905 Errata that were The following figures are four-color representations of figures incorrectly published in black and white in the July and August issues. Immunohistochemical Expression of Extracellular Matrix Components of Normal and Healing Periodontal Tissues in the Beagle Dog Masahiro Matsuura, Yeek Herr, Kyung-Yun Han, Wen-Lang Lin, Robert J. Genco, and Moon-Il Cho Journal of Periodontology 1995; 66:579-593 Figure 1. Morphology of a normal furcation area (hematoxylin and eosin [H&E] staining), a Low magnification view of a furcation area. = alveolar bone; C = cementum; BM = bone marrow; D = dentin. b High magnification of the periodontal ligament (PDL). c The fornix area offurcation. BV = Blood vessel, d High magnification of the junction between the PDL and cementum in sagittal section. Note the darkly stained matrix components around the fibers in the junction and Sharpey's fibers in cross section (arrowheads). Inset: A mesio-distal section demonstrating Sharpey'sfibers (arrowheads): CC = cementocyte.
906 ERRATA J Periodontol October 1995 Figure 2. Immunohistochemical staining for matrix components in normal periodontal tissues, a Immunostaining for FN. Note the homogeneous staining in the PDL and intense staining (arrowheads) at the attachment zone between the PDL and cementum. b Staining for type I collagen. Note the homogeneous staining in the PDL. c, d Staining for SPARC in a mesio-distal (c) as well as sagittal (d) section, c Note moderate staining in the PDL, but strong staining at the attachment zones between the PDL and cementum (arrowheads), and the PDL and bone, à Intense staining (arrowheads) is observed around Sharpey's fibers, e,f Staining for BSP in a mesio-distal (e) and a sagittal section, (f). e Note weak staining in the PDL, but strong staining at the attachment zone between the PDL and cementum. f The matrix around Sharpey's fibers, particularly in the superficial area, (arrowheads) is strongly stained, g, h Immunostaining for VN. Note strong staining in the PDL, and at the attachment zone (arrowheads) between the PDL and cementum in a mesio-distal section (g). In a sagittal section (h), VN staining is found around collagen fibers in the PDL and Sharpey 's fibers at the attachment zone. Localized intense staining is observed at the attachment zone (arrowheads).
Volume 66 Number 10 ERRATA 907 Figure 2. (Continued) i, j Controls for immunostaining for Cl (i) and BSP (j) are negative. The sections were counterstained with hematoxylin.
908 ERRATA J Periodontol October 1995 Figure 3. Morphology of a furcation defect and immunostaining for CI at 2 weeks after defect creation, a Note granulation tissue (GT) in the lesion and the newly-formed fibrous connective tissue (arrowheads) on the root surface (H&E staining), b Note intense staining for CI in the newly-formedfibrous connective tissue on the root surface (arrowheads), dense connective tissue (DCT) on bone (B), PDL, and dentin (D). The granulation tissue (GT) is unstained, c A high magnification view of the rectangular area in Fig. 3a. Note fibrous connective tissue on the root surface, (d, e) Note strong immunostaining for CI in fibrous connective tissue (arrowheads) on a coronal (d) and an apical portion of the root surface (e), but the absence of staining in granulation tissue containing numerous inflammatory cells (IC).
Volume 66 Number 10 ERRATA 909 Figure 4. Immunostaining for FN, CI, and VN in periodontal tissues at 2 weeks after defect creation, a Note the intense staining for FN around blood vessels (BV; arrow) and in the intercellular spaces of inflammatory cells (IC; arrowhead), b Note intense staining for FN in fibrous connective tissue on the root surface (arrowheads), c, d Morphology of a junction between granulation tissue andfibrous connective tissue showing the projected collagen fibers (arrowheads) and their associated fibroblasts (arrowheads, inset) into inflammatory cells (IC) (c), and collagen fibers (arrowheads) stained stronglyfor CI (d). e,fimmunostaining for VN. Note staining for VN in fibrous connective tissue (arrowheads), PDL, bone (B) and dense connective tissue (DCT) (e), f High magnification of the rectangular area in Fig. 4e. Note strong staining for VN in the connective tissue (*) adjacent to inflammatory cells (IC) and in the intercellular spaces between inflammatory cells. Blood vessels (BV) are not stained. s
910 ERRATA J Periodontol October 1995 Figure 5. Immunostaining for CI and BSP in fibrous connective tissue, a Morphology of dense connective tissue between the bone (B) and granulation tissue (H&E staining), b Strong immunostaining for CI was observed in dense connective tissue (DCT) and bone (B), but not in granulation tissue (GT). c, d In the area of inactive bone formation (B), immunostaining for BSP is faint in dense connective tissue and granulation tissue (GT) (c). However, in the active bone-forming area, the newly-formed bone (B) as well as the areas adjacent to active bone formation in the dense connective tissue reveal intense stainingfor BSP (arrowheads) (d).
Volume 66 Number 10 ERRATA 911 Figure 6. Morphology of a furcation defect and immunostaining for CI, SPARC, VN, and BSP in periodontal tissues at 4 weeks after defect creation, a Low magnification view of a furcation defect filled with dense connective tissue (DCT) and new bone (B). b High magnification view of the rectangular area in Figure 6a. Note numerous bone trabeculae (B) and dense connective tissue (DCT). c, d Immunostaining for CI. Low magnification view (c) shows intense staining for CI in dense connective tissue (DCT) and moderate staining in new bone (B). At high magnification (d), both dense connective tissue and osteoid (arrowheads) reveal strong staining for CI. >
912 ERRATA J Periodontol October 1995 Figure 6. (Continued) e,f, g, h immunostaining for SPARC, e Note SPARC staining in PDL and cementum, osteoid and bone (B).f, g Note staining in new cementum (C) (arrowheads), moderate staining in PDL and new bone, but weak staining in dense connective tissue (DCT). h Note intense staining in a résorption lacunae filled with new cementum (arrowheads), i, j Immunostaining for VN. Note strong staining in the PDL, endosteum (arrowheads), dense connective tissue (DCT) (i) and repairing cementum lacuna (arrowhead).
Volume 66 Number 10 ERRATA 913 Figure 6. (Continued) k,l,m,n Immunostaining for BSP. Strong staining is primarily observed in the newlyformed bone (B) and cementum (arrowheads, m, n), while the POL and dense connective tissue (DCT) are faintly stained (k). Interestingly, in the POL, the areas adjacent to active bone formation demonstrate patchy patterns of intense staining for BSP (I, m, arrows), whereas other areas inactive in bone formation are not stained. (o,p) Controls for SPARC (o) and for BSP ( ) do not show significant immunostaining (counterstained with hematoxylin). s
914 ERRATA J Periodontol October 1995 Figure 7. Morphology of a defect area and immunostaining for CI and FN 8 weeks after defect creation, a Low magnification view of a nearly regenerated furcation. Note the new PDL, bone and connective tissue at the fornix offurcation, b Immunostaining for CI. Note intense staining in connective tissue, Sharpey's fibers (arrowheads) and at the attachment zone of connective tissue to the root surface at the fornix of furcation (arrows), c Immunostaining for FN at the fornix offurcation. Note strong staining at the reattachment zone of Sharpey's fibers (arrowheads) to the root surface (arrows) and connective tissue. However, the areas where reattachment of connective tissue to the root surface is not yet established remain unstained (arrows), e Immunostaining for FN at the attachment zone between the PDL and cementum (arrowheads), but not at the zone between the PDL and bone (arrow), d, f Controls for FN staining at the fornix (d) as well as lateral (f) portions of a furcation area do not show any significant staining.