ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE

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This active test table informs the user of Biochemistry tests available in house. s referred to other sites are recorded in the Referred Table. Issue date: 4 TH April 2016 Contact Phone Number ext.1345/2522 Page 1! of 11!

Alanine Aminotransferase (ALT) Albumin Alkaline Phosphatase (ALP) Amylase Aspartate Aminotransferase (AST) 2-3 hrs 45 mins Adult Female : <33U/L Adult Male : < 41 U/L Neonates: <45 U/L Non pregnant/male: 35-50 g/l Pregnant: 23-42 g/l Neonate: 32-48 g/l Adult Female : 35-105 U/L Adult Male: 45-130 U/L Pregnant: 20-230 U/L Neonate: <10 days: 45-245 U/L < 4 weeks 45-315 U/L < 1Y 60-550 U/L < 9Y 50-450 U/L <15Y 50-350 U/L Adult: 28-100 U/L Neonate: <100 U/L Adults female: <32 U/L Adult Male: <45 U/L Neonate: < 4 weeks: < 75U/L < 1Y: <65U/L < 4Y <50 U/L Page 2! of 11!

Bile Acids Bilirubin Direct Bilirubin Total Calcium Serum or Minimum 4hr fast. Please state fasting status on request form 2-3 hrs 45 mins Adult Female 0-10 umo/l <5 µmol/l Adult: <21 µmol/l Neonates: <17µmol/L Adults: 2.15-2.50 mmol/l Neonates: 2.15-2.65 mmol/l Chloride Adult: 98-107 mmol/l Neonates: 98-110 mmol/l Urine : No range quoted Cholesterol Overnight fast Adults: <5.2 mmol/l C-Reactive Protein Creatine Kinase (CK) <10 mg/ L Adult Female: 20-180U/L Adult Male 20-200 Neonate: < 2weeks : <600U/L <1 month: <450 U/L <1yr : <300 U/L Page 3! of 11!

Creatinine Fructosamine Gentamicin GGT Glucose Glucose Tolerance Serum or Sodium Fluoride Collection time must be handwritten on tube and on request form. 2-3 hrs Adult Female: 45-85 µmol/l Adult Male: 60-105 µmol/l Pregnant: 45-80 µmol/l < 7 days: <100 µmol/l < 4 weeks: 10-70 µmol/l < 1yr : 10-70 µmol/l < 4 years: 15-45 µmol/l 205-285 µmol/l Dependant on dose and time of dose. Adult Female : 6-42 U/L Adult Male: 10-71 U/L Neonate: <1 month: 0-150 U/L 1-2 month : 0-114 U/L 2-4 month : 0-81 U/L 4-7 month : 0-34 U/L <12yr : 0-24 U/L Adult fasting: < 5.1 mmol/l Pregnant Fasting: < 5.1 mmol/l 1 Hour: < 10 mmol/l 2 Hour: < 8.5 mmol/l Page 4! of 11!

Breakfast Club request form. Haemoglobin A1c EDTA Ethnicity must be included on request form HDL - Cholesterol Overnight fast 1 week 45mins Fasting: < 5 mmol/l 1 hour: < 7 mmol/l Non Pregnant: 20-42 mmol/mol 1.0-2.2 mmol/l LDL - Cholesterol 2.0-3.36 Lactate Dehydrogenase (LDH) 2-3 hours Adult : 245-480 U/L Neonate: <30 d: 365-1450 U/L 30d-6mth: 310-790 U/L 6mth-1yr : 325-670 U/L >1yr - 2yr: 200-565 U/L >2yr - 16yr : 230-600 U/L Lactate Sodium Fluoride Send to the lab immediately 0.5-2.2 mmol/l Magnesium Phosphate Adult: 0.7-1.1 mmol/l Neonates: 0.6-1.0 mmol/l Adult: 0.8-1.45 mmol/l Neonates: 1.5-2.55 mmol/l Potassium Adult: 3.4-4.5mmol/L Neonates: 3.5-5.5 mmol/l Urine : No range quoted Page 5! of 11!

Sodium Total Protein / Urine the 2-3 hours 45 mins Non pregnant: 136-145 mmol/l Pregnant: 133-143 mmol/l Neonate: 133-144 mmol/l Urine : No range quoted Non pregnant: 65-90 g/l Pregnant: 56-76 g/l Neonate: 60-80 g/l Total Protein Urine (24hr collection) 24 hr Urine collection Adult : < 0.15g/L Creatinine Clearance (24hr collection) 24 hr urine & Hep Non Pregnant: 80-125 ml/min Protein / Creatinine Ratio Spot Urine < 15 mg/mmol Cr Normal to mildly increased 15-50 mg/mmol Cr Moderately Increased > 50 mg/mmol Cr Severely Increased NICE guidelines do not apply to pregnant women. PET women with PCR > 30 mg/mmol Cr should be confirmed with 24 hr urine collection CSF Protein CSF CSF 0.1-0.4 g/l CSF Glucose 2.5-4.0 mmol/l Page 6! of 11!

