Medical School Histology Basics VIBS 289 lab Blood Larry Johnson Texas A&M University
Blood (definition and function) Blood - fluid tissue composed of erythrocytes (RBC), leukocytes (WBC), and platelets suspended in blood plasma. Function of blood is the transportation of cells and fluid. RBC carry O 2 to and CO 2 from tissues.
Function of blood is transportation of cells and fluid. WBC Immune defense for body. Plasma Nutrients to tissues. Waste from tissues. Proteins to hold water in plasma. Hormones and other informational mediators. Platelets Prevent loss of transportation.
Peripheral blood HISTO 021 Erythrocytes Reticulocytes
Reticulocyte Note ribosomes in the reticulocyte
Polychromatophilic normoblasts
Bone marrow 32583 Reticulocyte entering blood vessel Blood vessel
White Blood Cells Neutrophil Eosinophil Neutrophil Eosinophil Basophil Lymphocyte Monocyte Basophil Lymphocyte Monocyte Neutrophil Eosinophil Basophil Lymphocyte Monocyte
White blood cells in blood Eosinophil Neutrophil Basophil Lymphocyte Monocyte
Neutrophils platelets Neutrophils Large lymphocyte Monocyte Lymphocyte Neutrophils Red blood cells Slide 113
SIZE OF HUMAN BLOOD CELLS CELL/PLATELET SIZE ERYTHROCYTES 6.5-8 µm LEUKOCYTES (WBC) % of WBC NEUTROPHIL 12-15 µm 60-70% LYMPHOCYTE 6-18 µm 25% MONOCYTE 12-20 µm 5% EOSINOPHIL 12-15 µm 2-4% BASOPHIL 12-15 µm 0-1% PLATELETS 2-4 µm
110 110 Peripheral blood smear (Leishman-Giemsa) Monocyte Neutrophils Monocyte Neutrophils Lymphocyte Neutrophils Monocyte
110 110 Peripheral blood smear (Leishman-Giemsa) basophil, eosinophil, and neutrophils Lymphocyte Neutrophils Basophil Neutrophils Neutrophils
110 Peripheral blood smear (Leishman-Giemsa) eosinophil and neutrophil platelets Neutrophil Eosinophil Red blood cells Eosinophil Neutrophil
113 Slide 113 Peripheral blood smear (May-Grunwald-Giemsa) Monocyte Neutrophil Lymphocytes Monocyte Lymphocyte
113 Peripheral blood smear (May-Grunwald-Giemsa) Monocyte Lymphocyte Monocyte Monocyte
Slide 113 Peripheral blood smear (May-Grunwald-Giemsa) Lymphocyte
HISTO 021 HISTO 021 60x
HISTO 021
EM 8a EM 8c EM 8c illustrates an example of a basophil Note the neutrophils and eosinophils in EM 8a
140 Cardiac stomach w/chronic infection Infection site Plasma cells
140 140 Cardiac stomach w/chronic infection Eosinophil Neutrophil
EM 12b to observe organelle content differences in an immature and mature neutrophils mature has more mature dumbbellshaped granules EM 8f
EM 8f Lymphocytes
EM 6 Note the relative paucity of cytoplasmic organelles and the high nuclear to cytoplasmic ratio (EM 6) Lymphocytes
Large Golgi and many plasma membrane projects on monocytes. HISTO 021 Monocytes
Large Golgi and many plasma membrane projects on monocytes. Macrophages 432 (Slide 432) lung EM 8f Monocyte HISTO 021 1 Blood monocytes differentiate into macrophages like those seen in the lungs
Hematopoiesis Simplified: Part 1 Erythropoiesis https://www.youtube.com/watch?v=uto4lnxontk&list=plqltfhuki1sgtel3o9ipdsbgewmy3jax5&index=19 Larry Johnson Texas A&M University
Erythropoiesis Erythrocytes Reticulocytes No nucleus and red cytoplasm No nucleus and blue - grey cytoplasm Orthochromatophilic erythroblasts Polychromatophilic erythroblasts Dark, small, spherical nucleus blue grey cytoplasm Darkening, fractured,spherical nucleus and mixed pools of grey and blue cytoplasm Basophilic erythroblasts Proerythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Uniformly light, spherical nucleus with thin rim of medium to dark blue cytoplasm
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Erythropoiesis Erythrocytes Proerythroblasts Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis
Bone marrow 32583 Reticulocyte entering blood vessel Blood vessel
Bone marrow
EM 12a Bone marrow
Slide 112
Bone marrow
EM 8a marrow blood
EM 12b
EM 8b
EM 8f
EM 8b
432 432 Lung with bronchi macrophage
432 432 Lung with bronchi
432 Lung with bronchi
32583 EM 12c
Many illustrations in these VIBS Histology YouTube videos were modified from the following books and sources: Many thanks to original sources! Bruce Alberts, et al. 1983. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY. Bruce Alberts, et al. 1994. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY. William J. Banks, 1981. Applied Veterinary Histology. Williams and Wilkins, Los Angeles, CA. Hans Elias, et al. 1978. Histology and Human Microanatomy. John Wiley and Sons, New York, NY. Don W. Fawcett. 1986. Bloom and Fawcett. A textbook of histology. W. B. Saunders Company, Philadelphia, PA. Don W. Fawcett. 1994. Bloom and Fawcett. A textbook of histology. Chapman and Hall, New York, NY. Arthur W. Ham and David H. Cormack. 