ACE ENVIRONMENTAL 300 BELLA HARBOR CT #111 PALM COAST, FL 32137 Certificate of Mold Analysis Prepared for: ACE ENVIRONMENTAL Phone Number: (904) 859-9211 Fax Number: Project Name: Test Location: 1745 WINDSONG CIR. FLAGLER BEACH, FL 32136 Chain of Custody #: 46 Received Date: November 18, 2014 Report Date: November 19, 2014 Erika Piechowski, Technical Manager Carlos Ochoa, Quality Control Manager Currently there are no Federal regulations for evaluating potential health effects of fungal contamination and remediation. This information is subject to change as more information regarding fungal contaminants becomes available. For more information visit http://www.epa.gov/mold or www.nyc.gov/html/doh/html/epi/mold.shtml. This document was designed to follow currently known industry guidelines for the interpretation of microbial sampling, analysis, and remediation. Since interpretation of mold analysis reports is a scientific work in progress, it may as such be changed at any time without notice. The client is solely responsible for the use or interpretation. PRO-LAB/SSPTM Inc. makes no express or implied warranties as to health of a property from only the samples sent to their laboratory for analysis. The Client is hereby notified that due to the subjective nature of fungal analysis and the mold growth process, laboratory samples can and do change over time relative to the originally sampled material. PRO-LAB/SSPTM Inc. reserves the right to properly dispose of all samples after the testing of such samples are sufficiently completed or after a 7 day period, whichever is greater. For more information please contact PRO-LAB at (954) 384-4446 or email info@prolabinc.com Page 1 of 9
0 Prepared for : ACE ENVIRONMENTAL Test Address : IDENTIFICATION Mold Present Mold Present 1745 WINDSONG CIR. FLAGLER BEACH, FL 32136 ANALYSIS METHOD Direct Microscopic Exam Direct Microscopic Exam Spore trap analysis Spore trap analysis LOCATION KITCHEN AC REGISTER 1/2 BATH FAN MASTER BATHROOM MASTER BATHWALL CAVITY COC / LINE # 46-1 46-2 46-3 46-4 SAMPLE TYPE & VOLUME SWAB SWAB Z5-25L Z5-10L SERIAL NUMBER None supplied None supplied Q205426 Q205438 COLLECTION DATE Nov 17, 2014 Nov 17, 2014 Nov 17, 2014 Nov 17, 2014 ANALYSIS DATE Nov 19, 2014 Nov 19, 2014 Nov 19, 2014 Nov 19, 2014 CONCLUSION UNUSUAL UNUSUAL NOT ELEVATED NOT ELEVATED Bipolaris/Drechslera 3 21 Cercospora Chaetomium 1 100 17 Cladosporium X 1 7 Coelomycetes Curvularia 2 14 Epicoccum Ganoderma Hyphae X X Nigrospora Other Ascospores Other Basidiospores 3 21 3 300 50 Penicillium/Aspergillus 1 7 Pyricularia Scopulariopsis Smuts, myxomycetes 1 7 Unidentified X 3 21 2 200 33 TOTAL SPORES NA NA 14 560 100 6 600 100 MINIMUM DETECTION LIMIT * NA NA 1 1 100 BACKGROUND DEBRIS Not Applicable Not Applicable too heavy for accurate count. Heavy Cellulose Fiber Fiberglass 100 10,000 Plant Fragments Pollen OBSERVATIONS & COMMENTS Presence of current or former growth observed. Presence of current or former growth observed. s are estimated. Actual numbers of spores probably much higher. Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 2 of 9
0 Prepared for : ACE ENVIRONMENTAL Test Address : IDENTIFICATION 1745 WINDSONG CIR. FLAGLER BEACH, FL 32136 ANALYSIS METHOD Spore trap analysis Spore trap analysis Spore trap analysis INTENTIONALLY BLANK LOCATION MASTER BEDROOM KITCHEN OUTSIDE FRONT COC / LINE # 46-5 46-6 46-7 SAMPLE TYPE & VOLUME Z5-25L Z5-25L Z5-25L SERIAL NUMBER Q205472 Q203647 Q203632 COLLECTION DATE Nov 17, 2014 Nov 17, 2014 Nov 17, 2014 ANALYSIS DATE Nov 19, 2014 Nov 19, 2014 Nov 19, 2014 CONCLUSION NOT ELEVATED NOT ELEVATED CONTROL Bipolaris/Drechslera 10 0 27 6 2 15 3 1 Cercospora 3 1 Chaetomium Cladosporium 7 2 19 23 920 58 229 9,200 84 Coelomycetes 