Silage Hybrid testing at Penn State. Penn State testing program. Overall goals of our program

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Silage Hybrid testing at Penn State Moving Forward with Silage Hybrid Evaluation GREG ROTH DEPARTMENT OF PLANT SCIENCE Began evalutions in mid 1990 s Sabbatical leave at Miner Institute in 2000 Revamped Penn State program in 2001 Steady growth through the years Sabbatical in 2016 to visit other programs Penn State testing program Partner with Professional Dairy Managers of Pennsylvania Provide input w/advisory team Solicit hybrids Develop initiatives Overall goals of our program Test hybrids Improve Characterization Methods Broaden Collaborations Develop Funding for Assessments Provide Roadmap for the Future

Laboratory Partner Provides insights into analytical issues and trends in the industry Help to work toward some consistency in evaluation and interpretation Leadership Team Provides insights into analytical issues and trends in the industry Helps to work toward some consistency in evaluation and interpretation Chris Canale Alex Hristov Jess Williamson Evaluation Program Principles Replicated, multiple environment evaluations Sort hybrids by DM within test Provide maturity subcategories at each location Conduct tests under typical crop conditions Conduct separate test for BMRs Report data to producers by November 1 2016 PDMP/PSU Silage Hybrid Evaluation Program Evaluated 163 hybrids 88 94 d RM (4) 95 103 d RM (20) 98 111 d RM (40) 104 111 RM/SC PA (33) 111 115 d RM (36) 116 120 d RM (16) Nine locations Blair (2), Bradford (2), Cambria, Centre, Franklin, Lancaster (2), and Chester Counties Three replications at each site Separate BMR evaluation at 3 locations (5)

PSU Data Presentation can be found at: http://cornandsoybeans.psu.edu/ Table Advisory Board Inputs Do hybrids vary in starch digestibility? Should we be evaluating fiber digestibility in more detail such as undf? Are there alternatives to Milk 2006? Production Details / Plot Background

Sabbatical II 2015/16 Visited other silage testing programs across US Discussed current procedures and issues In NY, visited Miner and Cornell Cornell was rebooting their program Collaboration with Cornell Strive for similar field and analytic procedures Work on incorporation of advanced fiber measurements Evaluate starch digestibility Develop alternative interpretation system Work with UVM and WNY Crop Mgmt Assn. 2016 NYS Corn Silage Hybrid Trials Producer and Industry Support led to re establishing the Commercial Corn Silage Hybrid Testing Program Collaboration of PRO DAIRY, Department of Animal Science, Cornell University Section of Plant Breeding & Genetics, Cornell University With support from NYS Dairy Producers Seed Corn Industry NYS Agricultural Experiment Station Cornell Cooperative Extension NY Farm Viability Institute Joe Lawrence A Special Thank You Tom Overton Margaret Smith Mike Van Amburgh Jerry Cherney Bill Cox Allison Lawton Sherrie Norman Keith Payne Dan Fisher Jon Greenwood Paul Stackowski Fiber assessment undf 240 better describes indigestibility of the forage for use in cattle Improves prediction of energy from forages Helps improve predictions of intake and rumen function, microbial production etc. Can help explain performance issues with fiber digestibility in some cases NIRS prediction available from CVAS

32.0 undf variation over locations undf (% NDF) variation 33 hybrids, 2016 2016 Penn State undf 240 vs NDFD 31.0 LSD 0.10 30.0 29.0 28.0 27.0 26.0 25.0 NDFD 24 hr, %NDF 2016 Penn State undf 240 vs NDFD 2016 NYS Corn Silage Trials NDFd 24 hr, %NDF Take home: Large range in undfs across all of our tests. 8 point diff @ NDFD 55 Smaller range within a test or location Relationship between undf and NDFD varies depending on locations Blue points in lower right are BMRs under drought stress in Juniata Co.

MADRID AURORA Slide Credit: Dr. Randy Shaver Fiber Conclusions Continue with the development of undf 240 as a routine measurement for silage assessment. Standardize our reporting as %NDF Change NDFD 24 hour to NDFD 30 at Penn State to be consistent with Cornell to facilitate comparisons. Add NDFD 120 and NDFD240 to reports Starch Digestibility Hybrids with more floury endosperm are being marketed with the potential to improve starch digestibility How can differences be best assessed? How do differences interact with other factors such as particle size and time of ensiling?

Starch Digestibility Assessment Study Objective: characterize starch digestibility of hybrids over time. 6 hybrids Check: TA 780, H5609 Test: HHG 7412, HHG 864, H5811, H5505 2 locations x 3 replicates x 5 time Time = 0, 60, 120, 180, 240d Grind: 4 and 1mm CVAS: 7h IVSD wet chemistry Starch digestibility over ensiling time Starch digestibility by hybrid variety In situ vs. In vitro In situ vs. NIR In situ vs. In vitro In situ vs. NIR Digestibility, % Starch 100.0 95.0 90.0 85.0 80.0 75.0 70.0 65.0 60.0 55.0 0 30 60 120 150 Days Ensiled In situ starch deg. 8 h, % starch (Lab 4) In situ soluble starch, % starch (Lab 4) In vitro starch dig. 7 h, % starch (Lab 3) In vitro starch dig. 7 h, % starch (Lab1) Digestibility, % Starch 100.0 95.0 90.0 85.0 80.0 75.0 70.0 65.0 60.0 55.0 0 30 60 120 150 Days Ensiled In situ starch deg. 8 h, % starch (Lab 4) NIR starch dig. 7 h, % starch (Lab 1) NIR starch dig. 7 h, % starch (Lab 2) In situ soluble starch, % starch (Lab 4) Digestibility, % Starch 95.0 90.0 85.0 80.0 75.0 70.0 65.0 60.0 b 42HFC15 a H5222RC3P c c c H5333RC3P H6191RCSS MC 5250 Hybrid In vitro starch dig. 7 h, % starch (Lab1) In vitro starch dig. 7 h, % starch (Lab 3) In situ starch deg. 8 h, % starch (Lab 4) In situ soluble starch, % starch (Lab 4) Digestibility, % Starch 95.0 90.0 85.0 80.0 75.0 70.0 65.0 60.0 y b 42HFC15 y a H5222RC3P x y c c H5333RC3P H6191RCSS Hybrid y c MC 5250 NIR starch dig. 7 h, % starch (Lab 1) NIR starch dig. 7 h, % starch (Lab 2) In situ starch deg. 8 h, % starch (Lab 4) In situ soluble starch, % starch (Lab 4)

