Cameron Smeal GELITA Australia, Beaudesert, Queensland, Australia Paul Truong Veticon Consulting, Brisbane, Queensland, Australia

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CURRENT VETIVER SYSTEM RESEARCH, DEVELOPMENT AND APPLICATIONS AT GELITA APA, AUSTRALIA Cameron Smeal GELITA Australia, Beaudesert, Queensland, Australia Paul Truong Veticon Consulting, Brisbane, Queensland, Australia

Australia Beaudesert

Previous findings Very high tolerance of vetiver to nitrogen/phosphorus More than 8 t/ha N, 800kg/ha P Extremely high nutrient uptake capacity Very high biomass generation, approximately 130 t/ha Younger shoots palatable to stock

100 Tolerance to extremely high levels of nutrients Shoot Dry Weight (g/pot) 80 60 40 20 100 0 0 200 400 600 800 1000 P Application (kg/ha/yr) Shoot Dry Weight (g/pot) 80 60 40 20 0 0 2000 4000 6000 8000 10000 N Application (kg/ha/yr)

NITROGEN UPTAKE 1200 1,140 1000 N kg/ha/year 800 600 400 600 500 360 250 200 90 0 Vetiver Dryland Rhodes Grass Kikuyu Grass Forage Sorghum Rye grass Eucalyptus Plant Species

PHOSPHORUS UPTAKE 160 149 P kg/ha/year 140 120 100 80 60 40 20 0 90 90 70 70 15 Vetiver Dryland Rhodes Grass Kikuyu Grass Forage Sorghum Rye grass Eucalyptus Plant Species

SOME VETIVER SYSTEM APPLICATIONS IN AUSTRALIA Industrial effluent disposal Domestic effluent disposal Landfill leachate disposal Wetland Floating pontoons

Industrial effluent disposal at GELITA

Domestic Effluent disposal in Brisbane. Sugarcane Banana Vetiver Toilet Septic Effluent

Six months after planting this stand of 100 plants absorbs all the discharge from the toilet block

Landfill leachate disposal in NSW, Australia

Wetland to treat municipal sewage effluent

Hydroponic treatment of municipal sewage effluent

CURRENT VETIVER RESEARCH, DEVELOPMENT AND APPLICATIONS AT GELITA Soil Based Reed Beds Agrochemical Retention and Disposal Evapo-transpiration of Vetiver Grass Vetiver Essential Oil for Pest Control and Pharmaceutical Uses Effects of Vetiver Grass on Some Soil Physical and Chemical Properties

SOIL BASED REED BED OBJECTIVES GELITA Australia initiated this field research to: Demonstrate the suitability of vetiver grass for use in the SBRB system to treat nitrogen rich industrial effluent Use the research findings in order to develop and establish a SBRB system that is capable of purifying GELITA s wastewater to a satisfactory level Develop a SBRB system using vetiver grass suitable for the treatment of high strength wastes both in Australia and world wide.

Soil Based Reed Beds The SBRB system has three simple components: A shallow bed of soil, sand or gravel A suitable wetland plant Micro-organisms (fungi and bacteria)

Reed bed construction at GELITA, Australia 4m 40m

New planting on sand bed Sampling points

Soil Based Reed Beds with clay, sand and gravel beds at GELITA

AGROCHEMICAL RETENTION AND DISPOSAL OBJECTIVES To demonstrate the benefits of vetiver grass filter strips on farms in reducing herbicide movement into the aquatic environment To develop a simple system suitable for easy adoption by the farming community to improve water quality To promote the adoption of the Vegetative Buffer Strip for Soil Erosion & Deposition model developed by Griffith University, Brisbane, Australia

Atrazine Capture and Retention Project Pond Vetiver

18 month old vetiver ready for treatment Atrazine Capture and Retention Project

Atrazine Capture and Retention Project ready for trial

Nine sets of sampling points, each at 3 depths: 20cm, 50cm and 100cm

Boring for installation of monitors

FullStop subsoil Hand pump seepage monitor Depth Collecting tube Seepage Collector

A set of sampling point with 3 collectors at 20cm, 50cm and 100cm depth 20cm 100cm 50cm

EVAPO-TRANSPIRATION OF VETIVER GRASS OBJECTIVES The principal objective is to determine accurately the transpiration rates The evapo-transpiration rate of vetiver reported in the literature varied from 3.8 mm/day in summer and 1.9 mm/day in winter under field conditions to 43 mm/day under effluent treatment conditions. These are up to 4-10 times greater than normal rates. If such extreme claims are incorrect, environmental damage could result from undersized effluent irrigation schemes.

Evapotranspiration

Plot Design for Transpiration Study (A. Vieritz)

Experimental Design Experimental Design 12 bays of 3m _ 3m in area, 1.45 m deep and 4m apart, orientated perpendicular to the predominant wind direction. 4 treatments _ 3 replicates Treatments 1- Vetiver (Chrysopogon zizanioides) 2- Kikuyu (Pennisetum clandestinum) a pasture grass 3- Broad leaf Cumbungi (Typha orientalis) a wetlands species endemic to the area 4- Control (bare ground)

3m x 3m x 1.45m deep bay lined with heavy waterproof membrane

Sampling outlet Inlet

Twelve bays all set ready for planting

VETIVER ESSENTIAL OIL FOR PEST CONTROL AND PHARMACEUTICAL USES OBJECTIVES Literature search has indicated that vetiver essential oils (VO) have Antibacterial, Antifungal, Antioxidant and Antiinflammatory attributes. The aims of this project are to: Determine the best extraction methods for _-vetivone and _- vetivone and possibly other components as well. Determine which Vetiver species has highest content of α- vetivone and β-vetivone at which stage of growth. Determine whether VO can be extracted from leaves Whether heavy metals and other chemicals will affect the quality and quantity of VO Whether VO contains heavy metals and other chemicals when used for phytoremediation

Hydro distillation Extraction Methods Steam distillation

Solvent extraction

EFFECTS OF VETIVER GRASS ON SOME SOIL PHYSICAL PROPERTIES OBJECTIVES Due to its extensive, deep and penetrating root system, it is expected that some soil physical and chemical parameters would be improved under vetiver cultivation The aims of this project are to: Monitor the changes in hydraulic conductivity and soil bulk density Monitor the changes in salinity and soil chemistry on various soils within the GELITA property Provide soil-water parameters, which will determine appropriate irrigation management and monitoring programs

Determining saturated hydraulic conductivity Testing Guelph Permeameter Guelph Permeameter in the field

Variation in root growth by soil type 700mm

Thank You