COUNTRY REPORT ON MAP GENETIC RESOURCES SLOVENIA (ULBF, IHPS)

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COUNTRY REPORT ON MAP GENETIC RESOURCES SLOVENIA (ULBF, IHPS). Prof. dr. Dea Baricevic, University of Ljubljana, Biotechnical Faculty Msc.ag. Natasa Ferant, IHPS Žalec BRAGA, April 27th 2017

Monitoring and inventory of plant species for food and agriculture began in the 50s and 60s of the last century primarily for the purpose of breeding. Since 1996, plant genetic resources in Slovenia systematically collected, stored and maintained within the framework of the Slovenian plant gene bank (SPGB). Institutions involved: Agricultural Institute of Slovenia collection: 3220 accessions (Vegetables: 1466, Forage crops: 1033, Potato: 44, Cereals: 108, Small fruit: 169, Grape vine: 90, Fruit trees: 310); University of Ljubljana, Biotechnical Faculty (ULBF) collection: 1631 accessions (Cereals: 471, Maize: 614, Forage crops: 228, Fruit trees: 173, Medicinal and aromatic plants: 145); Institute of Hops research and Brewery of Slovenia (IHPS) collection: 262 accessions (Hops: 178, Medicinal and aromatic plants: 84 (97); University of Maribor, Faculty for Agriculture and Life Sciences (FKBV): 327 accessions (Fruit trees: 197, Grape vine: 130).

Ministry of agriculture, forestry and food of Slovenia: program of Slovene Plant Genebank (SPGB), a part of which MAP Genebank (from 1996 on). Curators (ULBF, IHPS) reponsible for study, characterization and evaluation of natural habitats and autochthonous MAP populations, using ECPGR descriptors (Environment and site, Crop specific descriptors ), multiplication of accessions, conservation in situ, ex situ and in vitro, data input in SPGB information system (IS) (from 2009 on).

Autochtonous populations of MAPs under inspection/control (in situ) on 51 natural habitats Phytocoenologically and edaphically evaluated 51 natural habitats 85 plant species, 244 MAP genetic resources (147 ULBF, 97 IHPS) medium term conserved (ex situ seeds in cold room/ refrigerator, plantation, in vitro - 5 accessions, in situ).

55 MAP populations: characterized/evaluated either from morphological, chemical, use, agronomic or (rarely) molecular aspect

Multiplication of existing accessions (short- term germinability and crosspollination of majority MAPs): in obtaining seed material of authentic population an isolation and controled pollination needed). Access ion Populatio n 1 9/3 Origanum vulgare L. subsp. vulgare 2 9/4 Origanum vulgare L. subsp. vulgare 3 9/6 Origanum vulgare L. subsp. vulgare Latin name Percentage of germination (%) 39 1,25 1 Surveying and field investigations of new natural habitats needed, acquering a new seed material, multiplication and evaluation of accessions in ecologic conditions of cultivation areas. 4 9/11 Origanum vulgare L. subsp. Vulgare 5 9/1 Origanum vulgare L. subsp. Hirtum 6 9/8 Origanum vulgare L. subsp. Vulgare 7 9/2 Origanum vulgare L. subsp. prismaticum (Gaudin) Arcang 68 68 26 63 Selection, development of cultivars, adapted to slovene agricultural conditions. 8 9/7 Origanum vulgare L. subsp. vulgare 9 9/10 Origanum vulgare L. subsp. vulgare 61 53

Status of MAP collections in SLOVENIA ULBF AND IHPS (2017): Achillea spp. 14 accessions, Artemisia spp. 6, Carum carvi 4, Gentiana lutea 7, Hypericum spp. 20, Melissa officinalis 1, Mentha spp. 1, Origanum spp. 13, Salvia officinalis 3, Thymus spp. 21 Taxon Accessions in total From own country ex-situ seed bank Achillea millefolium BF 9 9 9 Achillea millefolium 5 5 5 Acorus calamus 10 10 0 Agrimonia eupatoria 1 1 1 Alchemilla vulgaris BF 2 2 2 Alchemilla spp. 5 5 3 Allium carinatum BF 1 1 1 Allium rotundum BF 1 1 1 Anthemis nobilis BF 1 1 1 Anthyllis vulneraria BF 3 3 3 Anthyllis vulneraria 1 1 1 Arctium lappa 1 1 1 Arnica montana BF 3 3 0 Arnica montana 4 4 0 Artemisia absinthium BF 5 5 5 Artemisia nitida BF 1 1 1 Atropa belladonna BF 2 2 2 Betonica alopecorus BF 1 1 1 Betonica officinalis BF 2 2 2 Betonica officinalis 2 2 2 ULBF IHPS

