Use of sheep horn hydrolysate as nitrogen source for lactic acid bacteria growth Ayat A A, Zaid A T, Hayder H I Biotechnology Research Center/ AlNahrain University ( SHH ) Sheep Horn Hydrolysate ( SHHM ) ( 138 131 ) Lactobacillus acidophilus nm 540 Lactobacillus SHHM ( 132 127 ) acidophillus Klebsiella sp ( 18, 13, 14 ) SHHM Candida sp Staphylococcus aureus Klebsiella sp ( 12,10,12 ) Candida sp Staphylococcus aureus Klebsiella sp ( 20, 14, 14 ) SHHM ( 16, 13, 13 ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp / Abstract This study included used the sheep horn Hydrolysate as a nitrogen source in growth medium (SHHM) for cultivation of lactic acid bacteria, The results showed the optical density of growth in 540 nm for and lactobacillus acidophillus was 138 and 131 respectively after cultivation in (SHHM) medium Otherwise the optical density of growth for and lactobacillus acidophillus was 127 and 132, respectively after cultivation in () medium, Which contain peptone, yeast extract and meat extract as a nitrogen source In the inhibition capability test the results showed that the inhibition zone average of cell free extract for lactobacillus casei which cultivated in SHHM was (14, 13 and 18) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively, while the inhibition zone average of cell free extract for lactobacillus casei which cultivated in was (12, 10 and 12 mm) against Klebsiella sp, Staphylococcus aureus and Candida sp Respectively The 79
inhibition zone average of cell free extract for lactobacillus acidophillus which cultivated in SHHM was (14, 14 and 20) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively, and ( 13, 13 and 16 ) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively after cultivated in medium 1 ],Glutamine ) Lactobacillus ( Fastidious ) Lactobacillus Lactobacillus [3] [ 2 ( Arginine,Leucine,Isoleucine, Valine, Phenyl alanine, Tryptophane Lactobacillus helveticus Lactobacillus [4] Lactobacillus plantarum acidophillus B12 [5] [6] [7] ( Prebiotic ) ) ( 6HCL No1 Sheep Horn [8] 4 Agar 15 % 1 2 3 ) ( Candida sp Staphylococcus aureus Klebsiella sp? 100 50 35 Horn Flour (HF) 100 24 80 30 400 ( / ) % 4 Hydrolysate (SHH) 1 (Himedea) Mueller Hinton 2 (Difico) 80
3 () Sodium acetate trihydrate 5 ( / ) % 4 MgSO47H2O 02 Triammonum citrate 2 3 Tween80 1 Dglucose 20 4 (SHHM2) MnSO47H2O 005 Dglucose 20 ( / ) % 5 ( / ) % 4 (SHHM3) 02 Triammonum citrate 2 Tween80 1 Sodium acetate trihydrate MnSO47H2O 005 MgSO47H2O 15 2 ( / 15 ) 121 [9] Cell Free Extract (CFE) 1 37 % 25 100 24 4 25 / 5000 µ m 045 [9] Well Assay 2 25 23 01 LShape 37 5 (1) CFE 005 24 37 [10] 540nm (2 1 ) 127 SHHM3 SHHM2 ( 109 11 131 ) ( 11 11 138 ) ( 132 ) %14, ( SHHM3 SHHM2 ) [8] % 22 [ 2 1 ] [11] Cysteine SHHM3 Keratin SHHM2 81
540nm 24 ( 1 ) 37 540nm 24 37 ( 2 ) ( 1 ) Klebsiella sp ( 18 13 14 ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp ( 12 10 12 ) ( 2 ) Klebsiella sp ( 20 14 14 ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp ( 16 13 13 ) Prebiotic ( 13 12 ) Probiotic 82
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