1 3 Curvularia 1 3 2 5 1 <1 Epicoccum 1 <1 Ganoderma 1 3 1 <1 Hyphae Nigrospora 1 3 2 1 Other Ascospores 1 3 1 3 4 1 Other Basidiospores 11 4 30 2 5 13 520 5 Penicillium/Aspergillus 7 2 3 Pyricularia 2 1 Scopulariopsis 1 3 Smuts, myxomycetes 1 3 1 <1 Unidentified 4 11 4 10 6 2 2 TOTAL SPORES 37 1,4 100 1,600 100 273 10,960 100 MINIMUM DETECTION LIMIT * 1 1 1 BACKGROUND DEBRIS Moderate Moderate Light Cellulose Fiber 14 560 6 2 Fiberglass 1 1 2 Plant Fragments 1 4 Pollen 1 1 2 OBSERVATIONS & COMMENTS Debris mostly non-biological Debris mostly non-biological Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 3 of 9
Chain of Custody # 46 Master Bathroom Outside Front Unidentified 2 Sm uts, m y x om y c etes Py ric ularia Penic illium /As pergillus 2 Other Bas idios pores 520 Other As c os pores Nigros pora Ganoderm a Epic oc c um Curv ularia Clados porium 9200 Cerc os pora Bipolaris /Drec hs lera 1 10 100 1000 10000 per cubic meter Page 4 of 9
Chain of Custody # 46 Master Bathwall Cavity Outside Front Unidentified 2 200 Sm uts, m y x om y c etes Py ric ularia Penic illium /As pergillus 2 Other Bas idios pores 300 520 Other As c os pores Nigros pora Ganoderm a Epic oc c um Curv ularia Clados porium 9200 Chaetom ium Cerc os pora Bipolaris /Drec hs lera 100 1 10 100 1000 10000 per cubic meter Page 5 of 9
Chain of Custody # 46 Master Bedroom Outside Front Unidentified 2 Sm uts, m y x om y c etes Py ric ularia Penic illium /As pergillus 2 Other Bas idios pores 520 4 Other As c os pores Nigros pora Ganoderm a Epic oc c um Curv ularia Coelom y c etes Clados porium 2 9200 Cerc os pora Bipolaris /Drec hs lera 0 1 10 100 1000 10000 per cubic meter Page 6 of 9
Chain of Custody # 46 Kitchen Outside Front Unidentified 2 Sm uts, m y x om y c etes Sc opulariops is Py ric ularia Penic illium /As pergillus 2 Other Bas idios pores 520 Other As c os pores Nigros pora Ganoderm a Epic oc c um Curv ularia Clados porium 920 9200 Cerc os pora Bipolaris /Drec hs lera 2 1 10 100 1000 10000 per cubic meter Page 7 of 9
Identification Outdoor Habitat Indoor Habitat Possible Allergic Potential Not an opinion or interpretation Bipolaris/Drechslera Cercospora Chaetomium Cladosporium Coelomycetes Curvularia Epicoccum Ganoderma Common everywhere. Frequently associated with grasses, but also found on plant material, decaying food, and soil. Common everywhere, especially growing on leaves. Growing on dung, dead leaves, wood. The most common spore type reported in the air worldwide. Found on dead and dying plant litter, and soil. Commonly found everywhere growing on plants and animals. Commonly found everywhere on soil and plant debris. Commonly found everywhere. Grows on plant debris, insects and soil. Common everywhere growing on hardwood trees. Not known to grow indoors. None known. Cellulose substrates, especially wallboard, cardboard and wood. Not normally seen growing indoors unless the building material has been wetted. Unusual / Not Normal to be growing indoors. Commonly found on wood and wallboard. Commonly grows on window sills, textiles and foods. Can grow on ceiling tiles, wood, paper Capable of growing on many cellulytic substrates like wallboard and wood. Capable of growing on several different substrates, notably wallboard and paper. None known. None known. Hyphae Common everywhere. All substrates. None known. Nigrospora Commonly found everywhere. Grows on decaying plant material Does not normally grow on building materials, but occasionally can be found growing on wallboard. Common Type I (hay fever and asthma), fungal sinusitis., Type III (hypersensitivity pneumonitis) and common cause of allergenic sinusitis. Comments This is a group of like-looking spores that include Bipolaris, Drechslera, Exserohilum, and