SRI NATHELLA SAMPATHU CHETTY CLINICAL LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) Test Master List

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S. No Lab/ Ref/ code Name of the Test CLINICAL BIOCHEMISTRY TESTS MASTER LIST Storage of Specimen Anti Reportable examined Required Coagulants interval specimen / (vacutainer) (vacutainer) (TAT) Temp. Information to the patients 1. 102 Plasma Glucose F/R/PP 2 ml Fluoride Same day 24 hours 2. 103 Glucose Tolerance test (GTT) 2 ml Fluoride Same day 24 hours 3. 105 Plasma 4 ml Heparin Same day 24 hours Urea/Creatinine 4. 106 Serum Cholesterol 4 ml Plain/heparin Same day 24 hours 5. 4 ml plain Same day 24 hours 107 Serum Uric acid 6. Plasma Sodium and 4 ml Heparin Same day 24 hours 108 Potassium F- Fasting- without food. R- Random-(any time) PP- Postprandial blood should be collected after lunch. Should not exceed 2 hours time. First blood should be collected in. Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 1 of 7

7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 109 110 111 112 Plasma Sodium, Potasium, Chloride and Bicarbonate Serum Calcium and Phosphorus Serum Iron and Iron Binding Capacity Serum Ceruloplasmin 113 Serum LFT 114 115 116 117 118 Serum Bilirubin (Total/Direct) Serum Total Protein and albumin Serum Alkaline Phosphatase Alanine amino transferase Aspartate amino transferase 4 ml Heparin Same day 24 hours Should not take iron medication for 3 days. 4 ml Plain 3 days 3 days / 4 C 4 ml plain Same day 24 hours 4 ml plain Same day 24 hours Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 2 of 7

17. Lipid profile Total Cholesterol 123 HDL cholesterol Triacylglycerol 18. 124 Plasma Triacyl Glycerol (TGL) 19. 126 Angiotensin converting enzyme (ACE) 20. 127 Urine Inborn errors of metabolism 21. 128 CSF sugar, protein and chloride 22. 135 Amino acid profile plasma 23. 136 Amino acid profile- Urine /- 20 C - - Same day Discarded on the same day CSF-1.0ml Same day 24 hours/ ACD 300 µl 7 days 1 week// -20 C 7 days 1 week/ -20 C Blood should be collected in Blood should be collected in Provide 3 urine containers with pinch of sodium azide. 1 for fasting and 2 for Random Should be received on the same day. Blood should be collected in Urine containers should be provided with pinch of sodium azide. sample should be collected in Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 3 of 7

24 122 Urine- Homocystinuria screening Same day 1 week/ 4 C Urine containers should be provided with pinch of sodium azide. Sample should be collected in. Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 4 of 7

SPECIAL BIOCHEMISTRY INVESTIGATIONS MASTER LIST Specimen Specimen Code Required Name of the Test Required No (vacuutaine (manual) r) S.No Anticoagulan t Reportable intervals Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 5 of 7 Temp./Storage of examined specimen Information to the patients 1 131 Serum Vitamin A 4 ml plain 4 ml plain - 3 days -20 o C/3 days Should not eat vitamin tablet, green leaf & carrot. 2 138 Plasma Homocysteine plasma (ELISA) 3 140 Serum Thyroid profile: T3, T4, TSH 4 121 Electrophoresis for protein serum/ urine/csf 5 122 Electrophoresis for serum/ Lipoprotein 2 ml (fasting) 2 ml (fasting) EDTA 10 days -20 o C/ 3 days Blood should be condition 4 ml plain 4 ml plain - Before 12pmsame day After 12pmnext day. 4 ml blood (plain) / 5 ml urine sample/1ml CSF 4 ml blood (plain) -20 o C/3 days plain - 2 days -20 o C/ 3 days 4 ml plain - 2 days -20 o C/ 3 days Fasting condition

6 125 Blood Pyruvate and Lactate 7 129 Blood Glucose 6 phosphate Dehydragenase 8 130 Serum Lactate dehydragenase 9 133 Paper chromatography for Galactose/any sugars 10 134 Paper chromatography for Ornithine / amino acids 2 ml whole blood - 4ml of 0.6M per chloric acid. 3 ml 4 ml Heparin/ EDTA 2 days -20 o C/ 3 days 2 days 24hours 3 ml plain 4 ml Plain 2 days 24hours 4ml blood / 5 ml urine (fasting followed by pp) 2ml blood/ 5 ml urine (fasting) 4ml blood Heparin 3 days -20 o C/ 3 days Fasting followed by postprandial after baby feed with milk Post prandial blood to be collected for galactose 4 ml blood ACD 300 µl 3 days -20 o C/ 3 days Blood should be condition 11 137 Serum amylase 4 ml (plain) 4 ml (plain) - 3 days -20 o C/3 days - 12 141 Serum Vitamin E 4 ml (plain) 4 ml (plain) - 3 days -20 o C/3 days - Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 6 of 7

13 142 Plasma vitamin C 4 ml 4 ml heparin 2 days 24hours - 14 143 Blood Glutathione 2 ml 2 ml EDTA 3 days -20 o C/3 days - 15 144 Blood Glutathione 2 ml 2 ml EDTA 3 days -20 o C/3 days - Peroxidase 16 145 Blood Superoxide 4 ml 4 ml heparin 3 days -20 o C/3 days - dismutase 17 146 Blood Thio 2 ml 2 ml EDTA 3 days -20 o C/ 3 days - Barbituric Acid Reactive Substances 18 151 HbA1c 2ml 2ml EDTA 2 days 24hours Blood should be /Random condition 19 152 Micro albumin 25ml urine (Sterile urine container) 2days 24hours Urine should be condition 20 153 Minerals 4ml plain 4ml plain Plain 2-20days 24 hours Should call biochemistry department before the collection. Issue No : 7 Issue Date : 5.12.12 Amend No: 6 Page 7 of 7