Test Code. Solid food & feed HEC51. Solid food & feed HEC52. Liquid food HEC50. Sugars in coffee Coffee HEC1M HPAEC-PAD ISO Fructose

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Standard portfolio Eurofins Carbohydrate Competence Centre Mono, di- and oligosaccharides Total sugars after hydrolysis Total reducing sugars (as glucose) Total reducing sugars (as lactose) & feed & feed HEC54 HEC62 HEC65 HEC66 HEC53 HEC67 Luff Schoorl NEN 3571 Luff Schoorl Luff Schoorl Carbohydrates HEC51 HEC59 Calculation Total digestible carbohydrates & feed HEC52 HEC60 Luff Schoorl Sugars in coffee Coffee HEC1M ISO 11292 Fructose HEC2M All other matrices HEC2I Glucose HEC2L All other matrices HEC2H Galactose (only with PHECB) Food & Feed HEC2F Xylose HEC83 HEC84 Food (exc. dairy)* HEC2N Lactose Dairy* HEC36 All other matrices HEC2J HEC61 Luff Schoorl Lactose free HEC79 Sucrose HEC2P All other matrices HEC2G Maltose HEC2Q All other matrices HEC2K Maltotriose Food HEC0C Trehalose HEC49 HEC50 Palatinose (Isomaltulose) HEC0U Kestose (GF2 from inulin) HEC0S Nystose (GF3 from inulin) HEC0T Raffinose/Stachyose/Verbascose HEC1K Malto-oligosaccharides (DP1-DP7) HEC0I Fructose oligomers (F2-F6) quant. For other matrices HEC0R

Starch and Dietary Fibre (incl. prebiotics) Starch total * HEC57 ISO 15914 Feed* HEC81 Enzymatic Meat & fatty HEC1R products Starch (incl. maltodextrins) HEC0L Enzymatic Damaged Starch Food HEC42 Enzymatic AACC 76-31 Gelatinized Starch Feed HEC2T Enzymatic AACC 76-31 Resistant Starch & Feed HEC87 Enzymatic AOAC 2002.02 Amylose & amylopectin in starch Food & Feed with >10% starch HEC0J Titration Amylose & amylopectin in sample Food & Feed with (only with PHE02) >10% starch HEC2X Calculation Starch/Maltodextrin and Carbohydrate composition of sol. & insol. NSP excl. cellulose Feed HEC2R / Colorimetric ( for more info) Classical dietary fibres, total HEC56 HEC63 Gravimetric AOAC 985.29 Breakfast cereals* HEC55 Classical total dietary fibres (TDF / IDF / SDF) HEC1Q Gravimetric AOAC 991.43 Total dietary fibres (HMWDF HEC1A (incl. RS) / LMWDF) AOAC 2009.01 Total dietary fibres (incl. RS) / LMWDF) (HMWDF Total dietary fibres (IHMWDF (incl. RS) / SHMWDF / LMWDF) Food with high starch content HEC1F HEC1B AOAC 2009.01 (revised) AOAC 2011.25 -glucan (yeast & moulds) with >40% -glucan HEC1J Enzymatic -glucan (cereals) high Glc content HEC1C Enzymatic AOAC 995.16 -glucan (cereals) Low Glc content HEC2Z Enzymatic AOAC 995.16 Pectin (as total uronic acid) HEC2D Colorimetric Total sialic acid Infant formula HEC2W HEC26 (>10% lactose), GOS (GOS) AOAC 2001.02 ingredients & HEC1H premixes (<10% lactose)

Starch and Dietary Fibre (incl. prebiotics) cont. (GOS) Degree of polymerization of GOS (DP2-DP8) Dietary fibre containing supplemented res. maltodextrin Inulin / Fructo-oligosaccharides (FOS) Polydextrose Dextrose equivalent (only in combination with red. sugars and moisture tests) All liquid samples (incl. syrups), GOS ingredients and premixes HEC1I / Solid & HEC1G HEC3D HEC3E HEC39 HEC38 HEC71 HEC72 GPC- RI Calculation AOAC 2001.02 AOAC 2001.03 AOAC 997.08 AOAC 999.03 AOAC 2000.11 Fingerprint (qualitative tests) Starch hydrolysate (maltodextrin) Inulin & Fructo-oligosaccharides For other matrices Solid ingredients Liquid ingredients For other matrices HEC0N HEC30 HEC31 HEC0P Polydextrose HEC0Q Sweeteners Isomalt HEC85 & HEG53 Sucralose solid food HEC74

Carbohydrate derivatives Total uronic acid HEC2D Colorimetric Galacturonic acid Glucuronic acid Packages Degree of gelatinization (calc. damaged starch / starch) Sugar profile (Glc, Fru, Lac, Suc, Mal + Gal) Total sugar incl. Gal (to order only together with PHECB) Sugar profile (Glc, Fru, Lac, Suc, Mal) Total sugar (to order only in combination with PHECC) Sugar profile (Glc, Fru, Lac, Suc, Mal) Total sugar (to order only in combination with PHECD) Pet food PHECA Calculation All matrices PHECB All matrices HEC3A Calculation PHECC Food HEC3B Calculation All matrices (except food) All matrices (except food) PHECD HEC3C Calculation Amylose / starch / amylopectin Starch & cereals PHE02 Nutritional Value Packages for products supplemented with low molecular weight dietary fibre (prebiotics, polydextrose, res. maltodextin etc.) PHEP9 Solid sugary products PHEPA PHEPB Liquid sugary products PHEPC Dietary supplements PHEP5

Decision tree for dietary fibre determination Contact: Eurofins Carbohydrate Competence Centre Leeuwarderstraatweg 129 8441 PK Heerenveen The Netherlands Tel. +31 88 8310000 Fax +31 88 8310010 Mail: carbohydrates@eurofins.com Web: www.eurofins.nl Web: www.carbohydratestesting.com version 01/2017