VMC-221: Veterinary Immunology and Serology (1+1) Objective type Questions Question Bank Q. No. 1 - Fill up the blanks with correct words 1. The British physician, who developed the first vaccine against an infectious disease is 2. The two effector molecules of specific immune response are & 3. The cells that respond first to an antigen is 4. The antibodies that are attached to the surface of B cells are referred as 5. The differentiation markers of T lymphocytes are 6. Extracellular phagocytosis is performed against parasites by 7. The high affinity binding CD receptor for Fc portion of antibody is 8. The small antigens that cannot stimulate the production of antibodies are 9. The antibody isotype that is more prevalent in anamnestic response is 10. The TLR that binds with the LPS of bacteria is 11. The protein of MHC II, that prevents the binding of other protein at the cleft region at rough ER is 12. The TCR-CD3-zeta chain complex is responsible for (function) 13. The communication molecules of immune system are 14. The receptor that strengthens the bond between TCR and MHC II is 15. The effector in delayed type hypersensitivity of cell mediated immune response is 16. The lymphocytes that mediate ADCC is 17. The classical complement pathway starts with the activation of 18. The immediate allergy with systemic response is &
Q. No. 2 - State whether the statements are true of false, if false, correct them. No. 1 Statement The development of granuloma in Type IV hypersensitivity is due to the inability of the pre CTL to convert into active CTL 2 Injection of allogenic immunoglobulins are responsible for serum sickness (Type III hypersensitivity) in animals 3 The alternate complement pathway contributes to the specific immune response 4 Interaction between Th1 cell and B cell results in the proliferation and differentiation of B cell and production of antibodies 5 The non-specific killing of cells having endogenous antigen is mediated through macrophages 6 The only mechanisms by which activated CTL performs lethal hit is through osmolysis 7 High affinity IgM and IgG are produced by plasma cells activated by carbohydrate antigens 8 The term epitope is associated with both T dependant and T independent antigens 9 Specific immunity is produced as a result of interaction of T cells and B cells alone and no other cell is involved 10 Professional antigen presenting cells are not associated with the processing of endogenous antigens 11 Only epitopes presented by MHC alone can stimulate Abs or CTL 12 The hypohalide compounds formed inside the phagocytic cells is responsible for the destruction of foreign substances 13 The terms, Pattern recognition receptors (PRR) Pathogen associated molecular patterns (PAMP) are associated with scavenging receptors
14 GALT, MALT, BALT and NALT are organized primary lymphoid tissues 15 The binding of CD40 of APC with CD40L of helper T cell facilitates conversion of pre CTL to activated CTL 16 Macrophages have a single function of doing multiple phagocytosis and remain as matured cells in the blood circulation 17 Immunoglobulin family comprises of different isotypes and allotypes of antibodies alone. 18 The flexibility of certain isotypes of antibodies is due to the interaction of framework region in variable and constant domains 19 IgA exist as monomer in local secretion and dimer in serum 20 Opsonization is an efficient process of phagocytosis Q. No. 3-1 - Match the following 1 Louis Pasteur A Complement 1 2 Memory cells B Dendritic cells 2 3 Th1 cells C IgE 3 4 Th2 cells D ADCC 4 5 Macrophages E Birbeck granules 5 6 Dendritic cells F Garbage collectors 6 7 NK cells G B cell activation 7 8 Mast cells H Inflammation 8 9 Professional APC J bcl 2 9 10 Jules Bordet K Rabies vaccine 10 Q. No. 3-2 - Match the following 1 Signal transduction A Lytic pathway 1 2 Antibody B Long hinge region 2 3 C3b C Type II hypersensitivity 3 4 Helper T cells D Fas receptor 4 5 Cytotoxic T cells E IL 2 5 6 MAC F CD8 6
7 Apoptosis G CD4 7 8 Autoimmunity H CD35 8 9 IgD J CD64 9 10 Cytokine K CD3 10 Q. No. 4 - Expand the following 1 Ab 2 Ag 3 MAC 4 Ig 5 TLR 6 ADCC 7 APC 8 CTL 9 CD 10 GALT 11 TCR 12 BCR 13 BoLA 14 IL 15 CLIP 16 PMNL 17 CDR 18 Fc and Fab 19 ELISA
20 MALT Subjective type Questions Q. No. 1 - Differentiate / Explain the following 1. CDR and Frame work regions 2. What is the basis for allergic reactions? 3. What will happen when the complement system fails to get activated? 4. Why there is a T cell repertoire and why a single T cell is having 10,000-20,000 TCR? 5. Why substances like nylon, metal pins and flagellin are poorly antigenic? 6. What is the principle behind cross reaction involving Forssman antigen and heterophile antibodies? 7. Why there is strong inflammatory response during tuberculin testing in animals already exposed to tuberculosis? 8. What is the advantage of Fc portion of antibodies having same amino acids irrespective of differences at the Fab? 9. Why every time a bacterial carbohydrate antigen is treated as naïve antigen unlike protein antigen? 10. How TLRs contribute in innate immune response? 11. What is the disadvantage in giving xenogenic immunoglobulins against an acute disease? 12. What are the two primary roles of the thymus? 13. What effect would removal of the bursa of Fabricius (bursectomy) have on chickens? 14. Name the different types of immunoglobulins 15. Name the antibody that is involved in allergy. 16. Name different types of mucosa associated lymphoid tissue 17. Name different pathways of complement system. 18. What is the function of memory cells? 19. Which is largest secondary lymphoid organ? 20. Where are peyer s patches located?