1979. Histology. J. S. Lippincott Company, Philadelphia, PA. Luis C. Junqueira, et al. 1983. Basic Histology. Lange Medical Publications, Los Altos, CA. L. Carlos Junqueira, et al. 1995. Basic Histology. Appleton and Lange, Norwalk, CT. L.L. Langley, et al. 1974. Dynamic Anatomy and Physiology. McGraw-Hill Book Company, New York, NY. W.W. Tuttle and Byron A. Schottelius. 1969. Textbook of Physiology. The C. V. Mosby Company, St. Louis, MO. Leon Weiss. 1977. Histology Cell and Tissue Biology. Elsevier Biomedical, New York, NY. Leon Weiss and Roy O. Greep. 1977. Histology. McGraw-Hill Book Company, New York, NY. Nature (http://www.nature.com), Vol. 414:88,2001. Arthur C. Guyton,1971.Textbook of Medical Physiology W.B. Saunders company, Philadelphia, PA WW Tuttle and BA Schottelius 1969 Textbook of Physiology C.V. Mosby Co. A.L. Mescher 2013 Junqueira s Basis Histology text and atlas, 13 th ed. McGraw
The end of
Hematopoiesis Simplified: Part 1 Erythropoiesis Larry Johnson Texas A&M Univers
Hematopoiesis Simplified: Part 1 Erythropo Objectives are to: Identify the developmental cells of erythropoiesis Characterize developmental changes in the cytoplasm and nucleus of these cells Bone marrow 32583 Blood vessel
19761 Bone marrow Blood in vessel
HISTO 021 60x
HISTO 021 60x
Erythropoiesis Erythrocytes Reticulocytes No nucleus and red cytoplasm No nucleus and blue - grey cytoplasm Orthochromatophilic erythroblasts Polychromatophilic erythroblasts Dark, small, spherical nucleus blue grey cytoplasm Darkening, fractured,spherical nucleus and mixed pools of grey and blue cytoplasm Basophilic erythroblasts Proerythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Uniformly light, spherical nucleus with thin rim of medium to dark blue cytoplasm
McGraw-Hill Companies
Erythropoiesis Erythrocytes Proerythroblasts Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis
Orthochromatophilic erythroblasts Erythrocytes
Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis
Polychromatophilic erythroblasts
Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis
Basophilic erythroblast
Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis
Proerythroblasts
Erythropoiesis Erythrocytes Orthochromatophilic erythroblasts Reticulocytes
Early orthochromatophilic erythroblasts Late polychromatophilic erythroblasts
Polychromatophilic erythroblasts Early polychromatophilic erythroblasts
Basophilic erythroblasts Proerythroblasts
RBCs Basophilic erythroblast Orthochromatophili c erythroblasts Late polychromatophilic erythroblast ut 112 platelets Reticulocyte Polychromatophilic erythroblast Eosinophil or basophil Erythrocytes Proerythroblast
Bone marrow Reticulocyte entering blood vessel 32583 Blood vessel
The End of Hematopoiesis Simplified: Part 1 Erythropoiesis
Granulopoiesis is next
Hematopoiesis Simplified: Part 2 Granulopoiesis Larry Johnson Texas A&M Univers
Hematopoiesis Simplified: Objectives are to: Part 2 Granulopoiesis Identify the developmental cells of granulopoiesis Characterize developmental changes in the 32583 cytoplasm and nucleus of these cells Bone marrow
19761 Bone marrow Blood Blood
32583 ` Bone marrow Developing granulocytes
21 Peripheral blood
Granulopoiesis Neutrophils Lobulated, dark nucleus, mature grey cytoplasm with small granules Neutrophilic band or stab cells Horse-shoe shaped, darkening nucleus, grey mature cytoplasm Neutrophilic metamyelocytes Neutrophilic myelocytes Promyelocytes Oval to kidney bean-shaped, indented nucleus, grey mature cytoplasm with a weak blue tent Large, oval, non-indented nucleus, large amount of cytoplasm with specific and non-specific granules Large, oval, non-indented nucleus, large amount of sky blue cytoplasm with non-specific granules
Granulopoiesis Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilic metamyelocytes
ut 112 Neutrophil Neutrophilic metamyelocytes Neutrophilic metamyelocyte Platelets Eosinophil or basophil Neutrophilic myelocytes
Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilic metamyelocytes Granulopoiesis
Neutrophilic metamyelocytes 112 Lymphocytes Unknown nuclei Neutrophilic myelocytes Promyelocyte Neutrophilic myelocytes Lymphocytes Neutrophilic band cells Neutrophilic band cells Neutrophilic metamyelocytes Neutrophilic band cell
Neutrophils Neutrophilic band cells Granulopoiesis
Neutrophilic metamyelocytes Early neutrophilic metamyelocytes
Neutrophilic myelocytes
Late promyelocytes Promyelocyte
Neutrophilic metamyelocytes Unknown nuclei Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Orthochromatophilic erythroblasts Proerythroblast Eosinophils or basophils Orthochromatophilic erythroblasts
32583 Bone marrow White blood cell entering a blood vessel Blood vessel Blood vessel
he End of Hematopoiesis Simplified: Part 2 Granulopo