Sensitive, Accurate and Precise MS Solutions Through SGE Products Diamond MS Syringes evol XR MEPS SilTite FingerTite GC Capillary Columns AUSTRALIA & PACIFIC REGION EUROPE JAPAN USA
Introduction Bringing you a range of products to suit your MS analyses, SGE provides the highest quality syringes, ferrules and columns. Diamond MS Syringes The Choice for Sensitivity Improve the sensitivity of your MS analysis: Lowest syringe carryover Reduced non specific sample interaction and increased analyte recovery Ultra smooth removable needles with choice of surface treatment Direct MS Infusion Rapid and Sensitive Sample Preparation, Direct Infusion into ESI MS Coupling MEPS technology with a digitally controlled analytical syringe, evol MEPS semi-automates sample preparation from extraction through to analysis. SilTite FingerTite The Smart Alternative The next generation ferrule system for gas chromatography systems delivering an easy, leak free installation for capillary columns without the use of any tools. GC Capillary Column Range The High Five The complete polysilphenylene and polycarborane GC column range New BP5MS optimized silphenylene column Five selectivities to suit your analyses
Diamond MS Syringes The Choice for Sensitivity Improve the sensitivity of your MS analysis: Lowest syringe carryover Reduced non specific sample interaction and increased analyte recovery Ultra smooth removable needles with choice of surface treatment We understand how important your MS results are, and how precious your sample is, so we engineered new syringes and needles to bring you the Diamond MS Syringe range. Diamond MS syringes and needles are MS ready and designed to improve the sensitivity of your MS results. Lowest Syringe Carryover The unique design of Diamond MS syringes and removable needles ensures carryover is reduced to levels lower even than the best fixed needle syringes available. In the event of needle damage, Diamond MS needles provide a cost effective alternative to purchasing an entire fixed needle syringe. Diamond MS syringes have unbeatable cleanability thereby further reducing carryover. Diamond MS Syringe Design SilTite metal ferrule for zero adhesive and zero interaction Specialized seal eliminates dead volume Clear visibility of plunger at zero position Color coded needle design Precise needle alignment for 0% sample transfer Reduce Non-Specific Interaction and Increase Sample Recovery Anything in the sample flowpath can interact with your sample and impact your results, so SGE has developed Ultra Smooth needles to reduce non specific interaction between your sample and the needle. 3 Ultra Smooth needles available for you to choose based on your sample properties: Ultra Smooth Ultra Smooth with Hydrophilic Treamtment Ultra Smooth with Hydrophobic Treatment To reduce non specific sample interaction even further, there are two needle treatment options available to avoid sample interactions due to hydrophilicity and hydrophobicity.
Reduce Non-Specific Interaction and Increase Sample Recovery Number of peaks above,000 counts ( LOG scale ) 000 00 0 1 Conventional Syringe Reduced Non -Specific Interaction / Carryover Diamond MS syringe with Ultra Smooth Hydrophilic Coated Needle Diamond MS syringe with Ultra Smooth Needle Diamond MS syringe with Ultra Smooth Hydrophobic Coated Needle Clean syringes were washed 6 times with neat methanol, each wash was collected. Samples were then placed in an Eksigent Tempo LC system and the loading pump (50% methanol/0.1% Formic acid/water) used to infuse (initial flow of 2 µl/min then reduced to 0.5 µl/min for analysis) via a nanospray III ion source into an AB Sciex QTRAP 5500. Spectra were collected for a positive EMS scan of 0-00 amu. Diamond MS syringes are 0 times more sensitive than a conventional syringe. The Choice for Sensitivity How to choose your MS ready syringe and needle for improved sensitivity: 1. Choose a manual syringe or one specifically designed for your instrument 2. Select the syringe capacity 3. Choose the needle geometry LC or GC injections, tip design and needle dimensions 4. Choose which 'Ultra Smooth' needle best suits your application SYRINGES All syringes have a PTFE Plunger Tip Part Number Volume (ul) Manual Syringes 002750 003750 25 004750 50 005750 0 006750 250 007750 500 Agilent Autosampler Syringes 002760 003760 25 Shimadzu Autosampler Syringe 002780 CTC PAL Autosampler Syringes 002770 003770 25 004770 50 005770 0 Thermo RSH Autosampler Syringes 002790 003790 25 004790 50 005790 0 For more information on this product visit www.sge.com/products/syringes/diamond-ms-syringes