CSF Lactate Triglycerides Urea Uric Acid CSF in sodium fluoride tube Send to the lab immediately Overnight fast 2-3 hours 45 mins Adult : 1.1-2.4 Neonate : 0-3d : 1.1-6.7 mmol/l 3d-10d : 1.1-4.4 mmol/l 10d - 2yrs: 1.1-2.8 mmol/l >2yrs : 1.1-2.4 mmol/l Adults: <2.26 mmol/l Neonate: < 1.7mmol/L Non-pregnant 2.8-8.1mmol/L Pregnant: 1.0-3.8 mmol/l Neonates: 1.0-6.0 mmol/l Non pregnant :145-345 µmol/l Pregnant: 120-375 µmol/l Male: 200-420 µmol/l Neonates 150-330 µmol/l Page 7! of 11!

Endocrinology Page 8! of 11!

Anti Thyroid-Specific Peroxidase AMH Serum Serum Same day (except at weekends) Run twice weekly Urge nt s N/A N/A 0-34kU/L Only performed on 1. Pregnant patients with TSH>2.0 miu/l 2. Non pregnant patients with TSH > 4.0 miu/l Adult Female: 13.1-53.8 20-29 yrs 6.8-47.8 30-34 yrs 5.5-37.4 35-39 yrs 0.7-21.2 45-44 yrs 0.3-14.7 45-50 yrs Neonate Female: 3.6-24 <3m 2.4-15 <12m 1.5-46 <8Y Neonate Male: 111-347 <15d 599-1544 <6m 279-651 <1Y 367-631 <4Y 316-1118 <6Y 257-974 <9Y Accredit ed CA-125 Serum Run twice weekly 0-3 5 ku/l Ferritin Serum Same day (except at weekends) N/A Female: 13-150 µg/l Male: 30-450 µg/l Page 9! of 11!

Folate weekends) Urge nt s Accredit ed 4.6-18.7 ng/ml Follicle Stimulating Hormone Free Thyroxine (FT4) Human ChoronicGonadotrophin Lutenizing Hormone Oestradiol Progesterone Prolactin Sex Hormone Binding Globulin osterone Serum Same day (except at weekends) 2 hours N/A Thyroid Stimulating Hormone 2 hours See table below Adult: 12-22pmol/L < 1 month: 10-36pmol/L < 1 year: 10-26pmol/L Male and non-pregnant females: < 1.0 U/L Pregnant female: See below See table below Female: 100-500 miu/l Male: 85-325 miu/l Female: 32-128 nmol/ll Male: 18-54 nmol/l Female: 0.2-3.0 nmol/l Male: 9-29 nmol/l Adult: 0.1-4.0 miu/l < 2 days: 5.0-45 miu/l < 11 years: 0.1-5.5 miu/l Vitamin B12 N/A Adult: 141-489 pg/ml Page 10! of 11!

s for Fertility Hormones Analyte Unit Male Follicular Phase Midcycle Luteal phase Post-menopausal LH U/L 1-9 2-13 14-96 1-12 8-60 FSH U/L 1-12 3-12 5-25 1-8 25-135 Oestradiol pmol/l - 45-854 - - <183 Progesterone nmol/l 0.7-4.3 0.6-4.7 2.4-9.4 > 20 0.3-2.5 Average HCG (U/L) and days of cyesis (days post LMP) Royal Berkshire Hospital figures: Day HCG Day HCG Day HCG Day HCG 24 50 25 63 26 80 27 102 28 135 29 180 30 245 31 320 32 420 33 545 34 710 35 945 36 1300 37 1700 38 2300 39 3100 45 4300 41 6200 42 8200 43 11000 44 14500 45 18000 45 23000 47 28000 48 34500 49 45000 50 46000 51 51000 52 56000 53 62000 54 68000 55 74500 56 80000 57 86000 58 91000 59 96000 60 101000 Figures are based on data collected between December 1996 and February 1997 in the Endocrinology Department, RBH, from apparently normal pregnancies. There is considerable individual variation, but around 50% of normal pregnancies give HCG values within 3 days of the average day, and around 80% within 1 week of the average day. After 60 days of cyesis, HCG values plateau and being to decline. HCG results cannot therefore be used to predict dates for pregnancies of duration likely to be more than 60 days. Page 11! of 11!