Q. No. 2 - Write briefly on the following 1. Differentiate the methods of exogenous and endogenous antigen processing. 2. Draw a flow chart of sequence of action during respiratory burst? 3. List the products of C3, C4 and C5 and their function 4. Memory cells 5. List the three mechanisms of CMI and briefly explain ADCC 6. Name three features of a secondary immune response that distinguish it from a primary immune response 7. Name the primary and secondary lymphoid organs. 8. Describe the functions of various secondary lymphoid organs. 9. Compare antigen with immunogen. 10. Differentiate between TCR and BCR. 11. What are monoclonal antibodies? 12. Write down the functions of complement. 13. Name three major events of inflammation 14. Differentiate between systemic and localized infection 15. Differentiate between agglutination and precipitation. 16. Differentiate between primary follicle and secondary follicle 17. Differentiate between red pulp and white pulp. 18. Differentiate between stem cell and haematopoitic stem cell 19. What are three subpopulations of lymphocytes? 20. Write two characteristic features of mature B-lymphocytes Q.No. 3 - Write short answers on the following 1. Explain the sequence of reactions as a result of complement activation by free antigens with a neat flow diagram (or) sequence of reactions as a result of complement activation by antigen-antibody complex with a neat flow diagram. 2. Role of physical barriers in innate immune response. 3. Cytokines 4. What is immunity? How it differs from immune response? 5. Differentiate between lysozome and phagosome.
6. Differentiate between innate and acquired immunity. 7. What are the major types of cells in addition to T- lymphocytes which make up the frame work of the thymus? 8. Where the lymph nodes are located and what function they perform? 9. What are the reasons for the occurrence of autoimmune disorders? 10. Write a short note on Antigen presenting cells. 11. Role of sectretory immunoglobulins 12. Assessment of immunity and protection status of birds against Newcastle disease 13. Difference between live and inactivated veterinary vaccines 14. Difference between Anaphylaxix and Prophylaxix 15. Define primary and secondary binding immunoassays with examples 16. Difference between vaccination and variolation Q. No. 4 - Write long answers 1. (a) Differentiate B and T lymphocytes based on their morphology, functions, receptors and products (b) Differentiate the mechanism of phagocytosis between neutrophils, eosinophils and macrophages (c) How mast cells de-granulate and what are its impacts? 2. (a) Differentiate the structural and functional features of isotypes of antibodies (b) Differentiate innate and specific immune responses based on cells involved, products and mechanisms 3. (a) How antibodies are produced against protein and non-protein antigens? (b) List the different theories of antibody production and explain clonal proliferation theory 4. (a) Write in detail the different classifications of antigen (b) Explain the primary structure of antibody 5. What is hybridoma technology? Write different steps of monoclonal antibody production and application of monoclonal antibody in animal health. 6. Enumerate antigen antibody reactions. Describe the principle methodology and
clinical applications of agglutination reactions. 7. What is meant by immunity and explain about the various components involved in the immune system. 8. Define antibody and describe about their structure in detail with a neat diagram? 9. Explain the principle and applications of Radial and double immunodiffusion 10. Discuss the various components of Innate immune response. 11. Classify and define hypersensitivity. Describe type I I hypersensitivity.