Direct MS Infusion Rapid and Sensitive Source Analyzer Multiplier MS Infusion evol and µspe Use evol where ever you would use a syringe or syringe pump - preparation of standards, precise dispensing, dilution, injection, titration, sample preparation, MEPS (micro SPE) AND... direct MS infusion. Comparative results and additional benefits: Significant cost savings vs infusion pump Convenient sample and calibrant introduction by direct infusion Very low and controlled flow rate Syringe Pump and evol Flow Profiles - 500 µl Syringe 5 µl/min Portable does not require constant connection to a power source Program and save methods Compact Arbitrary Intensity Arbitrary Intensity Syringe Pump evol Conditions: 226.1915 m/z Na (NaCOOH) 3, n = 3 replicates of each run. Positive ion mode, Mass range 0-700 m/z. End plate voltage -450 V, Capillary voltage -5000 V, Nebuliser 16 psi, Dry gas 5.0 ml/min, Dry temperature 180 C, External calibration sodium formate. SPE is well known as a preparative technique for sample analysis using GC-MS or LC-MS. Opiate detection in Urine evol MEPS provides fast, easy sample preparation and the digital syringe format allows convenient sample introduction by direct infusion into the ESI-MS. Below is the analysis of opiate metabolites extracted from Urine using reversed-phase MEPS and infused directly into the ESI-MS source. MS Conditions Positive ion mode Mass range 0-550 m/z. End plate voltage -500 V Capillary voltage - 4500 V Nebuliser 25 psi Dry gas 4.0 ml/min Dry temperature 180 C External calibration sodium formate 476.1917 ± 0.005 462.1557 ± 0.005 300.1600 ± 0.005 286.1427 ± 0.005 272.1285 ± 0.005 Masses Codeine-6-Glucuronide 476.1915 m/z Morphine-6-Glucuronide 462.1759 m/z Morphine-3-Glucuronide 462.1759 m/z Codeine 300.1594 m/z Morphine 286.1437 m/z Norcodeine 286.1437 m/z Normorphine 272.1286 m/z Figure 2. Extracted Ion Chromatogram of Dilute Urine Processed Using MEPS and Infused Directly into the ESI Source.
SilTite FingerTite The Smart Alternative This next generation GC ferrule has been designed to be installed only with the force of your fingers (no tools required) delivering an easy, leak and maintenance free seal. Why is it a next generation connection? 0 % Graphite Graphite Vespel SilTite FingerTIte Mechanical Strength Soft Hard Hard Temperature Limit 450 C 350 C No practical limit Grip strength on column Soft Moderate Strong Doesn t need retightening after installation x Ideal for GC-MS applications (minimal porosity) x No tools required for installation and removal x x Permanent positioning of ferrule x x / No deterioration x x Cannot break the capillary column with over-tightening x x No column creep x x Chemically inert x x Who benefits from this product? GC-MS users SilTite FingerTite has been developed with the unique, leak and air free properties of SilTite ferrules, resulting in superior air tightness to reduce background noise in sensitive MS applications so you can use them with confidence. % Response of Base Fragment 69 m/z Graphite Vespel ferrule 0 20 0 Relative Abundances: 28/69 = 9.08 Nitrogen % 32/69 = 2.56 Oxygen % Air 15 20 25 30 35 40 45 50 55 60 65 70 75 m/z Figure 1. MS trace using a Graphite Vespel ferrule after 5 temperature cycles. SilTite FingertTite ferrule % Response of Base Fragment 69 m/z 0 20 0 Relative Abundances: 28/69 = 0.97 Nitrogen % 32/69 = 0.39 Oxygen % No Air 15 20 25 30 35 40 45 50 55 60 65 70 75 m/z Figure 2. MS trace using a SilTite FingertTite ferrule after 5 temperature cycles. (Using an MS, no leaks can be detected, even after 400 temperature cycles between 70 ºC and 400 ºC).
