ASF epidemiological situation in Estonia update. PAFF meeting Brussels Estonian Veterinary and Food Board 1

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ASF epidemiological situation in Estonia update PAFF meeting Brussels 09.09.2015 Estonian Veterinary and Food Board 1

ASF in wild boars (1) Wild boar population Hunters estimation for the number of wild boar (2015):20 606 Compared to year 2014 the population in total has increased 4,5 % (decreased in Valga and Võru county) Wild boar density *Source of information: Estonian Environment Agency 2

ASF in wild boars (2) Start of the events First case of wild boar: On 2nd September 2014 one wild boar piglet was found dead in Valga district, Hummuli parish, 6 km from the Latvian border Samples taken: spleen, tonsils, kidneys Preliminary results from National Reference Laboratory (RT-PCR, ELISA) On 5th September confirmation from EU reference laboratory for African swine fever: RT-PCR positive, IPT positive ASFV belongs to genotype II Possible source of infection: movement of infected wild board from infected area (Latvia) 3

ASF in wild boars (3) Location of the first case Start of the events 4

ASF in wild boars (4) By the end of 2014 Positive cases and infected areas Location Number of positive wild boars Case no 1/Infected area 1 15 Case no 2/Infected area 2 47 Case no 3/Infected area 3 3 Case no 4/Infected area 4 1 Case no 5/Infected area 5 5 Case no 6/Infected area 6 1 Case no 7/Infected area 7 1 TOTAL 73 5

ASF in wild boars (5) By the end of 2014 7 infected areas had been established 6

ASF in wild boars (6) Infected areas 2015 7

ASF in wild boars (7) 2015 laboratory testing (04.09.2015) No of infected area/county No positive wild borars in 2014 No of positive wild boars in 2015 Total number of positive wild boars 1/Valga 12 27 39 2/Viljandi 47 125 172 3/Ida-Viru 3 19 22 (20 ELISA pos) 4/Valga 1 26 27 5/Võru 8 47 55 6/Võru 1 29 30 7/Ida-Viru 1 5 6 (4 ELISA pos) 8/Valga 33 33 9/Viljandi 8 8 10/Pärnu 5 5 11/Järva 7 7 12/Võru 17 17 13/Rapla 1 1 14/Pärnu 6 6 (1 ELISA pos) 15/Tartu 12 12 16/Põlva 12 12 17/Jõgeva 5 5 18/Põlva 2 2 19/Tartu 1 1 8

ASF in wild boars (8) Laboratory testing 2015 In total 3649 tested wild boars (04.09.2015) 440 found dead, 3206 hunted and 3 culled 387 positive wild boars (36 are only ELISA positive) 9

ASF in domestic pigs (1) 1st case (1) During 11th-12th July the owner (Valga county) noticed the continuous loss of appetite On 16th of July the owner notified authorized veterinarian that her pig has no appetite, is depressed and skin has turned red Veterinarian visited farm in the afternoon and carried out clinical examination: Loss of appetite, depression, hyperaemic skin Suspicion: bacterial infection Treatment: large scale antibiotics 10

ASF in domestic pigs (2) 1st case (2) By the morning of 18th of July the pig had died Authorized veterinarian informed animal health inspector in county veterinary centre who inspected the farm: Autopsy: abnormal enlargement of spleen Samples: blood, kidneys, spleen On 20.07 Veterinary and Food Laboratory confirmed ASF diagnose Measures taken by VFB: Restrictions on the farm, epidemiological investigation, notification Zoning and restrictions on the farms in the zones Destruction of the carcass in the rendering plant Cleaning and disinfection of the farm Possible source of the infection: human (berry/mushroom pickers)? 11

ASF in domestic pigs (3) 1st case (3) Location of the farm and zones 12

ASF in domestic pigs (4) 2nd and 3rd case (1) On 16th of July the owner noticed depression and loss of appetite in one of his pig farms, on the 18th of July he notices the same clinical signs in his other pig farm. Some pigs had already died In total 546 pigs were kept in two farms On 19th of July veterinarians visited both farms and carried out clinical examination and took samples: Blood, kidney, spleen On 20th of July Veterinary and Food Laboratory confirmed ASF diagnose Measures taken by VFB: Restrictions on the farm, epidemiological investigation Zoning and restrictions on the farms in the zones Destruction of the carcasses in the rendering plant Cleaning and disinfection of the farm 13

ASF in domestic pigs (4) 2nd and 3rd case (2) Location of the farms and zones 14

15

No of the outbreak ASF in domestic pigs (5) Current situation (1) Location Date of the confirmation No of culled pigs 1 Valga county 21.07.2015 1 2 Viljandi county 21.07.2015 355 3 Viljandi county 21.07.2015 191 4 Viljandi county 28.07.2015 6 5 Tartu county 30.07.2015 1186 + (145) 6 Järva county 30.07.2015 483 7 Valga county 31.07.2015 3 8 Lääne-Viru county 04.08.2015 2 9 Viljandi county 05.08.2015 2149 10 Jõgeva county 08.08.2015 6426 11 Võru county 12.08.2015 1868 12 Viljandi county 12.08.2015 3072 13 Valga county 19.08.2015 5 14 Jõgeva county 20.08.2015 2329 15 Tartu county 25.08.2015 3804 16 Valga county 25.08.2015 120 17 Viljandi county 29.08.2015 15 22015 + (145) 16 Total

ASF in domestic pigs (6) Current situation (2) Map with surveillance zones of 17 infected farms 17

ASF in domestic pigs (7) Laboratory testing Number of tested animals: 3521 172 positive animals 172 PCR positive 5 ELISA positive 18

ASF in domestic pigs (8) Culling, carcass disposal Culling methods Gassing, lethal injection, gun Carcass disposal Rendering plant (15 farms) Burial (2 farms) 10 000 pigs Capacity problems in rendering plant 19

Hunting, feeding According to the decree of Environmental Board (18.08.2015): Hunting bag of wild boar was increased to 29 600 wild boars (previously ca 20 000 wild boars) Additional feeding is not allowed during the period of 01.10.2015-30.04.2016 Small amounts allowed for baiting (max 100 kg per feeding slot + 10 kg of feed on the ground) during the still hunting Driven hunts are allowed 20

Rules for the control of the African swine fever and classical swine fever (Regulation of Ministry of Agriculture No 179 from 23.11.2004) (1) Latest issue came into force 01.09.2015 Additional biosecurity measures Pigs must be kept inside, territory of the farm is surrounded by fence Bedding must 90 before using be stored in a way that it will not be contaminated (wild boars, humans) Animal keeper must not bring any green fodder to the territory of the farm Uses heat-treated feed or feed that for 30 days has been stored in a way that it will not be contaminated (wild boars, humans) Biosecurity plan 21

Rules for the control of the African swine fever and classical swine fever (Regulation of Ministry of Agriculture No 179 from 23.11.2004) (2) Measures to be applied in the areas listed in Commission Implementing Decision 2014/709: Home slaughtering must be notified to Veterinary and Food Board 48h before slaughtering Hunters must notify about all shot/found dead wild boars to Veterinary and Food Board 22

Provisional conclusions Problems encountered Insufficient capacity of rendering plant Insufficient capacity of laboratory PCR equipment, preparation of samples Sending samples to laboratory Restrictions Animals, products Source of the infection? Green foddrer, berry/mushroom pickers, hunters?? 23

Estoniaś animal of the year 2015 (wise) wild boar Thank you for your attention! 24