Evaluation of Assail for the Control of Early Season Cotton Aphids in Upland Cotton COOPERATIVE RESEARCH PROJECT 2001

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of Early Season Cotton Aphids in Upland Cotton COOPERATIVE RESEARCH PROJECT 2001 Donald J. Reid, Agronomist Texas A & M University-Commerce James S. Swart, Entomologist Texas Agricultural Extension Service BACKGROUND: The cotton aphid (Aphis gossypii) is considered a secondary pest of cotton in the Northern Texas Blacklands. Normally, the aphid populations are suppressed by beneficial insects, which include ladybeetles and lacewings. In this region, an aphid problem is most often associated with an in-season bollworm/budworm or boll weevil control program. Aphid populations can explode when a non-selective insecticide is used to control one of these primary pests. The TAMU economic threshold for aphids is 50 or more per leaf. High populations that are not controlled will cause a reduction in both boll size and lint yield. OBJECTIVE: The purpose of this experiment was to study the efficacy of Assail for the control of cotton aphid (Aphis gossypii) in upland cotton. This material was compared at various rates to the control standard of uradan. Control ratings were made at 3, 7, and 11 days after treatment (DAT). MATERIALS AND METHODS: A block of cotton (variety: DP 422BG/RR)) was planted on the TAMU-Commerce research farm near airlie on June 12 for this experiment. Starting at the 5-leaf stage, the field was sprayed weekly with a rotation of pyrethroid insecticides at the full-labeled rate to suppress beneficials and create an aphid infestation. On July 26, cotton aphid populations had reached levels in excess of 50 aphids per leaf, and the following insecticide treatments were applied the following day:

Treatment Rate A. I. /Acre Untreated check - Assail 70WP.038 Assail 70WP.05 Assail 70WP.075 Assail 70WP.10 uradan 4.25 At the time of application, the cotton was approximately 12 inches tall and at the first 1/3 grown square stage of development. Each plot consisted of one 38 row, 20 feet long. An untreated row was left between treatments to minimize the border effect. The experimental design was a randomized complete block with six replications. Environmental conditions at the time of application were as follows: temperature 90 ; wind calm; relative humidity - 70%. The applications were made using a three-nozzle boom (19 inch nozzle spacing) with the center nozzle held directly over the row. All insecticides were applied at 20 height through Tee Jet 8002VS flat-fan nozzles in 14.5 gallons of water per acre at 24 psi. A baseline count was made on the uppermost fully expanded leaf of twenty plants in all of the untreated check plots to determine the aphid population at the first rating date. ollowing this estimate, all plots were rated for percent control by a consensus of two people on a scale of 1 100, with 1 representing no control, and 100 designating complete control. Aphid populations were then calculated by subtracting percent control from 100, and multiplying the remainder by the baseline aphid number. Ratings were made at 3, 7, and 11 DAT. The baseline aphid population was used as a basis for all three rating dates. RESULTS AND DISCUSSION: At 3 DAT, all rates of Assail, and the uradan treatment were providing excellent control, and everything was significantly better than the untreated check. There was no rate response with Assail -all rates were performing equally (Summary tables 1 and 2). At 7 DAT, all of the insecticide treatments had improved, and were providing superb control. There were no aphids observed in plots with the three highest rates of Assail, and only a few with the lowest rate.

The rating scheduled for 14 DAT was moved up to11 DAT when it was noted that the aphid population was threatened by an invasion of ladybird beetles. At that time, all of the Assail treatments were providing 100 percent control. CONCLUSIONS: uradan has been the standard of excellence for cotton aphid control in this region. However, since uradan does not have a ederal registration and has only been available under a series of Section 18 s, its availability to the growers is not assured. Assail is the best aphid material we have ever seen. When it receives a federal label, it will provide more dependable aphid control for growers at much lower mammalian toxicities.

Table 1: Number of Cotton Aphids on Uppermost ully Expanded Leaf 3 DAT Treatments (Lb. a. i. /A) 1 2 3 4 5 6 Mean Assail 70 WP @.10 6 6 6 6 6 6 6.0a Assail 70 WP @.075 6 6 6 6 13 6 7.2a uradan 4 @.25 13 6 13 6 6 13 9.5a Assail 70 WP @.038 6 19 19 6 6 6 10.3a Assail 70 WP @.05 13 13 13 6 13 6 10.7a Untreated Check 113 119 113 113 113 119 115.0b (D) Between Subject 82.22 5 Within Subject 56934.67 30 Repeated actor 56563.89 5 11312.78 762.77** <.001 Error 370.78 25 14.83 TOTAL 57016.89 35