Status of MAP collections in SLOVENIA ULBF AND IHPS (2017): Achillea spp. 14 accessions, Artemisia spp. 6, Carum carvi 4, Gentiana lutea 7, Hypericum spp. 20, Melissa officinalis 1, Mentha spp. 1, Origanum spp. 13, Salvia officinalis 3, Thymus spp. 21 Taxon Accessions in total From own country ex-situ seed bank Carum carvi BF 3 3 3 Carum carvi 1 1 1 Centaurea cyanus 1 1 1 Centaurium erythraea BF 2 2 2 Centaurium erythraea 1 1 1 Chelidonium majus BF 2 2 2 Cichorium intybus 1 1 1 Colchicum autumnale 1 1 1 Crataegus monogyna BF 1 1 1 Crataegus spp. 1 1 0 Cynara scolymus BF selection 1 1 1 Epilobium palustre BF 1 1 1 Epilobium parviflorum 4 4 4 Eupatorium cannabinum BF 1 1 1 Eupatorium cannabinum 1 1 1 Foeniculum vulgare BF 1 1 1 Fragaria vesca 1 1 1 Galium mugo 1 1 1 Galium odoratum 1 1 1 Galium verum BF 2 2 0 Gentiana lutea BF 6 6 6 Gentiana lutea 1 1 1 Geranium robertianum 1 1 1 ULBF IHPS

Status of MAP collections in SLOVENIA ULBF AND IHPS (2017): Achillea spp. 14 accessions, Artemisia spp. 6, Carum carvi 4, Gentiana lutea 7, Hypericum spp. 20, Melissa officinalis 1, Mentha spp. 1, Origanum spp. 13, Salvia officinalis 3, Thymus spp. 21 Taxon Accessions in total From own country ex-situ seed bank Herniaria glabra 1 1 1 Hypericum maculatum BF 1 1 1 Hypericum perforatum BF 9 9 9 Hypericum perforatum 10 10 10 Hyssopus officinalis 1 1 1 Inula hirta BF 2 2 2 Juniprus communis BF 1 1 1 Lavandula angustifolia BF 1 1 1 Linaria vulgaris 1 1 1 Lythrum salicaria 1 1 1 Malva silvestris 1 1 1 Melissa officinalis BF 1 1 1 Mentha longifolia BF 1 1 1 Ononis spinosa 1 1 1 Origanum vulgare ssp.vulgare BF 11 11 7 Origanum vulgare ssp.vulgare 2 2 2 Papaver bracteatum BF - selection LEK 1 1 1 Pimpinella saxifraga BF 2 2 2 Plantago lanceolata BF 1 1 1 Plantago lanceolata 3 3 3 ULBF IHPS

Taxon Accessions in total From own country ex-situ seed bank Plantago major BF 1 1 1 Plantago major 1 1 1 Plantago media BF 4 4 4 Polygonum bistorta 1 1 1 Potentilla anserina BF 1 1 1 Potentilla erecta BF 1 1 1 Potentilla erecta 4 4 4 Pulmonaria officinalis 1 1 1 Rhamnus catharcticus BF 1 1 1 Rhamnus frangula 1 1 1 Rosa canina BF 1 1 1 Rosa canina 1 1 0 Rosa dumalis BF 1 1 1 Rosa glauca BF 1 1 1 Rubia peregrina BF 1 1 1 Ruta divaricata 5 5 5 Ruta graveolens 1 1 1 Salvia officinalis BF 6 3 3 Salvia pratensis BF 1 1 1 Salvia verticillata BF 1 1 1 ULBF IHPS Status of MAP collections in SLOVENIA ULBF AND IHPS (2017): Achillea spp. 14 accessions, Artemisia spp. 6, Carum carvi 4, Gentiana lutea 7, Hypericum spp. 20, Melissa officinalis 1, Mentha spp. 1, Origanum spp. 13, Salvia officinalis 3, Thymus spp. 21

Taxon Accessions in total From own country ex-situ seed bank Sambucus nigra BF 1 1 1 Sanguisorba officinalis BF 1 1 1 Sanguisorba officinalis 1 1 1 Saponaria officinalis BF 1 1 1 Satureja montana BF 14 14 14 Satureja montana 1 1 1 Solidago virgaurea 2 2 2 Spartium junceum BF 1 1 1 Tanacetum vulgare 1 1 1 Taraxacum officinalis 1 1 1 Teucrium chamaedrys BF 3 3 3 Teucrium chamaedrys 1 1 1 Teucrium montanum 1 1 1 Thymus pulegioides BF 2 2 2 Thymus spp. BF 12 12 12 Thymus spp. 6 6 6 Thymus vulgaris selection BF 1 1 1 Tussilago farfara 1 1 1 Valeriana officinalis 4 4 4 Verbascum densiflorum 1 1 1 Verbena officinalis BF 1 1 1 Veronica chamaedrys BF 1 1 1 Veronica officinalis 1 1 1 ULBF IHPS Status of MAP collections in SLOVENIA ULBF AND IHPS (2017): Achillea spp. 14 accessions, Artemisia spp. 6, Carum carvi 4, Gentiana lutea 7, Hypericum spp. 20, Melissa officinalis 1, Mentha spp. 1, Origanum spp. 13, Salvia officinalis 3, Thymus spp. 21

Thank you!