IR-4 Ornamental Horticulture Program Research Report Cover Sheet

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20150327i.pdf IR4 Research Report over Sheet Researcher(s) Tom Freiberger Affiliation: USDAARS Rutgers University IR4 Ornamental Pro 283 Rt. 539 ream Ridge NJ 08514 PhoneNumber: (609) 7587311 Email: freiberger@aesop.rutgers.edu Trial: 2014 NJ ProjectTitle: yflumetofen rop Safety Protocol #: 14008 PR# Research Target rop/plant Product EPA Reg. # Production Site Status 31731 Angelonia Angelonia angustifolia Sultan yflumetofen BASF 31724 alibrachoa alibrachoa sp. Sultan yflumetofen BASF 31738 Dracaena Dracaena sp Sultan yflumetofen BASF ProjectTitle: Pyrfluquinazon rop Safety Protocol #: 14008 PR# Research Target rop/plant Product EPA Reg. # Production Site Status 31689 alibrachoa alibrachoa sp. Rycar (SP3009/NNI0101) 71711376769 31691 Tickseed oreopsis sp. Rycar (SP3009/NNI0101) 71711376769 31696 Dracaena Dracaena sp Rycar (SP3009/NNI0101) 71711376769 29325 Pansy Viola sp. Rycar (SP3009/NNI0101) 71711376769 ProjectTitle: Tolfenpyrad rop Safety Protocol #: 14008 PR# Research Target rop/plant Product EPA Reg. # Production Site Status 31697 alibrachoa alibrachoa sp. HachiHachi S Tolfenpyrad 31699 Tickseed oreopsis sp. HachiHachi S Tolfenpyrad 31704 Dracaena Dracaena sp HachiHachi S Tolfenpyrad

p 1 Project Title: rop Safety of New Insecticides and Miticides Protocol #: 14008 PRnumbers:29325, 31731, 31697, 31689, 31724, Narrative Summary (Results/Discussion) Please keep text to one page if possible. Include summary of trial results and a brief discussion including how any changes from the protocol may have affected results. Results for multiple PRnumbers can be summarized together, but please list all PRNumbers in the header and in the summary data table. Pansy, Dracaena, alibrachoa and oreopsis were purchased as plugs from Kube Pak and potted up on 11/25/2014 in fafard #2 growing mix (70% peat, 20% perlite and 10% vermiculite) using classic 300S pots. Angelonia were purchased as seeds from Stokes seed and seeded on 11/3/2014 in classic 300S pots and fafard #2 growing mix. Dracaena and pansy were placed in greenhouse #1 while alibrachoa and oreopsis were placed in greenhouse #2. All pots were placed on a black woven porous weed mat and both greenhouses had overhead irrigation on a timer and overhead and floor heat. Osmocote Plus 15912 granular fertilizer was applied at a rate of 6 grams per pot. Beginning plant height and width measurements were done on 12/19/2014 for Dracaena and oreopsis, 12/22/2014 for alibrachoa and Pansy and 1/5/2015 for Angelonia. The first application was done on 12/23/2014, the second application was done on 1/6/2015 and the final application was done on 1/20/2015. For the Angelonia, the first application was done on 1/6/2015. The second application was done on 1/20/2015 and the final application was done on 2/3/2015. All foliage was dry prior to all applications and a single nozzle boom was used to treat all 12 pots of the same treatment sprayed over the top on a single pass. Plants of the same treatment were sprayed out of the same spray bottle mixture. This procedure was used for all foliar applications. ratings were taken every week after the initial application for six weeks. Final plant height and width measurements were taken on 2/3/2015 and 2/18/2015 for Angelonia. See protocol 14008 GH#1, 2 spreadsheet for evaluation ratings. Results Table Please insert results table here. Include PRnumbers for each treatment if multiple PRnumbers are included in this summary. Please include product, active ingredient, and statistics. See protocol 14008 GH#1, 2 spreadsheet. No stats were run on this data. The Dracaena spikes and oreopsis were not affected in any way at any of the rates by their applications. alibrachoa suffered no damage at any rate with Hachihachi S and SP3009. Both of these chemicals looked like they helped kill off the aphids and thrips that were damaging the other groups of alibrachoa since these groups suffered little to no insect damage. alibrachoa applied with Sultan and both control groups suffered major damage from thrips and aphids. Maintenance sprays were done to try to kill them off, but we had trouble controlling them. Once we did get control of the aphids and thrips, all plants began to improve and grow vigorously again. This was not until after the pictures were taken and the final evaluation was done. New, updated pictures were taken (3/4/2015) to show the improvement. The pansies had a few die off, but otherwise the rest grew well having SP3009 not affecting them. Angelonia suffered no damage from Sultan with the few that died being from natural causes. Being planted from seed, not enough grew so we had to exclude the surfactant only control group.

