Beta Amyloid Peptides

Beta Amyloid Peptides The Most Comprehensive Collection for Alzheimer s Disease Academic Services Pharmaceutical Services

Beta Amyloid Peptides The Most Comprehensive Collection for Alzheimer s Disease Proprierary FlexPeptide TM technology with combined platform, microwave and ligation system used for amyloid peptide synthesis. igher quality with less expensive price. Superior Purity All products purity. Rigorous QC Test Products must pass extensive quality control, such as PLC, MS, etc. Wide Selection ver 59 amyloid products available. We also provide customized peptides on special request. Quick rdering System All products are in stock and promptly shipped following purchase. State-of-The-Art Facilities GMP What are Beta Amyloid Peptides Beta amyloid is a protein fragment that forms amyloid plaques in the brain of Alzheimer's disease (AD) patients. Beta amyloid peptide consists of 43 amino acids. It is created via the enzymatic digestion of amyloid precursor protein (APP) by beta secretase and gamma secretase. It then forms insoluble fibrous aggregates, causing amyloidosis and neurodegenerative diseases such as AD. Due to beta amyloid's pathology, it has become one of the most investigated molecules in Alzheimer's disease research. Why choose our Beta Amyloid Peptides GenScript's FlexPeptide TM system and click peptide platform allows us to synthesize a comprehensive array of human and rat beta amyloid peptide fragments. Testing these fragments can help elucidate the dynamic biological interactions of beta amyloid peptide and facilitate a better understanding of Alzheimer s disease at the molecular level. At GenScript, we are dedicated to providing the most current and highest quality reagents in the global effort to treat and find a cure for Alzheimer s disease. With our philosophy of always striving to be on the forefront of new technology and its application in treating disease, you can be confident you have a committed partner to further your research and future innovations. Advanced Technologies 1. FlexPeptide TM Technology includes: a. Combined platform GenScript has several LPPS and SPPS methods that ensure high peptide synthesis success rates. b. Microwave technology The smart and efficient way to optimize peptide synthesis yield c. Ligation technology Long peptides up to 200 aa can be synthesized. 1 www.genscript.com

Case study : β-amyloid (1-40) Sequence: Asp - Ala - Glu - Phe - Arg - is - Asp -.........- Leu - Met - Val - Gly - Gly - Val - Val Length: 40 aa Purity: 95.55% PLC results mv 300 Chromatogram 200 16.287 0 15.288 16.833 16.650-0 5 10 15 20 25 30 35 min 1 Det.A Ch1 / 220nm MS results Positive Intensity 90 80 Mass Spectrum 1083.65 [M+4]4+ 70 60 50 [M+a+4]5+ 871.50 40 30 20 10 726.55 [M+3]3+ 1444.40 400 500 600 700 800 900 0 1 1200 1300 1400 1500 1600 1700 1800 1900 m/z www.genscript.com 2

Case study III: β-amyloid (25-35) Sequence: Gly - Ser - Asn - Lys - Gly - Ala - Ile - Ile - Gly - Leu - Met Length: 11 aa Purity: 95.18% PLC results mv 400 Chromatogram 17.632 300 200 0 15.422 16.367 16.783 17.041 17.426 17.281 19.234 20.279 20.454 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 min Det.A Ch1 / 220nm MS results Positive Intensity 90 80 70 60 50 530.95 [M+2]2+ Mass Spectrum 40 30 20 [M+]+ 1060.40 10 300 400 500 600 700 800 900 0 1 1200 1300 1400 1500 1600 1700 1800 1900 m/z 3 www.genscript.com

2. Click Peptide Platform The preparation of hydrophobic peptides like beta-amyloid can be a time consuming process. Even after peptides are successfully prepared, proper dissolving of peptides is critical. At GenScript, our featured click peptide platform is specially designed to handle the low water-solubility and aggregative nature of hydrophobic peptides. (Xaa) m 2 (Xaa) n Xaa: amino acid - intramolecular acyl migration p 7.4 ne way and quick process (Xaa) m (Xaa) (ydrophilic, chick peptide) - acyl lsopeptide (ydrophobic, native peptide) A click peptide based on the -acyl isopeptide method Case Study : 26--acyl β-amyloid (1-42) click peptide was successfully synthesized. The β-ester bond was quickly and quantitatively converted to a native Gly25-Ser26 amide bond via a p-dependent - intramolecular acyl migration reaction (t1/2 =1 min, p 7.4, 37 ) at a hydroxyamino acid residue. amely, upon this p-triggered conversion (p-click), the non-aggregative and water-soluble precursor (click peptide) can produce the monomer with a random-coil structure under physiological conditions (p 7.4, 37 ). The structure information is as follows. Aβ1-24 2 Aβ27-42 -to- acyl migration p 7.4 ne way and quick process Aβ1-24 Aβ27-42 26--acyl isoaβ 1-42 (click peptide) amyloid β peptide (Aβ) 1-42 β-amyloid (1-42) click peptide Results β-amyloid precursor(click peptide) has a water solubility of 1/ml, while it is only 0.14 mg/ml for the native peptide. The aggregative property of the peptide is significantly reduced. The -acyl moiety was stable under acidic p. www.genscript.com 4

