Zongliang (Carl) Jiang 333 Cedar St, New Haven, CT 06510 860-786-8766 zongliang.jiang@yale.edu OBJECTIVE To conduct research and teaching in the areas of animal reproduction, epigenetics, and stem cell biology. EDUCATION Postdoctoral associate in Obstetrics, Gynecology & Reproductive Sciences, School of Medicine, Aug 2016 Present Yale University, New Haven, U.S.A Postdoctoral fellow in the Animal Science/Center for Regenerative Biology, Sep 2015 Aug 2016 University of Connecticut (UCONN), Storrs, CT, U.S.A Ph.D. Animal Science/Reproductive Physiology, Aug 2015 UCONN, Storrs, CT, U.S.A M.S, Animal Science, May 2012 UCONN, Storrs, CT, U.S.A Visiting Scholar in the Center for Regenerative Biology, Sep 2010-Jul 2011 UCONN, Storrs, CT, U.S.A BS (with Honors) in Animal Science, Jun 2008 Guangxi University, Nanning, Guangxi, China PROFESSIONAL EXPERIENCES Research Experiences: Functional genomics of single bovine in vivo pre-implantation embryos. RNA-seq data analyzed using the Bioconductor statistical suite. Methylome characterization of single bovine in vivo pre-implantation embryos by RRBS and WGBS. Characterization of global loss of imprinting in embryo and fetal development induced by assisted reproduction in domestic animals. 1
Effect of sub-lethal Hydrostatic Pressure (HHP) on transcriptional profiles of bovine in vitro-produced (IVP), vitrified blastocysts. Genes that changed expression due to HHP treatments were identified using Microarray and real-time quantitative RT-PCR, Data were analyzed using GeneSpring 12.1, Bioconductor statistical suite and SAS program. Roles of Brm and Baf170, components of chromatin remodeling complex, during reprogramming using gene-targeting approaches. Reprogrammed somatic cells by the ipsc technology using shrna-mediated knockdown in mouse ips and ES cell lines, ipscs characterization and cell culture. Investigated PRNP (gene caused mad cow disease) and Y chromosome gene targeting in bovine somatic cells using transcription activator-like effector and nuclease (TALEN) technique and CRISPR-CAS9 system. Transcriptional profiling of imprinted, X-linked, histone, mitochondrial and housekeeping genes in mammalian pre-implantation development. Data mining from public databases. Co-discovered the role of Jak/Stat3 signaling in epigenetic regulation of somatic cell reprogramming to Naïve pluripotency. Co-discovered the role of Akt in Stat3 activation and the isoform-specific roles of Akt in induced pluripotent stem cells (ipscs) generation. Generating and characterizing pluripotent bovine ipscs. Sequenced the complete mitochondrial genome of Oreochromisco aureus and analyzed their molecular phylogenesis. Studied the function of mouse gonad-specific expression gene. Co-investigated the genetic diversity between sexes of genetic improvement of farmed tilapia by Simple Sequence Repeat analysis. Teaching and Supervising Experiences: Teaching assistant for ANSC 3323/5621 (Animal Embryology and Embryo Biotechnology) in Department of Animal Science, UCONN, every fall since 2012. Training fellow graduate and undergraduate students on molecular cloning, ipsc generation and characterization and module analyses of RNA-seq data. Lab management: supply orders, material transfers, lab cell line storage/maintenance, research protocol development, lab instrument log, user training, and annual maintenance. Research Collaborations: Drs. Rachel O Neill, Jinbo Bi (UCONN); Dr. Yijun Ruan, Dr. Oscar Luo (Jackson