Title:Eplerenone restores -h blood pressure circadian rhythm and reduces advanced glycation end-products in rhesus macaques with spontaneous hypertensive metabolic syndrome Yan Zhang,,Wen Zheng,, Yuli Liu,, Jue Wang,, Ying Peng, Haibao Shang,, Ning Hou,, Xiaomin Hu,, Yi Ding,, Yao Xiao,, Can Wang,, Fanxin Zeng,, Jiaming Mao,, Jun Zhang,, Dongwei Ma,, Xueting Sun,, Chuanyun Li,, Rui-Ping Xiao,, *, Xiuqin Zhang, * State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing 87, China. Peking-Tsinghua Center for Life Sciences, Beijing 87, China. Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing 87, China. Department of Surgery, Third Affiliated Hospital of Peking University, Beijing 87, China. *Corresponding author: Xiuqin Zhang, M.D., Ph.D. Institute of Molecular Medicine, Peking University, Beijing 87, China. E-Mail: zhangxq@pku.edu.cn Tel: (86)--67-. Fax: (86)--676-7. Rui-Ping Xiao, M.D., Ph.D. Institute of Molecular Medicine, Peking University, Beijing 87, China. E-Mail: xiaor@pku.edu.cn Tel: (86)--67-7. Fax: (86)--676-7.
Supplemental Material SUPPLEMENTAL FIGURES Supplemental Fig. Correlation between telemetric and cuff BP. Systolic blood pressure (a) and diastolic blood pressure (b) of monkeys with telemetric implants ( control normotensive and 7 metabolic syndrome hypertensive) measured simultaneously with a mercury sphygmomanometer (cuff) and telemetry min after anesthesia with ketamine ( mg/kg, i.m.). Supplemental Fig. Plasma concentrations of potassium (a), TCh (b), TG (c), ALT and AST (d), urea (e), and creatinine (f) of MetS hypertensive (n = 7) monkeys at baseline, treated with Eplerenone for days ( mg/kg/day, p.o., once a day) and washed out for days. Data are expressed as mean ± SEM. MetS, metabolic syndrome; TCh, total cholesterol, TG, Triglyceride; ALT, aspartate aminotransferase; AST, alanine aminotransferase. Supplemental Fig. Plasma concentrations of adiponectin (a), leptin (b), MCP- (c), and TNF-α (d) of the MetS hypertensive monkeys at baseline and after days of Eplerenone treatment. Data are expressed as mean ± SEM. MCP-, monocyte chemoattractant protein-; TNF-α, tumor necrosis factor α.
a Systolic Blood Pressure b Dias tolic Blood Pres sure Blood pressure by cuff (mmhg) y =.9x +.9 R =.967 Blood pressure by telemetry (mmhg) Blood pressure by cuff (mmhg) y =.88x +.78 R =.77 Blood pressure by telemetry (mmhg) Supplemental Figure
a b c Washout days Potassium (mmol/l)... TCh (mmol/l)... TG (mmol/l).... d e f Plasma concentration (U/L) ALT AST... Urea (mmol/l) 6... Creatine ( mol/l) 8... Supplemental Figure
a b Adiponectin (ng/ml) P =. Leptin (ng/ml) P =. c P =.89 d P =.6 MCP- (pg/ml) TNF- (pg/ml) Supplemental Figure
Supplemental Table. F value of the BP Circadian Rhythm in Control Normotension and MetS Hypertension Monkeys Group Animal ID# SBP DBP MBP HR.6 Control normotension.6.6 MetS hypertension.7 6.69 7 MetS indicates metabolic syndrome; SBP, systolic blood pressure; DBP, diastolic blood pressure; MBP, mean blood pressure; HR, heart rate.
Supplemental Table. F value of the BP Circadian Rhythm before and after Eplerenone treatment in MetS Hypertension Monkeys Group Animal ID# SBP DBP MBP HR.9.6.9. 6.69 7.8 Eplerenone.9 6.67 7 MetS indicates metabolic syndrome; SBP, systolic blood pressure; DBP, diastolic blood pressure; MBP, mean blood pressure; HR, heart rate.