RESEARCH NEWS. Perfect for athletes and health aficionados: the lupine protein beverage

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August 2017 Page 1 5 Perfect for athletes and health aficionados: the lupine protein beverage The beverage market in the health, sports and wellness sector is continually coming up with new products. Now, researchers at Fraunhofer have developed a protein-rich drink based on sweet lupines, which tastes sour and refreshing at the same time. And the lupine drink can be produced at any brewery. With its intensive colors and many blossoms, the lupine looks like an ornamental plant. Yet, the tall lupine is far too good to be used decoratively as the plant s seeds contain nutritious proteins. However, it is rather complicated to make lupines edible for humans. Researchers at the Fraunhofer Institute for Process Engineering and Packaging IVV have now developed a process that uses the lupine seeds as a basis for a protein-containing beverage with a refreshing and sour taste. The institute of brewing and beverage technology at the Technical University of Munich functioned as a cooperation partner and was involved in the project. During the development process, the researchers had to overcome several hurdles. In particular, it is a real challenge to create a refreshing drink with proteins, as they generally are not soluble in the acidic ph range. Lupines are an exception here, since they contain a type of protein that can also be dissolved in the acidic ph range. However, the plant belonging to the legume family, which also include beans, peas and peanuts presented the scientists with a different problem: like all the above-mentioned legumes, it contains phytic acid. This compound binds valuable minerals and inhibits enzymes and is therefore difficult to digest. The protein remains intact, the phytic acid is degraded The researchers at Fraunhofer have now developed a process that preserves the sweet lupines nutritious proteins and reduces the undesirable phytic acid during processing. The scientists rely on a combination of malts and special microorganisms. The latter degrades the phytic acid hydrolytically; with the help of water, so to say. A two-stage mashing and fermentation process is used. The researchers have optimized and adapted this relatively uncomplicated nonetheless delicate process in several places. The Contact Janis Eitner Fraunhofer-Gesellschaft, München Communications Phone +49 89 1205-1333 presse@zv.fraunhofer.de Karin Agulla Fraunhofer Institute for Process engineering and Packaging IVV Phone +49 8161 491-120 Giggenhauser Strasse 35 85354 Freising www.ivv.fraunhofer.en karin.agulla@ivv.fraunhofer.de

result: a lupine extract in form of a paste or powder. This is then the starting point for a wholesome, nutritious and refreshing beverage. Moreover, by using the extract as the basic ingredient for the beverage, the protein content can be adjusted as needed. The lupine extract has a relatively neutral taste, which allows for a wide variety of taste options. August 2017 Page 2 5 The lupine drink is a good example of how the Fraunhofer IVV works. "We develop and optimize manufacturing processes, combining traditional methods with new ingredients. This allows us to create new, healthy and sustainable foods," explains Raffael Osen, project manager at Fraunhofer IVV. A process similar to brewing beer Anyone who thinks of beer when hearing the terms of malt, mash and fermentation is on the right track. The manufacturing process is similar to beer brewing and uses devices like mashing pans, lauter tuns or fermenting tanks, which every brewery has. Additional large investments are therefore not required. "Even small breweries are able to put health, sports, and wellness beverages on the market at a low economic risk." Refreshing as a cold beer, rich in proteins and 100% alcohol free. This lupine beverage is the ideal drink after sports a refreshing, low calorie and tasty drink straight out of the fridge. It is comparable to a cool whey drink that is carbonated, but is plant-based and thus lactose-free and vegan. Ecologically conscious consumers can also enjoy this lupine drink without feeling guilty, because the vegetable raw materials are from local farmers. Legumes as a refreshment beverage The sweet lupine is not the only plant that Fraunhofer researchers are focusing on. Other legumes such as beans and peas could serve as the basis for healthy and proteinrich beverages, too. The manufacturing processes have to be adapted only slightly. "The process is now well established. In the next step, we are trying to apply the process to other raw materials in order to achieve greater product diversity, "says Mr. Osen, project manager. "Especially regional protein-rich plants such as peas or beans have great potential." As commercial partners, the companies Wild and Döhler have contributed their knowhow to the project. Wild, a company from Heidelberg, Germany, is a specialist for beverage ingredients and has already produced a drink with mango and apple flavor based on the lupin extract. Döhler supported the project through their expert advice as well as the analysis of intermediate and final products.

Regional breweries such as the Riedenburger brewery and the Lammsbräu brewery from Neumarkt, Germany, have also accompanied the project with their expertise. The project is funded by the German Federal Ministry of Economics and Energy and the Allianz Industrial Research. August 2017 Page 3 5 However, those who come up with the idea of snacking on sweet lupine during a summer hike must be warned. Wildly growing lupines contain toxic bitter substances. It is better to wait for the first lupine drink from the local brewery. Lupines A leguminous plant from the subfamily of the papilionoïds. There are different varieties, including sweet lupins, yellow and blue lupines. The wild-growing plants are poisonous and especially dangerous for people suffering from allergies. Only non-toxic breeding lupines are used for food production. The lupine seeds are rich in proteins and are therefore suitable as a basis for manufacturing food. Malting The germination process in which grains are converted into malt. Mashing During this process, raw materials are mixed with yeasts and water is added. The starch in potato or cereal turns into sugar, which is converted into alcohol. Fermentation Organic substances are mixed with either bacteria, yeasts or fungi, allowing an enzymatic process to be set into motion.

August 2017 Page 4 5 This plant is not suitable for consumption when found in the wild, yet cultivated lupines supply high-quality protein and can therefore serve as the basis for healthy refreshing beverages. Fraunhofer IVV Picture in color and printing quality: www.fraunhofer.de/en/press

August 2017 Page 5 5 A protein-rich yet refreshing smoothie for the summer: the extract of the sweet lupine can be made into a great variety of beverages. Fraunhofer IVV Picture in color and printing quality: www.fraunhofer.de/en/press. The Fraunhofer-Gesellschaft is the leading organization for applied research in Europe. Its research activities are conducted by 69 institutes and research units at locations throughout Germany. The Fraunhofer-Gesellschaft employs a staff of 24,500, who work with an annual research budget totaling 2.1 billion euros. Of this sum, 1.9 billion euros is generated through contract research. More than 70 percent of the FraunhoferGesellschaft s contract research revenue is derived from contracts with industry and from publicly financed research projects. International collaborations with excellent research partners and innovative companies around the world ensure direct access to regions of the greatest importance to present and future scientific progress and economic development.