p 2 Project Title: rop Safety of New Insecticides and Miticides Protocol #: 14008 PRnumbers:29325, 31731, 31697, 31689, 31724, Materials & Methods/Recordkeeping Please fill out the information below or attach a separate document with comparable information. Name(s) of Personnel onducting Research: Dave Bodine, Tom Freiberger Location of Trial (city/state): ream Ridge, NJ Use Site (greenhouse/shadehouse/field container/etc): rop History Pansy: Matrix Blue Frost, Angelonia: Serena Blue, Dracaena: Spikes, alibrachoa: rop ultivar/variety: Kabloom White, oreopsis: Early Sunrise Yellow Date of Seeding: Angelonia 11/3/2014 Date of Emergence: Angelonia 11/12/2014 Date of Transplanting: Pansy, Dracaena, alibrachoa, oreopsis 11/25/2014 Potting Mix: Fafard #2 Growing Mix (70% peat, 20% perlite, 10% vermiculite) Pot size & spacing: lassic 300S,.66 Row spacing: NA Product(s) applied prior to start of experiment: Product Rate Type Date of rop Growth Stage Volume Osmocote Plus 159 12 6 grams per pot Granular 11/26/2014 Vegetative NA Awaken (Foliar fertilizer) 10 ml. per 1 gal. Foliar 11/26/2014 Vegetative TopsinM 70WSB (thiophanatemethyl) 12 oz per 100 Foliar 11/26/2014 Vegetative Warrior II with Zenon Technology (Lambdacyhalothrin) Admire II (Imidacloprid) 5 fl oz per 100 (GH #2, 3 fl oz per 100 (GH #2, Foliar 12/12/2014, 12/15/2014 Vegetative Foliar 12/19/2014 Vegetative Add more rows as needed.

p 3 Project Title: rop Safety of New Insecticides and Miticides Protocol #: 14008 PRnumbers:29325, 31731, 31697, 31689, 31724, Experiment Information Experimental Design: Number of Reps: 12 pots per treatment Materials & Methods: Insert materials & methods here only if information is not presented elsewhere. Include any changes from protocol. Equipment: O 2 backpack sprayer with single nozzle boom Product(s) applied during experiment (including treatments, fertilizers, etc): Product Rate(s) Type Date of rop Growth Stage Hachihachi S 21 fl oz per 100 (tolfenpyrad) SP3009 (pyrifluquinazon) 42 fl oz per 100 84 fl oz per 100 6.38 fl oz per 100 12.76 fl oz per 100 25.52 fl oz per 100 Sultan (cyflumetofen) 13.7 fl oz per 100 27.4 fl oz per 100 54.8 fl oz per 100 Onyx Pro (bifenthrin) 8 fl oz per 100 (GH #2, Hachihachi S (tolfenpyrad) Overture (pyridalyl) Add more rows as needed. 30 fl oz per 100 (GH#2, 8 oz per 100 (GH #2, Foliar 12/23/2014, 1/6/2015, 1/20/2015 Foliar 12/23/2014, 1/6/2015, 1/20/2015 Foliar 12/23/2014, 1/6/2015, 1/20/2015, (2/3/2015: Angelonia only) Foliar 1/13/2015 Foliar 1/26/2015 Foliar 1/26/2015, 1/29/2015 Volume

p 4 Project Title: rop Safety of New Insecticides and Miticides Protocol #: 14008 PRnumbers:29325, 31731, 31697, 31689, 31724, Photos Please embed photos here or send jpg, tiff, or bmp. Sent separately. Data ollected Please describe data collected and scoring system. Also include the dates data were collected. Plants were evaluated on a scale of 010. 0 being no phytotoxicity and 10 being a complete kill. Beginning plant height and width measurements taken on 12/19/2014 for Dracaena and oreopsis, 12/22/2014 for alibrachoa and Pansy, 1/5/2015 for Angelonia. The first application was done on 12/23/2014, for angelonia 1/6/2015. The second application was done on 1/6/2015, for angelonia 1/20/2015. The third application was done on 1/20/2015, for angelonia 2/3/2015. The first evaluation was done on 12/30/2014 and 1/13/2015 for angelonia. The second evaluation was done on 1/6/2015 and 1/20/2015 for angelonia. The third evaluation was done on 1/13/2015 and 1/26/2015 for angelonia. The fourth evaluation was done on 1/20/2015 and 2/3/2015 for angelonia. The fifth evaluation was done on 1/26/2015 and 2/10/2015 for angelonia. The sixth evaluation was done on 2/3/2015 and 2/18/2015 for angelonia. Final height and width measurements were done on 2/3/2015 and 2/18/2015 for angelonia. Pictures were taken on 2/4/2015 and 2/18/2015 for angelonia. Picture retakes for alibrachoa were done on 3/4/2015. Raw Data Insert raw data below or send separate file containing raw data. See protocol 14008 GH #1, 2 spreadsheet.

p 5 Project Title: rop Safety of New Insecticides and Miticides Protocol #: 14008 PRnumbers:29325, 31731, 31697, 31689, 31724, Environmental conditions during the experiment: Insert temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Or send separate file with this information. Include a statement about any significant weather or environmental events during the course of the experiment. #1 and GH#2 had four overhead sodium lamps that provide additional lighting between 6 a.m. and 10 p.m. Overhead heat was set to 66 o F and floor heat was set to 62 o F. The exhaust fans and louvers were set to 88 o F. Overhead Irrigation for #1 Date Amount in inches 11/2511/28.125 11/29/20142/18/2015.25 Overhead Irrigation for #2 Date Amount in inches 11/2511/28.125 11/2912/22.25 12/23/20141/20/2015.125 1/21/20152/4/2015.25 Irrigation timers were set to either 15, 30 or 60 minutes depending on the amount needed. 15 minutes equals.125, 30 minutes equals.25 inches and 60 minutes equals.5 inches.