Using Tissue and Soil Tests Together Helps Make Better Decisions. John Lee Soil Scientist AGVISE Northwood, ND

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Using Tissue and Soil Tests Together Helps Make Better Decisions John Lee Soil Scientist AGVISE Northwood, ND

Winter Wheat Tissue Demo Northwood, ND Prevent Plant field from 2011 Winter wheat seeded late Start fertilizer only at seeding

October 31, 2011 Soil N - 15 lb/a 0-24

Winter Wheat Tissue Example 2012 Good and Bad Tissue Samples, Good and Bad Soil Samples Green Wheat N applied April 26 240 lb Urea with Agrotain (108 actual N) No rain 14 day Yellow Wheat No- N applied

Winter Wheat Tissue and Soil Example 2012 Good and Bad Tissue Samples Tissue and soil samples May10, 2012 Green Wheat Tillering Stage - Whole above ground plant sample Tissue Total N - 3.4% (3.8-5.5% is sufficient) Yellow Wheat Tillering Stage - Whole above ground Plant sample Tissue Total N - 2.2% (3.8-5.5% is sufficient)

Winter Wheat Tissue and Soil Example 2012 Good and Bad Soil Samples Tissue and soil samples May10, 2012 Green Wheat Soil Nitrate 9 lb/a Ammonium N 20 lb/a Total Soil N 29 lb/a (0-6 ) Yellow Wheat Soil Nitrate 1 lb/a Ammonium N 7 lb/a Total Soil N 8 lb/a (0-6 )

Winter Wheat Tissue and Soil Example 2012 Good and Bad Tissue Samples, Good and Bad Soil Samples Tissue and soil samples May10, 2012 Green Wheat Tillering Stage - Whole above ground plant sample Tissue Total N - 3.4% (3.8-5.5% is sufficient) Soil Nitrate 9 lb/a Ammonium N 20 lb/a Total Soil N 29 lb/a (0-6 ) Yellow Wheat Tillering Stage - Whole above ground Plant sample Tissue Total N - 2.2% (3.8-5.5% is sufficient) Soil Nitrate 1 lb/a Ammonium N 7 lb/a Total Soil N 8 lb/a (0-6 )

Winter Wheat Tissue Example 2012 Good and Bad Tissue Samples, Good and Bad Soil Samples Green Wheat May 21 (More N applied) 75 lb Urea - no Agrotain 35 lb actual N ½ Rain the next day Yield 55 bu Protein 14.6% Yellow Wheat No- N applied 20 bu yield estimate (small area) Protein 10.7%

Winter Wheat Tissue and Soil Test Demo Tissue test confirmed N was low in bad area and marginal in Good area Soil test confirmed bad area was very low in N and Good area may need more N applied N fertilizer made large difference in % protein and yield

Corn Tissue and Soil Test Demonstration - Starter

Plant size without and with starter Starter 5 gallons 10-34-0 with 2 pints of zinc chelate

No Starter With Starter

No Starter Tissue Total N 4.1% (3.5-5.0 is sufficient) Tissue Total P -.48% (.35-.80 is sufficient) Tissue Total Zn 25 ppm (20-75 is sufficient) With Starter (5 gallon 10-34-0-2pt Zn) Tissue Total N 5.6% (3.5-5.0 is sufficient) Tissue Total P -.83% (.35-.80 is sufficient) Tissue Total Zn 56 ppm (20-75 is sufficient) Tissue Test Results Picture 5-23-2012

No Starter Soil nitrate in row 16 lb/a (0-6 sample) P Soil test in row 15 ppm Zn Soil test in row 1.6 ppm With Starter (5 gallon 10-34-0-2pt Zn) Soil nitrate in row 102 lb/a (0-6 sample) P Soil test in row 68 ppm Zn Soil test in row 5.9 ppm Soil Test Results Picture 5-23-2012