sometimes Helminosporium. They cannot be consistently separated by spore morphology and are thus grouped together. Must be cultured to consistly separate the genera. Chaetomium is a water-indicating mold. of this type of mold should not be observed in significantly higher numbers in the air above background/control. If growth and/or significantly higher than backgroud/control spore numbers are reported, corrective action should be considered to reduce the source of water, moisture levels and/or spore numbers in the living space. A very common and important allergen source both outdoors and indoors. Rarely reported in the air becuase they are formed in fruiting bodies and generally slimy and therefore, difficult to be sent airborne. Very common in the summer, especially in the midwest and during harvest time. Hyphae are the "root-like" food absorption strands common to nearly all fungi. They sometimes can become airborne. Very distinctive spore that is easy to identify. Page 8 of 9
Identification Outdoor Habitat Indoor Habitat Possible Allergic Potential Not an opinion or interpretation Common everywhere. Constitutes a large part of the Ascospores airspora outside. Can reach Very few of this group grow Little known for most of this group of very high numbers in the air inside. The notable exception is fungi. Dependent on the type (see outside during the spring and Chaetomium, Ascotricha and Chaetomium and Ascotricha). summer. Can increase in Peziza. numbers during and after rainfalls. Basidiospores Penicillium/Aspergillus Pyricularia Scopulariopsis Smuts, myxomycetes Unidentified Commonly found everywhere, especially in the late summer and fall. These spores are from Mushrooms. Common everywhere. Normally found in the air in small amounts in outdoor air. Grows on nearly everything. Common everywhere. Grows on grass leaves. Common everywhere. Mostly reported from soil, dung, and fingernails. Commonly found everywhere, espcially on logs, grasses and weeds. Common everywhere. Grow on decaying plant litter and other plant-derived material. Mushrooms are not normally found growing indoors, but can grow on wet lumber, especially in crawlspaces. Sometimes mushrooms can be seen growing in flower pots indoors. Wetted wallboard, wood, food, leather, etc. Able to grow on many substrates indoors. Not known to grow indoors. None known. Wetted wallboard, wood, and paper products. Smuts don`t normally grow indoors, but can occasionally be found on things brought from outside and stored in the house. Myxomycetes can occasionally grow indoors, but need lots of water to be established. Wetted cellulosic material. Some allergenicity reported. Type I (hay fever, asthma) and Type III (hypersensitivity pneumonitis). allergies and Type III (hypersensitivity pneumonitis) Very little is known regarding allergic potential, but should be considered similar to Penicillium and Aspergillus because it has a similar sized spore. None known. Comments Among the group of Mushrooms (Basidiomycetes) are dry rot fungi Serpula and Poria that are particularly destructive to buildings. This is a combination group of Penicillium and Aspergillus and is used when only the spores are seen. The spores are so similar that they cannot be reliably separated into their respective genera. Smuts and myxomycetes are a combined group of organisms because their spores look so similar and cannot be reliably distinquished from each other. This group of spores is reserved for spores whose identity is unknown. These kinds of spores have usually never been seen before in spore traps by our laboratory and/or are of such morphology that they cannot be identified with any degree of certainty to a particular genus. Page 9 of 9