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Determination of First Limiting Physical Factors in Corn Silage Hybrids: Modeling Multiple Pools of andfom Mike Van Amburgh, Mike Dineen, Andrew LaPierre, Joe Lawrence, Allison Kerwin and Tom Overton Dept. of Animal Science mev1@cornell.edu 67-592-1212 Overview of Today s Discussion Rumen fill, NDFom and undf Description of corn silage data set Modeling multiple pools in CNCPSv7 based on rumen fill and flux using limits established in previous studies (undf) or provided by Mertens (total NDF) Summarize NDF in CNCPS using lignin*2.4 to estimate undf Traditional single time point: Used 3 h NDFd: 31.3 % of NDF Lignin 2.4 as estimation of indf: 29.3 % of NDF Kd: 2.13 %/h NDF in CNCPS using undf24 3 time point 3h: 31.3 % 12h: 36.5 % 24h: 38.4 % Measured undf 61.6 % of NDF Kd: 5.7 %/h 3 4

NDF residue Corn silage example: NDF 1..8.6 P1+P2+iNDF.4.2 Corn silage example: P1+P2+iNDF NDF residue 1..8.6.4 k d =5%.2 P1 P2 indf P1+P2+iNDF. 5 1 15 2 25 time, hrs. 5 1 15 2 25 time, hrs 5 6 NDF residue Corn silage example: fast pool 1..8.6.4.2. Larger fast pool appears to result in: Faster eating Faster ruminal disappearance not passage Higher intakes More ruminal buoyancy greater chewing and rumination P1 k 1 =11% P1 = 72% NDF 5 1 15 2 25 7 time, hrs NDF residue Corn silage example: slow pool 1..8.6.4.2. P2 Larger Slow and undf pools: More ballast Lower intake Slower eating speed k 2 =2%, P2 = 18.1% NDF 5 1 15 2 25 time, hrs 8

NDF residue 1..8.6.4.2 Corn silage example: indf indf undf k undf =%, undf = 9.9% NDF For comparison 2.4*3% lignin/42% NDF = 17% unavailable NDF Corn Silage Data Set Corn silage test set from 217 648 hybrids Analyzed for chemical composition Evaluated through CNCPS v7 at constant andfom and undf rumen fill allows for evaluation of potential intake effect. 5 1 15 2 25 time, hrs 9 Kerwin, Lawrence, Overton Seed companies that participated in this hybrid evaluation process. Hubner Seed Schlessman Hybrids Growmark FS Dekalb Pioneer Dairyland Seed NK Syngenta Masters Choice Dyna-Gro King Fisher Seedway Augusta Seed Wolf River Valley Seeds Doelber's Channel Dyna-Gro The agronomic conditions are described in the paper 6 5 4 3 2 1 andfom of Corn Silage Hybrids

6 Starch Content of Corn Silage Hybrids 6 5 andfom vs. Starch in Corn Silage Hybrids Starch (% DM) 6 5 Starch (% DM) 5 4 3 2 4 3 2 4 3 2 Starch (% DM) 1 1 1 undfom24 (% DM) 3 25 2 15 1 5 Corn Silage Hybrids Sorted by undfom24 undfom (% DM) 3 25 2 15 1 5 undfom24 of Corn Silage Hybrids sorted by andfom

6 5 4 3 2 1 andfom vs. undfom24 undfom24 (%DM) 6 5 4 3 2 1 undfom24 (% DM) 6 5 4 3 2 1 undfom24 vs. andfom undfom24 (% DM) 6 5 4 3 2 1 undfom24 (% DM) Rumen fill and function What factors play a role in the ability of the rumen to stay full? Internal/Intrinsic factors Pool sizes Digestibility In Digestion rate Passage rate Particle size External factors Feed availability Cow time budget Feed quality palatability, etc What about andfom intake Guidelines provided by Dave Mertens over the last 2 years. How much andfom should a 75 kg cow have in her rumen under steady state conditions? Rule of thumb says ideally she should be consuming 1.25% of BW andfom Miner data ranges from 1.27 to 1.47% If so, then 9.4 kg at steady state? How much should be in her rumen at any one time?

NDF intake as percent BW Week of lactation First Lactation Second + Lactation 2.78.87 4.91 1. 8 1.5 1.17 12 1.12 1.26 16 1.14 1.29 2 1.14 1.3 24 1.13 1.27 28 1.11 1.24 32 1.8 1.19 36 1.4 1.13 4 1.1 1.8 44.97 1.1 Dry cows.92.95 Mertens, 29 Animal characteristics and model inputs for the higher and lower intake evaluation groups. Animal inputs Base High Base Low Dry matter intake, kg 24.5 22.84 Milk production, kg 41 36 Milk fat, % 3.7 3.7 Milk true protein, % 3.1 3.1 Milk lactose, % 4.78 4.78 Body condition score 3 3 Target body condition score 3 3 Age of first calving 22 22 Days in milk 11 11 Mature weight, kg 83 83 Age, months 39 39 Current weight, kg 75 75 Formulation values Total andfom and undf Rumen fill limits 1,65 lb cattle (75 kg) cattle 18 lb (8.2 kg) rumen andfom 1.8% BW Miner data suggested 1.28% BW andfom in rumen as maximum conditional on size of undf pool - consistent with the thumb rule of 1.2% BW andfom intake undf - 1.6 lb (4.8 kg) rumen undf - (.64% BW) Miner data.62% BW for undf limit Data from other rumen evacuations corroborates these values Model calculations related to rumen fill and passage 8,2 g maximum intake for andfom 4,8 g maximum intake for undf For 75 kg cow consuming 54 lb of DM: andfom kp = 1.6%/h Concentrate kp = 6.7%/h Liquid kp = 12.1%/h