No Starter Tissue Total N 4.1% (3.5-5.0 is sufficient) Soil nitrate in row 16 lb/a (0-6 sample) Tissue Total P -.48% (.35-.80 is sufficient) P Soil test in row 15 ppm Tissue Total Zn 25 ppm (20-75 is sufficient) Zn Soil test in row 1.6 ppm With Starter (5 gallon 10-34-0-2pt Zn) Tissue Total N 5.6% (3.5-5.0 is sufficient) Soil nitrate in row 102 lb/a (0-6 sample) Tissue Total P -.83% (.35-.80 is sufficient) P Soil test in row 68 ppm Tissue Total Zn 56 ppm (20-75 is sufficient) Zn Soil test in row 5.9 ppm Picture 5-23-2012

Tissue Plus Soil Test Helps Make better Decisions Tissue test showed differences between short and tall corn was starter Even though short and tall both showed sufficient Nutrient levels for N, P and Zn Soil test confirmed lack of starter was the difference Soil test N,P, Zn in the row for tall was much higher than soil test in row where no starter had been applied

Corn Tissue and Soil Test Nitrogen Demonstration Soil Test Levels Previous crop soybeans N - 25 lb/a P - 12 ppm (Olsen P) K 100 ppm S 30 lb/a Zn -.9 ppm OM 1.5% ph 6.2

Corn Nitrogen Demo Check area (no starter - no broadcast N,P,K,) N Top-dressed at 6 corn (ammonium sulfate) 50 and 100 lb/a actual N (about May 25) Grower Treatment (5 gallon 10-34-0 starter plus Zn) 110 N 59 P 2 O 5 60 K 2 0 10 S.5 Zn

Grower Treatment Starter, 110 N, 58 P, 60K 10 S July 17 picture 100 N no starter 50 N no starter Check

% Total N Corn Leaves Corn N Tissue Tests Check, 50, 100, 110 (Grower Rate) 4.50 4.00 3.50 3.00 Suff = 2.8-4.0% 2.50 2.00 1.50 Deficient <1.7 % 1.00 7/9/2012 7/17/2012 check 50N 100N 110 (GT)

N Deficiency showing on lower leaves, even on high N treatment

Corn Stalk Nitrate and Soil N Test at the end of the season (September 26) Treatment Corn Stalk Nitrate ppm Check 35 ppm 5 lb/a 50 lb/a N topdressed 185 ppm 8 lb/a 100 lb/a N topdressed 162 ppm 8 lb/a Grower trt 110 lb/a N pre-plant incorporated 188 ppm 24 lb/a Fall Soil Nitrate 0-24 lb/a Corn Stalk Nitrate Interpretation Low: (Less than 250 ppm) Likely that N deficiency limited yield Marginal: (250-700) Possible that N deficiency limited yield Optimal: (700-2000) Yield was not limited by N Excessive: (>2000 ppm) Nitrogen supply was excessive

Corn Tissue and Soil Test N Demonstration Tissue test identified where N was limiting Corn Stalk nitrate Test confirmed N was limiting (even with grower rate!) Soil Test after harvest was very low where N was limiting

Sugarbeet Soil and Tissue Demonstration Mid June - Sandy ridges yellowing off Warren, MN North Star Ag Service Scott Edgar

Sugarbeet Soil Analysis June 20 Site Appearance Sulfur 0-6 Sulfur 6-24 Chloride 0-6 Chloride 6-24 lb/a lb/a lb/a lb/a Yellow 8 24 4 8 Green 108 240+ 22 104 Sulfur Sufficiency Range: 15 lb/a topsoil? Chloride Sufficiency Range: 40 lb for wheat? Soil nitrate for good and bad >80 lb/a 0-24

Tissue Analysis - June 27 Site Sulfur Chloride Yellow.14%.22% Green.23%.87% Sulfur Sufficiency Range:.25% to.50% Chloride Sufficiency Range: Sugarbeets? All other nutrients in sufficiency range

Sugarbeet Tissue Analysis After Fertilizer Trial Strips (July 11) Treatment Sulfur Chloride Yellow (check).13%.13% Green (adjacent good).28%.41% Potassium Chloride 50 lb/a.17%.42% AMS - 50 lb/a Potassium Chloride 50 lb.50%.47%

Tissue and soil tests helped to identify what was Different between the green and yellow beets Research project planned by U of M/Crystal Sugar Starting next year to address this issue North Star Ag Service Scott Edgar