Laboratory); Dr. Jinbo Chen (Xinjiang Academy of Animal Science, China) Project: Functional genomics of the bovine in vivo pre-implantation development. Dr. Csaba Pribenszky (Szent Istva n University, Hungary), Drs. Sadie L. Smith (CCSU), Drs. Lynn Kuo (UCONN) Project: Effect of sub-lethal Hydrostatic Pressure (HHP) on transcriptional profiles of bovine in vitro-produced (IVP) vitrified blastocysts. Dr. Young Tang (UCONN) Project: Roles of Brm and Baf170, components of chromatin remodeling complex, during reprogramming 2
Manuscripts Reviewed: 2016 Journal: Stem cells and Development, Scientific Reports, BMC developmental biology, PLOS ONE 2015 Journal: PLOS ONE 2015 Abstract Reviewing IETS 2016 - Stem cells section 2014 Journal: PLOS ONE 2014 Journal: PNAS Professional Membership: 2014-2016 American Association for the Advancement of Science (AAAS) 2014-2016 International Embryo Transfer Society (IETS) 2013 International Society for Stem Cell Research (ISSCR) Honors and Awards: 2014 Doctoral Dissertation Fellowship Award, UCONN, Storrs, CT 2010 State Scholarship Fund to pursue study in USA as a joint PhD student, China Scholarship Council, China 2009 Outstanding Graduate Student, Guangxi University, China 2008 Outstanding Student, Guangxi University, China 2007 A third place in the 3th "Challenge Cup" Guangxi College Student Curricular Academic Science and Technology Works Competition, Guangxi, China 2007 Student Award for Research and Innovation, Guangxi University, China RESEARCH SKILLS Laboratory Skills and Techniques: Molecule biology D/RNA extraction, Molecular cloning, DNA vector construction, Western blot, Real time qrt-pcr, ChIP-qPCR. Microarray hybridization, Sequencing library preparation, single cell RNA-seq, single cell RRBS, single cell WGBS. shrna mediated gene knockdown, TALEN and CRISPR mediated gene editing. Cell biology Cell culture, Viral preparation, and Somatic cell reprogramming, FACS analysis. Generation and culture of mouse ipscs, Characterization of mouse ipscs or ESCs including Embryoid Body (EB) and teratoma formation, Alkaline Phosphatase (AP) and Immunostaining. Embryology In vitro fertilization, In vitro culture, and Embryo grading in cattle. Analytical Skills: Statistical programs including Excel, Minitab and SAS. Bioconductor statistical packages in R statistical environment for microarray and RNA- 3
seq data analysis. GeneSpring for analyzing qrt-pcr, Microarray and RNA-seq data, BaseSpace for analyzing Illumina RNA-seq data. Biological databases and genetic analytical programs including DAVID, KEGG and IPA, GenBank, Human Genome Project database and BLAST. PUBLICATIONS NCBI Submissions: GSE75559: Jiang Z, Harrington P, Zhang M, Marjani SL, Park J, Kuo L, Pribenszky C, Tian X. Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts. Scientific Reports, Jan 2016 Accepted. GSE59186: Jiang Z, Sun J, Dong H, Luo O, Zheng X, Obergfell C, Tang Y, Bi J, O'Neill R, Ruan Y, Chen J, Tian XC. Transcriptional Profiles of Bovine In Vivo Preimplantation Development. BMC Genomics. 2014 Sep 4;15:756.. GU477630: Jiang Z, Zhang M, Lin Y, Zhu J, Li M, Luo Y and Gan X. Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Oreochromis Aureus. Journal of Fishery Sciences of China. 2011, 18(4):790-800. GU477624, GU477625, GU477626, GU477627, GU477628, GU477629, and GU477631: Jiang Z, Gan X, Zhang M, Lin Y, Zhu J, and Li M. Complete Mitochondrial Genome Sequence of Oreochromis niloticus, Oreochromis Aureus, and Oreochromis sp, 2010, (Direct Submission, unpublished). Peer-reviewed publications: 1. Jiang Z, Harrington P, Zhang M, Marjani SL, Park J, Kuo L, Pribenszky C, Tian X. Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts. Sci Rep. 2016 Feb 17;6:21215. doi: 10.1038/srep21215. 2. Jiang Z, Tang Y, Zhao X, Zhang M, Blomberg Le Ann, Tian XC. Loss of Brm and Baf170, Components of Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells. Stem Cells Dev. 2015 Oct 1;24(19):2328-36. doi: 10.1089/scd.2015.0069. Epub 2015 Jun 29. 3. Jiang Z, Sun J, Dong H, Luo O, Zheng X, Obergfell C, Tang Y, Bi J, O'Neill R, Ruan Y, Chen J, Tian XC. Transcriptional Profiles of Bovine In Vivo Pre-implantation Development. BMC Genomics. 2014 Sep 4;15:756. (Highly accessed). 4. Jiang Z, Dong H, Zheng X, Marjani S, Donovan D, Chen J,and Tian XC. Transcriptional Profiles of Imprinted Genes during Bovine In Vivo Embryo Development. Sci Rep. 2015 Dec 7;5:17898. doi: 10.1038/srep17898. 5. Sun J*, Jiang Z*, Tian XC, Bi J. A Cross-Species Bi-Clustering Approach to Identifying Conserved Co-regulated Genes. Bioinformatics, 2016 (*Co-first author) 6. Tang Y, Jiang Z, Luo Y, Zhao X, Wang L, Norris C, Tian XC. Differential Effects of Akt Isoforms on Somatic Cell Reprogramming. J Cell Sci. 2014 Sep 15;127 (Pt 18):3998-4008. 7. Tang Y, Luo Y, Jiang Z, Ma Y, Lin CJ, Kim C, Carter MG, Amano T, Park J, Kish S, and Tian XC. Jak/Stat3 Signaling Promotes Complete Reprogramming by Epigenetic 4
Regulation. Stem Cells. 2012 Dec;30(12):2645-56. Peer-reviewed publications in Chinese journals 8. Jiang Z, Zhang M, Lin Y, Zhu J, Li M, Luo Y and Gan X. Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Oreochromis Aureus. Journal of Fishery Sciences of China. 2011, 18(4):790-800. 9. Zhu J, Chen S, Bi J, Jiang Z, Luo Y, Ma H, Gan X. Genetic Diversity between Sexes of Genetic Improvement of Farmed Tilapia Reveal by SSR Analysis. Genomics and applied biology. 2010, 29(1):1117-1120. 10. Jiang Z, Zhang M, Fu Q. Study on Expression Characteristics of Mouse Gonad-specific Expression Gene (GSE). Southwest China Journal of Agriculture Science. 2009, 22 (4):1114-1117. 11. Qin W, Jiang Z, Zhang M. Study Progress on Gonad-specific Expression Gene in Mammalian. Guangxi Agricultural Science. 2009, 40 (9): 1210-1214. Manuscripts in review: 1. Paula Tribulo, James I Moss, Manabu Ozawa, Zongliang Jiang, Xiuchun (Cindy) Tian, and Peter James Hansen. Developmental Changes in Canonical WNT Signaling in the Preimplantation Bovine Embryo. Biology of Reproduction. 2016 2. Yong Tang, Ling Wang, Delun Huang, Zongliang Jiang, Xiuchun Tian. Akt3 Is Responsible for the Survival and Proliferation of Embryonic Stem Cells. Stem cells and Dev. 2016 Manuscripts in Preparation: 1. Jiang Z, Sun J, Tang Y, Bi J Chen J, and Tian XC. Comparative Analysis of RNA Sequencing Data from Pre-implantation Embryonic Development of Mammalian Species. 2. Jiang Z, Sun J, Chen J, and Tian XC. Comparative Analysis of Transcriptional Profiles of Bovine Pre-implantation Embryos Derived from In Vivo and In Vitro. 3. Jiang Z, Zhang M, Chen J, and Tian XC. Quantification of Housekeeping Transcript Levels during Bovine In Vivo Preimplantation Embryos. 4. Duan J, Jiang Z, Nathaniel J, O Neill R, Wolf E, Blomberg LA, Talbot N, Dong H, Zheng X, Chen J, and Tian XC. Incomplete compensatory up-regulation of X-linked gene in bovine germline cells, pre-, peri- implantation embryos, and somatic cells. 