cycling 1 min supramaximal cycling Bergstrom and Hultman,1967 Gollnick PD et al., 1973 Arthur J. Cheng 20/02/2018 31
Fatigue in Mouse Single Fibre Force Arthur J. Cheng 20/02/2018 32
Recovery Cold Water Immersion Arthur J. Cheng 20/02/2018 33
Cooling Slows Glycogen Resynthesis 26 36 Cheng et al., 2017 J Physiol Arthur J. Cheng 34
Bottomline: CWI for Recovery? Arthur J. Cheng 20/02/2018 35