Supplementary Table 1. Association of rs3826795 with risk of obesity among participants in NHS and HPFS Case/control NHS (1990) HPFS (1996) OR (95% CI) P- value Case/control OR (95% CI) P- value Obesity 1503/3789 1.04 (0.90,1.19) 0.61 829/2204 0.86 (0.70,1.07) 0.18 Obesity (30) 1503/6117 1.04 (0.92,1.18) 0.51 829/4926 0.87 (0.72,1.05) 0.14 Overweight 3831/3789 0.97 (0.88,1.06) 0.45 3551/2204 1.04 (0.93,1.17) 0.48 Obesity was defined as BMI 30 and participants with BMI<25 served as the reference group. Obesity (30) was defined as BMI 30 and participants with BMI<25 served as the reference group. Overweight was defined as BMI 25, and participants with BMI<25 served as the reference group The logistical regression model was used to test the association of SNP with risk of obesity after adjustment of age, source of genotyping data, smoking, alcohol intake, physical activity, total energy intake, television watching, alternate healthy eating index.
Supplementary Table 2. Stratified analyses of association of rs3826795 with 10 years changes in BMI among participants in NHS (1980 ~ 1990) and HPFS (1986 ~ 1996) NHS (1980 ~ 1990) HPFS (1986 ~ 1996) Beta ± SE P P-interaction Beta ± SE P P-interaction Age, year 0.35 0.84 <50-0.05±0.21 0.82-0.22±0.32 0.49 50 0.20±0.21 0.33-0.11±0.17 0.54 Alcohol intake, gram/day 0.40 0.09 Low (<10) 0.11±0.18 0.53-0.31±0.20 0.13 High ( 10) -0.21±0.26 0.42 0.22±0.24 0.36 Smoking status 0.03 0.46 Never smoke 0.34±0.22 0.12-0.23±0.21 0.28 Ever Smoke -0.04±0.26 0.89 0.00±0.22 0.99 Physical activity, MET-hr/wk 0.76 0.10 Low (<14) 0.01±0.19 0.95-0.33±0.19 0.09 High ( 14) 0.16±0.24 0.52 0.28±0.26 0.29 SE: standard error: MET-hr/wk: metabolic hours per week Data on lifestyle and dietary intake were assessed in 1980 (NHS) and 1986 (HPFS). The linear regression model was used to test the association between rs3826795 and BMI after adjustment of age, source of genotyping data, smoking, alcohol intake, physical activity, total energy intake, television watching, alternate healthy eating index.
Supplementary Table 3. Association of baseline total B vitamins intake with 10 years changes in BMI (kg/m 2 ) among participants in NHS (1980~1990) and HPFS (1986~1996) Cohorts Tertiles of total B vitamins intake * T1 T2 T3 P-for trend VitaminB 2, mg NHS 1.30±0.05 1.29±0.05 1.37±0.07 0.49 HPFS 0.49±0.04 0.58±0.04 0.61±0.04 0.04 Pooled 0.81±0.75 0.86±0.80 0.80±0.73 0.09 VitaminB 6, mg NHS 1.24±0.06 1.31±0.05 1.43±0.06 0.03 HPFS 0.51±0.04 0.56±0.04 0.62±0.04 0.07 Pooled 0.74±0.67 0.85±0.79 0.87±0.80 0.02 Folate, mcg NHS 1.33±0.06 1.26±0.06 1.34±0.06 0.84 HPFS 0.54±0.04 0.55±0.04 0.59±0.04 0.46 Pooled 0.78±0.72 0.77±0.70 0.82±0.76 0.75 VitaminB 12, mcg NHS 1.24±0.05 1.30±0.06 1.41±0.06 0.03 HPFS 0.42±0.04 0.62±0.04 0.65±0.04 <0.0001 Pooled 0.74±0.68 0.83±0.76 0.88±0.82 <0.0001 * Plus-minus values are means ±SD. mg: milligrams; mcg: micrograms NHS: Nurses Health Study, HPFS: Heath Professionals Follow-up Study Data on dietary intake were assessed in 1980 (NHS) and 1986 (HPFS). Data on BMI were assessed in 1980 (NHS) and 1990 (NHS) and 1986 (HPFS) and 1996 (HPFS). The general linear model was used to test the association of baseline total B vitamins intake with 10 years changes in BMI after adjustment for age, source of genotyping data, smoking, alcohol intake, physical activity, total energy intake, alternate healthy eating index, television watching, baseline BMI, and other B vitamins (mutually adjusted). Results for the two cohorts were pooled by means of inverse-variance-weighted, fixed-effects meta-analyses.
