Datasheet GeneTex, Inc : Toll Free 1-877-GeneTex (1-877-436-3839) Fax:1-949-309-2888 info@genetex.com GeneTex International Corporation : Tel:886-3-6208988 Fax:886-3-6208989 infoasia@genetex.com Date : 2016/4/11 Catalog Number GTX107678 Package:25 μl,100 μl ( 7 ) Product Name Full Name Synonyms Product Description Background Host Clonality Isotype Immunogen Antigen Species Species Reactivity Predicted Cross Reactivity species NFkB p65 antibody v-rel reticuloendotheliosis viral oncogene homolog A (avian) MGC131774 antibody, NFKB3 antibody, p65 antibody, RELA antibody, nuclear factor NF-kappa-B p65 subunit antibody, transcription factor p65 antibody, NF-kappa-B p65delta3 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, v- rel reticuloendotheliosis viral oncogene homolog A (avian) antibody Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian)) NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa- B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-b-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] Rabbit Polyclonal IgG Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary. Human Human, Mouse Rat, Xenopus Tropicalis, Pig, Chimpanzee, Bovine Predict Reactivity Note Rat (99%), Xenopus Tropicalis (80%), Pig (98%), Chimpanzee (100%), Bovine (100%) Applications EMSA, ICC/IF, IHC-P, IP, WB Application Note Positive Controls Predicted Target Size Cellular Localization Conjugation Form Supplied Purification Concentration Storage Buffer Suggested dilution Electrophoretic mobility shift assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:500-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher. Molt-4, Raji, GL261, C8D30, NIH-3T3, 293T, A431, K562, GFP-human NF-kappa-B-transfected 293T 60 kda Nucleus, Cytoplasm Unconjugated Liquid Purified by antigen-affinity chromatography. 1 mg/ml (Please refer to the vial label for the specific concentration) 1XPBS, 20% Glycerol (ph7). 0.025% ProClin 300 was added as a preservative. Reference
Storage Buffer Storage Instruction Notes ResearchArea 1XPBS, 20% Glycerol (ph7). 0.025% ProClin 300 was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Cancer > Apoptosis > Anti-apoptosis Cancer > Type of cancer > Breast > Other Cancer > Type of cancer > Prostate cancer Application Reference 1. Raninga PV (2015) Oncotarget 2. Chen T (2014) Exp Dermatol 3. Liu J (2014) Arch Anim Nutr 111-25 4. Liang CJ (2014) Evidence-Based Complementary and Alternative Medicine 13 5. Adrianto I (2011) Nat Genet 253-8 6. Hsieh JY (2010) Stem Cells Dev 1895-910 7. Liang CJ (2011) Free Radic Biol Med 1337-46 GTX107678 IP Image NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent. A. 30 μg GL261 whole cell lysate/extract
A. 30 μg GL261 whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract 10 % SDS-PAGE A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE A. 30 μg K562 whole cell lysate/extract 7.5 % SDS-PAGE Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000.
GTX107678 ICC/IF Image NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining. GTX107678 IHC-P Image Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 (GTX107678) antibody at 1:500 dilution. Sample(30 ug whole cell lysate)
Sample(30 ug whole cell lysate) A:MOLT4 (GTX27912) 7.5% SDS PAGE GTX107678 diluted at 1:1000 GTX107678 ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(gtx107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.