Corn Soil and Tissue Demonstration Purple and Stunted plants Previous Crop was Sugarbeets Low rate of low P Content starter fertilizer with seed Greg Reidman Riverbend Agronomy

Corn Purple and Stunted Soil Test May 24 P 21 ppm (Olsen) K 241 ppm S 16 lb OM 4.0% Salt 0.8 mmhos/cm ph 7.7

Sugarbeets don t support Mycorrhizae (Fallow syndrome) Fungus helps plant take up nutrients like P and Zn Previous crop of sugarbeets, canola, fallow Don t support mycorrhizae and fungus pop decreases Following crop needs extra P fertilizer Corn needs high starter rate and extra P broadcast

Tissue Analysis - May 24 Phosphorus very low in tissue.17% Sufficiency range is.35% to.8% P soil test 21 ppm (very high)

P Def. - Rescue in season? 6 gallon 9-24-3 applied placed near row with single disc 4 gallon 10-34-0 + Zn Foliar application

P Def. - Rescue in season? Tissue Test 2 weeks trt Check -.24% P Treated -.22% P (Sufficiency Range is.35% -.80%) No difference in growth No difference in tissue P Yield loss estimated at 40 bu/a

Corn Tissue and Soil Test Demo Purple Corn Tissue Test indicated low P was issue Soil Test was high in P (Low rate P starter) Previous Crop of Sugarbeets pointed towards Fallow Syndrome Rescue Treatment did not work Strong Starter P program for corn following sugarbeets or do not plant corn after beets

Navy Bean Tissue and Soil Test Demonstration Fertilized and unfertilized

Navy Bean Soil Test July 17 Demonstration - Hatton ND Treatment N 0-6 P (Olsen) K S 0-6 Zn OM Lb/a ppm ppm lb/a ppm % check 10 30 235 6 2.6 2.6 Fertilized 24 24 227 14 2.4 2.8 20-31-0-15 broadcast and tilled in before planting

% Total P Bean Tissue P Tissue levels Navy Beans 0.6 0.5 0.4 P Sufficiency range = 0.25%- 0.50% 0.3 0.2 0.1 6/21 7/5 7/12 7/26 8/2 check Fertilized Olsen P soil test = 20-30 ppm (High)

% Total K Bean Tissue K Tissue levels Navy Beans 4.00 3.50 3.00 2.50 2.00 Sufficiency range = 1.8%-2.5% 1.50 1.00 0.50 0.00 6/21 7/5 7/12 7/26 8/2 check fertilized K soil test = 235 ppm

Navy Bean Tissue and Soil Test Demonstration Tissue levels for P & K dropped off once topsoil got dry Soil test showed both P and K levels were high Both fertilized and unfertilized area had uptake issues once topsoil got dry As crop approached maturity we expect most nutrient levels to decline, but not this early! Tissue test does not account for issues like dry soil

Tissue and Soil Tests (Good and Bad) Provide Better Information Very dry areas in 2012: Many tissue samples showed nutrient levels dropping off rapidly as topsoil dried out Tissue testing alone may indicate nutrients are low, while a soil test shows the nutrient level to be sufficient or high. Reduced nutrient levels in plants also happen with excessive water, compaction etc. Having both soil and tissue tests to compare Good and Bad areas will help make better decisions!

Tissue Test and Soil Test (Good and Bad) Provides Better Information

Questions

Corn Soil and Plant demo Purple corn field with one Green strip Tara King Southern Manitoba

Corn Soil and Tissue Demonstration Purple and Stunted plants Previous Crop - canola No starter fertilizer with seed Fallow syndrome suspected

Fallow syndrome is a situation where a beneficial fungus called Mycorrhizia die because there are no host plant roots to grow on. Mycorrhizia is a beneficial fungus which helps plants take up phosphorus, zinc etc. Crops following fallow need extra P fertilizer. Canola and sugarbeets do not support Mycorrhizia fungus so they die off. The following crop then needs extra P fertilizer to ensure adequate P uptake.

Purple stunted corn Previous crop: canola Tissue P =.14% (suff.35 -.80) P soil test 8 ppm (Olsen) Green normal height corn Previous crop: Old tree row Removed previous year Tissue P =.28% (suff.35 -.80) P soil test 8 ppm (Olsen)