G3/4 Combined Data, 0h IVSD7 Starch Digestibility: Conclusions I V S D 7 DM, % SE=1.5 Tests are reasonably consistent at detecting large differences due to ensiling time Less consistent detecting smaller hybrid differences Hybrid differences most apparent at 0 and 30 hours Current plan is to assess hybrids this fall with wet chem 7 h IVSD with a 1mm grind on 5 replicated studies Interpretation methods We have used Milk/ton based on the Milk 2006 spreadsheet developed by Univ of WI to interpret yield and quality data Advisors express concern about value of fiber digestibility in Milk/ton ratings. New advances in understanding of forage quality New laboratory analyses becoming available

Corn Picker A model based approach for 2016 and beyond Predicting Milk Yield Utilizing Cornell Net Carbohydrate and Protein System v. 6.5.5 What is CNCPS? Dynamic model used to balance feed rations for cattle Under continuous development since 1980 s First applied to on farm use in the early 1990 s Currently used as the basis for ration software programs that feed more than 40% of the cows in the US and many more cows worldwide. Predicting Milk Yield Utilizing Cornell Net Carbohydrate and Protein System v. 6.5.5 (NDS platform) Base diet formulated for an ME & MP allowable milk yield of 100 lbs/day 2 nd lactation, 110 DIM, 1585 BW, 3.80% milk fat, 3.20% milk true protein High Corn Silage Diet 28 lbs DM from Corn Silage ~60% Forage in Ration Target: 60 lbs Dry Matter Intake (DMI) CNCPS Feed Library Values Used for base ration NDF digestibility values from 2 year average in Cumberland Valley Analytical Services database for: Corn Silage, Alfalfa Haylage, Straw Predicting Milk Yield Utilizing Cornell Net Carbohydrate and Protein System v. 6.5.5 (NDS platform) Calculations Predicted ME Allowable Milk Yield for each hybrid using same Dry Matter Intake (DMI) as the base ration Library value for starch digestibility decision for nutrition group due to uncertainty of green samples Replaced Corn Silage (on dry matter basis) in base ration with each hybrid in the trial Calculate expected DMI undf240 equivalent of the base ration Calculate undf240 intake on a DMI equivalent

Predicting Milk Yield Utilizing Cornell Net Carbohydrate and Protein System v. 6.5.5 (NDS platform) undf240 Adjustment Predicted ME Allowable Milk undf240 equivalent (lbs/day) Adjusted total ration DMI so the undf240 was equal to that in the base ration. Reflects how much the cow actually may be able to consume based on rumen fill. Aurora Relative Maturity 35% DM Dry Matter Predicted ME Allowable Milk undf240 Intake, Adjusted TMR DMI, Predicted ME Allowable Milk undf240 tons/acre % lbs/day lbs/day lbs/day lbs/day CNCPS value CNCPS value CNCPS value CNCPS value 84 95 day 15.3 31.4 107.2 5.3 66.4 121.6 96 100 day 17.5 32.4 107.7 5.2 67.3 124.2 101 107 day 19.8 34.5 107.6 5.5 63.7 116.3 Madrid Relative Maturity 35% DM Dry Matter Predicted ME Allowable Milk undf240 Intake, Adjusted TMR DMI, Predicted ME Allowable Milk undf240 tons/acre % lbs/day lbs/day lbs/day lbs/day CNCPS value CNCPS value CNCPS value CNCPS value 84 95 day 27.6 34.6 103.5 6.4 54.8 93.3 96 100 day 28.3 33.9 104.8 6.1 57.5 100.1 101 107 day 29.3 31.9 104.5 6.2 57.3 99.3 Impact of undfd on predicted milk

Interpretation: Conclusions Model based approach has merit and can incorporate more advanced measurements Need to do more validation of intake and milk differences with animal data Need a method to adapt to more of a spreadsheet format to facilitate data processing NDFd 24 hour % BMR Study: NDFD 75 70 65 60 55 50 BMR10B27RA P1180XR BMR12B75 45 40 P1449AMX BMR14B96 Chester Lancaster Juniata BMR Study: undf 29 BMR10B27RA P1180XR 27 25 BMR12B75 High NDFD, Medium undf P1449AMX BMR14B96 23 21 High NDFD, Low undf 19 17 15 Chester Lancaster Juniata Summary There is a need for some updating in our assessment of hybrids to reflect current issues in dairy nutrition Some development of approaches is necessary and underway Working together we hope to develop some consistency and better collaboration Testing programs can be more than just hybrid evaluation