MACS Marker Screen The performance of MACS Marker Screen was tested and compared with catalog reagents offered in single vials as liquid antibody reagents. The were reconstituted using 25 µl of deionized water each. Human peripheral blood mononuclear cells (PBMCs) were then added to each well and stained for 1 minutes at 4 C followed by two wash steps. The cells were then resuspended in 6 μl of PEB buffer, and analyzed using an MACSQuant Analyzer with an uptake volume of 25 µl. No significant differences in the staining patterns or median fluorescence intensity between the and liquid was observed. CD4 (VIT4) 25 CD11b 1 1¹ 1² CD4 (VIT4)-APC 1³ 25 1 1¹ 1² CD4 (VIT4)-APC 1³ CD2 25 25 25 25 CD14 1 1¹ 1² CD11b-APC 1³ 1 1¹ 1² CD11b-APC 1³ CD3 1 1¹ 1² CD2-APC 1³ 1 1¹ 1² CD2-APC 1³ 25 25 25 25 CD2 1 1¹ 1² CD14-APC 1³ 1 1¹ 1² CD14-APC 1³ 1 1¹ 1² CD3-APC 1³ 1 1¹ 1² CD3-APC 1³ 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD2-APC CD2-APC MACS Marker Screen May 216 1/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
CD21 CD45RA 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD21-APC CD21-APC CD45RA-APC CD45RA-APC CD22 CD45RO 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD22-APC CD22-APC CD45RO-APC CD45RO-APC CD38 CD56 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD38-APC CD38-APC CD56-APC CD56-APC CD41b CD57 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD41b-APC CD41b-APC CD57-APC CD57-APC CD45 CD64 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD45-APC CD45-APC CD64-APC CD64-APC MACS Marker Screen May 216 2/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
CD99 CD196 (CCR6) 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD99-APC CD99-APC CD196 (CCR6)-APC CD196 (CCR6)-APC CD122 (IL-2Rβ) CD197 (CCR7) 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD122 (IL-2Rβ)-APC CD122 (IL-2Rβ)-APC CD197 (CCR7)-APC CD197 (CCR7)-APC CD156c (ADAM1) CD222 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD156c (ADAM1)-APC CD156c (ADAM1)-APC CD222-APC CD222-APC CD161 CD226 (DNAM) 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD161-APC CD161-APC CD226 (DNAM)-APC CD226 (DNAM)-APC CD185 (CXCR5) CD234 (DARC) 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD185 (CXCR5)-APC CD185 (CXCR5)-APC CD234 (DARC)-APC CD234 (DARC)-APC MACS Marker Screen May 216 3/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
CD235a (Glycophorin-A) CD335 (NKp46) 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD244 (2B4) CD235a (Glycophorin A)- APC CD235a (Glycophorin A)- APC Anti-CLA CD335 (NKp46)-APC CD335 (NKp46)-APC 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD244 (2B4)-APC CD244 (2B4)-APC Anti-CLA-APC Anti-CLA-APC CD3e (IREM-2) Anti-CX3CR1 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD3e (IREM-2)-APC CD3e (IREM-2)-APC Anti-CX3CR1-APC Anti-CX3CR1-APC CD3f (IREM) Anti-HLA-DR 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD3f (IREM)-APC CD3f (IREM)-APC Anti-HLA-DR-APC Anti-HLA-DR-APC CD314 (NKG2D) Anti-HLA-DR, DP, DQ 25 25 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD314 (NKG2D)-APC CD314 (NKG2D)-APC Anti-HLA-DR, DP, DQ-APC Anti-HLA-DR, DP, DQ-APC MACS Marker Screen May 216 4/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
Anti-HLA-E 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ Anti-HLA-E-APC Anti-HLA-E-APC Anti-HLA-ABC 25 25 1 1¹ 1² Anti-HLA-ABC-APC 1³ 1 1¹ 1² Anti-HLA-ABC-APC 1³ Anti-PSA-NCAM 25 1 1¹ 1² Anti-PSA-NCAM-APC 1³ 25 1 1¹ 1² Anti-PSA-NCAM-APC 1³ Anti-TCRα/β 25 25 1 1¹ 1² 1³ 1 1¹ 1² 1³ Anti-TCRα/β-APC Anti-TCRα/β-APC Miltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 224 836- Fax +49 224 85197 macs@miltenyibiotec.de www.miltenyibiotec.com Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, and MACSQuant are registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright 216 Miltenyi Biotec GmbH. All rights reserved. MACS Marker Screen May 216 5/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.