Complex Plant Formulation of BIORAN Infla-Care

BIORAN Infla-Care (Magnolia Kobus Flower Extract, Chrysanthemum Indicum Flower Extract, Rosmarinus Officinalis Extract, Polygonum Cuspidatum Root Extract. Hamamelis Virginiana (Witch Hazel) Extract)

Complex Plant Formulation of BIORAN Infla-Care Magnolia Basic Information INCI INCI Magnolia Kobus Flower Extract Magnolia Kobus Flower Extract Chrysanthemum Indicum Indicum Flower Flower Extract Extract Rosmarinus Officinalis extract Rosmarinus Officinalis extract Polygonum Cuspidatum Root Extract 주효능효과 Hamamelis Virginiana (Witch Hazel) 항염 Extract Main 항산화 effect Anti-inflammation, Anti-irritation, and Anti-oxidation, and soothing Chrysanthemum Reynoutria Antiinflammation, Antioxidation Rosemary Witch Hazel

BIORAN Infla-Care (Anti-inflammation) Product Explanation Magnolia Magnolol and Honokiol, which are main ingredients, are proved to have anti-inflammatory and anti-microbial effect. Chrysanthemum Chrysanthemum performs anti-microbial, anti-allergy, and anti-viral action. Rosemary Rosemary is a compound word of Rosmary has Ros(dew) + Marinus(sea), meaning dew of sea. It has great anti-microbial and sterilizing effect, relieves skin, and has unique scent. Reynoutria It grows well in the wetted ground, looks like tiger skin, and has great anti-inflammatory and anti-oxidation effect. BIORAN Infla-Care relieves inflammatory skin problems such as atopy and acne as it greatly inhibits bacterium inducing dermatitis and can be widely applied to skin, body, and hair care products as it does not have toxicity. As well, it is valuable as anti-inflammatory ingredient as it rapidly relieves skin inflammation from external stimuli. Efficacy Effect 1. Anti-inflammation Great anti-inflammatory effect as it inhibits cytotoxicity by LPS and creation of NO No irritation 2. Anti-oxidation Other Details Recommend dosage: concentration 0.5~5 % Storage: seal, room temperature Application: Skin & Hair Care Product Available for formulation of anti-inflammation, anti-microbial, and relieving

BIORAN Infla-Care (Anti-inflammation) Magnolia (Magnolia kobus Flower) It is originated from lotus on trees and the language of flower is nobility Pain-killing, anti-inflammatory effect Effective on various inflammation related nose such as rhinitis and sinus infection Chrysanthemum (Chrysanthemum indicum Linne) Perennial herb belonging to chrysanthemum family Excellent for detoxification, anti-inflammation, purification of the blood Anti-inflammatory, anti-allergy, anti-oxidation, anti-viral, and whitening effect Rosemary (Rosemarinus officinalis) Well-known perennial herb with unique scent Strong anti-oxidation, disinfectant, and anti-microbial effect Reynoutria (Reynoutria elliptica) An important ingredient for honey of honeybee Strong anti-oxidation and anti-inflammatory effect

BIORAN Infla-Care (Anti-inflammation) 목련 Magnolia Magnolol & Honokiol Anti-inflammation and anti-microbial effect Chrysenthemum 감국 Rosemary Apiginin : Anti-inflammation, anti-allergy, anti-oxidation, capillary vessel strengthening effect Luteolin : Anti-oxidation and anti-virral effect Acacetin : Anti-oxidant and whitening (tyrosinase inhibitor) Rosemarinic acid Anti-inflammation, anti-oxidation, strengthening immune system, and skin protection from UV Labiatic acid, Canoside acid : Anti-oxidation Rosmaricine : Painkilling effect In addition, Carnosol, α-pinene, camphene, 1,8-cineole, borneol, camphore, α, β-terpineol, verbenol, dorneolacetate, etc Reynoutria Polygonin, Emodin, Emodin monoethyl ether, chrysophanic acid, chrysophanol, Hyperin, etc Anti-inflammation, anti-oxidantion, anti-microbial effect, etc

BIORAN Infla-Care (Anti-inflammation) Among various immune cells in human body involving inflammation, the researches were performed using Macrophage which has been performed a lot.