Table 2: Percent Aphid Control 3 DAT Treatments (Lb. a. i./a) 1 2 3 4 5 6 Mean Assail 70 WP @.10 95 95 95 95 95 95 95.0a Assail 70 WP @.075 95 95 95 95 90 95 94.2a uradan 4 @.25 90 95 90 95 95 90 92.5a Assail 70 WP @.038 95 85 85 95 95 95 91.7a Assail 70 WP @.05 90 90 90 95 90 95 91.7a Untreated Check 10 5 10 10 10 5 8.3b (D) Between Subject 47.22 5 Within Subject 36108.33 30 Repeated actor 3587.22 5 7179.44 850.20** <.001 Error 211.11 25 8.44 TOTAL 36155.55 35

Table 3: Number of Cotton Aphids on Uppermost ully Expanded Leaf 7 DAT Treatments (Lb. a. i. /A) 1 2 3 4 5 6 Mean Assail 70 WP @.05 0 0 0 0 0 0 0.0a Assail 70 WP @.075 0 0 0 0 0 0 0.0a Assail 70 WP@.10 0 0 0 0 0 0 0.0a Assail 70 WP @.038 0 6 0 0 0 0 1.0a uradan 4L @.25 6 0 6 0 0 0 2.0a Untreated Check 100 81 94 100 63 106 90.7b (D) Between Subject 228.22 5 Within Subject 41708.33 30 Repeated actor 40579.22 5 8115.84 179.70** <.001 Error 1129.11 25 45.16 TOTAL 41936.55 35

Table 4: Percent Aphid Control 7 DAT Treatments (Lb. a. i./a) 1 2 3 4 5 6 Mean Assail 70 WP @.05 100 100 100 100 100 100 100.0a Assail 70 WP @.075. 100 100 100 100 100 100 100.0a Assail 70 WP @.10 100 100 100 100 100 100 100.0a Assail 70 WP @.038 100 95 100 100 100 100 99.2a uradan 4L @.25 95 100 95 100 100 100 98.3a Untreated Check 20 35 25 20 50 15 27.5b (D) Between Subject 150.00 5 Within Subject 26675.00 30 Repeated actor 25933.33 5 5186.67 174.83** <.001 Error 741.67 25 29.67 TOTAL 26825.00 35

Table 5: Number of Cotton Aphids on Uppermost ully Expanded Leaf 11 DAT Treatments (Lb. a. i. /A) 1 2 3 4 5 6 Mean Assail 70 WP @.038 0 0 0 0 0 0 0.0a Assail 70 WP @.05 0 0 0 0 0 0 0.0a Assail 70 WP@.075 0 0 0 0 0 0 0.0a Assail 70 WP @.10 0 0 0 0 0 0 0.0a uradan 4L @.25 6 0 6 0 0 0 2.0a Untreated Check 63 50 44 38 25 50 45.0b (D) Between Subject 179.33 5 Within Subject 10657.67 30 Repeated actor 9965.00 5 1993.00 71.93** <.001 Error 692.67 25 27.71 TOTAL 10837.00 35

Table 6: Percent Aphid Control 11 DAT Treatments (Lb. a. i./a) 1 2 3 4 5 6 Mean Assail 70 WP @.05 100 100 100 100 100 100 100.0a Assail 70 WP @.075. 100 100 100 100 100 100 100.0a Assail 70 WP @.10 100 100 100 100 100 100 100.0a Assail 70 WP @.038 100 100 100 100 100 100 100.0a uradan 4L @.25 95 100 95 100 100 100 98.3a Untreated Check 50 60 65 70 80 60 64.2b (D) Between Subject 150.00 5 Within Subject 26675.00 30 Repeated actor 25933.33 5 5186.67 174.83** <.001 Error 741.67 25 29.67 TOTAL 26825.00 35

01-08s. Evaluation of Assail for the Control of Early Season Cotton Aphids in Upland Cotton COOPERATIVE RESEARCH PROJECT 2001 Donald J. Reid, Agronomist Texas A & M University-Commerce James S. Swart, Entomologist Texas Agricultural Extension Service Summary Table 1: Number of cotton aphids on uppermost fully expanded leaf Treatment Rate a. i. /A 3 DAT 7 DAT 11 DAT Assail 70WP.10 lb. 6.0a 0.0a 0.0a Assail 70WP.075 lb. 7.2a 0.0a 0.0a uradan 4.25 lb. 9.5a 2.0a 2.0a Assail 70WP.05 lb. 10.7a 0.0a 0.0a Assail 70WP.038 lb. 10.3a 1.0a 0.0a Untreated check - 115.0b 90.7b 45.0b P value <.001 <.001 <.001 Summary Table 2: Percent Aphid Control Treatment Rate a. i. /A 3 DAT 7 DAT 11 DAT Assail 70WP.10 lb. 95.0a 100.0a 100.0a Assail 70WP.075 lb. 94.2a 100.0a 100.0a uradan 4.25 lb. 92.5a 98.3a 98.3a Assail 70WP.05 lb. 91.7a 100.0a 100.0a Assail 70WP.038 lb. 91.7a 99.2a 100.0a Untreated check - 8.3b 27.5b 64.2b P value <.001 <.001 <.001