Supplementary Table 4. Differences in 10 years changes in BMI per minor allele of rs3826795, according to baseline B vitamins intake from food among participants in NHS (1980~1990) and HPFS (1986~1996) Cohorts $ Tertiles of B vitamins from food * T1 T2 T3 P-interaction VitaminB 2, mg NHS 0.07±0.10-0.03±0.09 0.11±0.10 0.91 HPFS -0.14±0.07 0.07±0.07-0.06±0.07 0.64 Pooled # -0.07±0.06 0.03±0.06 0.00±0.06 0.89 VitaminB 6, mg NHS 0.14±0.10-0.04±0.09 0.05±0.11 0.82 HPFS -0.09±0.07-0.10±0.07 0.04±0.07 0.39 Pooled -0.01±0.06-0.08±0.06 0.04±0.06 0.69 Folate, mcg NHS 0.08±0.10 0.09±0.10-0.05±0.10 0.55 HPFS -0.08±0.07-0.09±0.07 0.00±0.07 0.24 Pooled -0.03±0.06-0.03±0.06-0.02±0.06 0.4 VitaminB 12, mcg NHS 0.01±0.10-0.01±0.10 0.12±0.10 0.14 HPFS -0.09±0.06 0.09±0.07-0.14±0.07 0.08 Pooled -0.06±0.05 0.06±0.06-0.05±0.06 0.06 * Plus-minus values are β coefficients ±SE. Data on dietary intake were assessed in 1980 (NHS) and 1986 (HPFS). Data on BMI were assessed in 1980 and 1990 in NHS and 1986 and 1996 in HPFS. $ The general linear model was used to test the genetic association of baseline B vitamins intake from food with 10 years changes in BMI after adjustment for age, source of genotyping data, smoking, alcohol intake, physical activity, total energy intake, alternate healthy eating index, television watching, baseline BMI, and other B vitamins from food (mutually adjusted). # Results for the two cohorts were pooled by means of inverse-variance-weighted, fixed-effects meta-analyses.
Supplementary Table 5. The amount of baseline B vitamins in corresponding tertiles in NHS and HPFS Cohorts Tertiles of B vitamin intake * T1 T2 T3 NHS Total vitamins intake Riboflavin (B 2 ), milligrams/day 1.4 2.3 6.6 Folate, micrograms/day 186.5 325.6 649.9 Pyridoxine (B 6 ), milligrams/day 1.3 2 5.3 Vitamin B 12, micrograms/day 4 7.9 16.4 Supplemental vitamins Riboflavin (B 2 ), milligrams/day 0 0.4 6.2 Folate, micrograms/day 0.1 1.5 476.2 Pyridoxine (B 6 ), milligrams/day 0 0.3 4.9 Vitamin B 12, micrograms/day 0.2 1.5 11.5 Food sourced vitamins Riboflavin (B 2 ), milligrams/day 1.2 1.6 2.3 Folate, micrograms/day 167.9 245.4 377.1 Pyridoxine (B 6 ), milligrams/day 1.1 1.5 2.2 Vitamin B 12, micrograms/day 3.5 5.3 9.2 HPFS Total vitamins intake Riboflavin (B 2 ), milligrams/day 1.6 2.5 11.4 Folate, micrograms/day 265.9 397.7 763 Pyridoxine (B 6 ), milligrams/day 1.8 2.7 20.9 Vitamin B 12, micrograms/day 5.6 9.7 22.5 Supplemental vitamins Riboflavin (B 2 ), milligrams/day 0.1 1 12.7 Folate, micrograms/day 5 66.3 497.9 Pyridoxine (B 6 ), milligrams/day 0.1 1.1 25.1 Vitamin B 12, micrograms/day 0.2 1.7 13.9 Food sourced vitamins Riboflavin (B 2 ), milligrams/day 1.5 1.9 2.5 Folate, micrograms/day 248.3 337.2 470.1 Pyridoxine (B 6 ), milligrams/day 1.7 2.1 2.8 Vitamin B 12, micrograms/day 5 7.7 14.1 Data was presented as median