BIORAN Infla-Care (Anti-inflammation) There are many different materials inducing inflammation. Among them, this research was performed with LPS originated from microorganism.

BIORAN Infla-Care (Anti-inflammation) Inflammation inducing material Only NF-κB goes into a cell nucleus. (MAPK helps NF-κB be active) (In cell nucleus) NF-κB vitalizes COX-2 and inos Inflammation * COX-2 and inos cause inflammation as it encounters NF-κB and acts actively. Inflammation Mechanism: Restrains activities of COX-2, inos, NF-ĸB, MAPK, etc

NO conc (um) BIORAN Infla-Care (Anti-inflammation herbal complex) - Efficacy in vitro 18.0 16.0 14.0 12.0 10.0 8.0 6.0 4.0 2.0 0.0 Up to - 98% 0.5% 1% Anti-inflammatory effect has been proved through inhibition of NO induced by LPS We measured efficacy of inhibitory production of nitric oxide which stimulates inflammation induced by LPS(100ng/ml) in RAW264.7 macrophage. BIORAN Infla-Care effectively reduces production of NO concentration dependently. BIORAN Infla-Care is an excellent ingredient to relieve inflammation rapidly.

BIORAN Infla-Care (Anti-inflammation herbal complex) - - Efficacy in vitro Derma Vega BSASM Sel AN Calm stop LPS(+) LPS(-) 0.1% 0.5% 1% 5% BIORAN Infla-Care Anti-inflammatory effect has been proved through inhibition of NO induced by LPS We measured efficacy of inhibitory production of nitric oxide which stimulates inflammation induced by LPS(100ng/ml) in RAW264.7 macrophage. BIORAN Infla-Care effectively reduces production of NO concentration dependently. (The brighter the color is, the more production of NO is inhibited.)

Cell viability(%) BIORAN Infla-Care (Anti-inflammation herbal complex) - - Efficacy in vitro 140 120 100 80 60 40 20 BIORAN Infla-Care Derma calm Vega stop BSASM Sel AN 0 0.1% 0.5% 1% 5% Test of Cytotoxic Evalution (MTT assay) We carried out MTT assay after dealing with LPS(100ng/ml) and extracts in RAW264.7 macrophage. BIORAN Infla-Care has no cytotoxicity under 5% concentration.

DPPH radical scavenging activity (%) BIORAN Infla-Care (Anti-inflammation herbal complex) - - Efficacy in vitro 120 100 80 60 40 84% 95% 0.5% 1% 20 0 We carried out DPPH assay to assure anti-oxidation effect of BIORAN Infla-Care. BIORAN Infla-Care shows anti-oxidation effect concentration dependently. BIORAN Infla-Care shows over 80% of anti-oxidation effect at 0.5% concentration.

BIORAN Infla-Care (Anti-inflammation herbal complex) - Clinical Performance (Case 1) In 30 minutes after applying BIORAN Infla-Care Skin changes after use: relieve itching rapidly, relieve redness, and soothing effect

BIORAN Infla-Care (Anti-inflammation herbal complex) - Clinical Performance (Case 2) In 30 minutes after applying BIORAN Infla-Care Skin changes after use : relieve redness and soothing effect

BIORAN Infla-Care (Anti-inflammation herbal complex) - Clinical Performance (Case 3) In a week after applying BIORAN Infla-Care Skin changes after use : relieve redness and soothing effect

BIORAN Infla-Care (Anti-inflammation) SPECIFICATION Product name : BIORAN Infla-Care ITEMS SPECIFICATION Results 1. Appearance Light brown liquid Verified 2. Odor Characteristic Verified 3. ph 5.0 ± 1 5.25 4. Microorganism Below 100 cfu/ml Not detected 5. Heavy Metal Lead: below 20ppm, Arsenic: below2ppm Not detected Storage Conditions Keep airtight container in a room-temperature. Recommend Dosage 0.5 to 5%