21 8 1 2 1 1 1 1 1 1* 1. 100029 2. 100700 SHR 14 ~ 18 14 SHR 55 SHR WKY ELISA 4 6 5-5-HT Ⅱ ANGⅡ NE 14 ~ 20 P < 0. 05 5-HT ANGⅡ NE 4 6 P < 0. 05 14 ~ 18 5-HT ANGⅡ NE 14 ~ 18 SHR 5-HT ANGⅡ NE R285. 5 doi 10. 13422 /j. cnki. syfjx. 2015080097 A 1005-9903 2015 08-0097-05 Research of Relative indexes of Animal Models of Hypertension with Liver Fire Hyperactivity Syndrome XUE Xiao-xing 1 LI Yu-bo 2 LIAN Hong-jian 1 LI Jun-ling 1 YAN Jing-lu 1 MA Xue-ling 1 WANG Wei-ming 1 WANG Wei 1* 1. School of Preclinical Medicine of Beijing University of Chinese Medicine Beijing 100029 China 2. Institute of Basic Theory of Traditional Chinese Medicine of Chinese Academy of Chinese Medical Sciences Beijing 100700 China Abstract Objective To investigate the biological basis of hypertension with hyperactivity of liver fire of macro characterization and behavioral tests micro physicochemical indexes disproof prescriptions. Method Fifty-five SHR rats with hyperactivity of liver fire were divided into model group Sancao Jiangya decoction SCD group Sini San decoction SND group and nifedipine group according to Latin square method Wistar-Kyoto WKY rats as control group. The macro characterization behavioral indexes and the systolic blood pressure SBP were collected. Enzyme-linked immunosorbent assay ELISA was used to detect the serum concentration of 5-hydroxytryptamine 5-HT angiotensin Ⅱ ANG Ⅱ norepinephrine NE after the rats were administrated for 4 and 6 weeks. Result Compared with control group the SBP and irritable degree increased revolving tolerance time decreased and serum 5-HT ANGⅡ NE level was higher in model group. Compared with model the SBP in SCD group and nifedipine group reduced after treated for 4 6 weeks 18 20 weeks of age the SBP in SND group was stable and increased in the 16 weeks and then decreased. The symptoms of liver fire hyperactivity improved and it was not obvious in SND group and serum 5-HT ANG Ⅱ NE level was lower in SCD group compared with model group. Conclusion Fourteen-eighteen weeks is the stable time window of hyperactivity of liver fire. SHR 5-HT ANGⅡ NE may be used as the objective index of hypertension of hyperactivity of liver fire syndrome. 20140926 010 973 2011CB505106 2014-JYBZZ-XS-026 Tel 64286508 E-mail xuexiaoxing2012@ 126. com * Tel 010-64286508 E-mail wangwei@ bucm. edu. cn 97
21 8 Key words spontaneously hypertensive rats hyperactivity of liver fire biological mechanism 6. 25 60 kg ig 10 ml kg - 1 9 SHR 14 ~ 18 g kg - 1 2. 5 g kg - 1 0. 002 g kg - 1 1 ig 2. 3 SBP 3 2-4 1 7 d 5-5-HT Ⅱ ANGⅡ 2. 4 NE 5 5-5-HT Ⅱ ANGⅡ NE 1 1. 1 13 SHR 55 WKY 12 SPF 60 r min - 1 SCXK 2012-0001 2 min 2. 4. 1 2. 4. 2 1 7 1. 2 SoftronBP-98A Ⅲ JD-01GSZ 3 Ⅱ 2 Ⅰ 1 5-HT ANGⅡ NE ELISA 0 0 RD EIA-3325 2. 4. 3 1 7 3667 EIA-3586 1. 3 20 g 20 g 20 g 20 g 6 g 6 g 6 g 6 g 6 g 2. 5 5-HT ANGⅡ NE 4 6 10% 4 ml kg - 1 5 ml H11022296 1 ~ 2 h 3 000 r min - 1 4 15 min ELISA 2 5-HT ANGⅡ NE 2. 1 SHR WKY 1 2. 6 SPSS 20. 0 x 珋 ± s 1 6 ANOVA t P < 0. 05 SHR 3 3. 1 WKY 4 6 2. 2 14 ig 1 /d 6 P < 0. 05 98
