Roc LC s Conventional column built to be the cornerstone for your lab; pressure rated for any 400 bar system. Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you demand. Exceptional value for routine analyses and large-volume workflows high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most methods. Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods. Newly designed minimal packaging for compact storage download serial #-specific certificates of analysis at www.restek.com/documentation Get more of what you want, and less of what you don t, with Roc LC columns from Restek. Order today at www.restek.com/roc Roc Family of LC Phases C8 C8 Phenyl-Hexyl Cyano Silica C N OH CH 3 Si CH 3 O CH 3 Si CH 3 O CH 3 Si CH 3 O CH 3 Si O CH 3 Si O O O C8 Cyano Phenyl-Hexyl USP Phase Code L L7 L L0 L3 Stationary Phase Category C8, octadecylsilane C8, octylsilane phenyl cyano bare silica Ligand Type monomeric C8 monomeric C8 phenyl-hexyl cyanopropyl n/a Particle Size 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical Pore Size 00 Å 00 Å 00 Å 00 Å 00 Å Surface Area 300 m /g 300 m /g 300 m /g 300 m /g 300 m /g Carbon Load 0% % 5% 8% n/a End-Cap yes yes yes yes n/a ph Range.5 to 8.0.5 to 8.0.0 to 8.0.5 to 8.0.5 to 8.0 Maximum Temperature 80 C 80 C 80 C 80 C 80 C www.restek.com
Roc On with These Dependable LC s Figure : Over,000 injections and Roc columns will still offer reliable performance, regardless of which dimension your method calls for. 04 0 00 98 96 94 9 % Efficiency vs # of Injections Roc 5 µm C8s @ 375 bar 90 0 50 500 750 000 00 x 4.6 mm 50 x 4.6 mm 50 x 4.6 mm 00 x 3.0 mm 50 x 3.0 mm 50 x 3.0 mm Figure : Add a Roc LC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency. Peaks tr (min) (mg/ml). Uracil.44 0.0. Toluene 3.96 3.00 3. Naphthalene 4.4 0.50 4. Biphenyl 5.3 0.06 With guard column : Roc C8 (cat.# 9533565); Dimensions: 50 mm x 4.6 mm ID; ; Pore Size: 00 Å; Guard : Roc C8 0 mm, 4.0 mm ID, 5 µm (cat.# 9533500); ; : Custom mix; Diluent: 50:50 Acetonitrile:water; Inj. Vol.: µl; : A: Water, B: Acetonitrile; Without guard column Gradient (%B): 0.00 min (70%), 8.00 min (70%); Flow:.0 ml/min; : UV @ 54 nm; :. 3 k =.7 Asymmetry =.07 Plates/m = 97,5 4 k =.7 Asymmetry =.097 Plates/m = 9,053 LC_GN0565 Figure 3: From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again. Reversed Phase Peaks tr (min). p-tolunitrile 3.7. 4-Butylbenzoic acid 5. 3. 3,4-Dimethylanisole 7.3 4. Mefenamic acid 8. 5. p-xylene 9.7 3 4 5 Lot Peaks tr (min). Benzene 3.5. Nitrobenzene 4. 3.,3,5-Trinitrobenzene 5.5 4.,4-Dinitrotoluene 7.4 Normal Phase Lot 5 Lot Lot 0.0.5 5.0 7.5 0.0 5 Lot 3 LC_GN056 : Roc C8 (cat.# 9534565); Dimensions: 50 mm x 4.6 mm ID; ; Pore Size: 00 Å; Temp.: 35 C; Inj. Vol.: 5 µl; : 0.05% Formic acid in water:0.05% formic acid in acetonitrile (40:60); Flow:.0 ml/min; : UV/Vis @ 0 nm; Cell Temp: 35 C; :. Lot 3 0.0.5 5.0 7.5 0.0 LC_GN0563 : Roc Cyano (cat.# 95363E); Dimensions: 00 mm x 3.0 mm ID; Particle Size: 3 µm; Pore Size: 00 Å; Temp.: 40 C; : Inj. Vol.: µl; : Water:acetonitrile (80:0); Flow: 0.6 ml/min; : UV/Vis @ 54 nm; Cell Temp.: 40 C; :. Find the entire Roc column line and order now at www.restek.com/roc www.restek.com 3
Roc LC s from Restek Perform Better Than or Equal To s from Other Vendors Table I: Roc LC columns offer equivalent or better peak shape, retention, and resolution compared to FPP columns from other manufacturers under identical conditions (average values, n = 5). s Analyte tr Area Height USP Tailing Asymmetry (0%) k' Plate Number Resolution Ethylparaben 3.5,407,609 8,358.76.48.8 9,738 Roc 5.40,869,684 34,346.075.064.63 0,64.5 Ethylparaben 3.060,44,6 5,698.907.608.03 6,73 Vendor A 4.889,860,659 3,879.3.7.7 8,575 0. Ethylparaben.873,46,068 39,850.43.4 0.866 9,706 Vendor B 4.307,867,049 87,44.064.057.797 0,03 9.9 Ethylparaben.763,43,099 35,080.6.7 0.93 8,643 Vendor C 4.580,868,74 70,66.43..03 9,700.9 Ethylparaben 3.57,939,87 357,54.59 0.965.084 4,478 Vendor D 5.86,77,785 8,056.30.3.839 8,954. Vendor E Ethylparaben 3.873,498,446 44,64.7.34. 9,348 6.839,78,453 74,955.080.063.9 9,963 3.7 Chromatographic conditions for Table I and Figure 4 (USP Monograph 38, ) All columns are C8 stationary phase (5 µm, 50 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (:); Flow rate: ml/min; Injection volume: 5 µl; : with UV detector, 80 nm; : 00 µg/ml ethinyl estradiol and 50 µg/ml ethylparaben in water:acetonitrile (:). 4 www.restek.com
Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Roc LC. Ethylparaben 3.5 50. estradiol 5.40 00 Roc C8 (cat.# 9534565) Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX058 Vendor A. Ethylparaben 3.060 50. estradiol 4.889 00 Vendor C C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX05 www.restek.com 5
(Continued from page 5) Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Vendor B. Ethylparaben.873 50. estradiol 4.307 00 Vendor B C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 0 Å Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX050 Vendor C. Ethylparaben.763 50. estradiol 4.580 00 Vendor A C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 80 Å Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX059 6 www.restek.com
(Continued from page 6) Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Vendor D. Ethylparaben 3.57 50. estradiol 5.86 00 Vendor D C8 Dimensions: 50 mm x 4.6 mm ID Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX05 Vendor E. Ethylparaben 3.873 50. estradiol 6.839 00 Vendor E C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A 0.00.0 00 8.00.0 00 UV @ 80, 4 nm 0.0 0.5.0.5.0.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 LC_EX053 www.restek.com 7