The following are PLC reports of purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor) and purified 26--acyl β-amyloid (1-42) click peptide (precursor). mv Chromatogram 19.154 150 50 0 16.329 18.250 18.717 22.114 1 Det.A Ch1 / 220nm Detector A Ch1 220nm Peak# 1 2 3 4 5 Total 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 Ret. Time 16.329 18.250 18.717 19.154 22.114 Area 25804 57359 51643 4094569 76280 4305656 Peak Table eight 4398 4489 3482 180301 8679 201349 Area % 0.599 1.332 1.199 95.097 1.772.000 PLC report of purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor) min mv Chromatogram 400 18.627 300 200 0 9.684 18.985 23.524 1 Det.A Ch1 / 220nm 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 min Detector A Ch1 220nm Peak# 1 2 3 4 Total Ret. Time 9.684 18.627 18.985 23.524 Area 65 3071633 133310 14301 3229309 eight 2143 411091 11373 1914 426521 Area % 0.312 95.117 4.128 0.443.000 5 www.genscript.com

rdering List: Category Cata o. Purity ame Size RP02-0.5 β-amyloid (1-28) RP04-0.5 β-amyloid (1-40) RP04-1 β-amyloid (1-40) RP08-1 β-amyloid (25-35) RP08-5 β-amyloid (25-35) RP13-0.5 β-amyloid (1-42), rat RP13-1 β-amyloid (1-42), rat RP16-0.5 β-amyloid (1-40), rat RP16-1 β-amyloid (1-40), rat RP17-0.5 β-amyloid (1-42), human RP17-1 β-amyloid (1-42), human RP34-0.5 β-amyloid (42-1) RP34-1 β-amyloid (42-1) RP20101 β-amyloid (1-33) RP20102 β-amyloid (11-22) RP20103 β-amyloid (1-16) RP20104 β-amyloid (12-28) RP20105 β-amyloid (18-28) RP20106 β-amyloid (1-11) RP20130-1 β-amyloid (22-35) RP20130-5 β-amyloid (22-35) RP20138 β-amyloid (1-37) RP20139 β-amyloid (1-36) RP20140 β-amyloid (1-34) RP20141 β-amyloid (1-15) RP20142 β-amyloid (26-40) RP20143 β-amyloid (30-34) RP20144 β-amyloid (33-40) RP20145 β-amyloid (35-42) RP20146 β-amyloid (8-38) RP20148 β-amyloid (17-24) www.genscript.com 6

Category Cat o Purity ame Size RP20150 β-amyloid (22-40) RP20152 β-amyloid (16-23) RP20153 β-amyloid (16-24) RP20154 β-amyloid (16-26) RP20160 β-amyloid (1-9) RP20161 β-amyloid (1-17) RP20162 β-amyloid (1-20) RP20163 β-amyloid (10-35) RP20165 β-amyloid A4 Protein Precursor (740-770), APP (C31) RP20166 β-amyloid (1-40) Binding Peptide RP20168 Cys Containing β-amyloid (1-40) Binding Peptide RP20169 Parallel topology β-amyloid modified peptide RP20170 β-amyloid (13-27) RP20171-1 β-amyloid (15-21) RP20171-5 β-amyloid (15-21) RP20172-1 β-amyloid (16-20) RP20172-5 β-amyloid (16-20) RP20173-1 β-amyloid (4-10) RP20173-5 β-amyloid (4-10) RP20174 β-amyloid (10-20) RP20175 β-amyloid (12-20) RP20177-1 β-amyloid (32-35) RP20177-5 β-amyloid (32-35) RP20178-1 β-amyloid (29-40) RP20178-5 β-amyloid (29-40) RP20184-1 β-amyloid (31-35) RP20184-5 β-amyloid (31-35) 7 www.genscript.com

Category Cat o Purity ame Size RP20185-0.5 β-amyloid (35-25) RP20185-1 β-amyloid (35-25) RP20185-5 β-amyloid (35-25) RP20186 β-amyloid (37-43) RP20192 β-amyloid (7-22) RP20195 β-amyloid Protein Precursor (657-676) RP20196-1 β-amyloid Protein Precursor 770 (135-155) RP20196-5 β-amyloid Protein Precursor 770 (135-155) Mca-(Asn 670,Leu 671 )-Amyloid RP20207 β/a4 Protein Precursor770 (667-675)-Lys(Dnp) Mca-(Asn 670,Leu 671 )-Amyloid RP20208 β/a4 Protein Precursor770 (667-675)-Lys(Dnp), amide RP20265 Beta-Amyloid (10-25) RP20266 Amyloid Precursor Protein (APP) (751-770) RP20278 Beta-Amyloid (36-42) RP20352 Beta-amyloid peptide (1-8) RP20367 Beta-amyloid(33-42) RP20405 Beta-Amyloid (11-17) RP20483 Cys-Beta-Amyloid (25-35) RP20505 Beta-Amyloid(1-14),mouse,rat RP20527-0.5 β-amyloid (1-42), human RP20527-1 β-amyloid (1-42), human RP20528-0.5 β-amyloid (1-40) RP20528-1 β-amyloid (1-40) GenScript offer customized bulk synthesis, please send your request via product@genscript.com For current price, visit http://www.genscript.com/beta_amyloid_peptide.html For related amyloid antibodies, visit http://www.genscript.com/antibody-list/a/1.html www.genscript.com 8

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