21 8 4 6 P < 0. 05 1 1 Table 1 x 珋 ± s Comparison of SBP in rats of all groups in different administration time 珋 x ± s n = 12 /mmhg 2 n = 12 4 n = 12 6 n = 6-169. 4 ± 13. 6 188. 1 ± 6. 5 2 202. 2 ± 6. 8 2 205. 0 ± 6. 9 2 9 169. 4 ± 13. 7 184. 4 ± 9. 6 2 178. 6 ± 6. 9 1 176. 8 ± 1. 17 1 2. 5 169. 6 ± 13. 9 200. 3 ± 10. 8 1 2 190. 1 ± 5. 3 2 195. 3 ± 5. 2 2 0. 002 168. 9 ± 13. 6 182. 7 ± 13. 9 2 188. 9 ± 12. 1 1 2 170. 8 ± 12. 2 1-111. 2 ± 7. 7 1 110. 0 ± 10. 3 1 113. 6 ± 7. 4 1 116. 7 ± 6. 0 1 1 P < 0. 05 2 P < 0. 05 2 1 mmhg = 0. 133 kpa 3. 2 14 55 SHR 48 87. 2% 33% 2 16 4 18 16 18 16 18 12 7 58. 3% 20 6 3. 3 3 50% 2 P < 0. 05 16 12 8 77% 4 6 4 18 12 6 50% 6 20 6 2 6 20 3 50% P < 0. 05 2 2 Table 2 x 珋 ± s Comparison of irritable degree in rats of all groups in different administration time 珋 x ± s n = 12 / 2 n = 12 4 n = 12 6 n = 6-1. 60 ± 0. 52 1. 90 ± 0. 74 2. 50 ± 0. 53 2 2. 40 ± 0. 70 2 9 1. 67 ± 0. 71 2. 11 ± 0. 87 2 2. 00 ± 0. 50 1 2 1. 89 ± 0. 78 1 2 2. 5 1. 56 ± 0. 53 2. 11 ± 0. 60 2 2. 22 ± 0. 67 2 2. 11 ± 0. 78 2 0. 002 1. 50 ± 0. 53 2. 00 ± 0. 67 2 2. 20 ± 0. 63 2 2. 20 ± 0. 79 2-0. 25 ± 0. 46 1 0. 38 ± 0. 52 1 0. 25 ± 0. 46 1 0. 25 ± 0. 46 1 3. 4 20 4 18 P < 0. 05 P < 0. P < 0. 05 4 2 3. 5. 3 NE 6 20 P < 0. 05 6 4 4 18 P < 0. 05 3 3. 5 3. 5. 1 5-HT 18 6 20 6 20 4 P < 0. 05 3. 5. 2 ANGⅡ18 ANGⅡ P < 0. 05 6 P < 0. 05 NE 5-HT P < 0. 05 5-HT 4 8 9 99
21 8 3 Table 3 x 珋 ± s Comparison of revolving tolerance time in rats of all groups in different administration time 珋 x ± s n = 12 /s 2 n = 12 4 n = 12 6 n = 6-25 ± 6 28 ± 7 22 ± 5 24 ± 5 9 20 ± 7 25 ± 4 34 ± 4 1 2 37 ± 9 1 2 2. 5 23 ± 11 24 ± 8 25 ± 2 30 ± 4 0. 002 24 ± 10 27 ± 13 22 ± 6 29 ± 14-88 ± 31 2 91 ± 33 2 90 ± 23 2 109 ± 17 2 4 Table 4 14 1 P < 0. 05 2 P < 0. 60 r min - 1 2 min 120 s 5-HT ANGⅡ NE x 珋 ± s n = 6 Comparison of concentration of serum 5-HT ANGⅡ NE in rats of all groups in different administration time x 珋 ± s n = 6-1 5-HT /μg L ANGⅡ /ng L - 1 NE /μg L - 1 4 6 4 6 4 6-21. 3 ± 3. 4 14. 3 ± 2. 2 1 147. 1 ± 17. 4 89. 9 ± 37. 3 1 22. 7 ± 13. 1 43. 9 ± 5. 0 1 9 17. 4 ± 5. 5 14. 8 ± 2. 4 1 69. 6 ± 29. 8 2 72. 6 ± 22. 6 15. 7 ± 4. 8 2 28. 9 ± 9. 0 2 2. 5 18. 9 ± 2. 2 16. 3 ± 3. 9 1 75. 3 ± 0. 6 2 120. 4 ± 14. 7 1 17. 9 ± 8. 8 2 50. 6 ± 15. 1 1 0. 002 18. 6 ± 2. 8 14. 9 ± 2. 5 1 116. 3 ± 19. 8 2 87. 4 ± 25. 1 1 41. 5 ± 10. 0 2 64. 2 ± 11. 4 1 2-19. 8 ± 3. 9 15. 3 ± 3. 4 1 65. 6 ± 40. 4 2 74. 0 ± 28. 7 17. 2 ± 6. 3 2 23. 3 ± 9. 6 2 4 18 1 P < 0. 05 2 P < 0. 3 4 5-HT ANGⅡ NE 5-HT ANGⅡ NE SHR 14 ~ 18 SHR 14 ~ 18 SHR14 ~ 18 1. 5-HT 2013 8 2 134-137. 5-HT2A 10 K + 2. J. 11 ANG Ⅱ NE 12-13 2007 4 20 129-130. 3. 5-HT 5-HT J. 2006 27 5 63-65. 5-HT 14-15 4. J. 2013 RAS ANG Ⅱ 7 7 121-124. RAS ANG Ⅱ 5. ANGⅡ H 2 O 2 J. 2013 28 NE 16 10 2897-2900. ANGⅡ NE 6. 17 100 J. J. 2011 27
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