Modeling comparisons using Rumen Fill for andfom and undf Standard diet Corn silage 35% DM 37% NDF High andfom corn silage variety 1% lower rumen fill Lower andfom corn silage variety 1% greater rumen fill Use model to estimate DMI at first limiting rumen fill set point or be within the limits of expected fill Formulation values Standard diet for this comparison 1,653 lb (75 kg) producing 9 lb (41 kg) milk consuming 54 lb (24.5 kg) DMI which is 32% andfom 17.28 lb andfom intake (7.84 kg) 7,84 g andfom intake 1 % body weight 217 Agronomic factors created higher undf making it difficult to meet typical corn silage intakes Chemical Composition CP (% DM) 16.3 SolP (% CP) 42.5 Ammonia (% SP) 7.9 ADIP (% CP) 6.5 NDIP (% CP) 15.8 NFC (% DM) 41.6 Sugars (% DM) 3.4 Starch (% DM) 29.9 31.8 pendf 58.4 Lignin (% NDF) 9.4 Ether extract (% DM) 4.4 Ash (% DM) 6.6 Forage (% DM) 6.3 Formulated Base Diet Chemical composition Fast pool andfom (% andfom) 55.4 Fast pool 17.6 Slow pool andfom (% andfom) 16.6 Slow pool 5.3 undfom pool (% andfom) 22.2 undfom pool (% DM) 7.1 Diet ingredient lb DM Inclusion level % Corn Silage Processed 35 DM 41 NDF Medium 22.46 42 Grass Silage 2 CP 48 NDF 5 LNDF 1.11 19 Corn Grain Ground Fine 9.17 17 Canola Meal Solvent 2.18 4 Corn Dist Ethanol.83 2 Soybean Hulls Ground 1.11 2 Citrus Pulp Dry 1.11 2 Wheat Midds 2.21 1 Soy Plus 1.96 4 Blood Meal Average.92 2 Energy Booster 1.37 1 MinVit +Urea + Smartamine M 1.55 3.1 Total 54. 1

Chemical composition Low andfom Base High andfom CP (% DM) 7. 7.5 8.1 37.7 37.3 37.8 Starch (% DM) 36. 37.1 32.1 undfom3 (% andfom) 47.8 45.1 41.4 undfom12 (% andfom) 38.6 34.7 29.8 undfom24 (% andfom) 36.7 32.6 27.7 Fast pool andfom (% andfom) 49.5 51.8 55.4 Slow pool andfom (% andfom) 13. 15. 16. undfom pool (% andfom) 36.7 32.6 27.7 Fast kd (%/h) 12.4 12.1 11.6 Slow kd (%/h) 1.8 1.8 1.8 Integrated kd (%/h) 6.3 5.9 5.9 undfom (% andfom) 1..9.8.7.6.5.4.3.2.1. In vitro andfom degradation curves High andfom Base Low andfom 3 6 9 12 15 18 21 24 Time (h) CNCPS v7 Meal pattern and fill/flux of DM Rumen DM (g) 7 6 5 4 3 2 1 High Base 276 281 286 291 296 CNCPS v7 Model Output Rumen Pool Sizes and DMI Low andfom Standard High andfom B3 Fast CHO 1588 1632 1698 B3 Slow CHO 1588 1655 1715 C CHO 5239 4819 (.64% BW) 4395 Total rumen NDF 8415 816 (1.1% BW) 789 DMI 24.5 24.5 24.5 Simulation time (hr)

Rumen Fill and Rumen Function The low will overfill thus intake drops The high will under fill thus opportunity for greater DMI What happens to the high diet when time budgets are off, feed availability is reduced, or inventories are tight? What if andfom was formulated at 28% DM instead of 3% CNCPS v7 Rumen Fill andfom at Equal DMI Rumen fill capacity CNCPS v7 Rumen Fill undfom DMI set to equal Fast Pool andfom at equal DMI Rumen fill capacity

Slow Pool andfom at equal DMI CNCPS v7 Model Output estimated DMI based on first limiting for fill andfomor undf Low andfom Standard andfom High andfom B3 Fast CHO, g 1464 1632 1763 B3 Slow CHO, g 1462 1655 178 C CHO, g 4819 4819 4563 Total rumen NDF, g 7745 816 816 DMI, kg 22.5 24.5 25.4 ME allowable 35.8 4.9 43.3 milk Corn silage evaluation This approach consistent with a nutritionist that has to substitute corn silage with other forage or non forage fiber sources to maintain milk yield when decreases or undf increases Or has the ability to feed more forage/dm when increases Currently does not account for yield or have economics integrated in the evaluation Summary This modeling approach allowed us to integrate some concepts of physical fill and describe digestion of andfom in the rumen and evaluate first limiting physical fill factors Suggests estimates of DMI can be evaluated at the time of diet formulation to determine the feasibility of application Further work will continue to develop datasets that allow us to challenge our understand of rumen fill and emptying to better predict potential differences in feed intake based on physical and chemical components of the diet to improve diet formulation capacity

Corn silage chemistry NDF fractions what is a good number? Thank you for your attention ~7% of andfom in the fast pool ~7% in the slow pool ~23% in the indigestible pool >6% by 3 hr mev1@cornell.edu