GC Capillary Column Range The High Five The complete polysilphenylene and polycarborane GC column range New BP5MS optimized silphenylene column 5 Selectivities to suit your analyses A Variety of Selectivities to Suit Your Analyses Introducing the new BP5MS GC column completing the SGE High Five range of capillary columns. BP5MS Optimized Silphenylene content for general purpose MS analyses BP5 For general purpose analyses BPX5 Extremely inert, suitable for general purpose analyses, ideal for trace analysis HT5 Unique phase for high temperature applications HT8 The preferred column for polychlorinated biphenyl (PCB) compounds Optimized Silphenylene Content for General Purpose MS Analyses Evaluating Organo Chlorine Pesticides (OCP) on BP5MS. Instrument: TRACE GC/POLARIS-Q Carrier gas: He (1.5 ml/min) Injector: Split/Splitless mode Injector Temp: 275 C Split Mode: Splitless (1 min split valve closed) Split Flow: 30 ml/min Column: 30 m x 0.25 mm x 0.25 µm BP5MS (P/N 0543) Oven Temperature: 60 C (5 min) 8 C/min 300 C ( min) MS Transfer Line Temperature: 300 C MS Type: ITD MS Source Temperature: 225 C MS Acquisition Mode: Segmented Scan 45 450 amu
GC Column Comparison Table Overview Perfect for your 5% MS analysis. Non polar, 5% equivalence. Low bleed, great inertness Minimum Temperature ( C) Maximum Continuous Temperature ( C) Maximum Cycling Temperature ( C) Phase Structure BP5MS BP5 BPX5 HT5 HT8 A general purpose phase with excellent high temperature characteristics. Popular column range used for a wide variety of applications A highly inert phase, suitable for MS use. It has been designed for robustness and is suitable for over 80% of all routine analyses performed by chromatographers Unique high temperature phase suited for simulated distillation and other petroleum applications -40-60 -40-20 330 320 360 380 360 350 340 370 400 370 High temperature and low bleed column for polychlorinated biphenyl (PCB) compounds Separates PCB s on ortho-ring substitution as well as boiling point Industry Suited Trace Analysis General Purpose General Purpose General Purpose Fuels, Environment, Food Environment Optimized for MS analysis Yes - Yes - - Polarity Non-polar Non-polar Non-polar Low polarity Low polarity Solvent Rinsable - - - Yes - Ordering Information For more information on SGE MS specialized products, visit www.sge.com, contact your local SGE ofiice or your local distributor. BR-0000-H SGE Analytical Science Pty Ltd 05/2012 AUSTRALIA & PACIFIC REGION SGE Analytical Science Pty Ltd 7 Argent Place, Ringwood Victoria 3134 Australia Toll Free: 1800 800 167 Tel: +61 (0) 3 9837 4200 Fax: +61 (0) 3 9874 5672 Email: support@sge.com JAPAN SGE Japan Inc RK CUBE 3F 6-85, Ohta-machi Naka-Ku, Yokohama-shi Kanagawa 231-0011 Japan Tel: +81 45 222 2885 Fax: +81 45 222 2887 Email: japan@sge.com EUROPE SGE Europe Ltd European Head Office 1 Potters Lane Kiln Farm Milton Keynes MK11 3LA United Kingdom Toll Free: 00800 2790 8999 Toll Free Fax: 00800 2626 2609 Tel: +44 1908 568 844 Fax: +44 1908 566 790 Tel France: +33 1 69 29 80 90 Fax France: +33 1 69 29 09 25 Tel Germany: +49 (0) 6155 / 60746 0 Fax Germany: +49 (0) 6155 / 60746 50 Email: europe@sge.com UNITED STATES OF AMERICA SGE Incorporated 2007 Kramer Lane Austin Texas 78758 USA Toll Free: (800) 945 6154 Tel: +1 512 837 7190 Fax: +1 512 836 9159 Email: usa@sge.com