Primary Mouse Cerebral Cortex Neurons V: 80% TE: 70%

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Primary Mouse Cerebral Cortex Neurons V: 80% TE: 70% Pictures: 9 days after electroporation Red: MAP2 Blue: GFAP Green: GFP The cells were from Embryonic Day 14 Mouse Cerebral Cortex

Primary Mouse Hippocampal Neurons Pictures: 3 days after electroporation Red: MAP2 Green: GFP

Primary Mouse Neural Progenitor Cells V: 80% TE: 60% Pictures: 7 days after electroporation Red: Phalloidin Blue: DAPI Green: GFP

Primary Mouse Cerebellar Granule Neurons Nucleofector (Lonza amaxa) result: Transfection Efficiency 24% TE: 65%

Primary Rat Cerebral Cortex Neurons V: 70% TE: 75% The cells were from Embryonic Day 16 Rat Cerebral Cortex. Pictures: 5 days after electroporation

Primary Rat Bulbar Neurons V: 80% TE: 75% 7 days after electroporation / The cells were from Embryonic Day 15 Rat Bulbar Neurons

Primary Rat Schwann cells V: 90% TE: 80%

Primary Rat Schwann cells V: 90% TE: 60% Pictures: 48 hours after electroporation

Primary Chick Dorsal Root Ganglia V: 90% TE: 40% The cells were from day 7-9 chick embryos

Primary Chick Dorsal Root Ganglia V: 90% TE: 40% The cells were from day 7-9 chick embryos

Mouse Neurospheres V: 90% TE: 75% The cells were from E13.5 mouse brain: ganglionic eminence

Mouse Neurospheres Neural stem cells derived from mouse SVZ 24 hours after electroporation 48 hours after electroporation 72 hours after electroporation

Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation Many robust EGFP signals suggest high transfection efficiency.

Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation *High magnification Neurites are shown clearly. Department of Neurochemistry, National Institute of Neuroscience, Japan

Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation *High magnification Neurites are shown clearly. Department of Neurochemistry, National Institute of Neuroscience, Japan

Primary Mouse Cerebral Cortex Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates

Primary Mouse Cerebral Cortex Neurons in Adherence V: 60% TE: 65% The neurons were prepared from E16 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates

Primary Mouse Cerebral Cortex Neurons in Adherence V: 95% TE: 75% The neurons were prepared from E14 mouse cerebral cortex. Electroporation: After 5 days in vitro (DIV) on 24-well plates

Primary Mouse Hippocampal Neurons in Adherence V: 85% TE: 54% The neurons were prepared from E18 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates

Primary Mouse Microglial cells in Adherence V: 80% TE: 73% Electroporation: After 1 days in vitro (DIV) on 24-well plates, post 1-week co-culturing astrocyte and microglial cells

Primary Mouse Glial cells in Adherence Electroporation: After 14 days in vitro (DIV) V: 80% TE: 50%

Primary Rat Cerebral Cortex Neurons in Adherence V: 70% TE: 60% The neurons were prepared from E17 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 24 hours after electroporation

Primary Rat Hippocampal Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation

Primary Rat Hippocampal Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 4 days after electroporation

Primary Rat Hippocampal Neurons in Adherence V: 100% TE: 50% 48 hours after electroporation The neurons were prepared from P7 rat hippocampus. Electroporation: After 11 DIV

Primary Rat Hippocampal Neurons in Adherence V: 100% TE: 50% 48 hours after electroporation The neurons were prepared from P7 rat hippocampus. Electroporation: After 11 DIV