5. Zhu L, Jiang Z, Chen J, and Tian XC. Messenger RNA abundance of histone variants, histone and DNA modification enzymes in bovine in vivo oocytes and pre-implantation Embryos. 6. Rashid Ali, Jiang Z, Chen J, and Tian XC. Transcriptional Profiles of Genes Regulated Mitochondrial during Bovine In Vivo Emryo Development. Poster Presentations: 1. Jiang Z, Tian XC. Effects of high hydrostatic pressure on expression profiles of in vitro produced, vitrified bovine blastocysts. (42 th Annual conference of IETS, Louisville, Kentucky, U.S.A, Jan, 2016) 2. Duan J, Jiang Z, Nathaniel J, O Neill R, Wolf E, Blomberg LA, Talbot N, Dong H, 5
Zheng X, Chen J, and Tian XC. Dosage Compensation and X-linked Gene Expression in Bovine in vivo and in vitro Embryos. (42 th Annual conference of IETS, Louisville, Kentucky, U.S.A, Jan, 2016) 3. Jiang Z, Tang Y, Tian XC. (2015) Loss of Brm and Baf170, Components of the ATPdependent Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells, Reproduction, Fertility and Development, 2014, 27 (1) 260-261. (41 th Annual conference of IETS, Paris, France) 4. Jiang Z, Tang Y, Tian XC. (2014) Loss of Brm and Baf170, Components of the ATPdependent Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells. (11 th Annual Conference of NYSCF, NY, USA) 5. Jiang Z, Sun J, Tian XC. (2014) Complete Transcriptional Profiles of Bovine In Vivo Pre-implantation Development. (CANR Graduate Research Forum, Storrs, CT) 6. Tang Y, Luo Y, Jiang Z, Zhao X, Tian XC. (2013) Stat3 Epigenetically Controls Pluripotency Establishment during Mouse ipsc Induction. (ISSCR, Boston, MA) 7. Tang Y, Luo Y, Jiang Z, Carter M, Kish S, Tian XC. (2013) Jak/Stat3 Signaling Promotes Complete Reprogramming by Epigenetic Regulation. (39th Annual Conference of IETS, Hannover, Germany) 8. Tang Y, Luo Y, Jiang Z, Zhao X, Tian XC. (2013) Stat3 Epigenetically Controls Pluripotency Establishment during Mouse ipsc Induction. (StemConn2013, CT) Seminar Presentations: 1. Bovine Pre-implantation Embryo development and Epigenetic Regulation during Somatic Cell Reprogramming. (Invited Speaker, Xinjiang Academy of Animal Science, Urumchi, Xinjiang, China, Feb 6, 2015) 2. Complete Transcriptional Profiles of Bovine In Vivo and Pressure-treated Embryos. (Invited Speaker, Guangxi University, Nanning, Guangxi, China, Feb 2, 2015) 3. Transcriptional Profiles of Bovine In Vivo and Pressure-treated Embryos, and Roles of BAF Complex in Reprogramming. (UCONN ANSC Ph.D Dissertation Proposal Defense, Storrs, CT, May 8, 2014) 4. Loss of Brm and Baf170, Components of the ATP-dependent Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells. (UCONN ANSC Seminar Series, Storrs, CT, April 25, 2014) 5. Complete Transcriptional Profiles of Bovine In Vivo Pre-implantation Development. (UCONN ANSC Seminar Series, Storrs, CT, Nov 1, 2013) 6
ACADEMIC AND PERSONAL REFERENCES 1. Prof. Xiuchun (Cindy) Tian, Professor of Animal Science, Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA. Email: xiuchun.tian@uconn.edu Phone: 860-486-9087 Fax: 860-486-0534 2. Prof. Emre U Seli, MD Professor, Obstetrics, Gynecology & Reproductive Sciences; Egg Donation and Gestational Surrogacy Program Yale University, School of Medicine New Haven, CT 06519, USA Emre.seli@yale.edu Phone: 203-785-7873 Fax: 203-785-7819 3. Prof. Ming Zhang, Professor of Animal Science, Animal Reproduction Institute, Guangxi University, Guangxi, Nanning, P.R. China. Email: mingzhang@gxu.edu.cn Phone: +86-771-3234441 Fax: +86-771-3234441 7